Help Me Write A Narrative Essay

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Narrative Essay Help Me Write A Narrative EssayHelp Me Write A Narrative Essay
 Dna Sequences Using Polymerase Chain Reaction
Amplification of 16S Ribosomal DNA Sequences using Polymerase Chain Reaction
Edwina Abou Haidar,
Houssam Al Koussa,
Mary AbedAlAhad.
Department of Biology, Lebanese American University, Byblos, Lebanon

Abstract The 16s rRNA gene sequencing is a widely common amplicon sequencing method used to
identify and compare bacteria in a given sample. This method is well established and allows to study
phylogeny and taxonomy of complex microbiomes. In this study, an unknown sample of extracted
microbial DNA was analyzed by performing the polymerase chain reaction followed by agarose gel
electrophoresis. The results were accurate since three distinct bands (1500 basepairs) corresponded
to our sample (duplicates) and the positive control. This indicates clearly the amplification of the
16s rRNA gene whose further sequencing technique constitutes a pivotal tool in the accurate
identification of bacterial isolates as well as the discovery of novel bacteria in clinical microbiology
laboratories.
Abbreviations
rDNA: ribosomal DNA rRNA: ribosomal RNA bp: base pair
PCR: Polymerase Chain Reaction Introduction Physiological and biochemical tests constituting the
basis of conventional differentiation between bacterial species are somehow cumbersome, consume
a lot of time and require different approaches [1]. Furthermore, the commercial identification systems
failed to identify commonly encountered bacteria and uncommon isolates. In fact, these commercial
systems
 Alternative Options For Public Transport Essay
5. Alternative options for public transport:
To ensure public transport in the command area available alternatives are:
1) Establishment of Metro Rail Service:
Under the fast track project of Bangladesh Government, elevated Metro rail system is
implementing in the capital to link between north and south of the city. Dhaka Mass Transit
Company Ltd. (DMTCL) estimate £2.20 billon (£109 million per km) needed to construct the
20.10 km long Uttara 3rd Phase (North Dhaka) to Bangladesh Bank (South Dhaka) (Dhaka Mass
Rapid Transit Development Project, 2016). The option for Metro Rail system in the proposed
alignment will be the gigantic in terms of investment, about £2.45 billion shall be required to
implement such gigantic project. Moreover, selected area is out of the urban zone which will not
give the actual objects of enhance public transport.
2) Construction of Elevated Expressway from Dhaka(Mirpur Bridge) to Nabinagar (Dhaka):
Bangladesh Bridge Authority, Bridges Division, Ministry of Bridge Transport and Bridges,
implementing19.73 kilometer long Dhaka Elevated Expressway PPP Project having total cost of
£894 million (BDT 8940 crore) indicates that to construct 22.50 kilometer long expressway
government need mammoth investment of about £1.02 billion (£45.31 million per km) (Bangladesh
Bridge Authority, 2016). Besides, Huge investment will not facilitate only the public transport, but
private vehicle will also get the same benefit from the elevated infrastructure
 My Field Experience At S Bryan Jennings Elementary
My field experience took place at S Bryan Jennings Elementary in Mrs.Reid s second grade
classroom for a little over a month. During my time within the classroom I observed the subjects:
reading, mathematics, and phonics, science, and writing. I also observed a teacher planning period.
When I was within the classroom I observed the techniques the teacher used in the subjects, the
programs used, the children s abilities with the subjectand/or topic, the students struggles within the
subject and/or topic, the teachers struggles with students who did not understand, and the stereotypes
that applied and/or did not apply to the classroom, teacher, and students. Within this dissertation, I
will describe and focus on these observations in a more specific way following with observed
examples.

Mrs. Reid has 16 students consisting of five females and eleven males. One of the five females
was a minority; she was Asian. Five of the eleven males were also minority decent, such as
oriental, African American, ect. On a side note, within the second grade class there was also
Hattian decent students. Five of the eleven males left at some point during the week for more
specific teachings to their ability, such ESE, ELOS, or gifted; the same five minority males were
NOT the same five that left during the week. Only one of the five males that left during the week
was for gifted; he was Caucasian. The other four that left were of the minority. None of the four
were Asian.

My first subject
 Wage Workers In The 1800s
In society, many make a living by working for others to get the necessities they need. Workers face
many struggles such as wage cuts, horrendous work conditions, an increase in hours, due to these
conditions they are labeled wage slaves. These individuals have no choice but to work in these
conditions. The growth of industry in the United States made corruption easier and made
employers richer while the poor stayed poor. The employers justify their actions by arguing that
the employees have a choice to go and work for someone else. However, workers do not have the
choice to work for others since most employers were using the same method to make a profit.
Workers are treated unfairly, but they use many effective techniques such as strikes... Show more
content on Helpwriting.net ...
Workers became more numerous, better organized, more disciplined, and more successful even
though employers tried to stop them (Who Built America 113). Many of these workers led
strikes for better hours, better wages and better conditions. When comparing the 1800s to today,
we see that strikes were very effective, an example of this is shown in the 8 hour movement.
Unions helped win more than 60 percent of the strikes waged in 1889 and 1890 (Who Built
America 113). Due to these workers, employees now face fewer obstacles. Whenever workers felt
as though they were being taken advantage of they could refuse to work, if they withdraw their
cooperation, every social institution can be brought to a halt (Brecher 5). Without workers, the
employers will have no one to complete products therefore not being able to make a profit.
Subsequently, employees will have leverage over the employers so they can finally sit down and
negotiate their conditions. This was shown in the Michigan Central Railroad where workers
fought for their wages to increase by two dollars. The workers exhibited their resistance when
streetcars, wagons, and buggies were stopped; tanneries, stoneworks, clothing factories,
lumberyards, brickyards, furniture factories, and a large distillery was closed in response to roving
crowds (Brecher 31). The result of this strike was victorious, and 2 dollars a day became the
standard wage in Galveston (Brecher 31). This strike was important because it exhibited to others
that if they unite then they can achieve better conditions. If the workers did not unionize they would
not have achieved equality in the workforce and better wages and conditions. Without resistance,
these workers would have never gotten negotiations or the necessities they need. Even if workers lost
because of outside interference they eventually had the power to change the minds of
 Regina Mills And Communication Styles Essay
Regina Mills and Communication Styles
The character Regina Mills from the show Once Upon a Time is an evil queen whom has set out
to have her vengeance to all the inhabitants of Fairy Tale Land also known as StoryBrooke. Regina
states for StoryBrooke, Maine to be Somewhere horrible, absolutely horrible, a place where the
only happy ending will be mine . (ABC Network) Regina begins trying to change her ways and put
her magic powers aside to have a chance for her own happy ending with her adopted son Henry.
However, her mother, Cora has come into town with a different plan in mind in which turns and Cora
is murdered, and Henry has been kidnapped. One of the methods Regina communicates is by using
her magic. Regina s communication styles vary from Dominant communication style, Contentious
communication style, and Dramatic communication style to non verbal communication. Regina does
not communicate very well due to three overwhelming reasons.
First putting evil behind her with the use of magic is difficult since it is all she has used since
she was young. As a young woman Regina s betrothed is murdered by her mother. This begins the
whirlwind in which life changes Regina into the Evil Queen. When Regina is not using magic, she
uses a dominant communication style to meet her own needs. For example, even when tied to a
post in the town s courtyard she states with tears in her eyes and power in her voice above all else
with every ounce of my being I regret that I was not
 False Imprisonment Vs False Imprisonment
A civil conspiracy, also known as collusion, is an arrangement between two or more parties to
deprive a third party of legal rights or deceive a third party to obtain an illegal objective. It is not
necessary that all the conspirators be involved in each stage of planning or know about all the
details. Forgery is the process of making, adapting, statistics, or imitating objects, or documents with
the intent to deceive a seller or the publics opinion. In the case of a bad check, the laws and
punishments very widely state by state. Embezzlement is defined in most states as theft and/or
larceny of assets by a person in a position of trust or responsibility over those assets. Embezzlement
usually occurs in the employment and corporate settings. #2 All states have false imprisonment laws
to protect against false imprisonment. To prove a false imprisonment claim in a civil lawsuit, the
following details must be present: 1.There must have been a willful detention 2.The detention must
have been without consent 3.The detention was unlawful... Show more content on Helpwriting.net ...
While physical force is often used, it is not required to constitute false imprisonment. There is a
delicate balance between one person s right to freedom of speech and another s right to protect
their good name. It is often difficult to know which personal remarks are proper and which are not.
Defamation law changes as you cross state borders, but there are normally some accepted standards
that make laws similar no matter where you are. If you think that you have been the victim of a
defamatory statement, whether slander or libel, then you will need to file a lawsuit to recover.
Usually speaking, to win your lawsuit, you must show
 Similarities And Differences Of African And Native...
African and Native American religions and there similarities
There are may religions in the world, but through out the different religions there are many
crossovers and similarities. Mattie Stepanek said It doesn t matter how you pray. Just pray. All
religions are beautiful and they all have one common belief. There s something bigger and greater
than us that can give us and take from us life. It is better than the here and now . Religion itself
means a belief in and worship of a greater power (dictionary.com). By people believing in any kind
of religion, it means that similarities can start there. With so many religions having similarities, it
might be hard to distinguish from certain religions from another. Religions like of African and
Native Americans might not at first seem alike, but as you look deeper into their religions and see
they have similarities. These similarities are attributed as beliefs, rituals, and history. Their growths
and struggles might not have happened at the same time, but both religions are still celebrated today.
Like in any religion the people have certain beliefs. In the african religions there is no single
religion. Researchers try to identify similarities in world views and ritual processes across the
african boundaries. African Religions are polytheistic; which means they believe in more than one
god. They believe in one overall god, but also ancestral spirits. Most African religion s creation story
reveal the creation of humans more than
 The Safety Of A Car Vehicle
Most of the automotive companies believe that the next generation of cars are going to be
autonomous vehicles, but not all of them have the same opinion about it. Some companies want
to bring a fully autonomous car to reality, which wouldn t need any human interaction to drive the
car, while other ones are interested in driverless cars, which need humans to drive them. Both
autonomous and driverless concepts give cars more control over themselves, and both aim to
give more comfort for mankind, thus providing an easier life for drivers and passengers, but the
idea of an autonomous car on the roads raises some questions about the safety of it and the
responsibility over a car like this. A driverless car can have most of the autonomous features, it
just needs someone to drive it, and then the control would also be from a person that would be
more watchful and take responsibilities over the car. Ford is going to launch an autonomous car.
The company already has a semi autonomous one in market, the Fusion hybrid, and they say that
the car is automated, but by automated they mean that it is capable of automated operation under
the supervision of a human driver. Ford already have one step in automated driving, and in five
years they are going to bring two new innovative features that are going to be essential for self
driving cars; one is the fully automated parking that allows the vehicle to park itself with no help
from the driver, the driver just have to press a button, then
 The Logo Of Bacardi
The logo for the Bacardi brand has been changed quite a few times. It has been recently rebranded
and given a more vintage look inspired by the Bacardi hand drawn bat designs from the early
1900s. The bat logo was chosen because from the beginning many of Bacardi s potential clients
were illiterate, and the image of the bat helped them to remember the rum. It is also used because
it stays true to the integrity of the original symbol. In Cuban and Spanish heritage bats represent
good health, fortune, and family unity. This new/old look makes Bacardi look a little bit more
sophisticated and also quirky. Far from the cartoon style that it once had, in the new logo, they got
rid of the yellow/gold colour which makes it look cleaner, it s a smaller and... Show more content on
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The Bacardi logotype is much more eye catching when looked upon and the letter design leaves
little space for flair. The accent on the I reminds the consumer that it is a product from a Cuban
and Spanish culture, it also looks more bold than the previous version and along with the particular
angular quality with the two A s, this sets a reminder of their Untamable feature. Bacardi has
changed to this more straight style of logo that resembles that of vodka logos which almost puts
them directly opposite to the classical rum style. Usually, rum brands want their logos to create an
exotic feel with persistent modernity. This Bacardi logo is what is going to make their rum stand
out, it will be unique compared to the rest of the rum brands. Usually rum is associated with palm
trees and pirates, this simple yet memorable logo will appeal to the crowd of consumers nowadays
that accepts respectability and slick new professionalism. This way Bacardi demonstrates the
understanding that they have developed in the way that they are targeting their audience and the
way that they want to advertise their
 Ethical Egoism vs Deontological Egoism
3) Can Deontological Egoism avoid all the problems that confront unconstrained ethical egoism? In
this essay I will argue that Deontological Egoism can rescue Unconstrained Ethical Egoism from a
large number of its problems and that DE offers a more plausible and attractive Egoism theory
Understanding Moraltheories The goal of a moral theory is to meet certain moral principles.
Various principles are argued for to be included as moral principles within a workable moral theory.
We therefore can analyze the specific moral theories of Ethical Egoismand Deontological Egoism
which fall under the umbrella of the Egoism class of theories and see if they take into account
certain moral principles that are important in order for a theory to be... Show more content on
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Valuing the interests of one group or oneself over another group or others without reason to justify
these differences in treatment is arbitrary. Why should the interests of others matter to me? Moral
views exist in the world that place people into groups, racism, anti

semitism, sexism, and nationalism. According to these views one group is deemed better or
more worthy than another. People might feel that belonging to one ethnic group, race or country
makes them more superior than another group. Racists for example like to argue that they are
more superior to blacks , and that blacks are stupid, lazy and crazy. In order to feel superior or to
warrant taking more rights and benefits for oneself a racist will argue that differences indeed exist
between him and the blacks and that these differences advocate different treatment. We know that
these implied differences are not actually true. If all relevant things are equal there is no reason to
treat one better or worse than another. Ethical egoists are guilty of discriminating on arbitrary
grounds (race, ethnicity, gender) Ethical egoism is similar in that we are told to value ourselves
more than others and we then divide the world up into two groups, oneself and the rest of the world.
My interests (even wants for trivial interests like luxuries) are
 Holy War Research Paper
has there ever been a just war? The theory has been criticised for being hopelessly unrealistic ,
idealistic, assuming a very high standard of morals on both sides and failing to take into account the
multiple reasons relating to international relations and internal affairs that contribute to war. For
example, intent can be highly complex and Jones argues that to select a just cause is a moral fiction:
wars have multiple causes and to choose one as justification is unlikely to do justice to this
complexity (Rodger, 2013).

With that being said it is likely (hypothetically) if Jesus killed someone, it would spark up this
Holy War if you will, in which the Pharisees did not really need much of a reason because they
wanted to kill ... Show more content on Helpwriting.net ...
Because in each man s own eyes they are completely justified in what they are doing. Which is
what the Pharisee s thought when they decided to crucify Jesus on the cross. Because to them,
their actions were Just . They were doing what they thought was best for the country because Jesus
to them was converting and twisting their rules and beliefs that did not sit right with them. So that
is why they decided to have a Just cause and decided to plot to kill Jesus, because he was messing up
their way of life. So that is why I believe that the just war theory falls short. Because:

has there ever been a just war? The theory has been criticised for being hopelessly unrealistic ,
idealistic, assuming a very high standard of morals on both sides and failing to take into account the
multiple reasons relating to international relations and internal affairs that contribute to war. For
example, intent can be highly complex and Jones argues that to select a just cause is a moral fiction:
wars have multiple causes and to choose one as justification is unlikely to do justice to this
complexity (Rodger,
 Red Meat Research Paper
crowleys.butchery.com.au The Nutritional Values of Red Meat

Red meat is full of all sorts of nutrients that are beneficial for the growth and health of the human
body. The definition of this meat at least in Australian terms is the meat that comes from cattle, goat
and sheep. It does not include meat from such animals as pigs, kangaroo, chickens, turkey and
buffalo. When you purchase red meat, you will notice that it contains lean or muscle tissue along
with fat tissue, which can be both throughout the lean tissue as marbling or around the outside of the
particular cut of meat. A butcher typically trims off this excess fat to ensure the cut is as lean as
possible.

Protein Content

The protein content of red meat ranges from 20g up to 36g per 100g, depending on whether it is raw
or cooked. During the cooking processes, nutrients in this meat become more concentrated, thus, the
increase of protein per 100g of cooked red meat. In addition, this type of protein is more easily
digestible than certain vegetarian sources of protein are in the human body. Along with being such a
rich source of protein, this meat s protein also provides all the essential amino acids, namely lysine,
methionine, threonine, tryptophan, phenylalanine, isoleucine, leucine and valine. ... Show more
content on Helpwriting.net ...
The liver of the animals in this class of meat provides high levels of folate and vitamin A unlike the
lean tissue.
 Anxiety And Panic Disorders
Anxiety can have such distress that it hinders with the power to lead a normal life. If anxiety
causes worry and fear are constant and overwhelming, and can be disabling. A Panic disorder is a
condition that one s has a feeling of terror that comes suddenly and again and again with no warning.
Other symptoms of a panic attackinclude sweating, chest pain, shivers and it is an unusually strong
or irregular pulse. It can seem like you are having a heart attack or going crazy. Although, a panic
disordercan be frightening but normally it does not hurt others.

However, panic disorder should be treated by a physician it the panic attack continues. There are
some minor treatments that help an individual that is dealing with a panic disorder.
 System Description And Justification Of A Custom Built...
System Description and Justification Our customer has requested a custom built home PC to replace
an older, outdated system. The customer has identified specific requirements that the PC must meet.
These requirements are listed in Table 1. Component selection to meet the requirements listed in
Table 1 is detailed below. All components are new, not refurbished. The individual components with
price breakout are listed in Table 2. Diagram 1 is a graphical representation of the system
motherboard connections. Diagram 2 is a graphical representation of the motherboard s Input
/ Output (I/O) connections. Diagram 3 is a graphical representation of the power supply connections.
Diagram 4 is a graphical representation of the system case... Show more content on Helpwriting.net
...
Avoid small form factor PC cases. HTPC and Mini Tower cases are the smallest available, starting
at approximately 11 inches in height, and most susceptible to damage. Super Tower cases are
very large at two feet or taller. They are also the most expensive option, costing $200.00 and up.
Full Towers are 20 inches or taller. However, the least expensive Full Tower is still four times as
expensive as the starting Mid Towers. Mid Towers are 16 inches or taller in height, and are the
best value for a custom built system. The case needed to be compatible with a full size ATX
motherboard, since ATX had the widest compatibility with the different components that were
going to be required for this build. The case also had to have two (2) external USB 2.0 ports, a
minimum of; one (1) external 5.25 drive bay to install an optical drive, one (1) external 3.5 drive
bay to install an internal card reader, and three (3) internal hard disk drive bays. The Gigabyte GZ
ZA2 Black ATX Mid Tower Computer Case was one of the most cost effective choices that met or
exceeded all of the requirements. Item 2. Minimize the effects of Carpal Tunnel Syndrome.
According to Cornell University, there is not definitive proof that ergonomic devices actually
provide a health benefit or prevent Carpal Tunnel Syndrome (Hedge, 2015). User education and
proper positioning will provide for the best ergonomic arrangement of the user s computer
workstation and mouse (Hedge, 2011).