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The
IMAGE
PROCESSING
Handbook
Sixth Edition
John C. Russ
The
IMAGE
PROCESSING
Handbook
Sixth Edition
The
IMAGE
PROCESSING
Handbook
Sixth Edition
John C. Russ
North Carolina State University
Materials Science and Engineering Department
Raleigh, North Carolina
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Contents
Acknowledgments. . . . . . . . . . . . . . . . . . . xi
Introduction . . . . . . . . . . . . . . . . . . . . . . xiii
Author . . . . . . . . . . . . . . . . . . . . . . . . . xvii
1 Acquiring Images . . . . . . . . . . . . . . . . 1
Human reliance on images for information............ 1
Video cameras................................................... 6
CCD cameras.................................................... 7
Camera artifacts and limitations..........................13
Color cameras..................................................15
Camera resolution.............................................18
Focusing.......................................................... 20
Electronics and bandwidth limitations...................21
Pixels...............................................................24
Gray scale resolution........................................ 26
Noise............................................................. 28
High depth images........................................... 30
Color imaging...................................................34
Digital camera limitations.................................. 42
Color spaces................................................... 42
Color correction................................................52
Color displays...................................................54
Image types..................................................... 56
Range imaging................................................ 58
Multiple images............................................... 64
Stereoscopy..................................................... 69
Imaging requirements........................................ 77
v
© 2011 by Taylor & Francis Group, LLC
2 Human Vision. . . . . . . . . . . . . . . . . . 85
What we see and why...................................... 85
Recognition..................................................... 88
Technical specs................................................ 92
Acuity..............................................................97
What the eye tells the brain..............................101
Spatial comparisons........................................103
Local to global hierarchies................................107
It’s about time................................................. 113
The third dimension.......................................... 118
How versus what............................................. 121
Seeing what isn’t there, and vice versa...............122
Image compression..........................................125
A world of light...............................................126
Size matters....................................................129
Shape (whatever that means)............................132
Context..........................................................133
Arrangements must be made.............................135
Seeing is believing.......................................... 137
So in conclusion..............................................139
vi Contents
© 2011 by Taylor & Francis Group, LLC
Defect removal, maximum entropy, and
maximum likelihood.........................................232
Nonuniform illumination...................................235
Fitting a background function............................238
Rank leveling..................................................244
Color images..................................................248
Non-planar views............................................250
Computer graphics..........................................252
Geometric distortion........................................254
Alignment.......................................................256
Interpolation...................................................261
Morphing.......................................................265
Contents vii
© 2011 by Taylor & Francis Group, LLC
7 Segmentation and Thresholding. . . .395
Thresholding...................................................395
Automatic settings...........................................398
Multiband images.......................................... 403
Two-dimensional thresholds.............................. 405
Multiband thresholding................................... 408
Thresholding from texture.................................. 411
Multiple thresholding criteria............................. 414
Textural orientation.......................................... 415
Region boundaries.......................................... 419
Conditional histograms.....................................426
Boundary lines................................................427
Contours....................................................... 430
Image representation.......................................432
Other segmentation methods............................436
The general classification problem.................... 440
viii Contents
© 2011 by Taylor & Francis Group, LLC
Determining number........................................532
Curvature, connectivity, and the Disector............535
Anisotropy and gradients.................................538
Size distributions.............................................542
Classical stereology (unfolding).........................543
Contents ix
© 2011 by Taylor & Francis Group, LLC
Beam hardening............................................. 686
Imaging geometries.........................................691
Three-dimensional tomography..........................695
High-resolution tomography..............................701
14 3D Visualization . . . . . . . . . . . . . . . 707
Sources of 3D data..........................................707
Serial sections.................................................709
Optical sectioning........................................... 713
Sequential removal.......................................... 715
Stereo measurement........................................ 717
3D data sets...................................................722
Slicing the data set..........................................724
Arbitrary section planes...................................727
The use of color.............................................. 731
Volumetric display...........................................732
Stereo viewing................................................736
Special display hardware.................................739
Ray tracing..................................................... 741
Reflection.......................................................746
Surfaces.........................................................750
Multiply connected surfaces..............................754
Image processing in 3D...................................759
Measurements on 3D images............................763
References . . . . . . . . . . . . . . . . . . . . . . 817
x Contents
© 2011 by Taylor & Francis Group, LLC
Acknowledgments
All of the image processing and the creation of the resulting figures included in this
book were performed on an Apple Macintosh® and/or a Sony VAIO® computer, using
Adobe Photoshop® CS4 with the Fovea Pro plug-ins. Many of the images were acquired
directly from various microscopes and other sources that provided digital output directly
to the computer. Others were captured using a variety of digital cameras (Sony, Nikon,
Canon, and others), and some were obtained using flat-bed and slide scanners (Nikon and
Epson), often from images supplied by colleagues and researchers. These are acknowl-
edged wherever the origin of an image could be determined. A few examples, taken
from the literature, are individually referenced.
The book was delivered to the publisher in digital form (on a writable DVD), without
intermediate hard copy, negatives or prints of the images, etc. Among other things,
this means that the author must bear full responsibility for typographical errors or
problems with the figures. Every effort has been made to show enlarged image frag-
ments that will reveal pixel-level detail when it is important. The process has also
forced me to learn more than I ever hoped to know about some aspects of publish-
ing technology! However, going directly from disk file to print also shortens the time
needed in production and helps to keep costs down, while preserving the full quality
of the images. Grateful acknowledgment is made of the efforts by the excellent edi-
tors at CRC Press to educate me and to accommodate the unusually large number of
illustrations in this book (more than 2000 figures and more than a quarter of a mil-
lion words).
Special thanks are due to Chris Russ (Reindeer Graphics Inc., Asheville, NC) who has
helped to program many of these algorithms and contributed invaluable comments, and
especially to Helen Adams, who has proofread many pages, endured many discussions
about ways to present information effectively, and provided the support (and the occa-
sional glass of wine) that make writing projects such as this possible.
John C. Russ
Raleigh, NC
xi
© 2011 by Taylor & Francis Group, LLC
Introduction
Image processing is used in a wide variety of applications, for two somewhat differ-
ent purposes:
The techniques that are appropriate for each of these tasks are not always the same, but
there is considerable overlap. This book covers methods that are used for both tasks.
To do the best possible job, it is important to know about the uses to which the pro-
cessed images will be put. For visual enhancement, this means having some familiarity
with the human visual process and an appreciation of what cues the viewer responds
to in images. A chapter on human vision addresses those issues. It also is useful to
know about the printing or display process, since many images are processed in the
context of reproduction or transmission. Printing technology for images has advanced
significantly with the consumer impact of digital cameras, and up-to-date information
is provided.
The measurement of images is often a principal method for acquiring scientific data and
generally requires that features or structure be well defined, either by edges or unique
brightness, color, texture, or some combination of these factors. The types of measure-
ments that can be performed on entire scenes or on individual features are important in
determining the appropriate processing steps. Several chapters deal with measurement
in detail. Measurements of size, position, and brightness deal with topics that humans
largely understand, although human vision is not quantitative and is easily fooled. Shape
is a more difficult concept, and a separate chapter added in this edition summarizes a
variety of ways that shape may be described by numbers. Measurement data may be
used for classification or recognition of objects. There are several different strategies that
can be applied, and examples are shown.
It may help to recall that image processing, like food processing or word processing,
does not reduce the amount of data present but simply rearranges it. Some arrangements
xiii
© 2011 by Taylor & Francis Group, LLC
may be more appealing to the senses, and some may convey more meaning, but these
two criteria may not be identical nor call for identical methods.
This handbook presents an extensive collection of image processing tools, so that the
user of computer-based systems can both understand those methods provided in pack-
aged software and program those additions which may be needed for particular applica-
tions. Comparisons are presented for different algorithms that may be used for similar
purposes, using a selection of representative pictures from various microscopy tech-
niques, as well as macroscopic, remote sensing, and astronomical images. It is very
important to emphasize that the scale of the image matters very little to the techniques
used to process or analyze it. Microscopes that have a resolution of nm and telescopes
that produce images covering light years produce images that require many of the same
algorithms.
The emphasis throughout the book continues to be on explaining and illustrating meth-
ods so that they can be clearly understood, rather than providing dense mathematics.
With the advances in computer speed and power, tricks and approximations in search
of efficiency are less important, so that examples based on exact implementation of
methods with full precision can generally be implemented on desktop systems. The top-
ics covered are generally presented in the same order in which the methods would be
applied in a typical workflow.
For many years, in teaching this material to students I have described achieving mastery of
these techniques as being much like becoming a skilled journeyman carpenter. The num-
ber of distinct woodworking tools — saws, planes, drills, etc. — is relatively small, and
although there are some variations — slotted vs. Phillips-head screwdrivers, for example
— knowing how to use each type of tool is closely linked to understanding what it does.
With a set of these tools, the skilled carpenter can produce a house, a boat, or a piece of
furniture. So it is with image processing tools, which are conveniently grouped into only
a few classes, such as histogram modification, neighborhood operations, Fourier-space
processing, and so on, and can be used to accomplish a broad range of purposes. Visiting
your local hardware store and purchasing the appropriate tools do not provide the skills
to use them. Understanding their use requires practice, which develops the ability to visu-
alize beforehand what each will do. The same is true of the tools for image processing.
In revising the book for this new edition, I have again tried to respond to some of the
comments and requests of readers and reviewers. New chapters on the measurement
of images and the subsequent interpretation of the data were added in the second edi-
tion, and a section on surface images in the third. The fourth edition added the stereo-
logical interpretation of measurements on sections through three-dimensional structures
and the various logical approaches to feature classification. The fifth edition brought
expanded sections on deconvolution, extended dynamic range images, and multichannel
imaging, including principal components analysis. In this sixth edition, a new chapter on
the meaning of shape has been added, as well as additional material on imaging in more
than two dimensions. The sections on the ever-advancing hardware for image capture
and printing have been expanded and information added on the newest hardware and
software technologies.
As in past editions, I have resisted suggestions to put “more of the math” into the book.
There are excellent texts on image processing, compression, mathematical morphol-
ogy, etc., that provide as much rigor and as many derivations as may be needed. Many
of them are referenced here. But the thrust of this book remains teaching by example.
xiv Introduction
© 2011 by Taylor & Francis Group, LLC
Few people learn the principles of image processing from the equations. Just as we use
images to communicate ideas and to “do science,” so most of us use images to learn
about many things, including imaging itself. The hope is that by seeing and comparing
what various operations do to representative images, you will discover how and why to
use them. Then, if you need to look up the mathematical foundations, they will be easier
to understand.
A very real concern for everyone involved in imaging, particularly in scientific and foren-
sic fields, is the question of what constitutes proper and appropriate processing and what
constitutes unethical or even fraudulent manipulation. The short answer is that anything
that alters an image so as to create a false impression on the part of the viewer is wrong.
The problem with that answer is that it does not take into account the fact that different
viewers will tend to see different things in the image anyway, and that what constitutes
a false impression for one person may not for another.
The first rule is always to store a permanent copy of the original image along with rel-
evant data on its acquisition. The second rule is to carefully document whatever steps
are taken to process the image and generally to report those steps when the processed
image is published. Most scientific publications and the editors who review submitted
papers have become more aware in recent years of the ease with which image process-
ing can be performed and the dangers of inadequate documentation. For example, see
M. Rossner and K. M. Yamada (2004; J. Cell Biology) for that journal’s policy on image
ethics and examples of improper manipulation.
For forensic purposes, there is an additional responsibility to fully record the entire step-
by-step procedures that are used and to make sure that those methods are acceptable
in court according to the U.S. Supreme Court’s Daubert ruling (Daubert v. Merrell Dow
Pharmaceuticals (92-102), 509 U.S. 579, 1993), which generally means that not only are
the methods widely accepted by professionals, but also that they have been rigorously
tested and have known performance outcomes. In a forensic setting, there will often
be a need to explain a procedure, step by step, to a non-technical jury. This frequently
requires showing that the details obtained from the image are really present in the origi-
nal but only became visually evident with the processing.
Some procedures, such as rearranging features or combining them within a single image,
or differently adjusting the contrast of several images to make them appear more alike,
are clearly misleading and generally wrong. Some, such as using copy-and-paste to dupli-
cate a portion of an image, or selectively erasing portions of an image, are out-and-out
fraudulent. Even selective cropping of an image (or choosing which field of view to
record) can create a false impression.
The general guideline to be considered is that it is never acceptable to add anything to
an image, but it may be acceptable to suppress or remove some information if it makes
the remaining details more accessible, either visually for presentation and communication
or to facilitate measurement. Of course, the procedures used must be documented and
reported. Any of the procedures shown here may be appropriate in a particular instance.
But they can also be misused and should in any case never be used without understand-
ing and careful documentation. The heart of the scientific method is replicability. If
adequate information is provided on the processing steps applied and the original image
data are preserved, then the validity of the results can be independently verified.
An important but often overlooked concern is the need to avoid using programs that
alter the image without the user being aware of it. For example, carefully correcting the
Introduction xv
© 2011 by Taylor & Francis Group, LLC
colors in an image using Photoshop® and then placing it in PowerPoint® for presentation
will cause changes even on the same computer screen (as well as discarding pixels and
reducing resolution if copy-and-paste is used for the transfer). In addition, the image
may appear different on another computer monitor or when using a projector. Pasting an
image into Microsoft® Word will reduce the resolution and color or gray scale dynamic
range. This may not affect the printed document, which has less gamut than the com-
puter screen anyway, but the image cannot be subsequently retrieved from the document
in its original form. Saving an image with a lossy compression method such as jpeg will
discard potentially important information that cannot be recovered.
The reader is encouraged to use this book in concert with a real source of images and
a computer-based system and to freely experiment with different methods to deter-
mine which are most appropriate for his or her particular needs. Selection of image
processing tools to explore images when you don’t know the contents beforehand is
a much more difficult task than using tools to make it easier for another viewer or a
measurement program to see the same things you have discovered. It places greater
demand on computing speed and the interactive nature of the interface. But it particu-
larly requires that you become a very analytical observer of images. If you can learn
to see what the computer sees and predict what various algorithms will do, you will
become a better viewer and obtain the best possible images, suitable for further pro-
cessing and analysis.
To facilitate this hands-on learning, I have collaborated with my son, Chris Russ, to
write a companion book, Introduction to Image Processing and Analysis, which teaches
how to program these algorithms and create Adobe Photoshop compatible plug-ins that
implement the methods. The downloadable solutions to the book’s worked problems
can be used to apply the routines on either Macintosh or Windows computers. There are
additional links to downloadable plug-ins and trial program packages on my Web site at
http://www.DrJohnRuss.com.
xvi Introduction
© 2011 by Taylor & Francis Group, LLC
Author
xvii
© 2011 by Taylor & Francis Group, LLC
workshops worldwide on image processing and analysis, and to review publications and
funding proposals. He is active in the Microscopy Society of America, the Microbeam
Analysis Society, the Society of Photo-Optical Instrumentation Engineering (SPIE), the
International Society for Stereology, is a board member of the Society for Quantitative
Morphology, and a Fellow of the Royal Microscopical Society, and has presented invited
lectures and workshops for these and other organizations. On November 16, 2006, the
New York Microscopical Society awarded John Russ the Ernst Abbe Memorial Award for
his contributions to the field of microscopy as a developer of computer-assisted micros-
copy and image analysis.
xviii Author
© 2011 by Taylor & Francis Group, LLC
1
Acquiring Images
H
umans are primarily visual creatures. Not all animals depend on their eyes, as we do,
for most of the information received about their surroundings (the characteristics of
human vision are discussed in Chapter 2). This bias in everyday life extends to how
we pursue more technical goals as well. Scientific instruments commonly produce images
to communicate their results to the operator, rather than generating audible tones or emit-
ting a smell. Space missions to other planets and equally arduous explorations of the ocean
depths always include cameras as major components, and the success of those missions is
often judged by the quality of the images returned. This suggests a few of the ways in which
humans have extended the range of natural vision. Optical devices such as microscopes and
telescopes allow us to see things that are vastly smaller or larger than we could otherwise.
Beyond the visible portion of the electromagnetic spectrum (a narrow range of wavelengths
between about 400 and 700 nanometers) there are sensors capable of detecting infrared and
ultraviolet light, X-rays, and radio waves, and perhaps soon even gravity waves. Figure 1.1
shows an example, an image presenting radio telescope data in the form of an image in which
color represents the Doppler shift in the radio signal. Such devices and presentations are used
to further extend imaging capability.
Signals other than electromagnetic radiation can be used to produce images, too. Novel new
types of microscopes that use atomic-scale probes to “feel” the specimen surface present their
data as images (Figure 1.2). The data so collected may represent the surface elevation and
topography, but other signals, such as the lateral drag force on the probe, may also be used.
Acoustic waves at low frequency produce sonar images, while at gigahertz frequencies the
acoustic microscope produces images with resolution similar to that of the light microscope,
but with image contrast that is produced by local variations in the attenuation and refraction
of sound waves rather than light. Figure 1.3 shows an acoustic microscope image of a sub-
surface defect, and Figure 1.4 shows a sonogram of a baby in the womb.
Some images such as holograms or electron diffraction patterns record brightness as a func-
tion of position, but are unfamiliar to the observer. Figure 1.5 shows an image of an electron
diffraction pattern from a transmission electron microscope, in which the atomic structure of
the samples is revealed (but only by measurement and to those who know how to interpret
1
© 2011 by Taylor & Francis Group, LLC
Figure 1.1 Radio astronomy pro-
duces images such as this view of
Messier 33 (generated with data
from telescopes of the National
Radio Astronomy Observatory,
a National Science Foundation
Facility managed by Associated
Universities, Inc.). These are
often displayed with false colors
to emphasize subtle variations
in signal strength or - as in this
example - Doppler shift.
the data). Other kinds of data, including weather maps with specialized symbols, graphs of
business profit and expenses, and charts with axes representing time, family income, choles-
terol level, or even more obscure parameters, have become part of daily life, as illustrated in
Figure 1.6. The latest developments in computer interfaces and displays make extensive use
of graphics, to take advantage of the large bandwidth of the human visual pathway. Tufte
(1990, 1997, 2001) in particular has demonstrated the power of appropriate graphics to com-
municate complex information.
There are some important differences between human vision, the kind of information it
extracts from images, and the ways in which it seems to do so, as compared to the use of
imaging devices based on computers for scientific, technical, or forensic purposes. Humans
(b)
Figure 1.3 Acoustic microscope image of voids in solder bond beneath a GaAs die: (a) die surface; (b)
acoustic image showing strong signal reflections (white areas) from the surface of the voids (courtesy
J. E. Semmens, Sonoscan Inc).
are especially poor at judging color or brightness of objects and features within images unless
they can be exactly compared by making them adjacent. Human vision is inherently com-
parative rather than quantitative, responding to the relative size, angle, or position of several
objects but unable to supply numeric measures unless one of the reference objects is a mea-
suring scale. Overington (1976; 1992) disagrees with this widely accepted and documented
conclusion but presents no compelling counter evidence. Chapter 2 illustrates some of the
consequences of the characteristics of human vision as they affect what is perceived.
This book’s purpose is not to study the human visual pathway directly, but the overview in
Chapter 2 can help the reader to understand how humans see things so that we become bet-
ter observers. Computer-based image processing and analysis use algorithms based on human
vision methods in some cases, but also employ other methods that seem not to have direct
counterparts in human vision. In particular, some image processing methods are based on the
physics of the image formation and detection process (Sharma, 2005).
Many of the examples and much of the analysis presented in this text involve images from
various types of microscopes. The three classes of imaging applications that generally offer
Acquiring Images 3
© 2011 by Taylor & Francis Group, LLC
Figure 1.4 Surface reconstruction of sonogram imaging, showing a 26 week old fetus in the womb.
Figure 1.5 A convergent beam electron diffraction (CBED) pattern from an oxide microcrystal, which
can be indexed and measured to provide high accuracy values for the atomic unit cell dimensions.
the most straightforward types of images are microscopy, aerial (and satellite) imagery, and
industrial quality control. That is because in those situations there is the greatest knowledge
and/or control over the imaging geometry and the illumination of the scene. In more general
“real world” cases the analysis and interpretation of the image contents can be much more
difficult. Objects may lie at various distances from the camera, which complicates determin-
ing size, may have different lighting, which alters their color, and may even partially obscure
other objects. Crime scene and accident photographs are often taken under difficult condi-
tions, from less than optimum points of view, and with variable lighting, so that their analysis
can be challenging.
The basic techniques for image processing and measurement are much the same for images
regardless of their source or scale. Images ranging from microscopy to astronomy, images
formed with light photons or sound waves, magnetic resonance or scanning profilometers,
have much in common and the techniques for dealing with their imperfections, enhancing
and extracting the details, and performing measurements utilize the same algorithms and
techniques, which are set out in the following chapters. The interpretation of the measure-
ments, as presented in later chapters, does require some specialization for different viewing
geometries, but is fundamentally independent of magnification.
Acquiring Images 5
© 2011 by Taylor & Francis Group, LLC
Video cameras
When the first edition of this book was published in 1990, the most common and affordable
way of acquiring images for computer processing was with a video camera. Mounted onto a
microscope or copystand, in a satellite or space probe, or using appropriate optics to view
an experiment, the camera sent an analog signal to a separate “frame grabber” or analog-to-
digital converter (ADC) interface board in the computer, which then stored numeric values in
memory (Inoué, 1986; Inoué & Spring, 1997).
The basic form of the original type of video camera is the vidicon, illustrated in Figure 1.7. It
functions by scanning a focused beam of electrons across a phosphor coating applied to the
inside of an evacuated glass tube. The light enters the camera through the front glass surface
(and a thin metallic anode layer) and creates free electrons in the phosphor. These vary the
local conductivity of the layer, so the amount of current that flows to the anode varies as the
beam is scanned, according to the local light intensity. This analog (continuously varying)
electrical signal is amplified and, as shown in Figure 1.8, conforms to standards of voltage
and timing (the standards and timing are slightly different in Europe than the US, but the basic
principles remain the same).
Digitizing the voltage is accomplished by sampling it and generating a comparison voltage.
The child’s game of “guess a number” illustrates that it takes only eight guesses to arrive at a
Grid
Electron Beam
Cathode Glass Tube
Phosphor
Coating
Figure 1.7 Functional diagram of a vidicon tube. Light striking the phosphor coating changes its local
resistance and hence the current that flows as the electron beam scans in a raster pattern.
Figure 1.8 Standard RS-170 video signal shows the brightness variation along one scan line (ranging
between 0 volts = black and 0.7 volts = white).
value that defines the voltage to one part in 256 (the most widely used type of ADC). The first
guess is 128, or half the voltage range. If this is (e.g.) too large, the second guess subtracts
64. Each successive approximation adds or subtracts a value half as large as the previous. In
eight steps, the final (smallest) adjustment is made. The result is a number that is conveniently
stored in the 8-bit memory of most modern computers.
The tube-type camera has several advantages and quite a few drawbacks. Scanning the beam
with electromagnetic or electrostatic fields can produce a distorted scan (pincushion or barrel
distortion, or more complicated situations) and is subject to degradation by stray fields from
wiring or instrumentation. Figure 1.9 shows an example of pincushion distortion, as well
as vignetting and loss of focus. Maintaining focus in the corners of the image takes special
circuitry, and the corners may also be darkened (vignetting) by the reduction in effective lens
aperture and the additional thickness of glass through which the light must pass. The sealed
vacuum systems tend to deteriorate with time, and the “getter” used to adsorb gas molecules
may flake and fall onto the phosphor if the camera is used in a vertical orientation. The
response of the camera (voltage vs. brightness) approximates the logarithmic response of film
and the human eye, but this varies for bright and dark scenes. Recovery from bright scenes
and bright spots is slow, and blooming can occur in which bright light produces spots that
spread laterally in the coating and appear larger than the features really are, with “comet tails”
in the scan direction.
There are, however, some advantages of the tube-type camera. The spatial resolution is very
high, limited only by the grain size of the phosphor and the size of the focused beam spot.
Also, the phosphor has a spectral response that can be made similar to that of the human eye,
which sees color from red (about 0.7 µm wavelength) to blue (about 0.4 µm). Adaptations of the
basic camera design with intermediate cathode layers or special coatings for intensification are
capable of acquiring images in very dim light (e.g., night scenes, fluorescence microscopy).
CCD cameras
The tube-type camera has now been largely supplanted by the solid-state chip camera, the
oldest and simplest form of which is the CCD (charge-coupled device). The camera chip con-
tains an array of diodes that function as light buckets. Light entering the semiconductor raises
electrons from the valence to the conduction band, so the number of electrons is a direct lin-
ear measure of the light intensity. The diodes are formed by photolithography, so they have a
Acquiring Images 7
© 2011 by Taylor & Francis Group, LLC
Figure 1.10 The basic principle of CCD operation, illustrated as a set of buckets and conveyors (after
Janesick, 2001).
perfectly regular pattern with no image distortion or sensitivity to the presence of stray fields.
The devices are also inexpensive and rugged compared to tube cameras. CCDs were first
invented and patented at Bell Labs in 1969 (George Smith was awarded the 2009 Nobel Prize
in Physics for this invention), and have now largely displaced film in consumer and profes-
sional still and movie cameras.
The basic operation of a CCD is illustrated in Figure 1.10. Each bucket represents one “pixel”
in the camera (this word has a lot of different meanings in different contexts, as explained
below, so it must be used with some care). With anywhere from a few hundred thousand to
several million detectors on the chip, it is impractical to run wires directly to each one in order
to read out the signal. Instead, the electrons that accumulate in each bucket due to incident
photons are transferred, one line at a time, to a readout row. On a clock signal, each column
of pixels shifts the charge by one location. This places the contents of the buckets into the
readout row, and that row is then shifted, one pixel at a time but much more rapidly, to dump
the electrons into an amplifier, which produces an analog voltage signal that may be sent out
directly or measured to produce the numeric output from a digital camera.
The simplest way of shifting the electrons is shown in Figure 1.11. Every set of three elec-
trodes on the surface of the device constitutes one pixel. By applying a voltage to two of the
electrodes, a field is set up in the semiconductor that acts like a bucket. Electrons are trapped
in the central region by the high fields on either side. Note that this does not reduce the area
sensitive to incoming photons, because electrons generated in the high field regions quickly
migrate to the low field bucket where they are held. By changing the voltage applied to the
regions in six steps or phases, as shown in the figure, the electrons are shifted by one pixel.
First one field region is lowered and the electrons spread into the larger volume. Then the
field on the other side is raised, and the electrons have been shifted by one-third of the pixel
height. Repeating the process acts like a conveyor belt and is the reason for the name “charge-
coupled device.”
Insulator t1 t 2 t3 t4 t5 t6 t1
Semiconductor
1 pixel period
Electron
Wells
Transferring
Charge
Figure 1.11 Varying voltages on a set of three electrodes shifts electrons from one pixel to another in a
CCD.
One significant problem with the chip camera is its spectral response. Even if the chip is
reversed and thinned so that light enters from the side opposite the electrodes, very little blue
light penetrates into the semiconductor to produce electrons. On the other hand, infrared light
penetrates easily and these cameras have red and infrared (IR) sensitivity that far exceeds that
of human vision, usually requiring the installation of a blocking filter to exclude it (because
the IR light is not focused to the same plane as the visible light and thus produces blurred
or fogged images). Figure 1.12 shows this spectral response, which can be further tailored
and extended by using materials other than silicon. The chip can reach a high total efficiency
when antireflective coatings are applied, limited primarily by the “fill factor” — the area frac-
tion of the chip that contains active devices between the narrow ditches that maintain electri-
cal separation. Also, the chip camera has an output that is linearly proportional to the incident
light intensity, convenient for some measurement purposes but very different from human
vision, the vidicon, and photographic film, which are all approximately logarithmic.
Human vision notices brightness differences of a few percent, i.e., a constant ratio of change
rather than a constant increment. Film is characterized by a response to light exposure which
(after chemical development) produces a density vs. exposure curve such as that shown in
Figure 1.13. The low end of this curve represents the fog level of the film, the density that is
present even without exposure. At the high end, the film saturates to a maximum optical den-
sity, for instance based on the maximum physical density of silver particles or dye molecules.
In between, the curve is linear with a slope that represents the contrast of the film. A steep
slope corresponds to a high-contrast film that exhibits a large change in optical density with
a small change in exposure. Conversely, a low-contrast film has a broader latitude to record a
scene with a greater range of brightnesses. The slope of the curve is usually called “gamma.”
Many chip cameras include circuitry or processing that changes their output from linear to
logarithmic so that the image contrast is more familiar to viewers. The more expensive con-
sumer cameras and most professional cameras include the possibility to read the “raw” linear
data as well as the converted image.
Acquiring Images 9
© 2011 by Taylor & Francis Group, LLC
Figure 1.12 Spectral response:
(a) Silicon based chip.
(b) Color sensors in the human eye,
(a)
which are commonly identified as
red, green and blue sensitive but
cover a range of long, medium and
short wavelengths.
(b)
When film is exposed directly to electrons, as in the transmission electron micrograph, rather
than photons (visible light or X-rays), the response curve is linear rather than logarithmic. Many
light photons are needed to completely expose a single silver halide particle for development,
but only a single electron is needed. Consequently, electron image films and plates are often
very high in density (values of optical density greater than 4, which means that 9999/10000 of
incident light is absorbed), which creates difficulties for many scanners and requires more than
8 bits to record.
The trend in camera chips has been to make them smaller and to increase the number of
pixels or diodes present. Some scientific cameras, such as that used in the Hubble telescope,
occupy an entire wafer. But for consumer devices, making each chip one-third, one-quarter,
or even two-tenths of an inch in overall (diagonal) dimension places many devices on a single
wafer and allows greater economic yield. It also requires smaller, less costly lenses. Putting
more pixels into this reduced chip area (for more spatial resolution, as discussed below)
makes the individual detectors small, but the ditches between then have to remain about the
same size to prevent electrons from diffusing laterally. The result is to reduce the total effi-
ciency markedly. Some devices place small lenses over the diodes to capture light that would
Film Density
increase in density (defined as the base-ten
logarithm of the fraction of incident light that
is transmitted) with the logarithm of exposure. Linear Range
(Slope = Gamma)
High (“hard”) contrast corresponds to a steep
curve, while low (“soft”) contrast gives a less
steep curve and films have a greater dynamic
range. Fog
Reciprocity
Level
Failure
Log (Exposure)
otherwise fall into the ditches, but these add cost and also are not so uniform as the diodes
themselves (which are typically within 1% across the entire chip).
The other, and more important, effect of making the detectors small is to reduce their capacity
for electrons, called the well capacity. A typical 15 µm pixel in a scientific grade CCD has a
capacity of about 500,000 electrons, which with low readout noise (as can be achieved in spe-
cial situations) of a few electrons gives a dynamic range greater than photographic film. Even
larger well capacity and dynamic range can be achieved by combining (binning) more detec-
tors for each stored pixel by using more steps in the phase shifting during readout. Reducing
the area of the detector reduces the well size, and with it the dynamic range.
Increasing the noise, for instance by reading out the signal at video rates (each horizontal line
in 52 µs for US standard definition video), dramatically reduces the dynamic range so that a
typical consumer grade video camera has no more than about 64 distinguishable brightness
levels (expensive studio cameras meet the broadcast video specification of 100 levels). Since
with the chip camera these are linear with brightness, they produce even fewer viewable gray
levels, as shown in Figure 1.14. This performance is much inferior to film, which can distin-
guish thousands of brightness levels.
CMOS (Complementary Metal-Oxide Semiconductor) chips can also be used as image sensors,
and in terms of sheer numbers are now more common than the original CCD devices. They
are primarily used in relatively inexpensive consumer cameras and camera phones, although
some have found their way into digital single lens reflex cameras. The conversion of light
photons to electrons functions in the same way as in the CCD chip. The differences start with
the way the signal is read out. In the CMOS designs there are from two to four transistors
immediately adjacent to the light sensor which convert the charge to a voltage and amplify the
signal. In principle, this means that any pixel in the array can be read out directly, addressing
a pixel by row and column just as in a memory chip (Figure 1.15). This is different from the
CCD method of “sweeping” the charge out to one corner of the array, reading all of the pixels
in a fixed order.
The space taken up by these control transistors reduces the “fill factor” or active area of the
chip that is sensitive to light, but this is often compensated for by placing lenses over each
detector to collect light from the dead areas and direct it to the active sensor. The lenses, and
the use of individual amplifiers for each pixel, generally make the sensors in a CMOS detec-
tor less uniform than those in the CCD array, producing a fixed pattern that can be compen-
sated for in software (requiring recording an image with uniform illumination). In addition to
the fixed pattern noise, the CMOS detectors usually have a greater amount of random noise
Acquiring Images 11
© 2011 by Taylor & Francis Group, LLC
Figure 1.14 Comparison of visibility of
gray level steps from linear (equal
steps) and logarithmic (equal ratios)
detectors:
(a) Plots of intensity.
(b) D
isplay of the values from (a).
superimposed on the image signal because of the separate amplifiers, additional wiring and
its associated capacitance and thermal noise, and greater dark current. The very small active
regions (at least in the smaller chips used in pocket cameras and phones, and particularly as
the pixel counts have risen to several million) have small well capacities, resulting in limited
dynamic range for the images. The images are usually stored with 8 bits per channel, because
of the way memory is traditionally organized, but often do not have that much actual bright-
ness resolution.
Larger area CMOS chips are also made which have larger detectors and consequently a greater
well capacity and greater dynamic range. One advantage of the CMOS designs as used in more
expensive cameras arises from the fact that the circuitry to access the pixels can be arranged
along two adjacent sides of the array (addressing the rows and columns, respectively). That
makes it possible to carefully trim away the chip on the other two sides, and arrange four of
the chips together to produce a larger sensor with higher pixel counts. This approach, com-
bined with the use of much larger sensors to achieve greater sensitivity and dynamic range,
has led some manufacturers to prefer CMOS detectors as large as a traditional film negative
for digital single-lens reflex cameras.
The advantages of CMOS sensors lie primarily in three areas: they consume much less power,
and so give better battery life; the amplifier and digitization circuitry can be placed on the
same chip to reduce size and perhaps increase ruggedness; and the production methods for
the wafers are essentially the same as those used for other standard silicon devices such as
memory and processors, whereas CCD wafers require unique processing. The latter advantage
is somewhat offset by the fact that high quality CMOS sensors do require somewhat custom-
ized fabrication for greater uniformity and fewer noise-producing imperfections than can be
tolerated in other devices. While the cost to fabricate CMOS sensors is less than for CCD, the
design costs are much higher. Of course, for devices that are to be produced in large quantity,
this is a minor factor. The overall trend has been for CMOS sensors to continue to improve
in quality and performance, and while the advantages of the CCD sensor are still important
for most technical applications, it is wise to consider the trade-offs on a case-by-case basis
(Nakamura, 2006; Holst & Lomheim, 2007).
Acquiring Images 13
© 2011 by Taylor & Francis Group, LLC
Figure 1.16
(a) Interlace scan covers even
Line
1
numbered lines in one six-
2 tieth-second field, and even
3
4 numbered lines in a second
5
6 field.
7
8
9
(b) W hen motion is present
10 (either in the scene or caused
11
12 by camera motion), this pro-
13
14 duces an offset in the com-
Field 1 Field 2 plete image.
(a)
(b)
vertical resolution but for casual viewing purposes is not noticeable. Motion can cause the
even and odd fields of a full frame to be offset from each other, producing a significant deg-
radation of the image, as shown in the figure. A similar effect occurs with stationary images
if the horizontal retrace signal is imprecise or difficult for the electronics to lock onto; this is
a particular problem with signals played back from consumer video tape recorders. (Moving
images are also distorted with progressive scan cameras, due to the time required to read from
the top of the image to the bottom.)
During the transfer and readout process, unless the camera is shuttered either mechanically
or electrically, photons continue to produce electrons in the chip. This produces a large
Color cameras
Color cameras can be designed in three principal ways, as shown in Figures 1.17, 1.18,
and 1.19. For stationary images (which includes many scientific applications such as
microscopy, but excludes “real-time” applications such as video), a single detector array
can be used to acquire three sequential exposures through red, green and blue filters,
respectively (Figure 1.17), which are then combined for viewing. The advantages of this
scheme include low cost and the ability to use different exposure times for the different
color bands, which can compensate for the poorer sensitivity of the silicon chip to short
wavelength (blue) light.
Many high-end consumer and most professional grade video cameras use three sensors
(Figure 1.18). A prism array splits the incoming light into red, green, and blue components,
which are recorded by three different sensors whose outputs are combined electronically to
produce a standard video image. This approach is more costly, since three chips are needed,
but for video applications they need not be of particularly high resolution (even a high-defini-
tion video camera has many fewer pixels than a digital still camera). The optics and hardware
to keep everything in alignment add some cost, and the depth of the prism optics makes it
Acquiring Images 15
© 2011 by Taylor & Francis Group, LLC
Figure 1.17 Schematic diagram of a color
wheel camera with red, green and blue
filters. The fourth filter position is empty,
allowing the camera to be used as a mono-
chrome detector with greater sensitivity for
dim images (e.g., fluorescence microscopy).
impractical to use short focal length (wide angle) lenses. This design is rarely used in digital
still cameras.
Video images are often digitized into a 640 × 480 array of stored pixels (the dimensions of
the VGA display that was once standard for personal computers), but this is not the actual
resolution of the image. The broadcast bandwidth limits the high frequencies and eliminates
any rapid variations in brightness and color. A standard definition video image has no more
than 330 actual elements of resolution in the horizontal direction for the brightness (lumi-
nance) signal, and about half that for the color (chrominance) information. Color information
is intentionally reduced in resolution because human vision is not very sensitive to blurring
of color beyond boundary lines.
Of course, video signals can be further degraded by poor equipment. Recording video on
consumer-grade tape machines can reduce the resolution by another 50% or more, particularly
if the record head is dirty or the tape has been used many times before (an unfortunately very
(a) (b)
common problem with forensic examination of surveillance video is that the tapes are played
— over and over — for visual examination by local police so that by the time professionals get
them, the oxide coating — and the information — has been damaged or even removed). Video
images are not very high resolution, although HDTV (high definition television) has improved
things somewhat. Consequently, video technology is usually a poor choice for scientific imag-
ing unless there is some special need to capture “real time” images (i.e., 25–30 frames per
second) to record changes or motion. Digital still cameras have largely replaced them, as they
produce much higher resolution images with greater dynamic range.
Most digital cameras use a single pixel array, often of very high pixel (detector) count, with a
color filter that allows red, green, and blue light to reach specific detectors. Different patterns
may be used (Figure 1.19), with the Bayer pattern being very common (invented by Kodak
researcher Bryce Bayer and the basis for U.S. Patent 3,971,065 “Color Imaging Array,” issued in
1976). Notice that it assigns twice as many detectors for green as for red or blue, which mim-
ics to some extent the human eye’s greater sensitivity to green. The problem with the single-
chip camera, of course, is that the image resolution in each color channel is reduced. The red
intensity at some locations must be interpolated from nearby sensors, for example. It is also
necessary to design the filters to give the same brightness sensitivity in each channel. If this is
not done well, a herring-bone pattern (often referred to as a “zipper”) appears in images of a
uniform gray test card and color fringes appear along contrast edges in the picture, as shown
in Figure 1.20.
Interpolation techniques for Bayer pattern color filters reduce the image resolution as com-
pared to the number of individual detectors in the camera (which is generally the speci-
fication advertised by the manufacturer). Inherently, this “demosaicking” process involves
trade-offs between image sharpness, details, noise, processing time and conversion artifacts.
The quality of the result, judged by its ability to preserve sharp boundaries in brightness
while minimizing the introduction of color artifacts, varies inversely with the computational
Acquiring Images 17
© 2011 by Taylor & Francis Group, LLC
Figure 1.20 Example of “zipper” patterns resulting from poor interpolation in a single-chip digital
camera.
Camera resolution
The signal coming from the silicon detector is analog, even if the digitization takes place within
the camera housing or even on the same chip, so the interpolation is done in the amplifier
stage. In most cases, the actual image resolution with a single chip camera and filter arrange-
ment is one-half to two-thirds the value that might be expected from the advertised number
of pixels in the camera, because of this interpolation. And some cameras record images with
many more stored pixels than the chip resolution warrants in any case. Such interpolation and
empty magnification contribute no additional information in the image.
Acquiring Images 19
© 2011 by Taylor & Francis Group, LLC
readout noise. Digital still cameras read out the data in one single pass (progressive scan), not
with an interlace. By cooling the chip and amplifier circuitry to reduce dark currents, integra-
tion (long exposures up to tens of seconds, or for some astronomical applications many min-
utes) can be used to advantage because of the high dynamic range (large well size and large
number of bits in the digitizer) of some chip designs. In addition, the ability to use a physical
rather than electronic shutter simplifies chip circuitry and increases fill factor. The number of
pixels in video cameras need not be any greater than the resolution of the video signal, which,
as noted above, is rather poor. In a digital still camera, very high pixel counts can give rise to
extremely high resolution, which rivals film in some cases.
There is also an interesting cross-over occurring between high end consumer and professional
scientific grade cameras. In addition to dedicated cameras for attachment to microscopes or
other separate optics, manufacturers are producing consumer single-lens reflex cameras with
enough resolution (15 to 20 million pixels at this writing) that it is becoming practical to use
them in technical applications, and simple optical attachments make it easy to connect them
to microscopes or other instruments (and of course the camera may also be removed and used
for other purposes). The camera may be tethered directly to a computer, but in many cases it
is more practical to record the images to memory chips that are later downloaded to the com-
puter. Professional digital cameras with large, high resolution detector arrays, interchangeable
lenses, etc., are providing capabilities that compete with traditional 35mm and larger film
cameras. Every manufacturer of cameras has recognized the shift away from film and toward
digital recording, and an incredibly wide variety of cameras is now available, with new devel-
opments appearing frequently.
The benefits of a camera with a large number of sensors (high pixel count), as well as large
individual sensors (large well size and consequent high dynamic range), seem obvious and
desirable. For some applications, high pixel counts are not so important. At high optical
magnification, the important limitation is the optical resolution. In the rather typical setup
of my bench microscope, with a 10x (low magnification) objective lens, the image projected
onto the chip by the transfer optics covers about 1600 µm width on the specimen. With a
100× (high magnification) objective lens that becomes 160 µm. For a camera with 3600 ×
2400 sensors (less than 10 megapixels) the low magnification image is recorded at about 1
pixel per micron, adequate for the resolution of the optics. The high magnification image
is recorded with 90 pixels per micron. Since the optical resolution of the microscope under
optimum conditions is about 0.5 µm with the 100× lens, this produces a vast and unneces-
sary oversampling. At low magnifications, or for viewing fine detail in large scenes (such as
aerial and satellite imagery), high pixel counts make sense. When the limitation on resolu-
tion lies with the optics, it may not.
Focusing
Regardless of what type of camera is employed to acquire images, it is important to focus the
optics correctly to capture the fine details in the image. Often the human eye is used to per-
form this task manually. In some situations, such as automated microscopy of pathology slides
or surveillance tracking of vehicles, automatic focusing is required. This brings computer
processing into the initial step of image capture. Sometimes, in the interests of speed, the
processing is performed in dedicated hardware circuits attached to the camera. But in many
cases the algorithms are the same as might be applied in the computer (described in Chapter
Acquiring Images 21
© 2011 by Taylor & Francis Group, LLC
scientific imaging systems such as digital cameras, direct-scan microscopes (the scanning
electron microscope or SEM, scanning tunneling microscope or STM, the atomic force
microscope or AFM, etc.), flat-bed scanners, film or slide digitizers, and similar devices use
progressive scan.
HDTV modes include many more differences from conventional television than the use
of progressive scan. The pixel density is much higher, with a wider aspect ratio of 16:9
(instead of the 4:3 used in NTSC television) and the pixels are square. A typical HDTV
mode presents 1920 × 1080 pixel images at the rate of 60 full scans per second, for a total
data rate exceeding 2 gigabits per second, several hundred times as much data as analog
broadcast television. One consequence of this high data rate is the use of data compression
techniques, which are discussed in Chapter 3, and the use of digital transmission tech-
niques using cable or optical fiber instead of broadcast channels. Over-the-air, satellite,
and cable transmission of HDTV signals all involve compression, often with a significant
loss of image quality.
Regardless of the effects on consumer television, the development of HDTV hardware is likely
to produce spin-off effects for computer imaging, such as high pixel density cameras with pro-
gressive scan output, high bandwidth recording devices, and superior CRT or LCD displays.
For example, color cameras being designed for HDTV applications output digital rather than
analog information by performing the analog-to-digital conversion within the camera, with at
least 10 bits each for red, green, and blue.
Even the best system can be degraded in performance by such simple things as cables, con-
nectors, or incorrect termination impedance. Another practical caution in the use of standard
cameras is to avoid automatic gain or brightness compensation circuits. These can change the
image contrast or linearity in response to bright or dark regions that do not even lie within
the digitized portion of the image, make comparison between images difficult, and increase
the gain and noise for a dim signal.
Figure 1.21 shows a micrograph with its brightness histogram. This is an important tool for
image analysis, which plots the number of pixels as a function of their brightness values. It
is used extensively in subsequent chapters. The histogram shown is well spread out over the
available 256 brightness levels, with peaks corresponding to each of the structures in the
metal sample. If a bright light falls on a portion of the detector in the solid-state camera that is
Figure 1.21 A gray scale image digitized from a metallographic microscope and its brightness histo-
gram, which plots the number of pixels with each possible brightness value.
Figure 1.22 An image taken with filtered sunlight and an effective color temperature of 5000K, but
stored as a raw file and opened using different assumed color temperatures. From left to right, 3500,
4500, 5500, 6500K.
Acquiring Images 23
© 2011 by Taylor & Francis Group, LLC
Figure 1.23 Digitization of an analog
voltage signal along one line in an
image (blue) produces a series of
values that correspond to a series
of steps (red) equal in time and
rounded to integral multiples of the
smallest measurable increment.
Pixels
It is most desirable to have the spacing of the pixel values be the same in the horizontal and
vertical directions (i.e., square pixels), as this simplifies many processing and measurement
operations. There are some theoretical advantages to having pixels arranged as a hexagonal
grid, but because of the way that all acquisition hardware actually functions, and to simplify
the addressing of pixels in computer memory, this is almost never done.
Accomplishing the goal of square pixels with an analog video camera requires a well-adjusted
clock to control the acquisition. Since the standard-definition video image is not square, but
has a width-to-height ratio of 4:3, the digitized image may represent only a portion of the
entire field of view. Digitizing boards (frame grabbers) were first designed to record 512 × 512
arrays of values, since the power-of-two dimension simplified design and memory addressing.
Later generations acquired a 640 wide by 480 high array, which matched the image propor-
tions and the size of standard VGA display monitors while keeping the pixels square. Because
of the variation in clocks between cameras and digitizers, it was common to find distortions of
several percent in pixel squareness. This can be measured and compensated for after acquisi-
tion by resampling the pixels in the image, as Chapter 4 describes. Most digital still cameras
acquire images that have a width-to-height ratio of 4:3 (the aspect ratio of conventional video)
or 3:2 (the aspect ratio of 35mm film) and have square pixels.
Since pixels have a finite area, those which straddle a boundary in the scene effectively aver-
age the brightness levels of two regions and have an intermediate brightness that depends on
how the pixels lie with respect to the boundary. This means that a high lateral pixel resolution
and a large number of distinguishable gray levels are needed to accurately locate boundaries.
Figure 1.24 shows several examples of an image with varying numbers of pixels across its
width, and Figure 1.25 shows the same image with varying numbers of gray levels.
For the most common types of image acquisition devices, such as cameras, the pixels rep-
resent an averaging of the signal across a finite area of the scene or specimen. However,
there are other situations in which this is not so. At low magnification, for example, the
scanning electron microscope beam samples a volume of the specimen much smaller
than the dimension of a pixel in the image. So does the probe tip in a scanned probe
microscope. Range imaging of the moon from the Clementine orbiter determined the
elevation of points about 10 cm in diameter using a laser rangefinder, but at points spaced
apart by 100 meters or more.
In these cases, the interpretation of the relationship between adjacent pixels is slightly dif-
ferent. Instead of averaging across boundaries, the pixels sample points that are discrete and
well separated. Cases of intermediate or gradually varying values from pixel to pixel are rare,
and the problem instead becomes how to locate a boundary between two sampled points on
either side. If there are many points along both sides of the boundary, and the boundary can
be assumed to have some geometric shape (such as a locally straight line), fitting methods can
Acquiring Images 25
© 2011 by Taylor & Francis Group, LLC
Figure 1.25 Four representations of the same image, with variation in the number of gray levels used.
From the upper left: 32; 16; 8; 4. In all cases, a full 256 × 256 array of pixels is retained. Each step in
the coarsening of the image is accomplished by rounding the brightness of the original pixel value.
be used to locate it to a fraction of the pixel spacing. These methods are discussed further in
Chapter 10 on image measurements.
Acquiring Images 27
© 2011 by Taylor & Francis Group, LLC
Figure 1.26 The same image recorded with an ASA setting of 1600 (top) and 100 (bottom), showing the
increase in random pixel noise produced by higher gain in the camera.
images were recorded with the same camera and identical illumination and aperture settings;
changing the ASA setting on the camera resulted in different exposure times.
Figure 1.27 shows a fluorescence microscope image in which a single video frame illustrates
substantial statistical noise, which prevents distinguishing or measuring the structures pres-
ent. Averaging together multiple frames collects more signal and results in an improvement in
the signal- to noise-ratio, and hence in the visibility of detail.
Noise
Images in which the pixel values vary within regions that are ideally uniform in the original scene
can arise either because of limited counting statistics for the photons or other signals, losses intro-
duced in the shifting of electrons within the chip, or due to electronic noise in the amplifiers or
cabling. In any case, the variation is generally referred to as noise, and the ratio of the contrast
which is due to differences present in the scene represented by the image to the noise level is
the signal-to-noise ratio. When this is low, the features present may be invisible to the observer.
Figure 1.28 shows an example in which several features of different size and shape are super-
imposed on a noisy background with different signal-to-noise ratios. The ability to discern the
presence of the features is generally proportional to their area and independent of shape.
In the figure, a smoothing operation is performed on the image with the poorest signal-to-
noise ratio, which somewhat improves the visibility of the features. The methods available
for improving noisy images by image processing are discussed in the chapters on spatial and
frequency domain methods. However, the best approach to noisy images, when it is available,
is to collect more signal and improve the statistics.
Increasing the exposure (either by increasing the exposure time, the lens aperture, or the
illumination) reduces noise due to statistical effects, as shown in Figures 1.26 and 1.27. The
improvement in quality is proportional to the square root of the amount of light (or other
signal) collected. It is necessary to use a detector with a sufficient well size to hold the elec-
trons and to use a sufficiently high bit depth in the image to preserve the contrast details.
Cooling the detector and associated electronics chips can reduce electronic noise during long
acquisitions of many minutes. Most uncooled camera chips begin to show unacceptable pixel
noise due to dark current with integration times of more than a few seconds.
Acquiring images at video rates of 25–30 frames per second is sometimes referred to as “real
time” imaging, but of course this term should properly be reserved for any imaging rate that
is adequate to reveal temporal changes in a particular application. For some situations, time-
lapse photography may only require one frame to be taken at periods of many minutes, hours,
or even days. For others, very short exposures and high rates are needed. Special cameras
that do not use video frame rates or bandwidths can achieve rates up to ten times that of a
standard video camera for full frames and even higher for small image dimensions. These
cameras typically use a single line of detectors and optical deflection (e.g., a rotating mirror
or prism) to cover the image area.
For many applications, the repetition rate does not need to be that high. Either stroboscopic
imaging or a fast shutter speed may be enough to stop the important motion to provide a
sharp image. Electronic shutters can be used to control solid state imaging devices, instead
Acquiring Images 29
© 2011 by Taylor & Francis Group, LLC
Figure 1.28 Features on a
noisy background:
(a) signal-to-noise ratio 1:1;
(a) (b)
(b) signal-to-noise ratio 1:3;
(c) s ignal-to-noise ratio 1:7;
(d) i mage (c) after spatial
smoothing.
(c) (d)
of a mechanical shutter. Exposure times under 1/10,000th of a second can easily be achieved,
but of course this short exposure requires high illumination intensity.
Acquiring Images 31
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"Men who, 'mid noise and dirt, and play and prate,
Could calmly mend the pen, and wash the slate."
and which are not without their charm even to him "who whistles as
he goes for want of thought." No wonder, then, if Yverdun seemed
Paradisaical in its landscapes. Nor was this all. If the views outside
were charming, our domestic and social relations within doors were
not less pleasing. At first, the unwelcome vision of the late head-
master would sometimes haunt us, clad in his flowing black D.D.
robes—"tristis severitas in vultu, atque in verbis fides," looking as if
he intended to flog, and his words never belying his looks. That
terrible Olympian arm, raised and ready to strike, was again
shadowed forth to view; while we could almost fancy ourselves once
more at that judicial table, one of twenty boys who were to draw
lots for a "hander." How soothingly, then, came the pleasing
consciousness, breaking our reverie, that a very different person was
now our head-master—a most indulgent old man whom we should
meet ere long, with hands uplifted, indeed, but only for the purpose
of clutching us tight while he inflicted a salute on both cheeks, and
pronounced his affectionate guten morgen, liebes kind, as he
hastened on to bestow the like fatherly greeting upon every pupil in
turn.
THE DORMITORY.
The sleeping apartments at the chateau occupied three of the four
sides of its inner quadrangle, and consisted of as many long rooms,
each with a double row of windows; whereof one looked into the
aforesaid quadrangle, while the opposite rows commanded,
severally, views of the garden, the open country, and the Grande
Place of the town. They were accommodated with sixty uncurtained
stump bedsteads, fifty-nine of which afforded gîte to a like number
of boys; and one, in no respect superior to the rest, was destined to
receive the athletic form of Herr Gottlieb, son-in-law to Vater
Pestalozzi, to whose particular charge we were consigned during the
hours of the night. These bedrooms, being as lofty as they were
long, broad, and over-furnished with windows, were always
ventilated; but the in-draught of air, which was sufficient to keep
them cool during the hottest day in summer, rendered them cold,
and sometimes very cold, in the winter. In that season, accordingly,
especially when the bise blew, and hail and sleet were pattering
against the casements, the compulsory rising to class by candlelight
was an ungenial and unwelcome process; for which, however, there
being no remedy, the next best thing was to take it as coolly, we
were going to say—that of course—but, as patiently as might be.
The disagreeable anticipation of the réveil was frequently enough to
scare away sleep from our eyes a full hour before the command to
jump out of bed was actually issued. On such occasions we would lie
awake, and, as the time approached, begin to draw in our own
breath, furtively listening, not without trepidation, to the loud nose
of a distant comrade, lest its fitful stertor should startle another pair
of nostrils, on whose repose that of the whole dormitory depended.
Let Æolus and his crew make what tumult they liked inside or
outside the castle—they disturbed nobody's dreams—they never
murdered sleep. Let them pipe and whistle through every keyhole
and crevice of the vast enceinte of the building—sigh and moan as
they would in their various imprisonments of attic or corridor; howl
wildly round the great tower, or even threaten a forcible entry at the
windows, nobody's ears were scared into unwelcome consciousness
by sounds so familiar to them all. It was the expectation of a blast
louder even than theirs that would keep our eyes open—a blast
about to issue from the bed of Herr Gottlieb, and thundering
enough, when it issued, to startle the very god of winds himself!
Often, as the dreaded six A.M. drew nigh, when the third quarter
past five had, ten minutes since, come with a sough and a rattle
against the casements, and still Gottlieb slept on, we would take
courage, and begin to dream with our eyes open, that his slumbers
might be prolonged a little; his face, turned upwards, looked so
calm, the eyes so resolutely closed—every feature so perfectly at
rest. It could not be more than five minutes to six—might not he
who had slept so long, for once oversleep himself? Never! However
placid those slumbers might be, they invariably forsook our
"unwearied one" just as the clock was on the point of striking six. To
judge by the rapid twitchings—they almost seemed galvanic—first of
the muscles round the mouth, then of the nose and eyes, it
appeared as though some ill-omened dream, at that very nick of
time, was sent periodically, on purpose to awaken him; and, if so, it
certainly never returned απρακτος. Gottlieb would instantly set to
rubbing his eyes, and as the hour struck, spring up wide awake in
his shirt sleeves—thus destroying every lingering, and, as it always
turned out, ill-founded hope of a longer snooze. Presently we beheld
him jump into his small-clothes, and, when sufficiently attired to be
seen, unlimber his tongue, and pour forth a rattling broadside—Auf,
kinder! schwind!—with such precision of delivery, too, that few
sleepers could turn a deaf ear to it. But, lest any one should still lurk
under his warm coverlet out of earshot, at the further end of the
room, another and a shriller summons to the same effect once more
shakes the walls and windows of the dormitory. Then every boy
knew right well that the last moment for repose was past, and that
he must at once turn out shivering from his bed, and dress as fast as
possible; and it was really surprising to witness how rapidly all could
huddle on their clothes under certain conditions of the atmosphere!
In less than five minutes the whole school was dressed, and
Gottlieb, in his sounding shoes, having urged the dilatory with
another admonitory schwind, schwind! has departed, key and candle
in hand, to arouse the remaining sleepers, by ringing the "Great
Tom" of the chateau. So cold and cheerless was this matutinal
summons, that occasional attempts were made to evade it by
simulated headach, or, without being quite so specific, on the plea of
general indisposition, though it was well known beforehand what the
result would be. Herr Gottlieb, in such a case, would presently
appear at the bedside of the delinquent patient, with very little
compassion in his countenance, and, in a business tone, proceed to
inquire from him, Why not up?—and on receiving for reply, in a
melancholy voice, that the would-be invalid was sehr krank, would
instantly pass the word for the doctor to be summoned. That doctor
—we knew him well, and every truant knew—was a quondam French
army surgeon—a sworn disciple of the Broussais school, whose
heroic remedies at the chateau resolved themselves into one of two
—i. e., a starve or a vomit, alternately administered, according as
the idiosyncracy of the patient, or as this or that symptom turned
the scale, now in favour of storming the stomach, now of starving it
into capitulation. Just as the welcome hot mess of bread and milk
was about to be served to the rest, this dapper little Sangrado would
make his appearance, feel the pulse, inspect the tongue, ask a few
questions, and finding, generally, indications of what he would term
une légère gastrite, recommend diète absolue; then prescribing a
mawkish tisane, composed of any garden herbs at hand, and
pocketing lancets and stethoscope, would leave the patient to
recover sans calomel—a mode of treatment to which, he would tell
us, we should certainly have been subjected in our own country.
Meanwhile, the superiority of his plan of treatment was
unquestionable. On the very next morning, when he called to visit
his cher petit malade, an empty bed said quite plainly, "Very well, I
thank you, sir, and in class." But these feignings were comparatively
of rare occurrence; in general, all rose, dressed, and descended
together, just as the alarum-bell had ceased to sound; and in less
than two minutes more all were assembled in their respective class-
rooms. The rats and mice, which had had the run of these during
the night, would be still in occupation when we entered; and such
was the audacity of these vermin that none cared alone to be the
first to plant a candle on his desk. But, by entering en masse, we
easily routed the Rodentia, whose forces were driven to seek shelter
behind the wainscot, where they would scuffle, and gnaw, and
scratch, before they finally withdrew, and left us with blue fingers
and chattering teeth to study to make the best of it. Uncomfortable
enough was the effort for the first ten minutes of the session; but by
degrees the hopes of a possible warming of hands upon the surface
of the Dutch stoves after class, if they should have been lighted in
time, and at any rate the certainty of a hot breakfast, were
entertained, and brought their consolation; besides which, the being
up in time to welcome in the dawn of the dullest day, while health
and liberty are ours, is a pleasure in itself. There was no exception to
it here; for when the darkness, becoming every moment less and
less dark, had at length given way, and melted into a gray gloaming,
we would rejoice, even before it appeared, at the approach of a new
day. That approach was soon further heralded by the fitful notes of
small day-birds chirping under the leaves, and anon by their sudden
dashings against the windows, in the direction of the lights not yet
extinguished in the class-rooms. Presently the pigs were heard
rejoicing and contending over their fresh wash; then the old horse
and the shaggy little donkey in the stable adjoining the styes,
knowing by this stir that their feed was coming, snorted and brayed
at the pleasant prospect. The cocks had by this time roused their
sleepy sultanas, who came creeping from under the barn-door to
meet their lords on the dunghill. Our peacock, to satisfy himself that
he had not taken cold during the night, would scream to the utmost
pitch of a most discordant voice; then the prescient goats would
bleat from the cabins, and plaintively remind us that, till their door is
unpadlocked, they can get no prog; then the punctual magpie, and
his friend the jay, having hopped all down the corridor, would be
heard screaming for broken victuals at the school-room door, till our
dismissal bell, finding so many other tongues loosened, at length
wags its own, and then for the next hour and a half all are free to
follow their own devices. Breakfast shortly follows; but, alas! another
cold ceremony must be undergone first. A preliminary visit to pump
court, and a thorough ablution of face and hands, is indispensable to
those who would become successful candidates for that long-
anticipated meal. This bleaching process, at an icy temperature, was
never agreeable; but when the pipes happened to be frozen—a
contingency by no means unfrequent—and the snow in the yard
must be substituted for the water which was not in the pump, it
proved a difficult and sometimes a painful business; especially as
there was always some uncertainty afterwards, whether the
chilblained paws would pass muster before the inspector-general
commissioned to examine them—who, utterly reckless as to how the
boys might "be off for soap," and incredulous of what they would
fain attribute to the adust complexion of their skin, would require to
have that assertion tested by a further experiment at the "pump
head."
THE REFECTORY.
"Forbear to scoff at woes you cannot feel,
Nor mock the misery of a stinted meal."—Crabbe.
The dietary tables at the chateau, conspicuous alike for the paucity
and simplicity of the articles registered therein, are easily recalled to
mind. The fare they exhibited was certainly coarse—though, by a
euphemism, it might have been termed merely plain—and spare
withal. The breakfast would consist of milk and water—the first
aqueous enough without dilution, being the produce of certain ill-
favoured, and, as we afterwards tasted their flesh, we may add ill-
flavoured kine, whose impoverished lacteals could furnish out of
their sorry fodder no better supplies. It was London sky-blue, in
short, but not of the Alderney dairy, which was made to serve our
turn at Yverdun. This milk, at seven in summer, and at half-past
seven in winter, was transferred boiling, and as yet unadulterated,
into earthenware mixers, which had been previously half-filled with
hot water from a neighbouring kettle. In this half-and-half state it
was baled out for the assembled school into a series of pewter
platters, ranged along the sides of three bare deal boards, some
thirty feet long by two wide, and mounted on tressels, which served
us for tables. The ministering damsels were two great German
Fraus, rejoicing severally in the pleasing names of Gretchen and
Bessie. When Frau Gretchen, standing behind each boy, had dropt
her allowance of milk over his right shoulder—during which process
there was generally a mighty clatter for full measure and fair play—
the other Frau was slicing off her slices of bread from a brown loaf a
yard long, which she carried under her arm, and slashed clean
through with wonderful precision and address. It was now for all
those who had saved pocket-money for menus-plaisirs to produce
their cornets of cinnamon or sugar, sprinkle a little into the milk, and
then fall to sipping and munching with increased zest and
satisfaction. So dry and chaffy was our pain de ménage that none
ventured to soak it entire, or at once, but would cut it into
frustrums, and retain liquid enough to wash down the boluses
separately. In a few minutes every plate was completely cleaned out
and polished; and the cats, that generally entered the room as we
left it, seldom found a drop with which they might moisten their
tongues, or remove from cheeks and whiskers the red stains of
murdered mice on which they had been breaking their fast in the
great tower. So much for the earliest meal of the day, which was to
carry us through five hours, if not of laborious mental study, at least
of the incarceration of our bodies in class, which was equally
irksome to them as if our minds had been hard at work. These five
hours terminated, slates were once more insalivated and put by
clean, and the hungry garrison began to look forward to the
pleasures of the noon-day repast. The same bell that had been
calling so often to class would now give premonitory notice of
dinner, but in a greatly changed tone. In place of the shrill snappish
key in which it had all the morning jerked out each short unwelcome
summons from lesson to lesson, as if fearful of ringing one note
beyond the prescribed minute, it now would take time, vibrate far
and wide in its cage, give full scope to its tongue, and appear, from
the loud increasing swell of its prolonged oyez, to announce the
message of good cheer like a herald conscious and proud of his
commission. Ding-dong!—come along! Dinner's dishing!—ding-dong!
Da capo and encore! Then, starting up from every school-room form
throughout the chateau, the noisy boys rushed pell-mell, opened all
the doors, and, like emergent bees in quest of honey, began
coursing up and down right busily between the salle-à-manger and
the kitchen—snuffing the various aromas as they escaped from the
latter into the passage, and inferring from the amount of exhaled
fragrance the actual progress of the preparations for eating.
Occasionally some "sly Tom" would peep into the kitchen, while the
Fraus were too busy to notice him, and watch the great cauldron
that had been milked dry of its stores in the morning, now
discharging its aqueous contents of a much-attenuated bouillon—the
surface covered with lumps of swimming bread, thickened
throughout with a hydrate of potatoes, and coloured with coarse
insipid carrots, which certainly gave it a savoury appearance. It was
not good broth—far from it, for it was both sub-greasy and super-
salted; but then it was hot, it was thick, and there was an abundant
supply. It used to gush, as we have said, from the great stop-cock of
the cauldron, steaming and sputtering, into eight enormous tureens.
The shreds of beef, together with whatever other solids remained
behind after the fluid had been drawn off, were next fished up from
the abyss with long ladles, and plumped into the decanted liquor.
The young gastronome who might have beheld these proceedings
would wait till the lid was taken off the sauerkraut; and then, the
odour becoming overpoweringly appetising, he would run, as by
irresistible instinct, into the dining-room, where most of the boys
were already assembled, each with a ration of brown bread in his
hand, and ready for the Fraus, who were speedily about to enter.
The dinner was noisy and ungenteel in the extreme—how could it be
otherwise? ventre affamé n'a point d'oreilles. Hardly was the German
grace concluded, and the covers removed, when that bone of
contention, the marrow bone, was caught up by some big boy near
the top of the table, and became the signal for a general row. All in
his neighbourhood would call out second, third, fourth, fifth, &c., for
said bone; and thus it would travel from plate to plate, yielding its
contents freely to the two or three first applicants, but wholly
inadequate—unless it could have resolved itself altogether into
marrow—to meet all the demands made upon its stores. Then arose
angry words of contention, which waxed hot as the marrow waxed
cold, every candidate being equally vociferous in maintaining the
priority of his particular claim. Earnest appeals in German, French,
Spanish, English, &c., were bandied from one to the other in
consequence, as to who had really said après toi first! At last the
"dry bone" was found undeserving of further contention; and,
ceasing to drop any more fatness upon any boy's bread, the
competition for it was dropt too. When now we had half-filled our
stomachs with a soup which few physicians would have withheld
from their fever patients on the score of its strength, we threw in a
sufficiency of bread and sauerkraut to absorb it; and, after the post-
prandial German grace had been pronounced, the boys left the
table, generally with a saved crust in their pockets, to repair to the
garden and filch—if it was filching—an alliaceous dessert from the
beds, which they washed in the clear stream, and added, without
fear of indigestion, to the meal just concluded within the chateau.
Most of us throve upon this Spartan diet; but some delicate boys,
unendowed with the ostrich power of assimilation usual at that
period—for boys, like ostriches, can digest almost anything—became
deranged in their chylopoietics, and continued to feel its ill effects in
mesenteric and other chronic ailments for years afterwards. An hour
was given for stomachs to do their work, before we reassembled to
ours in the class-room. At half-past four precisely, a gouté, was
served out, which consisted of a whacking slice of bread, and either
a repetition of the morning's milk and water, or café au lait, (without
sugar "bien entendu,") or twenty-five walnuts, or a couple of ounces
of strong-tasted gruyère, or a plateful of schnitz (cuttings of dried
apples, pears, and plums). We might choose any one of these
several dainties we liked, but not more. Some dangerous characters
—not to be imitated—would occasionally, while young Frau Schmidt
stood doling out the supplies from her cupboard among the
assembled throng, make the disingenuous attempt to obtain cheese
with one hand and schnitz with the other. But the artifice, we are
happy to say, seldom succeeded; for that vigilant lady, quick-eyed
and active, and who, of all things, hated to be imposed upon, would
turn round upon the false claimant, and bid him hold up both his
hands at once—which he, ambidexter as he was, durst not do, and
thus he was exposed to the laughter and jeers of the rest. At nine,
the bell sounded a feeble call to a soi-disant supper; but few of us
cared for a basin of tisane under the name of lentil soup—or a pappy
potato, salted in the boiling—and soon after we all repaired to our
bedrooms—made a noise for a short time, then undressed, and were
speedily asleep under our duvets, and as sound, if not as musical, as
tops.
Our common fare, as the reader has now seen, was sorry enough;
but we had our Carnival and gala days as well as our Lent. Vater
Pestalozzi's birthday, in summer, and the first day of the new year,
were the most conspicuous. On each of these occasions we enjoyed
a whole week's holiday; and as these were also the periods for
slaughtering the pigs, we fed (twice a-year for a whole week!) upon
black puddings and pork à discretion, qualified with a sauce of
beetroot and vinegar, and washed down with a fluid really like small-
beer.
CLASSES.
The school-rooms, which lay immediately under the dormitories on
the ground-floor, consisted of a number of detached chambers, each
of which issued upon a corridor. They were airy—there was plenty of
air at Yverdun—and lofty as became so venerable a building; but
they were unswept, unscrubbed, peeled of their paint, and, owing to
the little light that could find its way through two very small windows
punched out of the fortress walls, presented, save at mid-day, or as
the declining sun illumined momentarily the dark recess, as
comfortless a set of interiors as you could well see. It required,
indeed, all the elasticity of youth to bear many hours' daily
incarceration in such black-holes, without participating in the
pervading gloom. Such dismal domiciles were only fit resorts for the
myoptic bat, who would occasionally visit them from the old tower;
for the twilight horde of cockroaches, which swarmed along the
floor, or the eight-eyed spiders who colonised the ceiling. The tender
sight, too, of a patient just recovering from ophthalmia would here
have required no factitious or deeper shade—but merits like these
only rendered them as ungenial as possible to the physiology and
feelings of their youthful occupants. If these apartments looked
gloomy in their dilapidations and want of sun, the sombre effect was
much heightened by the absence of the ordinary tables and chairs,
and whatever else is necessary to give a room a habitable
appearance. Had an appraiser been commissioned to make out a
complete list of the furniture and the fixtures together, a mere
glance had sufficed for the inventory. In vain would his practised eye
have wandered in quest of themes for golden sentences, printed in
such uncial characters that all who run may read; in vain for the
high-hung well-backed chart, or for any pleasing pictorial souvenirs
of Æsop or the Ark—neither these nor the long "coloured Stream of
Time," nor formal but useful views in perspective, adorned our sorry
walls. No old mahogany case clicked in a corner, beating time for the
class, and the hour up-striking loud that it should not be defrauded
of its dues. No glazed globe, gliding round on easy axis, spun under
its brassy equator to the antipodes on its sides being touched. No
bright zodiac was there to exhibit its cabalistic figures in pleasing
arabesques. In place of these and other well-known objects, here
stood a line of dirty, much-inked desks, with an equally dirty row of
attendant forms subjacent alongside. There was a scantling—it
seldom exceeded a leash—of rickety rush-bottom chairs distributed
at long intervals along the walls; a coal-black slate, pegged high on
its wooden horse; a keyless cupboard, containing the various
implements of learning, a dirty duster, a pewter plate with
cretaceous deposits, a slop-basin and a ragged sponge;—and then,
unless he had included the cobwebs of the ceiling, (not usually
reckoned up in the furniture of a room,) no other movables
remained. One conspicuous fixture, however, there was, a gigantic
Dutch stove. This lumbering parallelogram, faggot-fed from the
corridor behind, projected several feet into the room, and shone
bright in the glaze of earthenware emblazonments. Around it we
would sometimes congregate in the intervals of class: in winter to
toast our hands and hind quarters, as we pressed against the heated
tiles, with more or less vigour according to the fervency of the
central fire; and in summer either to tell stories, or to con over the
pictorial History of the Bible, which adorned its frontispiece and
sides. We cannot say that every square exactly squared with even
our schoolboy notions of propriety in its mode of teaching religious
subjects; there was a Dutch quaintness in the illustrations, which
would sometimes force a smile from its simplicity, at others shock,
from its apparent want of decorum and reverence. Preeminent of
course among the gems from Genesis, Adam and Eve, safe in
innocency and "naked truth," here walked unscathed amidst a
menagerie of wild beasts—there, dressed in the costume of their fall,
they quitted Eden, and left it in possession of tigers, bears, and
crocodiles. Hard by on a smaller tile, that brawny "knave of clubs,"
Cain, battered down his brother at the altar; then followed a long
picture-gallery of the acts of the patriarchs, and another equally long
of the acts of the apostles. But, queer as many of these
misconceptions might seem, they were nothing to the strange
attempts made at dramatising the parables of the New Testament—
e. g. a stout man, staggering under the weight of an enormous
beam which grows out of one eye, employs his fingers, assisted by
the other, to pick out a black speck from the cornea of his neighbour.
Here, an unclean spirit, as black as any sweep, issues from the
mouth of his victim, with wings and a tail! Here again, the good
Samaritan, turbaned like a Turk, is bent over the waylaid traveller,
and pours wine and oil into his wounds from the mouths of two
Florence flasks; there, the grain of mustard-seed, become a tree,
sheltering already a large aviary in its boughs; the woman, dancing
a hornpipe with the Dutch broom, has swept her house, and lo! the
piece of silver that was lost in her hand; a servant, who is digging a
hole in order to hide his lord's talent under a tree, is overlooked by a
magpie and two crows, who are attentive witnesses of the deposit:—
and many others too numerous to mention. So much for the empty
school-room, but what's a hive without bees, or a school-room
without boys? The reader who has peeped into it untenanted, shall
now, if he pleases, be introduced, dum fervet opus full and alive.
Should he not be able to trace out very clearly the system at work,
he will at least be no worse off than the bee-fancier, who hears
indeed the buzzing, and sees a flux and reflux current of his winged
confectioners entering in and passing out, but cannot investigate the
detail of their labours any farther. In the Yverdun, as in the
hymenopterus apiary, we swarmed, we buzzed, dispersed,
reassembled at the sound of the bell, flocked in and flocked out, all
the day long; exhibited much restlessness and activity, evincing that
something was going on, but what, it would have been hard to
determine. Here the comparison must drop. Bees buzz to some
purpose; they know what they are about; they help one another;
they work orderly and to one end,—
FOOTNOTES:
[14] Cicero, De Fin., ii. 1.
THE CROWNING OF THE COLUMN,
AND CRUSHING OF THE PEDESTAL.
It was said in the debate on the Navigation Laws, in the best speech
made on the Liberal side, by one of the ablest of the Liberal party,
that the repeal of the Navigation Laws was the crowning of the
column of free trade. There is no doubt it was so; but it was
something more. It was not only the carrying out of a principle, but
the overthrow of a system; it was not merely the crowning of the
column, but the crushing of the pedestal.
And what was the system which was thus completely overthrown,
for the time at least, by this great triumph of Liberal doctrines? It
was the system under which England had become free, and great,
and powerful; under which, in her alone of all modern states, liberty
had been found to coexist with law, and progress with order; under
which wealth had increased without producing divisions, and power
grown up without inducing corruption; the system which had
withstood the shocks of two centuries, and created an empire
unsurpassed since the beginning of the world in extent and
magnificence. It was a system which had been followed out with
persevering energy by the greatest men, and the most commanding
intellects, which modern Europe had ever produced; which was
begun by the republican patriotism of Cromwell, and consummated
by the conservative wisdom of Pitt; which had been embraced alike
by Somers and Bolingbroke, by Walpole and Chatham, by Fox and
Castlereagh; which, during two centuries, had produced an
unbroken growth of national strength, a ceaseless extension of
national power, and at length reared up a dominion which embraced
the earth in its grasp, and exceeded anything ever achieved by the
legions of Cæsar, or the phalanx of Alexander. No vicissitudes of
time, no shock of adverse fortune, had been able permanently to
arrest its progress. It had risen superior alike to the ambition of
Louis XIV. and the genius of Napoleon; the rude severance of the
North American colonies had thrown only a passing shade over its
fortunes; the power of Hindostan had been subdued by its force, the
sceptre of the ocean won by its prowess. It had planted its colonies
in every quarter of the globe, and at once peopled with its
descendants a new hemisphere, and, for the first time since the
creation, rolled back to the old the tide of civilisation. Perish when it
may, the old English system has achieved mighty things; it has
indelibly affixed its impress on the tablets of history. The children of
its creation, the Anglo-Saxon race, will fill alike the solitudes of the
Far West, and the isles of the East; they will be found equally on the
shores of the Missouri, and on the savannahs of Australia; and the
period can already be anticipated, even by the least imaginative,
when their descendants will people half the globe.
It was not only the column of free trade which has been crowned in
this memorable year. Another column, more firm in its structure,
more lasting in its duration, more conspicuous amidst the wonders
of creation, has, in the same season, been crowned by British hands.
While the sacrilegious efforts of those whom it had sheltered were
tearing down the temple of protection in the West, the last stone
was put to the august structure which it had reared in the East. The
victory of Goojerat on the Indus was contemporary with the repeal
of the Navigation Laws on the Thames. The completion of the
conquest of India occurred exactly at the moment when the system
which had created that empire was repudiated. Protection placed the
sceptre of India in our hands, when free trade was surrendering the
trident of the ocean in the heart of our power. With truth did Lord
Gough say, in his noble proclamation to the army of the Punjaub, on
the termination of hostilities, that "what Alexander had attempted
they had done." Supported by the energy of England, guided by the
principles of protection, restrained by the dictates of justice, backed
by the navy which the Navigation Laws had created, the British arms
had achieved the most wonderful triumph recorded in the annals of
mankind. They had subjugated a hundred and forty millions of men
in the Continent of Hindostan, at the distance of ten thousand miles
from the parent state; they had made themselves felt alike, and at
the same moment, at Nankin, the ancient capital of the Celestial
Empire, and at Cabool, the cradle of Mahommedan power.
Conquering all who resisted, blessing all who submitted, securing
the allegiance of the subjects by the justice and experienced
advantages of their government, they had realised the boasted
maxim of Roman administration—
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