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Class 9 Experiment- 1

The document outlines a procedure for preparing a stained temporary mount of onion peel to study its cells, detailing the materials required and the steps involved. Observations include the shape, arrangement, and presence of cell structures such as the nucleus and cell wall. The results indicate that the onion peel cells are rectangular, compactly arranged, and form the epidermis of the leaf.

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9 views3 pages

Class 9 Experiment- 1

The document outlines a procedure for preparing a stained temporary mount of onion peel to study its cells, detailing the materials required and the steps involved. Observations include the shape, arrangement, and presence of cell structures such as the nucleus and cell wall. The results indicate that the onion peel cells are rectangular, compactly arranged, and form the epidermis of the leaf.

Uploaded by

chadhah611
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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Onion Cell

AIM:
To prepare a stained, temporary mount of onion peel and to study its cells.

MATERIALS REQUIRED:
An onion bulb, slides, cover slips, two watch glasses, needle, brush, forceps, razor blade,
compound microscope, blotting paper, methylene blue (or safranin) solution, glycerine, and
water.

THEORY:
All living organisms are composed of cells. New cells arise by the division of pre-existing cells.
Cell is the structural and functional unit of life. In plants, cells have an outermost rigid cell wall
beneath which is a cell membrane. The cell membrane encloses cytoplasm, cell organelles,
and a nucleus.

PROCEDURE:
1. Take one fleshy scale leaf of an onion. Break it into two and using a forceps pull out a thin
membranous peel adhering to the inner surface of the leaf. This is the epidermal peel.
2. Place the peel in a watch glass containing water and cut it into small rectangular pieces.
3. Mix 1 or 2 drops of safranin in a small quantity of water taken in another watch glass and
transfer the peels into it. Leave the peels for about 3 minutes. Dip the peel in water to remove
excess stain.
4. Take a clean slide with a drop of glycerine in the middle and using a brush transfer the
washed and stained peel onto it.
5. Place a cover slip over it by slowly lowering it with a needle. Avoid the entry of air bubbles.
6. Remove excess glycerine from the edges of the cover slip with the help of a piece of blotting
paper.
7. Observe the slide under the microscope, first in low power and then in high power.
8. Draw a labeled diagram of the cells as seen under a microscope.
9. Note the features listed in the observation table.
OBSERVATIONS
OBSERVATION TABLE

Sl.No. Feature Observation

1. Shape of cells spherical/oval/rectangular/square

2. Arrangement of cells compact/loose

3. Intercellular spaces present/absent

4. Nucleus present/absent

5. Cell wall present/absent

6. Stained portions of cell cell wall/cytoplasm/nucleus

7. Unstained portions of cell cell wall/cytoplasm/vacuole

RESULT:
1. The cells that form the peel are rectangular in shape, compactly arranged and without any
intercellular spaces.
2. Each cell has a distinct cell wall, a prominent nucleus and a vacuole.
3. The cells form the outer layer of the leaf known as epidermis.

PRECAUTIONS:
1. Staining of the peel must be appropriate. Excess stain can be removed by rinsing the
peel with water taken in a watch glass.
2. Use a brush to transfer the peel onto the slide.
3. While placing the cover slip care should be taken to avoid air bubbles.

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