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The document outlines various blood withdrawal techniques commonly used in laboratory rodents, including retro-orbital, tail snip, intracardiac, and posterior vena cava methods. Each technique is detailed with specific procedures to ensure proper and humane blood collection. Additional methods such as saphenous, submandibular, jugular vein, and dorsal metatarsal vein sampling are also mentioned.

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Mohammad Altaf
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4 views

experiment - y

The document outlines various blood withdrawal techniques commonly used in laboratory rodents, including retro-orbital, tail snip, intracardiac, and posterior vena cava methods. Each technique is detailed with specific procedures to ensure proper and humane blood collection. Additional methods such as saphenous, submandibular, jugular vein, and dorsal metatarsal vein sampling are also mentioned.

Uploaded by

Mohammad Altaf
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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Supportive Resources-4

Aim/Object: Commonly used blood withdrawal techniques in laboratory animals


Reference: “Ex Pharm” Software by “Bureau for Health & Education Status
Upliftment”, assessed at: https://heb-nic.in/Ex-Pharm/login.php
Introduction:
The most commonly used blood withdrawal methods in rodents are:
- Retro-orbital sinus blood withdrawal method.
- Tail snip blood withdrawal method

- Tail blood withdrawal from tail.


- Intracardiac blood withdrawal
- Posterior vena cava blood withdrawal
- Saphenous Sampling (Medial or Lateral Approach)
- Submandibular Sampling
- Jagular Vein Sampling
- Dorsal Metatarsal Vein

Retro-orbital blood withdrawal method


Procedure:
1. The animal is anesthetized with an inhalation anaesthetic, such as isoflurane, in a bell jar.
2. Once the animal is fully anesthetized. The eye is protruded by placing a finger on the top of
the head and along the jawline.
3. The capillary tube is placed in the medial part of the eye at a 40-450 angle.
4. Apply pressure by gently rotating the hematocrit tube. This will cut through the conjunctival
membranes.
5. The blood will flow into the hematocrit tube by capillary action.
6. Once blood begins to flow, maintain pressure to keep the eye protruded.
7. After collection completes, to stop bleeding, release the skin and allow the eye to return to the
normal position.

Tail snip blood withdrawal procedure


Procedure:

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1. The animal is placed into the restraint tube such that tail is accessible.
2. The tail is wiped with slight warm water.

3. For the tail snip, the tail is extended, and the end of the tail i.e. 0.5-1 mm in case of mice and
up to 2 mm in case of rats, is cut with the scalpel (10-15 Number)
4. The cut is made over the lateral tail vein.
5. The tail can be stroked from rear end of tip to encourage blood flow.

6. The blood is collected from the tip using hematocrit tubes blood allowed to drip from tail
directly into a collection vial.

Tail blood withdrawal from tail


Procedure:
1. The animal is placed into the restraint tube such that the tail is accessible.
2. The tail is wiped with slight warm water.

3. For the blood withdrawal, the tail is extended and the blood is collected by syringe.
4. The tail can be stroked from rear end of tip to encourage blood flow.
5. The blood is collected in the syringe.

Cardiac Blood Collection


Procedure:
1. The animal is restrained by the scruff with the body hanging vertically.
2. The needle is protruded in the notch just to the left of the animal's xiphoid.
3. The needle shall be kept parallel to the spine and placed just under the ribs.

4. Place the needle, protrude up, into the chest, and puncture the heart.
5. Apply slight pressure through the syringe. If the needle is in the heart, blood will flow
automatically into the syringe.
6. Wait until the blood gets filled in the syringe before adding more pressure on the syringe.
*For blood collection from a mouse, a 3 cc syringe with a 22-25 gauge x 1" needle is preferred.
*For blood collection from a rat, a 10-12 cc syringe with an 18 gauge x 1.5" needle is preferred.

Posterior vena cava blood withdrawal


Procedure:
1. Animal is placed on surgical platform, dissection tray, injectable anesthesia or inhalation
anesthesia is applied.

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2. It is ensured that animal is completely anesthetized, as determined by toe pinch or tail pinch.
3. The skin is lifted and a small transverse cut is made through the skin just above the pelvis in
females, or just above the prepuce in males.
4. The muscle is lifted, and a small transverse cut is made through the muscle just above the
skin cut.
5. Cut transversely along the side of the ribs on both sides.
6. Gently protrude the intestines to the animal's left to expose the posterior vena cava.

7. Insert the needle; protrude upward, into the vena cava midway between the joint of the renal
vessels and the iliac bifurcation.
8. Slowly withdraw the blood by applying pressure on the liver.

Miscellaneous
In addition to methods discussed above following methods are also used for blood withdrawal
1. Saphenous Sampling (Medial or Lateral Approach: In this method blood is collected from
the Saphenous Vein of the leg of the rodent.
2. Submandibular Sampling: In this method blood is collected from Submedibular Vein of the
dental region.
3. Jugular Vein Sampling: Blood is collected from the Jugular Vein of the neck region.

4. Dorsal Metatarsal Vein: Blood is collected from metatarsal region; this method is preferred
for Rats over mice.

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