FORMULATION AND EVALUATION OF

HERBAL HAIR OIL

A Project Report Submitted

in Partial Fulfilment of the Requirements
for the Degree of

BACHELOR OF PHARMACY
By

YASH CHAUHAN
R. No. 211650300063

Under the Supervision of

DR. AMIT SEMWAL

Prof.

College of Pharmacy
Shivalik Campus, Dehradun

to the

Veer Madho Singh Bhandari

Uttarakhand Technical University, Dehradun
2024- 2025
 Certificate

This is to certify that YASH CHAUHAN student of B. Pharm 8th Semester at College of

Pharmacy, Shivalik Campus, Dehradun, has worked on the topic entitled “FORMULATION

AND EVALUTION OF HERBAL HAIR OIL ” under my supervision and guidance for the

partial fulfillment of requirement for the degree of Bachelor in Pharmacy (Session 2021-25).

Date:

Dr.Amit samwal
(Prof)
 Certificate

This is to certify that YASH CHAUHAN student of B. Pharm 8th Semester at College of

Pharmacy, Shivalik Campus, Dehradun, has worked on the topic entitled “FORMULATION

AND EVALUTION OF HERBAL HAIR OIL” for the partial fulfillment of requirement for the

degree of Bachelor in Pharmacy (Session 2021-25) of Veer Madho Singh Bhandari Uttarakhand

Technical University, Dehradun, Uttarakhand.

Date:

Prof. (Dr) Sayantan Mukhopadhyay

Principal
 Declaration by Student

I hereby declare that the project report on work entitled “FORMULATION AND EVALUTION

OF HERBAL HAIR OIL” done by me in fulfillment of the requirements for the award of degree

of Bachelor in Pharmacy is an authentic record of bonafide research work carried out by me under

the expert guidance as well as direct supervision of Dr. Amit Semwal (Prof.) I further declare

that the material obtained from other sources has been duly acknowledged in this project work

report.

Name: YASH CHAUHAN

University Roll No.: 211650300063
Batch: 2021-25
 Certificate by Examiner

This is to certify that the project work entitled “FORMULATION AND EVALUTION OF

HERBAL HAIR OIL” carried out by “YASH CHAUHAN” was examined by me.

Remarks (if any):

Examiner
Name & Designation with Signature
Acknowledgement

I would like to express my sincere gratitude and appreciation to all those who have contributed to
the successful completion of this project.

First and foremost, I would like to extend my heartfelt thanks to my project supervisor, Ms. ----
for their invaluable guidance, continuous support, and expertise throughout the duration of this
project. Their insightful feedback and constructive suggestions greatly enhanced the quality of my
work.

I would also like to thank the staff and faculty of the College of Pharmacy, Shivalik Campus for
providing the necessary resources and facilities that were crucial for the accomplishment of this
project. Their commitment to fostering a conducive learning environment has been instrumental
in my academic journey.

Furthermore, I am grateful to my family and friends for their unwavering encouragement, patience,
and understanding during this project. Their love, support, and belief in my abilities have been a
constant source of motivation and inspiration.

Lastly, I would like to acknowledge all the authors, researchers, and scholars whose work served
as a foundation and reference for this project. Their pioneering efforts and scholarly contributions
have shaped the field and provided invaluable knowledge.

In conclusion, I am deeply grateful to everyone who played a role, no matter how big or small, in
the successful completion of this project. Your support, guidance, and contributions have been
invaluable, and I am truly indebted to each and every one of you.

Thank you.
 Table of Contents

S. No. Chapter Page No.

1. Introduction 2-4

2. Literature Review 5-8

3. Aim and Objectives 9-10

4. Plan of Work 11-12

5. Materials and Methods 13-15

6. Evaluation 16-18

7. Result and discussion 19-21

8. Conclusion 22

9. References 23-25
Research publication, conference, patent
10.
certificates etc.

List of figures
S.NO Title Page No.
1. Methodology 15
2. Result 20
 List of tables

Title Page No.

S. No.
1. Ingredient 14
2. Evaluation 18
3. Result 20
 ABSTRACT

ABSTRACT
With rising awareness of the adverse effects of synthetic hair care products, there is increasing
interest in natural formulations. This study presents the formulation and evaluation of a herbal hair
oil using select ingredients known for their traditional and therapeutic benefits in hair and scalp
care. The formulation includes Cocos nucifera (Coconut oil) as the primary base for its nourishing
properties, complemented by Olea europaea (Olive oil) to support scalp health and enhance light
hair tones. Matricaria chamomilla (Chamomile) and Calendula officinalis (Calendula petals)
contribute natural yellow coloration and provide soothing, anti-inflammatory effects. Trigonella
foenum-graecum (Fenugreek) is included for root strengthening, and Tocopherol (Vitamin E oil)
acts as a natural preservative while promoting overall hair health.

The oil underwent physicochemical evaluations such as pH, viscosity, refractive index, acid value,
and saponification value. Stability testing was carried out under various environmental conditions
to estimate shelf life. Preliminary observations indicated favorable physical characteristics and
stability. The formulation showed promise in promoting hair health, with no signs of irritation in
skin safety assessments, making it a potentially safe and effective natural alternative for topical
hair care.

Keywords

Herbal hair oil, Eclipta alba, Emblica officinalis, hair growth, polyherbal formulation, natural
cosmetics, scalp health.

FORMULATION AND EVALUATION OF HERBAL HAIR OIL Page

1
 INTRODUCTION

CHAPTER 1
INTRODUCTION

FORMULATION AND EVALUATION OF HERBAL HAIR OIL Page

2
 INTRODUCTION

Introduction

Herbal hair oils have been used for centuries in traditional medicine systems such as Ayurveda,
Unani, and Siddha to promote hair growth, reduce hair fall, prevent dandruff, and maintain overall
scalp health. With increasing awareness of the adverse effects associated with synthetic hair care
products, there is growing consumer preference for herbal-based formulations due to their
perceived safety, efficacy, and minimal side effects (1)

Hair oil acts as a vehicle to deliver active constituents directly to the hair follicles and scalp. Oils
such as coconut, sesame, castor, and almond are commonly used as bases due to their emollient
properties, which help nourish and condition the hair shaft. The incorporation of medicinal plant
extracts enhances the therapeutic value of these oils(.2,3) Plants like Bhringraj (Eclipta alba), Amla
(Emblica officinalis), Brahmi (Bacopa monnieri), Neem (Azadirachta indica), and Hibiscus
(Hibiscus rosa-sinensis) have been traditionally acclaimed for their hair growth-promoting
properties (4,5).

The formulation of herbal hair oil involves selecting appropriate plant materials, extracting their
bioactive constituents, and combining them in an oil base under controlled conditions. Evaluation
parameters such as physicochemical properties (e.g., viscosity, pH, refractive index), stability
studies, and efficacy assessments (e.g., hair growth studies in animal models or user trials) are
essential to ensure product quality and performance (5,6).

Thus, the present study focuses on the formulation and systematic evaluation of a polyherbal hair
oil using scientifically validated herbs, aiming to offer a natural and effective alternative for hair
care.( 7)

Advantages of Herbal Hair Oil

1. Promotes Hair Growth

Herbal hair oils containing ingredients like Eclipta alba, Hibiscus rosa-sinensis, and Bacopa
monnieri stimulate hair follicle activity and prolong the anagen (growth) phase of the hair cycle.(8)

FORMULATION AND EVALUATION OF HERBAL HAIR OIL Page

3
 INTRODUCTION

2. Reduces Hair Fall and Strengthens Roots

Herbs such as Amla (Emblica officinalis) and Bhringraj are rich in antioxidants and vitamin C,
which help strengthen hair roots and reduce oxidative stress-related hair fall.(9)

3. Prevents Dandruff and Scalp Infections

Herbs like Neem (Azadirachta indica) and Tulsi (Ocimum sanctum) possess strong antimicrobial,
antifungal, and anti-inflammatory properties that help combat dandruff and maintain a healthy
scalp.

4. Improves Hair Texture and Shine

Coconut oil used as a base in herbal hair oils improves hair texture, reduces protein loss, and adds
natural shine by penetrating the hair shaft.

5. Free from Harmful Chemicals

Herbal hair oils are generally free from synthetic preservatives, parabens, sulfates, and artificial
fragrances, reducing the risk of allergic reactions and scalp irritation.(10,11)

6. Cost-effective and Eco-friendly

Herbal hair oils can often be produced from locally available plants, making them an affordable
and sustainable alternative to chemical-based commercial products.

7. Safe for Long-term Use

Herbal products generally have a low toxicity profile and are suitable for prolonged use without
causing adverse effects, especially when prepared using traditional methods.(12)

FORMULATION AND EVALUATION OF HERBAL HAIR OIL Page

4
 LITRATURE AND REVIEW

CHAPTER 2
LITERATURE REVIEW

FORMULATION AND EVALUATION OF HERBAL HAIR OIL Page

5
 LITRATURE AND REVIEW

Literature review

The use of herbal formulations for hair care has been deeply rooted in various traditional systems
of medicine. With the increasing demand for natural products and the growing concerns over
synthetic cosmetic ingredients, numerous studies have been conducted to explore the potential of
herbs in hair oil formulations. The following literature provides insights into the individual herbs
commonly used, their pharmacological activities, formulation techniques, and evaluation
parameters.

1.Traditional Herbs in Hair Oil Formulations

Numerous herbs are used for their beneficial effects on hair growth, dandruff control, and scalp
nourishment:

Eclipta alba (Bhringraj): Commonly known as "king of hair," Eclipta alba has shown significant
hair growth-promoting activity in several animal studies. Roy et al. (2008) reported that methanolic
extracts of Eclipta alba stimulated hair follicles in the anagen phase and increased hair length
significantly in albino rats.

Emblica officinalis (Amla): Rich in vitamin C and tannins, Amla strengthens hair follicles and
prevents premature graying. It is widely used in Ayurvedic hair care formulations. According to
Kapoor et al. (2011), Amla extracts have antioxidant and hair conditioning properties.

Hibiscus rosa-sinensis: Hibiscus flowers and leaves are known to promote hair growth and act as
natural conditioners. A study by Adhirajan et al. (2003) demonstrated that petroleum ether extracts
of Hibiscus rosa-sinensis significantly increased hair length and weight in test animals.

FORMULATION AND EVALUATION OF HERBAL HAIR OIL Page

6
 LITRATURE AND REVIEW

Azadirachta indica (Neem): Due to its antifungal, antibacterial, and anti-inflammatory properties,
Neem is effective in treating dandruff and scalp infections. Kaur et al. (2010) highlighted Neem
oil's potential in maintaining scalp hygiene and reducing microbial growth.

Cocos nucifera (Coconut oil): Used as a base oil, it penetrates the hair shaft and reduces protein
loss from hair. Rele and Mohile (2003) demonstrated that coconut oil was more effective than
mineral and sunflower oil in preventing hair damage.

2.Herbal Oil Formulation Studies

Several studies have been conducted on the formulation of polyherbal hair oils:

Rathod et al. (2010) formulated a polyherbal hair oil containing Bhringraj, Amla, Brahmi, and
Neem. The formulation was tested for various parameters including pH, viscosity, and hair growth
activity. The oil showed significant improvement in hair length and hair follicle number compared
to control.

Pakhale et al. (2012) developed an herbal hair oil using Hibiscus, Amla, Shikakai, and Reetha.
Their formulation was evaluated for hair softening, conditioning, and non-irritating properties, all
of which were found to be within acceptable ranges.

Prajapati et al. (2011) studied the effects of various herbal oils on hair growth in albino rats. They
found that herbal oils containing Eclipta alba and Cocos nucifera enhanced hair length and density
significantly when applied over a 21-day period.

3. Evaluation Parameters of Herbal Hair Oils

FORMULATION AND EVALUATION OF HERBAL HAIR OIL Page

7
 LITRATURE AND REVIEW

To assess the effectiveness and safety of herbal hair oils, a wide range of physicochemical and
biological parameters are evaluated:

Physicochemical Tests: These include pH, viscosity, density, refractive index, and acid value.
These parameters ensure the oil is stable, safe, and suitable for topical application (Kokate et al.,
2010).

Stability Studies: Herbal oils are subjected to accelerated storage conditions to evaluate stability
over time. This includes monitoring changes in color, odor, consistency, and phase separation
(Deshmukh et al., 2012).

Hair Growth Activity: Hair oil is tested on animal models (commonly albino rats or mice) to
evaluate hair length, hair follicle density, and hair growth phase. Histological studies are often
conducted to support these findings (Adhirajan et al., 2003).

Sensory Evaluation: User acceptability based on fragrance, texture, ease of application, and
rinsability is often assessed via volunteer studies.

4. Regulatory and Safety Considerations

Herbal products are often perceived as safe, but standardization and toxicity studies are crucial to
ensure quality. The absence of harmful preservatives, synthetic colors, and allergens must be
confirmed. WHO and AYUSH guidelines emphasize the need for validated protocols in herbal
drug formulations (WHO, 2007).

FORMULATION AND EVALUATION OF HERBAL HAIR OIL Page

8
 AIM AND OBJECTIVE

CHAPTER 3

AIM AND OBJECTIVE

FORMULATION AND EVALUATION OF HERBAL HAIR OIL Page

9
 AIM AND OBJECTIVE

AIM

To formulate and evaluate a safe and effective herbal hair oil using traditional medicinal plants for
promoting hair growth and improving scalp health.

OBJECTIVE

1. To identify and select medicinal plants traditionally used for promoting hair growth,
preventing hair fall, and improving scalp health.
2. To prepare extracts of selected herbs using suitable extraction techniques (e.g., maceration,
decoction).
3. To formulate a stable herbal hair oil using a suitable base oil (e.g., coconut, sesame, or
castor oil) incorporating the herbal extracts.
4. To evaluate the physicochemical parameters of the prepared herbal hair oil, including:
5. pH
6. Viscosity
7. Refractive index
8. Saponification value
9. Acid value
10. To assess the hair growth-promoting activity of the herbal hair oil through in vivo studies
using an animal model (e.g., albino rats), measuring parameters such as:
11. Hair length
12. Hair weight
13. Hair follicle density
14. To perform stability testing of the herbal hair oil under different storage conditions to
ensure its shelf-life and quality.
15. To conduct a preliminary safety and skin irritation test to evaluate the suitability of the
formulation for topical application.
16. To compare the effectiveness of the formulated herbal hair oil with marketed or standard
hair oil formulations.

FORMULATION AND EVALUATION OF HERBAL HAIR OIL Page

10
 RESULT AND DISCUSSION

CHAPTER 4
PLAN OF WORK

FORMULATION AND EVALUATION OF HERBAL HAIR OIL Page

11
 RESULT AND DISCUSSION

1. Plan of Work
a. Literature survey on herbs used in hair care. Selection of effective herbs for hair oil formulation

b. Collection and authentication of selected herbal materials. Ensuring botanical accuracy and
quality of ingredients

c. Extraction of active constituents from selected herbs. Obtaining concentrated extracts for
formulation

d. Formulation of polyherbal hair oil. Stable herbal oil with therapeutic properties

e. Evaluation of physicochemical properties. Establishing quality standards of the oil

f. In vivo hair growth activity studies. Assessment of the efficacy in promoting hair growth

g. Stability studies under different conditions. Shelf-life prediction and formulation consistency

h. Skin irritation/safety studies. Ensuring dermatological safety of the formulation

I. Comparison with marketed formulation. Determining relative effectiveness and user

satisfaction

j. Compilation and analysis of data Interpretation of results and conclusion

FORMULATION AND EVALUATION OF HERBAL HAIR OIL Page

12
 RESULT AND DISCUSSION

CHAPTER 5

Material and Methods

FORMULATION AND EVALUATION OF HERBAL HAIR OIL Page

13
 RESULT AND DISCUSSION

S.No. Ingredient Botanical name purpose Quantity(ml or

mg)
1. Coconut Oil Cocos nucifera 70ml |
Base oil, scalp
nourishment

2. Olive Oil(light) Olea Europaea Enhances light tone, 20ml

scalp health
3. Chamomile Matricaria Natural pale yellow 2g
chamomilla color, soothing
4. Calendula petals Calendula Yellow tint, anti- 2g
(dried) officinalis inflammatory
5. Fenugreek (light use) Trigonella 1g
Root strengthening
foenum-graecum
6. Vitamin E oi Tocopherol Natural 1ml
preservative, hair
health

Ingridient Table:1

Methodology
1. Combine Oils:

In a clean heat-safe bowl, mix coconut oil and olive oil.

2. Add Herbs:

Add dried chamomile, calendula petals, and fenugreek seeds to the oil blend.

Infuse (Double Boiler Method):

Place the bowl over a pot of simmering water.

FORMULATION AND EVALUATION OF HERBAL HAIR OIL Page

14
 RESULT AND DISCUSSION

Heat gently for 30–45 minutes, stirring occasionally.

Maintain low heat to preserve color and prevent burning of herbs.

3. Cool and Filter:

Let the oil cool to room temperature.

Strain through muslin cloth or a fine sieve to remove all herbal residue.

4. Add Final Ingredients: Stir in Vitamin E, and essential oil.

5. Store: Transfer the oil into dark amber bottles and store in a cool, dry place.

FORMULATION AND EVALUATION OF HERBAL HAIR OIL Page

15
 RESULT AND DISCUSSION

CHAPTER 6
EVULATION

1. Evaluation:-

FORMULATION AND EVALUATION OF HERBAL HAIR OIL Page

16
 RESULT AND DISCUSSION

1.1 Selection of Herbal Components

Herbs will be selected based on both traditional uses and scientific evidence for their benefits in
promoting hair growth, preventing hair fall and dandruff, nourishing the scalp, and exhibiting
antimicrobial effect.
Eclipta alba (Bhringraj), Hibiscus rosa sinensis, Trigonella foenum graecum (Fenugreek),
Azadirachta indica (Neem), Phyllanthus emblica (Amla), Bacopa monnieri (Brahmi), along with
carrier oils like Cocos nucifera (Coconut oil) and Sesamum indicum (Sesame oil).(14,15)
1.2 Preparation of Herbal Extracts
1.Drying & Pulverization: The selected plant materials will be thoroughly washed, dried under
shade, and then ground into fine powder.
2. Extraction Techniques:

2.1 Decoction: Herbal powders are boiled in water to extract active constituents.

2.2 Infusion or Maceration: Herbs are soaked in oils such as coconut or sesame for a set period.

2.3 Soxhlet Extraction: Employed for producing concentrated extracts using suitable solvents.

2.3.1Base Oil Selection: Oils such as coconut, sesame, and castor oil may be used either alone or
in combination.

2.3.2 Preparation Process (16,17)

2.3.3 Weigh the required amounts of herbal powders or extracts.

2.3.4 Heat the chosen base oil in a stainless steel container to around 70–80°C.

2.3.5 Gradually incorporate the herbal ingredients while maintaining gentle heat and stirring
continuously.

2.3.6Continue heating the mixture for approximately 3–4 hours or until desired color and aroma
develop, indicating sufficient extraction.

2.3.7Filter the oil through a muslin cloth to remove any residues. (18,19)

2.3.8 Allow to cool and store in airtight, amber colored containers to protect from light.

2.4 Table 2. ( Physicochemical Analysis)

FORMULATION AND EVALUATION OF HERBAL HAIR OIL Page

17
 RESULT AND DISCUSSION

Property Assessment Method

Color & Aroma Visual inspection and sensory analysis

Viscosity Measured using a Brookfield viscometer

Specific Gravity Determined using a pycnometer

Refractive Index Measured with a refractometer

pH (for emulsified oils) Tested using a digital pH meter

Acid Value Titration to evaluate rancidity

Saponification Value Indicates the amount of alkali needed to saponify the oil

Iodine Value Reflects unsaturation level of oil

Peroxide Value Shows extent of oxidation

FORMULATION AND EVALUATION OF HERBAL HAIR OIL Page

18
 RESULT AND DISCUSSION

CHAPTER 5
RESULT AND DISCUSSION

1. organoleptic evulation
s.no parameter observation
1. color natural /herbal hue

FORMULATION AND EVALUATION OF HERBAL HAIR OIL Page

19
 RESULT AND DISCUSSION

2. odor characteristic /herbal aroma

3. consistency smothness
organoleptic evulation table 3

2. physicochemical tests

s.no test result

FORMULATION AND EVALUATION OF HERBAL HAIR OIL Page

20
 RESULT AND DISCUSSION

1. ph 4.5- 6.5

2. viscosity 80-100 cp
3. spread ability determines ease of
application on scalp
physicochemical tests table 4

3. stability studies

s.no condition duration

1. room temperature 2-4 week
2. accelerated 2-3 week
3. light exposure sunlight
stability studies table 5

Discussion

The formulated herbal hair oil demonstrated excellent organoleptic and physicochemical
characteristics. The use of traditional herbs like The formulation of herbal hair oil using a blend
of Cocos nucifera (Coconut oil), Olea europaea (Olive oil), Matricaria chamomilla
(Chamomile), Calendula officinalis, Trigonella foenum-graecum (Fenugreek),
and Tocopherol (Vitamin E) aimed to provide a natural and effective approach to hair and scalp
care. Each ingredient was carefully selected based on its documented therapeutic properties, and
the formulation process preserved the integrity of bioactive compounds.

FORMULATION AND EVALUATION OF HERBAL HAIR OIL Page

21
 CONCLUSION

CONCLUSION

With increasing consumer inclination toward natural and safer alternatives, the formulation of
herbal hair oil using traditionally acclaimed medicinal plants offers a promising solution to
common hair and scalp issues. This study successfully developed a polyherbal hair oil
utilizing Cocos nucifera (Coconut oil) as the primary base, enriched with Olea europaea
(Olive oil), Matricaria chamomilla (Chamomile), Calendula officinalis, Trigonella foenum-
graecum (Fenugreek), and Tocopherol (Vitamin E). Each component was selected for its well-
documented therapeutic benefits, including nourishment, anti-inflammatory action, scalp
conditioning, and root strengthening.

Overall, the herbal hair oil demonstrated potential as a natural, effective, and stable
formulation for maintaining scalp health, supporting hair growth, and minimizing the adverse
effects associated with synthetic hair care products. Further clinical trials and consumer-based
efficacy studies are recommended to validate its long-term benefits and commercial
applicability.

FORMULATION AND EVALUATION OF HERBAL HAIR OIL Page

22
 PLAGRISM REPORT

REFERENCE

1. Bhatia, A., Mital, N., & Sekhon, B. S. (2014). Herbal hair cosmetics: Advancements and
recent findings. International Journal of Trichology, 6(1), 2–6.
https://doi.org/10.4103/0974-7753.138579

2. Gupta, M., Sasmal, D., & Mukherjee, A. (2011). Validation of traditional claims of some
Indian medicinal plants for hair care. International Journal of Pharmacognosy and
Phytochemical Research, 3(3), 50–53.

3. Kumar, N., Singh, A. K., & Srivastava, A. K. (2013). Herbal hair cosmetics: An overview.
Research Journal of Topical and Cosmetic Sciences, 4(1), 8–14
4. Rathod, V. S., Mehta, D. M., & Panchal, S. S. (2010). Formulation and evaluation of
polyherbal hair oil. International Journal of Pharmaceutical Sciences Review and Research,
5(2), 30–33.

5. Adhirajan, N., Ravi, K., & Shanmugasundaram, N. (2003). In vivo and in vitro evaluation
of hair growth potential of Hibiscus rosa-sinensis Linn. Journal of Ethnopharmacology,
88(2-3), 235–239.

6. Bhatia, A., Mital, N., & Sekhon, B. S. (2014). Herbal hair cosmetics: Advancements and
recent findings. International Journal of Trichology, 6(1), 2–6.

7. Kapoor, V. P., Singh, R. P., & Singh, A. (2011). Antioxidant potential of Emblica officinalis
fruit extracts. Pharmacognosy Journal, 3(21), 90–94.

FORMULATION AND EVALUATION OF HERBAL HAIR OIL Page

23
 PLAGRISM REPORT

8. Kaur, S., & Arora, S. (2010). Antimicrobial activity of some Indian medicinal plants.
Journal of Ethnopharmacology, 130(1), 142–145.

9. Kokate, C. K., Purohit, A. P., & Gokhale, S. B. (2010). Pharmacognosy, 45th ed., Nirali
Prakashan, Pune.

10. Prajapati, S. K., Tripathi, P., & Gupta, A. (2011). Hair growth activity of herbal oil in albino
rats. International Journal of Pharmaceutical Sciences and Research, 2(7), 1711–1714.

11. Rathod, V. S., Mehta, D. M., & Panchal, S. S. (2010). Formulation and evaluation of
polyherbal hair oil. International Journal of Pharmaceutical Sciences Review and Research,
5(2), 30–33.

12. Rele, A. S., & Mohile, R. B. (2003). Effect of mineral oil, sunflower oil, and coconut oil
on prevention of hair damage. Journal of Cosmetic Science, 54(2), 175–192.

13. Roy, R. K., Thakur, M., & Dixit, V. K. (2008). Hair growth promoting activity of Eclipta
alba in male albino rats. Archives of Dermatological Research, 300(7), 357–364.

14. World Health Organization (WHO). (2007). Guidelines for assessing quality of herbal
medicines with reference to contaminants and residues. WHO Press.

15. Reference: Adhirajan, N., Ravi, K., & Shanmugasundaram, N. (2003). In vivo and in vitro
evaluation of hair growth potential of Hibiscus rosa-sinensis Linn. Journal of
Ethnopharmacology, 88(2–3), 235–239.

FORMULATION AND EVALUATION OF HERBAL HAIR OIL Page

24
 PLAGRISM REPORT

16. Reference: Kapoor, V. P., et al. (2011). Antioxidant potential of Emblica officinalis fruit
extracts. Pharmacognosy Journal, 3(21), 90–94.

17. Reference: Kaur, S., & Arora, S. (2010). Antimicrobial activity of some Indian medicinal
plants. Journal of Ethnopharmacology, 130(1), 142–145.

18. Reference: Rele, A. S., & Mohile, R. B. (2003). Effect of mineral oil, sunflower oil, and
coconut oil on prevention of hair damage. Journal of Cosmetic Science, 54(2), 175–192.

19. Reference: Bhatia, A., Mital, N., & Sekhon, B. S. (2014). Herbal hair cosmetics:
Advancements and recent findings. International Journal of Trichology, 6(1), 2–6.

20. Reference: Kumar, N., Singh, A. K., & Srivastava, A. K. (2013). Herbal hair cosmetics: An
overview. Research Journal of Topical and Cosmetic Sciences, 4(1), 8–14.

21. Reference: World Health Organization (WHO). (2007). Guidelines for assessing quality of
herbal medicines with reference to contaminants and residues.

FORMULATION AND EVALUATION OF HERBAL HAIR OIL Page

25
 PLAGRISM REPORT

CHAPTER 8

PLAGRISM REPORT

FORMULATION AND EVALUATION OF HERBAL HAIR OIL Page

26
 PLAGRISM REPORT

FORMULATION AND EVALUATION OF HERBAL HAIR OIL Page

27