International Journal of Trend in Scientific Research and Development (IJTSRD)

Volume 9 Issue 1, Jan-Feb 2025 Available Online: www.ijtsrd.com e-ISSN: 2456 – 6470

An Evaluation of Wound Healing Activity of Meshashrungi

Phala (Gymnema Sylvestre R.Br.) with Special Reference to
Excised Wound on Wistar Albino Rats
Dr. Bhuvaneshwari1, Dr. Gururaj S Kulkarni MD (Ayu) Ph.D.2,
Dr. Shashidhar S Sarawad MD (Ayu) Ph.D.3
1
PG Scholar, Department of PG Studies in Dravya Guna,
2
Professor, Department of PG Studies in Dravya Guna,
3
HOD and Professor, Department of PG Studies in Dravya Guna,
1,2,3
BVVS Ayurveda Medical College and Hospital, Bagalkot, Karnataka, India

ABSTRACT How to cite this paper: Dr.

Wounds are physical injuries that results in an opening or break of Bhuvaneshwari | Dr. Gururaj S Kulkarni
the skin that cause disturbance in the normal skin anatomy and | Dr. Shashidhar S Sarawad "An
function. Wound healing is an interaction of complex cascade of Evaluation of Wound Healing Activity
of Meshashrungi Phala (Gymnema
cellular and biochemical actions leading to the restoration of
Sylvestre R.Br.) with Special Reference
structural and functional integrity along with regaining of strength of to Excised Wound on Wistar Albino
the injured tissue. Rats" Published in
It also involves continuous cell to cell interaction and cell matrix International
interactions that allow the process to proceed in different overlapping Journal of Trend in
phases including inflammation, wound contraction, re- Scientific Research
and Development
epithelialisation, remodelling etc. Many Ayurvedic plants have
(ijtsrd), ISSN:
important role in the process of wound healing. Among them, 2456-6470, IJTSRD73880
Meshashrungi phala is indicated in Vrana. The present research work Volume-9 | Issue-1,
was conducted to analyse the wound healing action of the fruits of February 2025, pp.418-421, URL:
Meshashrungi. www.ijtsrd.com/papers/ijtsrd73880.pdf
KEYWORDS: Meshashrungi (Gymnema sylvestre R.Br.); Wound Copyright © 2025 by author (s) and
healing activity International Journal of Trend in
Scientific Research and Development
Journal. This is an
Open Access article
distributed under the
terms of the Creative Commons
Attribution License (CC BY 4.0)
(http://creativecommons.org/licenses/by/4.0)

INTRODUCTION
Wound is a disease according to system of Ayurveda in different overlapping phases including
while it may be a sign in the modern system. In inflammation, wound contraction, re-epithelialisation,
Ayurveda it is compared to vrana with various remodelling etc2.
classifications like sadhyovrana, dustavrana which The wound heals naturally but the role of a wound
can be correlated to fresh cut wounds like excision, healing agent is to prevent infection and fasten the
incision etc. Wounds are physical injuries that results
healing process. There is a need to find out plant
in an opening or break of the skin that cause sources which shows the properties like rapid
disturbance in the normal skin anatomy and function1. granulation changes, easy availability and economical
Wound healing is an interaction of complex cascade feasibility.
of cellular and biochemical actions leading to the Research on wound healing drugs is a developing
restoration of structural and functional integrity along
area in modern biomedical sciences. Scientists who
with regaining of strength of the injured tissue. It also are trying to develop newer drugs from natural
involves continuous cell to cell interaction and cell
resources are looking towards Ayurveda. Several
matrix interactions that allow the process to proceed
drugs of plant, mineral and animal origin are

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 International Journal of Trend in Scientific Research and Development @ www.ijtsrd.com eISSN: 2456-6470
described in Ayurveda for their wound healing experiment was treated in the similar manner as
properties under the term vranaropaka and have mentioned above4.
important role in the process of wound healing.
Test Drug
Among them, Meshashrungi phala is indicated in
Aqueous extract of Meshashrungi fruits (Gymnema
vrana. It has tikta rasa and it is also indicated in sylvestre R.Br.)
kushta, meha, kasa, krimi and visha in guduchyadi
varga of Bhavaprakasha nighantu3. The present study Standard Drug
was intended to see the efficacy of aqueous extract of The standard drug used for wound healing activity
Meshashrungi fruits in wound healing activity. evaluation is Povidine Iodine.
AIMS AND OBJECTIVES Animals
Evaluation of wound healing activity of aqueous The study was carried out in Wistar albino rats of
extract of fruits of Meshashrungi (Gymnema sylvestre body weight ranging from 200-250 gm. They were
R.Br.) obtained from well-established animal house H.S.K
College of Pharmacy, Bagalkot, Karnataka. They
MATERIALS AND METHODS
were maintained on feed of food and tap water was
Fresh fruits of Meshashrungi were collected from the given. The temperature and humidity were kept at
natural habitat, Bagalkot.
optimum, and animals were exposed to natural day
Excision wound model was selected for the study to night cycles.
determine rate wound contraction and
Experimental procedures were undertaken according
epithelialization period. Animals were anaesthetized
to the principle guidelines of animal care with prior
by using 60 mg/kg single dosage of Ketamine
permission from Institutional Animal Ethical
hydrochloride before the animal experiment. The
Committee.
dorsal fur of the animals was shaved with electric
clipper and excision wound of size 200 mm2(4 cm2) Drug Preparation: Aqueous extract of fruits of
was created using toothed forceps, surgical blade and Meshashrungi (Gymnema sylvestre R.Br.)was
pointed scissors. The preparation was applied twice a prepared at H.S.K College of Pharmacy, Bagalkot.
day from the day of infliction of wound. Until Dose Fixation –Quantity sufficient as per topical
complete epithelialisation, the entire wound was left route of administration with the help of spatula.
open. All the 3 groups of animals taken for
Drug dosing schedule
Frequency: two times per day
Total no of days: 21
Healthy Wistar albino rats were divided into three groups of six animals in each group (n = 6) and were kept in
separate cages.
Showing grouping of animals
Route of
Sl. no. Group Drug applied Dose
Administration

Group 01 Control group No - -

Group 02 Standard group Povidine iodine QS Topical application
Aqueous extract of fruit of Meshashrungi
Group 03 Test group QS Topical application
(Gymnema sylvestre R.Br.)
Evaluation of wound healing activity
Wound healing activity was assessed in animal model by using transparency sheet and permanent marker on day
0, day 3, day 6, day 9, day12, day15, day 18, day 21 and wound closure was calculated of Test drug, Standard
drug & Control group animals.
Parameters employed for assessing the wound healing activity
1. Wound measurement
Wounds were inspected and photographed and progressive changes in wound area were monitored
planimetrically by tracing the wound margin on a transparency sheet every alternate day. The change in healing
of wound, i.e. the measurement of wound area on transparency sheet was expressed as unit (cm2). Wound
contraction was expressed as reduction of original wound size.

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2. Wound closing days and formation of dermis
Falling of scab, leaving no raw wound behind, was taken as the end point of complete epithelialization and the
days required for this were taken as period of epithelialization.
OBSERVATION AND RESULTS
The results obtained were analyzed by one way ANOVA followed by Dunnet’s multiple comparison ‘t’ test as
post hoc test, by using graphic instant software. A level of p<0.05 was considered as statistically significant.
Level of significance was noted and interpreted accordingly. The obtained data in each group are shown
accordingly in given tables.
Showing effect of aqueous extract of Meshashrungi phala (Gymnema sylvestre R.Br.) on wound healing
activity on Wistar albino rats
21 st
Groups O th day 3 rd day 6 th day 9 th day 12 thday 15 th day 18 th day
day
Group1 4.000±0. 4.000±0. 3.657±0 3.867±0. 3.583±0. 3.433±0. 2.940±0. 2.447±0
(Control group) 0 0 .12 08 11 10 12 .14
Group2 3.733±0. 2.565±0. 0.9967±0 0.8217±0 0.5050±0 0.2200±
4.000±0. 3.323±0
(Povidine 08 08 .14 .26 .11 0.07
0 .14
Iodine) * *** *** *** *** ***
Group 3
2.523±0. 1.633±0. 1.288±0. 0.8867±0 0.6000±
(Aqueous extract 4.000±0. 3.933±0. 3.578±0
13 15 12 .15 0.13
of fruit of 0 06 .01
*** *** *** *** ***
Meshashrungi)
All the values are expressed as Mean ± SEM, n =6, One way Analysis of Variance ( ANOVA) followed by
multiple comparison dunnet’s test. **p<0.01, ***p<0.001 as compared to the control group.

DISCUSSION in wound area were monitored plainmetrically by

Rasa of Meshashrungi fruit is tikta and it is known tracing the wound margin on a transparency sheet and
that tikta rasatmaka dravyas are rich in alkaloids and permanent marker on every alternative day that is on
saponin glycosides which helps in wound healing day 0, day 3, day 6, day 9, day12, day15, day 18, day
process. Animal experimental study was conducted to 21 and area of wound closure was calculated. The
evaluate wound healing activity in which the animals area of the wound started to reduce from 3rd day and
were divided into 3 different groups each group gradually it decreased on 6th ,9th,12th,15th,18th and
containing 6 Wistar albino rats. Animals were 21st day. Study showed that test drug aqueous extract
anaesthetized and a predetermined area of 200mm2 of fruits of Meshashrungi is effective and have more
was excised in the dorsal inter scapular region. All the significance in wound healing when compared to
groups received laboratory food and diet, standard control group.
group was applied with Povidine-iodine and the test
Probable mode of action:
group was applied with the aqueous extract of  Meshashrungi phala having tikta rasa which
Meshashrungi fruits for 21 days. Wounds were
helps in removal of debris and pathogens as it is
inspected and photographed and progressive changes

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having ruksha laghu guna, which plays important REFERENCES
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