B.SC Biotech
B.SC Biotech
BIOTECHNOLOGY
Bachelor of science (B.Sc.) Semester scheme
Curriculum structure for upgraded programme – 2024-25
In accordance with SEP
Sl. Course/ Title of Paper Subject Teaching Semester Internal Total Credits Examination
No Paper Code Category hours end assessment marks duration
exams
Semester - 1
1 BT – MC Biomolecules MC – T 03 80 20 100 03 3Hrs
-I and
Practical - MC – P 04 40 10 50 02 3 Hrs
II
9 Project/ 02 40 10 50 02 --
Dissertati
on/
internship
Total 09 160 40 200 07 --
Unit I 10 Hrs
Basic Cytology: The cell theory, ultrastructure of plant and animal cell, different types of Cells-
Prokaryotes and eukaryotes. Cell cycle and its regulation; Cell-ECM and cell-cell interactions; cell
motility and migration; cell death: different modes of cell death and their regulation (apoptosis,
necrosis, necroptosis, autophagy, senescence etc.).
Unit II 14 Hrs
Genetics: Monohybrid & dihybrid crosses, back-crosses, test-crosses, analyses of autosomal and sex
linkages, genetic mosaics, genetic epistasis in context of developmental mechanism. Chromosomal
aberrations: Numerical and structural.
DNA Replication and DNA Repair: DNA polymerases, mechanisms of DNA replication in
prokaryotes and eukaryotes DNA replication models, Mutagens and DNA damage, DNA repair and
recombination.
Unit IV 12 hrs
Translation: protein translation machinery, ribosomes - composition and assembly; universal
genetic codes, degeneracy of codons, Wobble hypothesis; Iso-accepting tRNA; mechanism of
initiation, elongation and termination; post-translational modifications. Protein sorting and targeting.
Suggested Readings:
1. Alberts, B., Johnson, A., Lewis, J., Raff, M., Roberts, K., & Walter, P. (2008).Molecular Biology
of the Cell (5th Ed.). New York: Garland Science.
2. Lodish, H. F. (2016). Molecular Cell Biology (8th Ed.). New York: W.H. Freeman.
3. Krebs, J. E., Lewin, B., Kilpatrick, S. T., & Goldstein, E. S. (2014). Lewin's Genes XI.
Burlington, MA: Jones & Bartlett Learning.
4. Cooper, G. M., & Hausman, R. E. (2013). The Cell: a Molecular Approach (6th Ed.).Washington:
ASM; Sunderland.
5. Hardin, J., Bertoni, G., Kleinsmith, L. J., & Becker, W. M. (2012). Becker's World ofthe Cell.
Boston (8th Ed.). Benjamin Cummings.
6. Watson, J. D. (2008). Molecular Biology of the Gene (5th ed.). Menlo Park,
CA:Benjamin/Cummings.
PART-A
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PART-C
Unit I 12 hr
Carbohydrates: Definition, Classification, Fischer and Howarth structure of monosaccharides and
Disaccharides - Ribose, Glucose, Galactose and Fructose, Sucrose, Maltose, Lactose. Reducing and
non-reducing sugars.Polysaccharidesclassification: homo and heteropolysaccharides, Structure of
Starch and Chitin. Carbohydrates in health and diseases.
Lipids: Definition, Classification and biological properties. Fatty acids- unsaturated and saturated
fatty acids. Essential fatty acids and their biological importance.Biological role of lipids-
Phospholipids, Glycolipids and Cholesterol.
Unit II 14 hr
Proteins and Enzymes: Amino acids- general structure, essential and non-essential amino acids,
classification based on polarity, Titration of amino acids - zwitter ionic structure. D-and L- amino
acids. Structure and properties of peptide bond. Structural organization of proteins -primary,
secondary, tertiary and quaternary structure of proteins, Biological importance of proteins.
Enzymes: Nomenclature and classification, mechanism of enzyme action – Lock and key
hypothesis, Induced Fit hypothesis. Enzyme kinetics – Michelis – Menton equation and Lineweaver-
Burk plot. Enzyme inhibition. Application of enzymes in Agriculture,Industries and Medicine.
UNIT-III 08 hr
Nucleic Acids: Nucleosides & Nucleotides - structure & nomenclature. DNA-Types, Secondary
structure of DNA (Watson and Crick model), RNA- Types and biological functions, structure of t-
RNA (Clover leaf model).
UNIT-IV 14 hr
Basics of metabolism:Basic thermodynamics: free energy, enthalpy and entropy.
Basic terminologies: Anabolism, catabolism, primary metabolism and secondary metabolism.
Carbohydrate metabolism: Glycolysis and its regulation, gluconeogenesis. TCA cycle and electron
transport chain. Calvin Cycle, C4 and CAM pathway.
Lipid metabolism: Beta-oxidation, Biosynthesis of fatty acids.
Amino acid Metabolism: Glucogenic and ketogenic amino acids, general pathways of
degradation,Urea cycle, General pathways of synthesis of amino acids.
Metabolism of nucleotides: Biosynthesis of purines and pyrimidines and degradation of purines and
pyrimidines.
Suggested Readings:
1. An Introduction to Practical Biochemistry, 3rd Edition, (2001), David Plummer; Tata
McGraw Hill Edu.Pvt.Ltd. New Delhi, India
2. Biochemical Methods,1st Edition, (1995), S. Sadashivam, A. Manickam; New Age
International Publishers, India
3. Introductory Practical biochemistry, S. K. Sawhney & Randhir Singh (eds) Narosa
Publishing. House,New Delhi, ISBN 81-7319-302-9
4. Experimental Biochemistry: A Student Companion, Beedu Sasidhar Rao& Vijay
Despande(ed). I. K International Pvt. LTD, New Delhi. ISBN 81-88237-41-8
5. Standard Methods of Biochemical Analysis, S. K. Thimmaiah (ed), Kalyani Publishers,
Ludhiana ISBN 81-7663-067
6. Principles of Biochemistry: 6Eds. Albert L Lehninger, Tata_McGrawHill Publications, New
York, USA.
PART-A
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PART-C