Unit-1 BE pdf
Unit-1 BE pdf
UNIT-1
FROM ATOMS TO ORGANISMS
The cell: The basic unit of life
• Cells are the fundamental structural and functional unit of all living beings.
• Every cell has its own life.
• Old and week cells in the body continually die and are replaced by new cells.
Cell theory
Cell theory is a scientific theory which describes the properties of cells. Cell theory was
eventually formulated in 1838. This is usually credited to Schleiden and Theodor Schwann.
The three tenets to the cell theory are as described below:
Cell types
Prokaryotic cell
Eukaryotic cell
Capsule: The capsule is found in some bacterial cells, this additional outer covering protects
the cell when it is engulfed by phagocytes and by viruses, assists in retaining moisture, and
helps the cell adhere to surfaces and nutrients.
Cell wall: Cell wall is the outermost layer of most cells that protects the bacterial cell and
gives it shape.. Bacterial cell walls are made of peptidoglycan which is made from
polysaccharide chains cross-linked by unusual peptides containing D-amino acids. The cell
wall of bacteria is also distinct from that of Archaea, which do not contain peptidoglycan.
The cell wall is essential to the survival of many bacteria. The antibiotic penicillin is able to
kill bacteria by preventing the cross-linking of peptidoglycan and this causes the cell wall to
weaken and lyse. Lysozyme enzyme can also damage bacterial cell walls.
Cell membrane: Cell membrane surrounds the cell's cytoplasm and regulates the flow of
substances in and out of the cell.
Cytoplasm: The cytoplasm of a cell is a fluid in nature that fills the cell and is composed
mainly of 80% water that also contains enzymes, salts, cell organelles, and various organic
molecules.
Ribosomes: Ribosomes are the organelles of the cell responsible for protein synthesis.
Nucleiod Region: The nucleoid region is possessed by a prokaryotic bacterial cell. It is the
area of the cytoplasm that contains the bacterial DNA molecule.
Plasmids: A plasmid is a DNA molecule (mostly in bacteria) that is separate from. They are
otherwise denoted as extra piece of chromosomal DNA. They are double-stranded and
circular. Plasmids are responsible for 1. Virulence factor 2. Antibiotic resistance 3.
Conjugation: The process of transferring genetic material from one bacterial cell to another
bacterial cell.
Pili: Pili are hair-like structures on the surface of the cell that help attach to other bacterial
cells. Shorter pili called fimbriae help bacteria attach to various surfaces. A pilus is typically
6 to 7 nm in diameter.
Flagella: It is a long, whip-like protrusion found in most prokaryotes that aids in cellular
locomotion. Besides its main function of locomotion it also often functions as a sensory
organelle, being sensitive to chemicals and temperatures outside the cell. The bacterial
flagellum is made up of the protein flagellin.
Cell Wall: It is the extracellular structure surrounding plasma membrane. The cell wall is
composed of cellulose, hemicellulose and pectin. An important function of the cell wall is
that it controls turgidity.
Cell membrane: The cell membrane is semipermeable, allowing selective substances to pass
into the cell and blocking others.
Chloroplasts: The plastids are chloroplasts, which contain chlorophyll and the biochemical
systems for light harvesting and photosynthesis.
Mitochondria: These are spherical to rod-shaped organelles with a double membrane. The
inner membrane is infolded many times, forming a series of projections (called cristae). The
mitochondrion converts the energy stored in glucose into ATP (adenosine triphosphate) for
the cell.
Nucleus: They are spherical body containing many organelles, including the nucleolus. The
nucleus controls many of the functions of the cell (by controlling protein synthesis) and
contains DNA (in chromosomes). The nucleus is surrounded by the nuclear membrane and
possesses the nucleolus which is an organelle within the nucleus - it is where ribosomal RNA
is produced.
Endoplasmic reticulum: Endoplasmic reticulum is the sites of protein maturation and they
can be divided into the following types:
Lysosome: Lysosomes are cellular organelles that contain the hydrolase enzymes which
breaks down waste materials and cellular debris. They can be described as the stomach of the
cell.
Peroxisome: Peroxisomes are organelles that contain oxidative enzymes. Peroxisomes
function to rid the body of toxic substances like hydrogen peroxide, or other metabolites.
They are a major site of oxygen utilization.
Centrosome: They are small body located near the nucleus. The centrosomes are the
destination where microtubules are made. During mitosis, the centrosome divides and the two
parts move to opposite sides of the dividing cell.
Vacuoles: They act as storage areas.
Lipids
Lipids are insoluble organic compounds that consist of fat and oil. The roles of lipids in cells
are energy storage, major component of cell membrane and cell signaling.
Simple Lipids or Homolipids: Simple lipids are esters of fatty acid linked with various
alcohols.
Nucleic acids
Nucleic acids are biopolymers, or large biomolecules, essential for all known forms of life.
Nucleic acids, which include DNA (deoxyribonucleic acid) and RNA (ribonucleic acid), are
made from monomers known as nucleotides. Each nucleotide has three components: a 5-
carbon sugar, a phosphate group, and a nitrogenous base.
DNA RNA
Nitrogenous bases Adenine (A), Guanine (G), Cytosine Adenine (A), Guanine (G), Cytosine (C)
(C)
Uracil (U)
Thymine (T)
Proteins
While nucleic acids carry the genetic information of the cell, the primary responsibility of
proteins is to execute the tasks directed by that information. Each cell contains different
proteins. They perform a wide variety of functions. Proteins direct virtually all activities of
the cell. Greek word proteios means ―of the first rank‖.
Functions of proteins: Serve as structural components of cells and tissues, transport and
storage of small molecules, transmitting information between cells, providing a defense
against infection. They act as enzymes. Catalyze nearly all the chemical reactions in
biological systems.
1 NEUTRAL: Amino acids with 1 amino group and 1 Glycine (Gly), Alanine (Ala), Valine (Val),
carboxyl group
Leucine (Leu), Isoleucine (Ile)
5 ALCOHOLIC: Amino acids having –OH group Serine (Ser), Threonine (Thr), Tyrosine (Tyr)
7 HETEROCYCLIC: Amino acids having nitrogen in ring Histidine (His), Proline (Pro)
structure
Essential amino acids: Histidine (His), Leucine (Leu), Isoleucine (Ile), Lysine (Lys),
Methionine (Met), Phenylalanine(Phe), Threonine (Thr), Tryptophan (Try), Valine (Val).
Non- essential amino acids: Glycine (Gly), Alanine (Ala), Aspartic acid (Asp), Asparagine
(Asn), Glutamic acid (Glu), Glutamine (Gln), Cysteine (Cys), Proline (Pro), Serine (Ser),
Tyrosine (Tyr).
Functions of proteins
Antibodies: Specialized proteins involved in defending the body from antigens.
Contractile Proteins: Responsible for movement. Involved in muscle contraction.
Eg: Haemoglobin
Cell metabolism
Metabolism: The sum of all chemical reactions that occur in the body. It requires the use of
enzymes. General Classification of chemical reactions
Anabolic - Those that create larger molecules
Catabolic - Those that breakdown larger molecules into smaller molecules
Cell metabolism / cell respiration produces Carbon dioxide, Water, ATP and Heat
Two types of reactions:
• Anaerobic reactions (without O2) – produce little ATP
• Aerobic reactions (requires O2) – produce most ATP
Glycolysis
Glycolysis is the central pathway for the glucose catabolism in which glucose (6-carbon
compound) is converted into pyruvate (3-carbon compound) through a sequence of 10 steps.
Glycolysis takes place in both aerobic and anaerobic organisms and is the first step towards
the metabolism of glucose. It occurs in cytosol. It yields two ATP molecules per glucose
molecule.
Electron-Transport Chain
It is the final step in energy generation – most energy released in this step. Electrons of
NADH and FADH2 move through the chain, moving to lower energy level. It occurs in the
inner membrane of the mitochondria. Specialized molecules accept and donate e- as they
move down chain. It creates an electrochemical gradient. As e- move down chain, H+ move
across the membrane, altering the concentration of H+ on either side = gradient. Gradient
used to generate ATP.
Energy released by movement of electrons through transport system is stored temporarily in
H+ gradient. NADH produces a maximum of 2.5 ATP. FADH2 produces a maximum of 1.5
ATP. 1 ATP formed per 3H+ shuttled through ATP synthase. The end of cellular respiration –
It yields 30-32 ATP molecules.
1. Prophase-I: It occupies the longest duration in Meiosis-I.It is divided into five sub-
stages or sub-phases.
Leptotene: This phase starts immediately after interphase. The size of cell and nucleus
increases. The chromosomes appear long, uncoiled thread-like in structure bearing many
bead-like structures called chromomeres. The nuclear membrane and nucleolus remain as it
is.
Zygotene: Homologous chromosomes come closer and start to pair up along their length.
The pairing of homologous chromosomes is called Synapsis and the paired homologous
chromosomes are referred as bivalents.
Pachytene: The chromosome becomes shorter and thicker. Each chromosome of the
bivalents splits longitudinally to form two chromatids such that bivalents is composed of four
strands and is known as a tetrad. The process of crossing over starts (crossing over; a small
fragment of chromosome exchange between two non-sister chromatids)
Diplotene: In this stage crossing over takes place. Exchange between homologous
chromosomes (two non-sister chromatids) occurs. Then they begin to separate.
Diakinesis: By the end of diakinesis the nuclear membrane and nucleolus get completely
disappeared and the chromosomes are free in the cytoplasm. Spindle fibres begin to form
2. Metaphase-I: The spindle fibres organized between two poles and get attached to the
centromere of chromosomes. Chromosome moves to equator. The bivalent
chromosomes are arranged in the equatorial plate in such a way that 2 metaphasic
plates are formed.
3. Anaphase-I: Spindle fibres contracts and pulls the whole chromosomes to the polar
region. The separated chromosome is known as dyads. Now the separated
chromosome moves toward opposite poles.
4. Telophase-I: Two groups of chromosome formed at each pole and organized into
nuclei. The nuclear membrane and nucleolus reappears. The chromosomes get
uncoiled into chromatin thread. The spindle fibres disappear totally.
Interphase II: The two cells or nuclei thus formed pass through a short stage called
interphase-II. Sometimes, interphase-II is absent. It is the resting phase between meiosis-I
and meiosis-II. It is either very short or may be absent. No DNA synthesis occurs.
Meiosis-II
Meiosis-II is exactly similar to mitosis, so it is also known as meiotic mitosis. In this division,
two haploid chromosomes split longitudinally and distributed equally to form 4 haploid cells.
It completes in 4 stages
1. Prophase-II: The dyads chromosome becomes thicker and shorter. Nuclear membrane and
nucleolus disappear. Spindle fibre starts to form
2. Metaphase-II: The dyads chromosomes come to equatorial plane. Spindle fibres organize
between poles and attaches to centromere of chromosome.
Cell differentiation
Cell differentiation may simply be described as the process through which a young and
immature cell evolves in to a specialized cell, reaching its mature form and function. For
such unicellular organisms like bacteria, various life functions occur within a single cell. That
is, such processes as the transport of molecules, metabolism and reproduction all take place
within a single cell given that they are single celled. However, multicellular
organisms require different types of cells for these processes to be possible. Multicellular
organisms begin as undifferentiated masses of cells. Variation in DNA expression and gene
activity determine the differentiation of cells and ultimately their specialization.
All cells contain the same DNA so cells initially have the potential to become any type of
cell. Cell Differentiation is irreversible. All cells in multicellular organism have the same
number of chromosomes and DNA. It influenced by the cell’s environment. Chemical signals
may be released by one cell to influence the development and activity of another cell.
Here, different types of cells play a specific function given that they have varied structures.
For instance, the nerve cells play a crucial role in the transmission of signals to different parts
of the body, blood cells play an important role carrying oxygen to different parts of the body.
The differences in structure and functions between the cells mean that they are specialized
cells. To be able to perform different functions, cells have to become specialized. This
becomes possible through the process referred to as cell specialization.
Specialized cells
Nerve Cells communicate information either by using electric signals (within a cell) or
chemical signals (between cells).
Muscle cells contain protein filaments that slide past one another, producing a contraction
that changes both the length and the shape of the cell
Blood cells are the most common type of blood cell and the vertebrate organism's principal
means of delivering oxygen to the body tissues
Xylem is the long trachea elements that transport water in a plant.
Phloem is part of a plant that carries food down the stem, and carries sugar, and protein to all
parts of the plant that need them
Stem Cells: They are otherwise called as blank cells. Unspecialized but can give rise to
specialized cells. When a stem cell divides, each new cell has the potential either to remain a
stem cell or become another type of cell with a more specialized function