Dissolution System Drivers

Operation Manual
81-200-807 Rev. D

Hanson Research Corporation

9810 Variel Avenue  Chatsworth, CA 91311 USA
(800) 821-8165  (818) 882-7266  FAX (818) 882-9470
www.hansonresearch.com 2012 Hanson Research Corporation.

This product is covered under one or more of the following US Patents: 3,572,648 4,108,602 4,274,286 5,198,109 5,296,139 5,639,953 5,639,974
5,682,001 6,006,777 6,076,410 6,422,098 6,821,419 B2 Des.377,152 Des.424,458 & Other Patents Pending.
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Hanson Dissolution System Drivers Operation Manual 81-200-807-D

Table of Contents
Table of Contents ........................................................................................................... i
Revision History ........................................................................................................... iii
1 Safety Considerations ............................................................................................ 1
2 Introduction .............................................................................................................. 2
2.1 Overview ........................................................................................................................ 2
2.2 Configurations .............................................................................................................. 3
2.2.1 SR8-Plus/AutoPlus/Agilent Configuration ................................................................................ 3
2.2.2 AutoPlus/Agilent/Non-Hanson Bath Configuration.................................................................. 3
2.2.3 SR8-Plus/Agilent/Non-Hanson Sampling System Configuration .......................................... 4
2.2.4 Vision G2 AutoPlus/Vision G2 Elite 8/Agilent Configuration ................................................. 4
2.2.5 Vision G2 AutoPlus/Agilent/Non-Hanson Bath Configuration ............................................... 5
2.2.6 Vision G2 Elite 8/Agilent/Non-Hanson Sampling System Configuration ............................. 5

3 Specifications .......................................................................................................... 6
3.1 Software Requirements............................................................................................... 6
3.2 Hardware Requirements ............................................................................................. 6
3.3 Optional Accessories .................................................................................................. 7
4 Installation ................................................................................................................ 8
4.1 Before You Begin ......................................................................................................... 8
4.2 Hardware Installation ................................................................................................ 10
4.2.1 SR8-Plus/AutoPlus/Agilent Configuration .............................................................................. 10
4.2.2 AutoPlus/Agilent/Non-Hanson Bath Configuration................................................................ 12
4.2.3 SR8-Plus/Agilent/Non-AutoPlus Sampling System Configuration ...................................... 15
4.2.4 Vision G2 AutoPlus/Vision G2 Elite 8/ Agilent Configuration .............................................. 16
4.2.5 Vision G2 AutoPlus/Agilent/Non-Hanson Bath Configuration ............................................. 18
4.2.6 Vision Elite 8/Agilent/Non-AutoPlus Sampling System Configuration................................21
4.3 Software Installation .................................................................................................. 22
4.3.1 Uninstalling Previous Installations of the Hanson Dissolution System Drivers.................22
4.3.2 Installing the Hanson Dissolution Drivers ............................................................................... 25

5 Operation and Programming ............................................................................... 30

5.1 Dissolution Driver ...................................................................................................... 30
5.1.1 Test Station Tab ......................................................................................................................... 32
5.1.2 Reference Tab ............................................................................................................................ 33
5.1.3 Setup Tab ....................................................................................................................................34
5.1.4 About Tab ....................................................................................................................................35
5.2 AutoPlus Driver .......................................................................................................... 36
Creating a ChemStation Method ........................................................................................ 40
5.2.1 Creating an AutoPlus Protocol (Optional) .............................................................................. 40

Table of Contents i
Hanson Dissolution System Drivers Operation Manual 81-200-807-D

5.2.2 Creating a ChemStation Dissolution Method ......................................................................... 40

5.2.3 Defining the Measurement Cycle ............................................................................................ 48
5.2.4 Defining the Control (Optional) ................................................................................................ 49
5.2.5 Setting Up the Sampling System ............................................................................................. 50

6 Running a Test and Reviewing Data .................................................................. 52

6.1 Zero the Cells (if done previously, go to Section 6.2) .......................................... 52
6.2 Spectrophotometer Reference (Multibath Dissolution Testing Only) ............... 54
6.3 Load or Run Standards ............................................................................................. 55
7 Troubleshooting .................................................................................................... 58
8 Maintenance and Calibration............................................................................... 60
9 Moving and Storage .............................................................................................. 61
10 Service Parts ....................................................................................................... 62
11 Sales and Support .............................................................................................. 63
12 General Warranty ............................................................................................... 64
Appendix A Dissolution Run Parameters ................................................................ 65
PreRun Seq. .......................................................................................................................... 65
Dissolution Run .................................................................................................................... 68
PostRun Seq. ........................................................................................................................ 70

Table of Contents ii
Hanson Dissolution System Drivers Operation Manual 81-200-807-D

Revision History
REVISION DATE DESCRIPTION

Operation Manual
A 28AUG2006  Initial Release of new operation manual.
B 15FEB2007  Updated Revision History.
C 10AUG2010  Updated to reflect Vision Tester interface.
 Updated to reflect Vision Enabled AutoPlus interface.
 Updated to reflect Chemstation 32 requirements.
 Miscellaneous contextual adjustments.
D 01JUL2012  Updated to reflect Vision G2 Tester Interface
 Updated to reflect Vision G2 AutoPlus Autosampler interface
 Updated to reflect Windows 7 compatibility and requirements

Dissolution Tester Driver

3.12 28AUG2006  New driver installation menu.
 Validated with ChemStation v10.01.
 Improved timing of speed and temperature log feature.
 Disabled stopping of paddles at start of test for sequential
autosamplers.

3.13 21DEC2006  Addressed installation program not installing file xdtpatch.mcx

in target computer.

3.14 04JAN2007  Resolved ChemStation not reading the RPM from driver
correctly when Regional Settings in Windows switched the use
of the comma and the period.

3.15 15FEB2007  Modifed driver to take over control of E-Probes when using a
peristaltic pump instead of AutoPlus.
4.00 28JUN2010  Revised driver to work with Vision Elite 8 Dissolution Tester.

5.00 01JUL2012  Revised for Windows 7 compatibility

 Removed Vision G1 tester compatibility
 Added Vision G2 tester interface
 Updated SR8 Plus interface

AutoPlus Driver
3.12 28AUG2006  New driver installation menu.
 Improved Zero Cells functionality for Multibath Mode.
 Repaired GPF error when more than 21 protocols are used.
 Added feature to document AutoPlus test parameters at start
of test with ability to print automatically or manually.

3.13 21DEC2006  Addressed installation program not installing file apdriver.exe

in target computer.

Revision History iii

Hanson Dissolution System Drivers Operation Manual 81-200-807-D

3.14 04JAN2007  Resolved AutoPlus parameters printing at every interval time

by printing only at the start of the test.

3.15 15FEB2007  No modification. Version number changed to match SR8-Plus

Driver.
4.00 28JUN2010  Revised driver to work with Vision Enabled AutoPlus
Autosampler.
 Fixed an issue where the last blank would be applied properly
at every other sampling time.

5.00 01JUL2012  Revised for Windows 7 compatibility

 Removed Vision Enabled AutoPlus compatibility
 Added Vision G2 AutoPlus interface
 Updated legacy AutoPlus interface

Revision History iv
Hanson Dissolution System Drivers Operation Manual 81-200-807-D

1 Safety Considerations
No safety considerations are applicable to the Hanson Dissolution System Drivers.

Refer to the appropriate operation manuals or other reference materials for the Safety
Considerations for each of the individual instruments operating in the system.

Section 1 Safety Considerations –1–

Hanson Dissolution System Drivers Operation Manual 81-200-807-D

2 Introduction
2.1 Overview
Hanson Dissolution System Drivers were designed by Hanson Research in conjunction with
Agilent Technologies specifically to provide a communication tool between the Agilent
ChemStation™ program and select Hanson Research instruments. The drivers provide the
mechanism by which the ChemStation can control and retrieve status information from
Hanson dissolution instruments such as the SR8-Plus™ and AutoPlus™, and the Vision G2
Elite™ Dissolution Test Station and Vision G2 AutoPlus™ automated samplers. This manual
refers to the SR8-Plus™ and AutoPlus™ as the Legacy instruments and to the Vision G2
Elite™ Dissolution Test Station and Vision G2 AutoPlus™ as the Vision instruments.

The driver connects as a DDE1 client to the Agilent ChemStation, establishing a

communication channel through which both the ChemStation program and the Hanson
drivers can exchange instructions and information. Instructions from the ChemStation are
interpreted and implemented by the Hanson drivers through either RS232 via the serial
communication port (for Legacy) or TCPI/P network port (for Vision) of the host workstation
PC.

The Hanson drivers act as a communication tool between the Agilent ChemStation and the
Hanson Research instruments. Because the Hanson drivers do not alter the functionality of
the ChemStation program, this operation manual does not address the ChemStation program
itself, but rather the operation of the Hanson drivers in the ChemStation environment. For
information on the ChemStation program, refer to the materials provided with the
ChemStation software or contact your Agilent representative.

The Hanson Dissolution System Drivers version 5.00 (p/n 81-200-026) are supplied by
Hanson Research via CD ROM. These have been validated by Hanson Research with
ChemStation Version B.04.02. Hanson Research has not validated version 5.00 drivers with
ChemStation Versions lower than B.04.02.

1
DDE stands for Dynamic Data Exchange. DDE is the way that applications running within the Microsoft® Windows® environment
communicate with each other.

Section 2 Introduction –2–

Hanson Dissolution System Drivers Operation Manual 81-200-807-D

2.2 Configurations
The Hanson/Agilent System can be configured in various ways depending on the equipment
that will be used on the system. In each case the individual instruments should be installed
and connected prior to installing the Hanson Dissolution System Drivers.

2.2.1 SR8-Plus/AutoPlus/Agilent Configuration

This configuration includes one, two, or three SR8-Plus Dissolution Test Stations connected
to the AutoPlus and the Agilent 8453 Spectrophotometer. This configuration will use both the
tester and sampler drivers. See Figure 2-1 for an example setup of this configuration.

Figure 2-1 SR8-Plus/AutoPlus/Agilent Configuration

2.2.2 AutoPlus/Agilent/Non-Hanson Bath Configuration

This configuration includes one, two, or three Non-Hanson Dissolution Test Stations
connected to the AutoPlus and the Agilent 8453 Spectrophotometer. This configuration will
use only the sampler driver. Control of the baths will require a driver from the bath vendor or
other source, if available. See Figure 2-2 for an example setup of this configuration.

Figure 2-2 AutoPlus/Agilent/Non-Hanson Bath Configuration

Section 2 Introduction –3–

Hanson Dissolution System Drivers Operation Manual 81-200-807-D

2.2.3 SR8-Plus/Agilent/Non-Hanson Sampling System Configuration

This configuration includes one, two, three, or four SR8-Plus Dissolution Test Stations
connected to an Agilent 8453 Spectrophotometer with a non-AutoPlus sampling system such
as the Agilent peristaltic pump/valve system. This configuration will use only the tester driver.
Control of the sampling system will require a driver from the vendor, if available, for the
sampling system or, in the case of the Agilent peri-pump/valve system, the control is
incorporated into the Agilent ChemStation software. See Figure 2-3 for an example setup of
this configuration.

Figure 2-3: SR8-Plus/Agilent/Non-AutoPlus Sampling System Configuration

Other accessories and options may be included in a particular system that will change the
actual layout of the systems shown.

2.2.4 Vision G2 AutoPlus/Vision G2 Elite 8/Agilent Configuration

This configuration includes one, two, or three Vision G2 Elite 8 Dissolution Test Stations
connected to the Vision G2 AutoPlus and the Agilent 8453 Spectrophotometer. This
configuration will use both the tester and sampler drivers. See Figure 2-4 for an example
setup of this configuration.

Figure 2-4: Vision G2 AutoPlus/Vision G2 Elite 8 Tester/Agilent Configuration

Section 2 Introduction –4–

Hanson Dissolution System Drivers Operation Manual 81-200-807-D

2.2.5 Vision G2 AutoPlus/Agilent/Non-Hanson Bath Configuration

This configuration includes one, two, or three Non-Hanson Dissolution Test Stations
connected to the Vision G2 AutoPlus and the Agilent 8453 Spectrophotometer. This
configuration will use only the sampler driver. Control of the baths will require a driver from
the bath vendor or other source, if available. See Figure 2-5 for an example setup of this
configuration.

Figure 2-5: Vision G2 AutoPlus/Non-Hanson Bath/Agilent Configuration

2.2.6 Vision G2 Elite 8/Agilent/Non-Hanson Sampling System Configuration

This configuration includes one, two, three, or four Vision G2 Elite 8 Dissolution Test Stations
connected to an Agilent 8453 Spectrophotometer with a non-AutoPlus sampling system such
as the Agilent peristaltic pump/valve system. This configuration will use only the tester driver.
Control of the sampling system will require a driver from the vendor, if available, for the
sampling system or, in the case of the Agilent peri-pump/valve system, the control is
incorporated into the Agilent ChemStation software. See Figure 2-6 for an example setup of
this configuration.

Figure 2-6: Vision G2 AutoPlus/Non-Hanson Bath/Agilent Configuration

Section 2 Introduction –5–

Hanson Dissolution System Drivers Operation Manual 81-200-807-D

3 Specifications
3.1 Software Requirements
Hanson Research recommends using the most current, validated version of the software
available. Dissolution Testing Software must be installed prior to installing the Hanson
Dissolution System Drivers.

 Agilent ChemStation General Purpose Software, p/n G1115AA Version B.04.02 or

higher.

 Agilent ChemStation Advanced Software, p/n G1116AA.

 Agilent Dissolution Testing Software, p/n G1118AA

 Hanson Dissolution System Drivers, p/n 81-200-026 Version 5.00 or higher.

3.2 Hardware Requirements

 Agilent 8453 Spectrophotometer, with a suitable computer that meets or exceeds the
requirements to run the ChemStation program, and has sufficient memory for
dissolution systems and a printer. See the Agilent website for current requirements.

 Agilent 8-Position MultiCell Transport, G1120A (p/n 60-200-031).

 8 Flow cells – 1mm (p/n 91-215-033), 2mm (p/n 91-215-034), 10mm (p/n 91-215-
035), or other sizes by special order, upon request.

 Flow cell tubing kit for 8 cells (p/n 60-200-484).

 Sampling probes, based on system configuration.

 SR8-Plus Firmware Version 0.98 or higher.2

 AutoPlus Firmware Version 1.01 or higher.3

 Vision G2 Elite 8 Tester Firmware Version 2.10 or higher.4

2
Order p/n 73-100-722 to upgrade SR8-Plus Firmware.
3
Order p/n 60-200-724 to upgrade AutoPlus Firmware.
4
Consult Hanson Research for the part number of the Vision Tester Firmware upgrade kit.

Section 3 Specifications –6–

Hanson Dissolution System Drivers Operation Manual 81-200-807-D

 Vision G2 AutoPlus Autosampler Firmware Version 2.11 or higher. 5

3.3 Optional Accessories

 AutoPlus MultiFill™ Collector (p/n 60-200-490) for sample collection with Legacy
instruments.

 Vsion AutoFill™ Collector (p/n 61-201-001) for sample collection with Vision
instruments

 SR8-Plus E-Probe™ Actuator Kit (p/n 73-105-108) for automatic probe actuation.

 Vision G2 Elite 8 Tester Magazine (p/n 74-107-010) for automatic probe actuation for
1 liter vessels.

 Other accessories based on system configuration and intended use.

5
Consult Hanson Research for the part number of the Vision Sampler Firmware upgrade kit.

Section 3 Specifications –7–

Hanson Dissolution System Drivers Operation Manual 81-200-807-D

4 Installation
4.1 Before You Begin
It is important that the software and hardware requirements described in Section 3
(Specifications) are met before beginning the installation.

Note: If the Agilent Security Pack (G1813AA) is to be installed, the installation

and configuration of the security program must be completed prior to installing
the Hanson Research Drivers.

Ensure that each of the Hanson Research instruments that will be used in the system are
properly installed according to the operation manual for each instrument and considering the
final overall setup of the system. Ensure that the Agilent 8453 Spectrophotometer and
corresponding PC workstation are properly installed according to the installation manual
provided by Agilent, again considering the final overall setup of the system. Refer to Figure 4-
1a and 4-1b for suggested placement of the individual instruments on the laboratory bench.

Figure 4-1a Suggested Legacy Instrument Placement on Laboratory Bench

Section 5 Operation and Programming –8–

Hanson Dissolution System Drivers Operation Manual 81-200-807-D

Vision
90 AutoPlus
Elite 8 HP8453 & AutoFill Elite 8 Elite 8

60
ChemStation

30

0
0 30 60 90 120 150 180 210 240 270 300 330 360

(DISTANCES IN CENTIMETERS)

Figure 4-1b Suggested Vision Instrument Placement on Laboratory Bench

Note: Placement of individual instruments will vary based on the actual system
components used.

Section 5 Operation and Programming –9–

Hanson Dissolution System Drivers Operation Manual 81-200-807-D

4.2 Hardware Installation

4.2.1 SR8-Plus/AutoPlus/Agilent Configuration

This configuration below includes one, two, or three SR8-Plus Dissolution Test Stations
connected to the AutoPlus and the Agilent 8453 Spectrophotometer. Refer to Figure 4-2 for
communication connections.

B2 B1 B3

Figure 4-2 Communication Connections for SR8-Plus/AutoPlus/Agilent

Configuration

1. Connect the communication connector, p/n 47-200-373, to the serial port on the
ChemStation PC workstation. Connect the communication cable, p/n 47-200-759, to the
communication connector and to Comm Port 1 on the AutoPlus.

2. Connect communication cable, p/n 47-200-759, from Comm Port 2 on the AutoPlus to
Comm Port 1 on the SR8-Plus that is Bath 1 (B1).

When additional baths are included in the system, verify or make the following connections
as appropriate.

3. If a second bath is included in the system, connect Bath 2 by connecting a

communication cable, p/n 47-200-759, to Comm Port 2 on Bath 1 and Comm Port 1 on
Bath 2.

Section 5 Operation and Programming – 10 –

Hanson Dissolution System Drivers Operation Manual 81-200-807-D

4. If a third bath is included in the system, connect Bath 3 by connecting a communication

cable, p/n 47-200-759, to Comm Port 2 on Bath 2 and Comm Port 1 on Bath 3.

5. If not already connected, connect the MultiFill Collector to Comm Port 3 of the AutoPlus.

Make or verify the following liquid connections. Refer to Figure 4-3 for liquid connections.

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BACD a

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stu v yz s v yz s vwx y

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SR8-Plus B1 AutoPlus MultiFill B3

B2 SR8-Plus HP8453 SR8-Plus

Figure 4-3: Liquid Connections for SR8-Plus/AutoPlus/Agilent Configuration

1. Connect the Source A and B lines to SR8-Plus Bath 1 and Bath 2, as appropriate.

2. Connect the Source C lines to SR8-Plus Bath 3 or media, as appropriate.

3. Connect the AutoPlus D lines to the appropriate lines from the Flow Cell Tubing Kit (p/n
60-200-484), matching color to color for the 8 lines.

4. Connect the remaining lines from the Flow Cell Tubing Kit to the 8 flow cells (color to
color).

Note: Ensure that the direction of the flow is such that the liquid will be following
the correct path into the flow cell as designated by the directional arrow on the
flow cell itself.

Section 5 Operation and Programming – 11 –

Hanson Dissolution System Drivers Operation Manual 81-200-807-D

5. Connect the liquid lines coming out of the flow cells to waste or to the input lines on the
MultiFill Collector, if included in the system.

6. Position the flow cells in the MultiCell Transport on the Agilent 8453.

Note: Hanson Research recommends that the cell in the first position be
connected to the tubing with the clear colored fittings and that the sampling probe
with the clear colored fitting be placed in blank media. It is also recommended
that the cell in the last position be connected to the tubing with the purple colored
fitting and that the sampling probe with the purple fitting be placed in the control
solution, if applicable.

4.2.2 AutoPlus/Agilent/Non-Hanson Bath Configuration

This configuration includes one, two, or three non-Hanson Dissolution Test Stations
connected to the AutoPlus and the Agilent 8453 Spectrophotometer. See Figure 4-4 for
communication connections.

Figure 4-4 Communication Connections for

AutoPlus/Agilent/Non-Hanson Bath Configuration

1. Connect the communication connector, p/n 47-200-373, to the serial port on the
ChemStation PC workstation.

Section 5 Operation and Programming – 12 –

Hanson Dissolution System Drivers Operation Manual 81-200-807-D

2. Connect the communication cable, p/n 47-200-759, to the communication connector and
to Port 1 on the AutoPlus.

3. If not already connected, connect the MultiFill Collector to Port 3 of the AutoPlus.

Make or verify the following liquid connections. Refer to Figure 4-5 for liquid connections.

Figure 4-5 Liquid Connections for AutoPlus/Agilent/Non-Hanson Bath

Configuration

4. Connect the Source A and B lines to Bath 1 and Bath 2, as appropriate.

5. Connect the Source C lines to Bath 3 or media, as appropriate.

6. Connect the AutoPlus D lines to the appropriate lines from the Flow Cell Tubing Kit (p/n
60-200-484), matching color to color for the 8 lines, and connecting one cell to each of
the 8 D lines from the AutoPlus.

Note: Ensure that the direction of the flow is such that the liquid will be following
the correct path into the flow cell as designated by the directional arrow on the
flow cell itself.

Section 5 Operation and Programming – 13 –

Hanson Dissolution System Drivers Operation Manual 81-200-807-D

7. Connect the remaining lines from the Flow Cell Tubing Kit to the 8 flow cells (color to
color).

8. Connect the liquid lines coming out of the flow cells to waste or to the input lines on the
MultiFill Collector, if included in the system.

9. Position the flow cells in the MultiCell Transport on the Agilent 8453.

Note: Hanson Research recommends that the cell in the first position be
connected to the tubing with the clear colored fittings and that the sampling probe
with the clear colored fitting be placed in blank media. It is also recommended
that the cell in the last position be connected to the tubing with the purple colored
fitting and that the sampling probe with the purple fitting be placed in the control
solution, if applicable.

Section 5 Operation and Programming – 14 –

Hanson Dissolution System Drivers Operation Manual 81-200-807-D

4.2.3 SR8-Plus/Agilent/Non-AutoPlus Sampling System Configuration

This configuration includes one, two, three, or four SR8-Plus Dissolution Test Stations
connected to an Agilent 8453 Spectrophotometer with a non-AutoPlus sampling system such
as the Agilent peri-pump/valve system. Only a single Vision Bath may be connected to non-
Hanson samplers. See Figure 4-6 for SR8-Plus communication connections.

Hanson Bath

Figure 4-6 Communication Connections for

SR8-Plus/Agilent/Non-AutoPlus Sampling System Configuration

1. Connect the communication connector, p/n 47-200-373, to the serial port on the
ChemStation PC workstation.

2. Connect the communication cable, p/n 47-200-759, to the communication connector and
to Port 1 on the SR8-Plus that is Bath 1.

When additional SR8-Plus baths are included in the system, verify or make the following
connections as appropriate.

3. If a second bath is included in the system, connect Bath 2 by connecting a

communication cable, 47-200-759, to Port 2 on Bath 1 and Port 1 on Bath 2.

Section 5 Operation and Programming – 15 –

Hanson Dissolution System Drivers Operation Manual 81-200-807-D

4. If a third bath is included in the system, connect Bath 3 by connecting a communication

cable, 47-200-759, to Port 2 on Bath 2 and Port 1 on Bath 3.

5. If a fourth bath is included in the system, connect Bath 4 by connecting a communication

cable, 47-200-759, to Port 2 on Bath 3 and Port 1 on Bath 4.

6. Make or verify the liquid connections according to the installation instructions for the
sampling system installed.

4.2.4 Vision G2 AutoPlus/Vision G2 Elite 8/ Agilent Configuration

This configuration below includes one, two, or three Vision G2 Elite 8 Dissolution Test
Stations connected to a Vision G2 AutoPlus and the Agilent 8453 Spectrophotometer. Refer
to Figure 4-7 for communication connections.

LAN LAN
Network
Switch
LAN LAN
LAN

Elite 8 Elite 8 Vision HP8453 Elite 8 ChemStation

AutoPlus ChemStation
& AutoFill

Figure 4-7 Communication Connections for Vision G2 AutoPlus/ Vision G2 Elite

8 Testers/Agilent Configuration

1. Connect all dissolution instruments, the HP8453 and the Agilent ChemStation computer
to the network switch using network cable p/n 91-211-036.

2. The configuration shown in figure 4-7 is typical but not unique. Depending on
preferences or lab requirements, other configurations are acceptable as long as the
dissolution instruments are in the local area network of the ChemStation computer.

Section 5 Operation and Programming – 16 –

Hanson Dissolution System Drivers Operation Manual 81-200-807-D

3. If not already connected, connect the Vision AutoFill™ Collector to the port labeled
“Collector” Port of the Vision G2 AutoPlus using RS232 cable p/n 47-200-759 .

Make or verify the following liquid connections. Refer to Figure 4-8 for liquid connections.

ABCD

Elite 8 Elite 8 Vision HP8453 Elite 8

AutoPlus
& AutoFill

Figure 4-8: Liquid Connections for Vision G2 Elite 8/Vision G2 AutoPlus/

Agilent Configuration

4. Connect the Source A and B lines to Vision Testers 1 and 2, as appropriate.

5. Connect the Source C lines to a third Vision Tester or media, as appropriate.

6. Connect the Vision AutoPlus D lines to the appropriate lines from the Flow Cell Tubing
Kit (p/n 60-200-484), matching color to color for the 8 lines.

7. Connect the remaining lines from the Flow Cell Tubing Kit to the 8 flow cells (color to
color).

Note: Ensure that the direction of the flow is such that the liquid will be following
the correct path into the flow cell as designated by the directional arrow on the
flow cell itself.

Section 5 Operation and Programming – 17 –

Hanson Dissolution System Drivers Operation Manual 81-200-807-D

8. Connect the liquid lines coming out of the flow cells to waste or to the input lines on the
AutoFill Collector, if included in the system.

9. Position the flow cells in the MultiCell Transport on the Agilent 8453.

Note: Hanson Research recommends that the cell in the first position be
connected to the tubing with the clear colored fittings and that the sampling probe
with the clear colored fitting be placed in blank media. It is also recommended
that the cell in the last position be connected to the tubing with the purple colored
fitting and that the sampling probe with the purple fitting be placed in the control
solution, if applicable.

4.2.5 Vision G2 AutoPlus/Agilent/Non-Hanson Bath Configuration

This configuration includes one, two, or three non-Hanson Dissolution Test Stations
connected to the Vision G2 AutoPlus and the Agilent 8453 Spectrophotometer. See Figure 4-
9 for communication connections.

LAN
Network
Switch
LAN

GENERIC BATH Vision HP8453 ChemStation

AutoPlus
GENERIC BATH & AutoFill GENERIC BATH

Figure 4-9 Communication Connections for Vision G2 AutoPlus/Agilent/

Non-Hanson Bath Configuration

1. Connect the Vision G2 AutoPlus Autosampler, the HP8453 and the Agilent ChemStation
computer to the network switch using network cable p/n 91-211-036.

Section 5 Operation and Programming – 18 –

Hanson Dissolution System Drivers Operation Manual 81-200-807-D

2. The configuration shown in figure 4-9 is typical but not unique. Depending on
preferences or lab requirements, other configurations are acceptable as long as the
Vision G2 AutoPlus in the local area network of the ChemStation computer.

3. If not already connected, connect the Vision AutoFill™ Collector to the port labeled
“Collector” Port of the Vision G2 AutoPlus using RS232 cable p/n 47-200-759 .

Make or verify the following liquid connections. Refer to Figure 4-10 for liquid connections.

ABCD

GENERIC GENERIC Vision HP8453 GENERIC

BATH BATH AutoPlus BATH
& AutoFill

Figure 4-10 Liquid Connections for Vision G2 AutoPlus/Agilent/

Non-Hanson Bath Configuration

4. Connect the Source A and B lines to Bath 1 and Bath 2, as appropriate.

5. Connect the Source C lines to Bath 3 or media, as appropriate.

6. Connect the AutoPlus D lines to the appropriate lines from the Flow Cell Tubing Kit (p/n
60-200-484), matching color to color for the 8 lines, and connecting one cell to each of
the 8 D lines from the Vision G2 AutoPlus.

Section 5 Operation and Programming – 19 –

Hanson Dissolution System Drivers Operation Manual 81-200-807-D

Note: Ensure that the direction of the flow is such that the liquid will be following
the correct path into the flow cell as designated by the directional arrow on the
flow cell itself.

7. Connect the remaining lines from the Flow Cell Tubing Kit to the 8 flow cells (color to
color).

8. Connect the liquid lines coming out of the flow cells to waste or to the input lines on the
AutoFill Collector, if included in the system.

9. Position the flow cells in the MultiCell Transport on the Agilent 8453.

Note: Hanson Research recommends that the cell in the first position be
connected to the tubing with the clear colored fittings and that the sampling probe
with the clear colored fitting be placed in blank media. It is also recommended
that the cell in the last position be connected to the tubing with the purple colored
fitting and that the sampling probe with the purple fitting be placed in the control
solution, if applicable.

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4.2.6 Vision Elite 8/Agilent/Non-AutoPlus Sampling System Configuration

This configuration includes one, two, three, or four Vision G2 Elite 8 Dissolution Test Stations
connected to an Agilent 8453 Spectrophotometer with a non-AutoPlus sampling system such
as the Agilent peri-pump/valve system. See Figure 4-11 for communication connections.

LAN LAN
Network
Switch
LAN LAN
LAN

Elite 8 Elite 8 ChemStation HP8453 Elite 8 Hanson Bath

Elite 8

GENERIC SAMPLER

Figure 4-11 Communication Connions for Vision G2 Elite 8 Tester/

Agilent/Non-AutoPlus Sampling System Configuration

1. Connect all dissolution testers, the HP8453 and the Agilent ChemStation computer to
the network switch using network cable p/n 91-211-036.

2. The configuration shown in figure 4-11 is typical but not unique. Depending on
preferences or lab requirements, other configurations are acceptable as long as the
dissolution instruments are in the local area network of the ChemStation computer.

3. Make or verify the liquid connections according to the installation instructions for the
sampling system installed.

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4.3 Software Installation

The Hanson Dissolution System Drivers CD (p/n 81-200-026) contains two drivers—one for
the SR8-Plus and Vision G2 Elite 8 Dissolution Test Stations and another for the Legacy
AutoPlus and the Vision G2 AutoPlus Autosampler. If your system contains an AutoPlus
sampler and one or more Hanson baths, both drivers will be installed and will work together
with the ChemStation. If your system contains only Hanson baths, then the bath driver alone
will be installed and work in conjunction with your peristaltic pump and valve systems or other
sampling system. The bath driver will work independently of drivers for autosampler systems
other than the Agilent peristaltic pump and valve system. If your system contains an AutoPlus
and non-Hanson baths, the AutoPlus driver will be installed and work independently of the
other manufacturer’s bath driver.

4.3.1 Uninstalling Previous Installations of the Hanson Dissolution System Drivers

If the Hanson Dissolution System Drivers have been previously installed on the ChemStation
computer, make sure that you uninstall any previous installation (even of the same revision)
of the dissolution drivers before attempting an installation.

To uninstall a previously installed version of the Hanson Dissolution System Drivers:

1. Open the ChemStation program and switch to Dissolution Testing Mode

(ModeDissolution Testing Mode).

2. If necessary, select the On-line Hanson AutoPlus from the Sampling menu
(InstrumentSelect Sampling SystemOn-line Hanson AutoPlus) to activate the
AutoPlus icon.

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3. Click on the AutoPlus icon and select the Options menu.

The following dialog box will appear.

4. Click on the Remove AutoPlus Driver button. This will remove the AutoPlus driver from
the ChemStation configuration register.

5. Click Yes at “Do you really want to remove the AutoPlus Driver?” prompt and OK at the
“You must restart ChemStation for changes to apply.”

6. Click OK to close the Hanson AutoPlus Options window.

7. Exit ChemStation.

8. Click on the Windows Start button and select Programs | Hanson Research | Uninstall.

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This will uninstall the Hanson Research Drivers from the system.

9. Restart ChemStation and verify that the AutoPlus is removed from the list of available
sampling systems.

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10. Additionally, verify that when Dissolution Bath Status is selected from the Instrument
menu, no Dissolution Bath Status window opens.

4.3.2 Installing the Hanson Dissolution Drivers

Before beginning the driver installation verify that the AutoPlus and SR8-Plus or Vision
Dissolution Test Stations have their power turned on and that they are properly connected as
described in Section 4.2 (Hardware Installation).

1. The Hanson Dissolution Drivers are provided on a CD ROM. Begin the installation by
inserting the disc and selecting Install Drivers from the CD menu.

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3. The following splash screen is displayed once the installation process initializes, click on the
Next button to continue.

4. Follow the on-screen instructions from the InstallShield Wizard. Enter a user name or
department name in the Name Field. Enter the company name in the Company Field. Enter
the serial number from the serial number label on the CD ROM case in the Serial Field.

5. Select the path to the ChemStation directory as the Destination Directory. Typically, the
path will be C:\hpchem if upgrading from a previous version of ChemStation, or

Section 5 Operation and Programming – 26 –

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C:\Chem32 for a new installation. These are the default ChemStation directories. If
necessary, use the Change button to modify the path.

6. Choose Next to create and add program icons to the Hanson Research program folder.

The InstallShield Wizard will continue the installation and configure the drivers required
by your system configuration.

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7. Once the setup program is finished copying the required files to the proper locations,
Restart the computer in order for Windows to load the new files properly.

8. Run the ChemStation program and select the Dissolution Testing Mode.

Once you select the Dissolution Testing mode, the setup program will configure the
ChemStation to recognize the new drivers added.

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9. If your system contains legacy instruments and they are connected properly and
powered, the configuration editor will detect your configuration automatically. A window
similar to the one below is displayed depending on your configuration:

10. If your system contains Vision instruments which require a static I.P address (usually
obtained from the I.T. department of the lab), you need to select the type of instrument
and enter its UNIQUE address.

11. Click on the Test Settings button and wait until the green checkmarks are displayed, if
not check the configuration settings and connections and click on Test Settings again.

12. Once you have green check marks on all the instruments selected, click Save and Exit
and Restart ChemStation in order for the changes to take effect.

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5 Operation and Programming

This section will discuss only those operation and programming features that may differ from
the operation and programming features defined by Agilent and discussed in the manuals
provided with the ChemStation software.

5.1 Dissolution Driver

Start the ChemStation and select either Dissolution Testing Mode or Multibath Dissolution
Testing Mode, as desired.

The ChemStation will load the dissolution bath driver automatically. The driver can be used to
view the status of a Bath 1 in Dissolution Testing Mode or of each configured bath in
Multibath Dissolution Testing Mode, as follows.

To view the status of Bath 1 in Dissolution Testing Mode, select Dissolution Bath Status from
the Instrument menu.

To view the status of any configured bath in Multibath Dissolution Testing Mode, first select
the desired bath from the available baths.

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With the desired bath selected, select Dissolution Bath Status from the Instrument menu.

In Dissolution Testing Mode a window will open for Bath 1. In Multbath Dissolution Testing
Mode a window will open for the selected bath.

Section 5 Operation and Programming – 31 –

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5.1.1 Test Station Tab

Status
Resembles the SR8-Plus display and allows the user to monitor the test station status of the
selected bath.

Bath Parameters
Allows the user to manually control the stirrer and temperature control of the bath and to
verify that ChemStation is communicating with the bath.

E-Probes
Allows the user to control the SR8-Plus E-Probes, Auto-Probes (for the Vision G2 Elite 8
Auto-Mag), or other automated sampling probes.

Note: The E-Probes frame will be grayed out if this feature is not available or is
disabled in your system.

Handling of Bath Parameters

Allows the user to specify whether or not bath parameters are implemented immediately upon
loading the method. Normally you would want Change stirrer settings immediately
unchecked and Change heater settings immediately checked so that when you load a
method, the motor does not start but the bath starts to heat to the target temperature.

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Hide Details
Allows the user to hide all of the details of the bath driver window and show only the status
display. The display floats above all other windows to allow constant bath monitoring.

Print
Allows the user to print the bath status information to the ChemStation printer.

5.1.2 Reference Tab

Reference Serial Numbers

Allows the user to keep track of the vessel, paddle, basket, and basket shaft serial numbers
used with the test station.

Hide Details
Allows the user to hide all of the details of the bath driver window and show only the status
display. The display floats above all other windows to allow constant bath monitoring.

Print
Allows the user to print the reference information to the ChemStation printer.

Section 5 Operation and Programming – 33 –

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5.1.3 Setup Tab

Note: Typically, the settings in this tab will not be changed. Contact Hanson
Research before making changes to these settings.

Tester Model
Either SR8-Plus or Vision G2 Dissolution Testers.

IP Address or RS232 Serial Port

The serial port where the tester is connected or the static I.P. address of the Vision tester.

Instrument Identification
Edit the identification string that the bath uses to respond to remote commands.

Log Level
Creates a communications log to aid in troubleshooting.

Test Setup
The serial port where the tester is connected or the static I.P. address of the Vision tester.

Hide Details
Hide extra details of status window and show only the status display. The display floats
above all other windows to allow constant bath monitoring.

Print
Allows the user to print the setup information to the ChemStation printer.

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5.1.4 About Tab

The about tab contains important information about the dissolution bath driver such as the
part number, revision, company, user, and serial number.

Note: Always have the information displayed on the About Tab ready when contacting
Hanson Technical Support.

Print
Allows the user to print the setup information to the ChemStation printer.

Close
Hide status window completely.

Section 5 Operation and Programming – 35 –

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5.2 AutoPlus Driver

To access the functions of the Hanson AutoPlus (or Vision-Enabled AutoPlus) Driver, click on
the AutoPlus icon (which looks like a light blue circle with a syringe in front). The functions
available to the user are described in this section.

Transfer
The action of moving a volume of fluid from a source to a target location. This function
operates like the Transfer function in the AutoPlus Automatic Syringe Control. To use this
function, enter the desired transfer volume and select a Source (A-D) and a Target (A-D).
Click the Transfer button to perform the transfer.

Whenever the Blank, Standard, or Sample spectrophotometer functions are selected, the
driver will instruct the AutoPlus to pump liquid through the flow cells at the volume selected in
the Transfer function. To disable this action, disable Auto Rinse/Wait as indicated in the Auto
Rinse/Wait description on the next page.

Wash
The action of clearing out the liquid from the sampling line using blank or wash media. This
function operates like the Wash function in the AutoPlus Automatic Syringe Control.

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To use this function enter the desired wash volume and select a Source (A-D) and a Target
(A-D or All). Click the Wash button to perform the wash. Use this feature as dictated by your
prerun or postrun requirements.

Prime
The action of purging air from the sampling line with fluid. This function operates like the
Prime function in the AutoPlus Automatic Syringe Control.

To use this function, enter a prime volume and select a Source (A-D or All) and a Target (A-D
or All). Click the Prime button to perform the prime. Use this feature as dictated by your
prerun or postrun requirements.

Note: The user must ensure that the media replace line is primed or purged of
air before starting a sampling procedure that requires media replacement.

Auto Rinse/Wait
Clicking on this menu item either enables (checked) or disables (unchecked) this option. If
this option is enabled, the driver will instruct the AutoPlus to pump the volume specified in the
Transfer function whenever the user chooses to measure a blank, standard, or sample from
the buttons in ChemStation or the corresponding buttons on the 8453. When using these
functions, the AutoPlus will not pump if this option is disabled.

Section 5 Operation and Programming – 37 –

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Test Parameters
Allows the user to rinse the flow cells with a certain volume before each measurement, or to
select an AutoPlus protocol (Legacy by number, Vision by Name) whenever the ChemStation
initiates a sampling event (measurement cycle). Select a wait time that allows the flow cell to
be full and stable before the ChemStation starts reading the flow cells. The AutoPlus Test
Parameters can also be accessed from the Instrument menu by selecting Setup Sampling
System (which brings up the Setup MultiCell Transport dialog box) and clicking the
Parameters button.

Options
Opens the following window of options.

Typically, the “Position cell changer…” option will be selected (checked). With this option
selected, the cell changer will automatically move to the cell position that is designated as the
blank in the Measure Cycle Definition when the blank is measured manually; when the
standard is measured manually, the cell changer will automatically move to the position
designated as the control in the Measure Cycle Definition. When this option is not selected
(unchecked), the cell changer will remain in its current position when the blank and standard
are measured manually.

Section 5 Operation and Programming – 38 –

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Clicking the Remove AutoPlus Driver button will remove the AutoPlus driver from the
ChemStation registry. Removal of the AutoPlus Driver cannot be undone. If the AutoPlus
Driver is removed from the ChemStation registry, the Hanson Dissolution Drivers must be
completely uninstalled as per Section 4.3.1 and reinstalled as per Section 4.4.

Log Level
Creates a communications log to aid in troubleshooting.

Help
Gives the user access to the AutoPlus Driver Help system.

About
The About screen contains important information about the dissolution bath driver such as
the part number, revision, company, user, and serial number.

Note: Always have the information displayed on the About screen ready when
contacting Hanson Technical Support.

Section 5 Operation and Programming – 39 –

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Creating a ChemStation Method

5.2.1 Creating an AutoPlus Protocol (Optional)

If the AutoPlus is used on the system, you have the option of having the ChemStation
program instruct the AutoPlus to run an AutoPlus protocol at each sampling time point. This
option is required when using AutoPlus Collect and Detect methods and when using the
Retrieval Reservoir.

At a minimum, the AutoPlus protocol must be set up to rinse and detect. Additional options
can be programmed for collection and retrieval as needed. Refer to the AutoPlus Operation
Manual (for Legacy p/n 60-200-802, for Vision p/n 61-108-010 ) for protocol programming.
For the Legacy AutoPlus, only one sampling point is required as the AutoPlus protocol will be
run at each sampling point. For the Vision G2 AutoPlus, no sampling points are required.

5.2.2 Creating a ChemStation Dissolution Method

1. Open the Edit Method window by clicking the Setup button in the Dissolution window.

2. Choose Product/Bath, or . . .

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. . . choose Edit Product Info and Bath Parameter from the Method menu.

This will open the Product, Bath and Info Method Parameters window.

3. Complete the information on the Product tab screen according to your method needs
and the requirements for the ChemStation system.

4. Complete the information on the Bath tab screen according to your method needs and
the requirements for the ChemStation system.

Section 5 Operation and Programming – 41 –

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5. Click on the Advanced button to setup the interval at which the speed and temperature
status data is logged. If so equiped, choices appear to actuate the automatic E-Probe
control , to set the default stroke length which controls the travel of the e-probe or
magazine and the pill dropper use.

6. Complete the information on the Options & Info tab screen according to your method
needs and the requirements for the ChemStation system.

7. If the Hanson Research SR8-Plus or Vision Driver is installed, the bath parameters are
to be controlled by the ChemStation. Place a check in the Bath Parameters are
controlled by ChemStation box by clicking in the box. This will allow the ChemStation

Section 5 Operation and Programming – 42 –

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program to control the start and stop of the stirrers, the stir speed, and the bath
temperature.

Note: When a “new” method is created, the ChemStation defaults to “Bath

Parameters are controlled by ChemStation unchecked!

8. From the Edit Method window click Evaluation, or . . .

. . . choose Edit Evaluation Parameter from the Method menu to open the Evaluation
Method Parameters window.

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9. Complete the information on the Analysis tab screen according to your method needs
and the requirements for the ChemStation system.

10. Complete the information on the Calculation tab screen according to your method
needs and the requirements for the ChemStation system.

Section 5 Operation and Programming – 44 –

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11. Complete the information on the QC Limits tab screen according to your method needs
and the requirements for the ChemStation system.

12. From the Edit Method window click Diss.-Run, or . . .

. . . choose Edit Dissolution Run Parameter from the Method menu to open the
Dissolution Run Method Parameters window.

Section 5 Operation and Programming – 45 –

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13. Complete the information on the PreRun Seq. tab screen according to your method needs
and the requirements for the ChemStation system. Refer to Appendix A for details on the
Actions and Parameters that are available when utilizing the Hanson Research Drivers.

14. Complete the information on the Dissolution Run tab screen according to your method
needs and the requirements for the ChemStation system. Refer to Appendix A for details
on the Actions and Parameters that are available when utilizing the Hanson Research
Drivers.

Section 5 Operation and Programming – 46 –

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15. Complete the information on the PostRun Seq. tab screen according to your method
needs and the requirements for the ChemStation system. Refer to Appendix A for
details on the Actions and Parameters that are available when utilizing the Hanson
Research Drivers.

Section 5 Operation and Programming – 47 –

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5.2.3 Defining the Measurement Cycle

When running dissolution tests utilizing the Hanson AutoPlus and the Agilent MultiCell
Transport for the 8453, it is necessary to identify the intended contents of each UV cell in the
MultiCell Transport (i.e. Blank, Sample, Control, or Not Used).

To define the measurement cycle,:

1. Choose Define Measurement Cycle from the Method menu to open the Measure Cycle
Definition window.

The Total Steps per Measure Cycle is always 8 when using the 8-position MultiCell
Transport, regardless of how many cells will actually be used.

2. Using the drop down menu for each cell position (Meas. No), set the Measure As to
Blank, Sample, Control, or Not Used, as appropriate for the test method.

Section 5 Operation and Programming – 48 –

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Note: Agilent and Hanson Research recommend that the first cell (Meas. No 1)
always be set to Blank. Hanson Research recommends that the AutoPlus tubing
from the AutoPlus syringe position number 7 (clear) be directed to this cell and
that the sample probe be directed to a container of blank media.

3. Enter the appropriate drawn Volume and added Volume as required by your method to
automatically calculate the volume change corrections.

5.2.4 Defining the Control (Optional)

1. If a control is being used, choose Define Control for Measurement Cycle from the
Method menu to open the Control for Measurement Cycle Definition window.

2. Enter the Analyte Name, Concentration, Unit, and Dev. Limit % as appropriate for the
test method.

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5.2.5 Setting Up the Sampling System

1. With the On-line Hanson AutoPlus selected as the sampling system, click the Setup
button in the Sampling window, or . . .

. . . choose Setup Sampling System from the Instrument menu to open the Setup
Multicell Transport window.

2. Enter the Cell Path Length for each cell as appropriate for the test method.

Section 5 Operation and Programming – 50 –

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3. Click the Parameter button to open the Hanson AutoPlus Test Parameters window.

4. Choose Rinse flowcells with or Run AutoPlus protocol #.

When Rinse flowcells with is selected, ChemStation will instruct the AutoPlus to pump
the specified volume from the source dissolution bath through the flow cells at each
sampling time point. The Wait before reading flowcells for time automatically defaults
to 10 seconds of wait per 1 mL of volume pumped. This is the recommended setting.

When Run AutoPlus protocol is selected, it is necessary to choose the appropriate

AutoPlus protocol from the drop-down list. The protocol is selected by number (1-25) for
the Legacy AutoPlus and by name for the Vision G2 AutoSampler (as shown below).
The ChemStation will instruct the AutoPlus to run the selected protocol at each sampling
time point. This option is required for Collect and Detect methods and whenever the
Retrieval Reservoir is used.

5. Click the Print button (if desired) in the Hanson AutoPlus Test Parameters window to
print the AutoPlus test parameters that will be used with the ChemStation.

Section 5 Operation and Programming – 51 –

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6 Running a Test and Reviewing Data

The information in this section is intended to be a general overview on running a dissolution
test. For more complete information, refer to the ChemStation reference materials and
Section 5 (Operation and Programming) of this operation manual.

1. Power on the 8453, the SR8-Plus or Vision Dissolution Test Station(s), the AutoPlus,
and the MultiFill collector, if used.

2. Launch the ChemStation program and turn on the appropriate lamp(s)

(InstrumentLamps).

3. Choose Dissolution Testing Mode (single bath) or Multibath Dissolution Testing

Mode as appropriate for the configuration of the system.

6.1 Zero the Cells (if done previously, go to Section 6.2)

Note: Zeroing the cells is only required if the cells are moved from their positions or
replaced AFTER zero cells is performed. It does not need to be repeated otherwise.

When using the MultiCell Transport, it is appropriate to match the cells by using the Zero
Cells option. Agilent recommends that the cells be filled with purified water to zero the cells.
During the zeroing process, the cell in Position 1 will be set to zero at all wavelengths. Each
subsequent cell is evaluated against the first cell, and values are obtained and stored in the
zero cells registry to correct for the cell variations at each wavelength.

The cells should be zeroed whenever the cells are moved from their positions in the MultiCell
Transport or if the cells are changed. If, when attempting to run a ChemStation method, a
message appears indicating that there are no zero cell values, it is recommended that the
cells be zeroed before continuing with the test.

Section 6 Running a Test and Reviewing Data – 52 –

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1. In Dissolution Testing mode (single bath mode), fill the cells with water by using the
Prime function described in Section 5 (Operation and Programming) or by using the
AutoPlus syringe controls.

2. Choose Zero Cells from the Hanson menu.

3. Run and save the zero cells data.

4. In Multibath Dissolution Testing mode, fill the cells with water by using the Prime
function described in Section 5 (Operation and Programming) or by using the AutoPlus
syringe controls.

5. Choose Spectrophotometer Reference from the Measure menu.

The MultiCell Transport will move to the reference position and the measurement will be
made.

6. Choose Zero Cells from the Measure menu.

7. Run and save the zero cells data.

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6.2 Spectrophotometer Reference (Multibath Dissolution

Testing Only)
In order to operate the system in Mutibath mode, it is necessary to perform the
spectrophotometer reference prior to running blanks, standards, or samples. This reference
must be measured each time the ChemStation program is opened and after switching back to
the Multibath mode from any other mode.

To measure the reference:

1. Choose Spectrophotometer Reference from the Measure menu.

The MultiCell Transport will move to the reference position and the measurement will be
made.

2. Load or Create the ChemStation method.

Note: To create a new method, follow the steps in Section 5 (Operation and
Programming) in this manual and the appropriate ChemStation reference information.

3. To load an existing method, choose Load Method from the File menu. An existing
ChemStation method will contain the dissolution setup, the definitions of the
measurement cycle and control, and the sampling system setup.

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The existing method will be loaded and the method name will be displayed in the Method
field.

Note: When running in Multibath Dissolution Testing mode, a method must be

loaded or created for each bath that will be operated.

6.3 Load or Run Standards

In order to analyze the data and calculate dissolution results, standard information must be
available. Standard data may be available in the ChemStation method, if the method was
saved with standard data. Additionally, standard data may be saved in a standard file that
can be loaded.

To load standards from a file:

1. Choose Load Standards from the File menu.

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2. To run new standards prior to beginning the dissolution test, fill the appropriate cell with
standard by using the Transfer function described in Section 5 (Operation and
Programming), by using the AutoPlus syringe controls, or by allowing the AutoPlus to
pump (Auto Rinse/Wait on) with the appropriate volume programmed in the AutoPlus
Transfer feature within ChemStation (see Section 5 [Operation and Programming]).

3. Click the Standard button to initiate the measurement of the standard(s).

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Note: It is possible to run standards as part of the Pre-Run Seq. in the

Dissolution Setup; however, this requires pumping from the AutoPlus through all
eight sampling lines and will require additional pre-run preparation. Refer to the
ChemStation reference materials and Appendix A of this operation manual for
additional information.

4. With the appropriate ChemStation method(s), standards, and other necessary items
loaded for each bath, initiate the dissolution test by clicking the Run Dissolution button.

The test will run according to the parameters programmed in the ChemStation method.

5. At the conclusion of each test, clean up each instrument according to its individual clean
up instructions.

Note: For additional information, refer to the ChemStation reference materials

and the operation manual(s) or user guide(s) for the Vision, SR8-Plus, AutoPlus,
and Retrieval Reservoir, as necessary.

Section 6 Running a Test and Reviewing Data – 57 –

Hanson Dissolution System Drivers Operation Manual 81-200-807-D

7 Troubleshooting
Problem Possible Solution

ChemStation installation qualification Possible corrupt or missing files; reinstall

fails. the ChemStation.

Dissolution testing modes are not Licenses must be purchased and

available. dissolution modules installed.

Installation program fails to install the Communication problem:

dissolution system drivers. – check communication setup and make
sure instruments are on during the
installation.
– reinstall drivers.

ChemStation does not start the SR8-Plus Configuration problem:

or Vision driver(s). – check configuration bath parameters for
proper path to dissolution drivers.

ChemStation does not start the AutoPlus Configuration problem:

driver. – select AutoPlus as sampling system and
save the ChemStation configuration.

Section 7 Troubleshooting – 58 –
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Problem Possible Solution

When a dissolution test starts, speed and Communication problem:

temperature parameters are not sent to – make sure baths are online.
the dissolution bath.
Method setup problem:
– make sure that option “Bath Parameters
are controlled by ChemStation” is enabled
in method.

AutoPlus menu does not appear when Communication problem:

AutoPlus icon is clicked. – AutoPlus is offline.

Drivers display wrong version, part Improper installation:

number, or operation mode. – make sure previous drivers have been
uninstalled and proper versions are
installed.

Section 7 Troubleshooting – 59 –
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8 Maintenance and Calibration

No maintenance or calibration is required on the Hanson Dissolution System Drivers.

Refer to the appropriate operation manuals or other reference materials for the maintenance
and calibration requirements for the individual instruments operating in the system.

Section 8 Maintenance and Calibration – 60 –

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9 Moving and Storage

No moving or storage requirements exist for the Hanson Dissolution System Drivers.

Refer to the appropriate operation manuals or other reference materials for the moving and
storage requirements for the individual instruments operating in the system.

Section 9 Moving and Storage – 61 –

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10 Service Parts
No service parts exist for the Hanson Dissolution System Drivers.

Refer to the appropriate operation manuals or other reference materials for appropriate
service parts for the individual instruments operating in the system.

Section 10 Service Parts – 62 –

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11 Sales and Support

Hanson Research instruments are sold and serviced by a worldwide network of dealers and
distributors. If you need additional accessories, replacement parts, or require service
information, you may either contact your local agent, or you can contact Hanson Research
Corporation at our corporate headquarters:

Hanson Research Corporation

Corporate 9810 Variel Avenue

Headquarters Chatsworth, CA 91311 USA

P: 818.882.7266 or 800.821.8165 (toll free)

F: 818.882.9470

Web Site www.hansonresearch.com

Sales Support For quotes, literature, and sales assistance in the USA
and worldwide, please email:
[email protected].

Customer Support To place an order, obtain an order’s status, or request a

return authorization, please email:
[email protected].

Technical Support For assistance with applications and other technical

support and service questions, please use our online form:
www.hansonresearch.com/tsr.

When contacting Hanson Research, please help us eliminate duplication of effort amongst personnel and
departments by directing your inquiry to one department or representative.

Section 11 Sales and Support – 63 –

Hanson Dissolution System Drivers Operation Manual 81-200-807-D

12 General Warranty
Hanson Research Corporation (Hanson) products are warranted for one full year, including
parts and labor. Service and maintenance contracts are available for post-warranty support.
International dealer warranties may vary. Hanson makes no warranty, expressed or implied,
for glassware, consumables, or products not manufactured by Hanson (as evidenced by
nameplate on the item or other designation). Hanson will give responsible assistance to the
buyer in obtaining from the respective manufacturer whatever adjustment is reasonable in
light of the manufacturer’s own warranty. Hanson shall be released from any and all
obligations under any warranty, either expressed or implied, if the warranted product is
repaired or modified by anyone other than Hanson personnel or an authorized representative,
or if such repair or modification is made without written authorization from Hanson. There are
no other warranties, expressed or implied, and Hanson shall not be liable under any
circumstances for consequential damages.

Section 12 General Warranty – 64 –

Hanson Dissolution System Drivers Operation Manual 81-200-807-D

Appendix A
Dissolution Run Parameters
This Appendix identifies the parameters that can be selected when editing the Dissolution
Run Parameters, and describes how they work within the environment created when the
Hanson Dissolution System Drivers are installed with the ChemStation program. In some
cases, more specific information may be obtained from the ChemStation reference
information than is listed in this Appendix.

PreRun Seq.
The PreRun Seq. tab of the Dissolution Run Method Parameters dialog box contains a table
that allows you to set up a sequence of actions that are performed prior to the start of the
dissolution test. The table contains three columns:

# Column
The # column shows the line number of the sequence of actions. The actions are performed
sequentially from the lowest line number to the highest.

Action
The Action column defines the actions in the sequence.

Parameters
The Parameters field shows the values of the parameters set in the appropriate Setup dialog
box. If no parameters have been specified, the Parameters field displays the message
>>> not set <<<. The requirements for the Parameters field are determined by the action
specified.

The possible actions are defined in a drop-down list, which is displayed by activating the
Action field and selecting the down arrow. These actions are:

 Remote Operation: Does not operate with the Hanson Dissolution System Drivers
installed.

 Operator Message: Pops up a window with a message to the operator.

 Wash Cycle: Does not operate with the Hanson Dissolution System Drivers installed.

Appendix A Dissolution Run Parameters – 65 –

Hanson Dissolution System Drivers Operation Manual 81-200-807-D

 Flow Rate Test: Does not operate with the Hanson Dissolution Drivers installed.

 Medium Test: Checks the purity of the solvent in all vessels by measuring the
absorbance on all channels/vessels. The maximum absorbance and the wavelength
range can be defined by the user. Operating with the Hanson Dissolution System Drivers
installed, this test requires that the cells be pre-filled by using a UserMacro action (such
as hrTransfer) to fill the cells with media from the vessels. This action should be
prioritized to run before the Medium Test action.

 Measure Blank: Measures a blank spectrum. A good blank measurement shows a flat
baseline with A<10-3 AU. Operating with the Hanson Dissolution System Drivers
installed, this action requires that the cell be pre-filled by using a UserMacro action (such
as hrTransfer) to fill the Cell Position 1 with blank media from an appropriate source
before the measurement. This action should be prioritized to run before the Measure
Blank action. Operating with the Hanson Dissolution System Drivers installed, the
Channel is always set to 1. The cell position measured is always Position 1.

 Measure Control: A sample of known concentration used to validate the calibration.

Operating with the Hanson Dissolution System Drivers installed, this action requires that
the cell be pre-filled by using a UserMacro action (such as hrTransfer) to fill the Cell
Position 1 with the control solution from an appropriate source before the measurement.
This action should be prioritized to run before the Measure Control action. Operating with
the Hanson Dissolution System Drivers installed, the Channel is always set to 1. The cell
position measured is always Position 1.

 ReMeasure Standard: Prompts the operator to remeasure the standard used to calculate
the dissolution results. The ChemStation options are: 1) Replace existing Standard: The
“old“ standards will be replaced by the remeasured standards; 2) Average with existing
Standards: The remeasured standards are appended to the existing standard; hence,
they will be averaged.

Operating with the Hanson Dissolution System Drivers installed, this action requires that
the cell be pre-filled by using a UserMacro action (such as hrTransfer) to fill the Cell
Position 1 with the standard solution from an appropriate source before the
measurement. This action should be prioritized to run before the ReMeasure Standard
action. Operating with the Hanson Dissolution System Drivers installed, the Channel is
always set to 1. The cell position measured is always Position 1.

Appendix A Dissolution Run Parameters – 66 –

Hanson Dissolution System Drivers Operation Manual 81-200-807-D

 ReMeasure Capsule Background: Remeasures the Capsule Background spectrum used

for the background correction. Operating with the Hanson Dissolution System Drivers
installed, this action requires that the cell be pre-filled by using a UserMacro action (such
as hrTransfer) to fill the Cell Position 1 with the capsule background solution from an
appropriate source before the measurement. This action should be prioritized to run
before the ReMeasure Capsule Background action. Operating with the Hanson
Dissolution System Drivers installed, the Channel is always set to 1. The cell position
measured is always Position 1.

 ReMeasure Auxiliary: Remeasures the Auxiliary spectrum used for auxiliary correction.
Operating with the Hanson Dissolution System Drivers installed, this action requires that
the cell be pre-filled by using a UserMacro action (such as hrTransfer) to fill the Cell
Position 1 with the capsule background solution from an appropriate source before the
measurement. This action should be prioritized to run before the ReMeasure Auxiliary
action. Operating with the Hanson Dissolution System Drivers installed, the Channel is
always set to 1. The cell position measured is always Position 1.

 Bath Parameters: Immediately changes the bath speed and temperature to those
specified in the parameters.

Caution: The method parameters (MethodEdit Product Info and Bath

ParameterBath) are not changed, and the system will revert to method
parameters at the start of the Dissolution Run.

 Lower/Lift Probes: Moves the probes if automated probes are installed. Lower Probes
and Lift Probes actions must be separated by another action for the actions to be
effective.

 Goto Position: Does not operate with the Hanson Dissolution System Drivers installed.

 Pump: Does not operate with the Hanson Dissolution System Drivers installed.

 User Macro: Executes a user-defined macro. The AutoPlus Driver contains the following
macros which can be called from the User Macro function in the PreRun Sequence:

o hrTransfer (Volume, Source, Dest): Transfers a volume from a source to a

destination. Sources can be A, B, C, D, or X for Current Bath (e.g., to transfer 5.0 mL
from the Current Bath to D, enter hrTransfer 5.0, X, D in the Parameters field).

Appendix A Dissolution Run Parameters – 67 –

Hanson Dissolution System Drivers Operation Manual 81-200-807-D

o hrPrime: Runs the prime function with the parameters selected in the AutoPlus icon.

o hrWash: Runs the wash function with the parameters selected in the AutoPlus icon.

o hrRun (Protocol Number): Runs an AutoPlus protocol, e.g., to run AutoPlus Protocol
3, enter hrRun 3 in the Parameters field.

 Wait: User-defined wait time. Can be used for time-limited actions indicated by an
Operator Message.

Note: Clicking the Check button will perform a consistency check of all actions
that have been setup in the PreRun Sequence, Dissolution Run, and PostRun
Sequence. If any inconsistencies are detected, the Consistency Check Results
window pops up, and shows all Errors (E) and Warnings (W).

Dissolution Run
The Dissolution Run tab of the Dissolution Run Method Parameters dialog box contains a
timetable that allows you to set up a sequence of actions that are performed at specified
times during the dissolution test. The timetable contains three columns:

Min
The Min column shows the time (in minutes from the start of the run) at which the specified
action is set to be performed.

Note: To measure vessels at 0 minutes, Lower Probes must be in the Pre-Run

Seq. of actions.

Action
The Action column defines the actions in the sequence.

Parameters
The Parameters field shows the values of the parameters set in the appropriate Setup dialog
box. If no parameters have been specified, the Parameters field displays the message
>>> not set <<<. The requirements for the Parameters field are determined by the action
specified.

The possible actions are defined in a drop-down list, which is displayed by activating the
Action field and choosing the down arrow. These actions are:

Appendix A Dissolution Run Parameters – 68 –

Hanson Dissolution System Drivers Operation Manual 81-200-807-D

 Remote Operation: Does not operate with the Hanson Dissolution System Drivers
installed.

 Measure Vessels: Measure cycle as defined under MethodDefine Measurement Cycle.

 Pause: Stops the Dissolution Run for a user-defined time (e.g., to change or replace a
certain amount of liquid for a pH change).

 Timed Operator Message: User-defined message to the operator over a user-defined

time.

 Add/Remove/Replace Volume: Do not operate with the Hanson Dissolution System

Drivers installed.

 Zero Vessels: Measure cycle at time t=0 as defined under MethodDefine Measurement
Cycle. The absorbance A0i of Vessel i (i=1…8) at time t=0 is subtracted from all following
measurements on Vessel i (including t=0): A t i => A t i - A0i . Hence, all profiles start at the
zero point: t=0 is equivalent to %Diss=0. With the Hanson Dissolution System Drivers
installed, the AutoPlus will pump, but the E-Probes (or Auto-Probes) will not lower.

 Zero Vessels without pump: Same as Zero Vessels, but without a pump cycle. This
action can be used when using basket methods where the baskets are moved into the
vessels at time zero.

 Measure Infinity Value: Does not operate with the Hanson Dissolution System Drivers
installed.

 Bath Parameter: Changes the bath parameters (e.g., to turn off the heater and stirrers at
the end of the Dissolution Run).

 Lower/Lift Probes: Moves the probes if automated probes are installed. It is not
necessary to use the Lower/Lift Probes actions when the Hanson Dissolution System
Drivers are installed to actuate E-Probes or Auto-Probes for the Measure Vessels action.

 Sampler Position: Does not operate with the Hanson Dissolution System Drivers
installed.

 Change Sampler Tray: Does not operate with the Hanson Dissolution System Drivers
installed.

Appendix A Dissolution Run Parameters – 69 –

Hanson Dissolution System Drivers Operation Manual 81-200-807-D

Note: Clicking the Check button will perform a consistency check of all actions
that have been setup in the PreRun Sequence, Dissolution Run, and PostRun
Sequence. If any inconsistencies are detected, the Consistency Check Results
window pops up, and shows all Errors (E) and Warnings (W).

PostRun Seq.
The PostRun Seq. tab of the Dissolution Run Method Parameters dialog box contains a table
that allows you to set up a sequence of actions that are performed after the conclusion of the
dissolution test. The table contains three columns:

# Column
The # column shows the line number of the sequence of actions. The actions are performed
sequentially from the lowest line number to the highest.

Action
The Action column defines the actions in the sequence.

Parameters
The Parameters field shows the values of the parameters set in the appropriate Setup dialog
box. If no parameters have been specified, the Parameters field displays the message
>>> not set <<<. The requirements for the Parameters field are determined by the action
specified.

The possible actions are defined in a drop-down list, which is displayed by activating the
Action field and choosing the down arrow. These actions are:

 Remote Operation: Does not operate with the Hanson Dissolution System Drivers
installed.

 Print Report: Prints the Dissolution Report at the conclusion of the test. Selecting Setup
allows the user to select the report style and the destination (printer, screen, or file).

 Operator Message: Pops up a window with a message to the operator.

 Wash Cycle: Does not operate with the Hanson Dissolution System Drivers installed.

 Flow Rate Test: Does not operate with the Hanson Dissolution System Drivers installed.

Appendix A Dissolution Run Parameters – 70 –

Hanson Dissolution System Drivers Operation Manual 81-200-807-D

 Measure Blank: Measures a blank spectrum. A good blank measurement shows a flat
baseline with A<10-3 AU. Operating with the Hanson Dissolution System Drivers installed,
this action requires that the cell be pre-filled by using a UserMacro action (such as
hrTransfer) to fill the Cell Position 1 with blank media from an appropriate source before
the measurement. This action should be prioritized to run before the Measure Blank
action. Operating with the Hanson Dissolution System Drivers installed, the Channel is
always set to 1. The cell position measured is always Position 1.

 Measure Control: A sample of known concentration used to validate the calibration.

Operating with the Hanson Dissolution System Drivers installed, this action requires that
the cell be pre-filled by using a UserMacro action (such as hrTransfer) to fill the Cell
Position 1 with the control solution from an appropriate source before the measurement.
This action should be prioritized to run before the Measure Control action. Operating with
the Hanson Dissolution System Drivers installed, the Channel is always set to 1. The cell
position measured is always Position 1.

 ReMeasure Standard: Prompts the operator to remeasure the standard used to calculate
the dissolution results. The ChemStation options are: 1) Replace existing Standard: The
“old“ standards will be replaced by the remeasured standards; 2) Average with existing
Standards: The remeasured standards are appended to the existing standard; hence,
they will be averaged.

Operating with the Hanson Dissolution System Drivers installed, this action requires that
the cell be pre-filled by using a UserMacro action such as hrTransfer to fill the Cell
Position 1 with the standard solution from an appropriate source before the
measurement. This action should be prioritized to run before the ReMeasure Standard
action. Operating with the Hanson Dissolution System Drivers installed, the Channel is
always set to 1. The cell position measured is always Position 1.

 Save Dissolution Result: Saves the Result file (*.dr) on the hard disk. This should
always be the first action in the PostRun Sequence!

Caution: If a Dissolution Run is stopped before the action Save Dissolution

Result, the Result file must be saved manually (FileSave Dissolution Result
As…), even though the Result file name is entered at the beginning of the
dissolution run, and is also shown on the graphical user interface!

 Switch Off Lamps: Switches off the UV (deuterium) lamp and/or Visible (tungsten) lamp.

Appendix A Dissolution Run Parameters – 71 –

Hanson Dissolution System Drivers Operation Manual 81-200-807-D

 Switch Off Bath: Switches off the heater and/or stirrer.

 Goto Position: Does not operate with the Hanson Dissolution System Drivers installed.

 Pump: Does not operate with the Hanson Dissolution System Drivers installed.

 Lower/Lift Probes: Moves the probes if automated probes are installed. Lower Probes
and Lift Probes actions must be separated by another action for the actions to be
effective.

 User Macro: Executes a user-defined macro. The AutoPlus Driver contains the following
macros which can be called from the User Macro function in the PreRun Sequence:

o hrTransfer (Volume, Source, Dest): Transfers a volume from a source to a

destination. Sources can be A, B, C, D, or X for Current Bath (e.g., to transfer 5.0 mL
from the Current Bath to D, enter hrTransfer 5.0, X, D in the Parameters field).

o hrPrime: Runs the prime function with the parameters selected in the AutoPlus icon.

o hrWash: Runs the wash function with the parameters selected in the AutoPlus icon.

o hrRun (Protocol Number): Runs an AutoPlus protocol (e.g., to run AutoPlus Protocol
3, enter hrRun 3 in the Parameters field).

 Wait: User-defined wait time. Can be used for time-limited actions indicated by an
Operator Message.

Note: Clicking the Check button will perform a Consistency Check of all actions
that have been setup in the PreRun Sequence, Dissolution Run and PostRun
Sequence. If any inconsistencies are detected the Consistency Check Results
window pops up, and shows all Errors (E) and Warnings (W).

Appendix A Dissolution Run Parameters – 72 –

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