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The Analysis of Biological Data
The Analysis of Biological
Data
Second Edition
Reproduction or translation of any part of this work beyond that permitted by Section 107
or 108 of the 1976 United States Copyright Act without permission of the copyright owner
is unlawful. Requests for permission or further information should be addressed to the W.
H. Freeman and Company Rights and Permissions Department. Grateful acknowledgment
for third-party permissions, which have been granted for material in this title not owned by
Macmillan Learning, appears in the Photo Credits section, which represents an extension of
this copyright page.
ISBN: 978-1-319156-71-8
10 9 8 7 6 5 4
W. H. Freeman and Company
One New York Plaza
Suite 4500
10004-1562
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www.macmillanhighered.com
To Sally and Wilson, Andrea and Maggie
Contents in brief
Preface
Acknowledgments
About the authors
Review Problems 2
14. Designing experiments
INTERLEAF 8 Data dredging
15. Comparing means of more than two groups
INTERLEAF 9 Experimental and statistical mistakes
Review Problems 3
Preface
Acknowledgments
About the authors
Review Problems 1
Review Problems 2
14. Designing experiments
14.1 Why do experiments?
Confounding variables
Experimental artifacts
14.2 Lessons from clinical trials
Example 14.2: Reducing HIV transmission
Design components
14.3 How to reduce bias
Simultaneous control group
Randomization
Blinding
14.4 How to reduce the influence of sampling error
Replication
Balance
Blocking
Example 14.4A: Holey waters
Extreme treatments
Example 14.4B: Plastic hormones
14.5 Experiments with more than one factor
Example 14.5: Lethal combination
14.6 What if you can’t do experiments?
Match and adjust
14.7 Choosing a sample size
Plan for precision
Plan for power
Plan for data loss
14.8 Summary
14.9 Quick Formula Summary
Practice problems
Assignment problems
INTERLEAF 8 Data dredging
15. Comparing means of more than two groups
15.1 The analysis of variance
Example 15.1: The knees who say night
Hypotheses
ANOVA in a nutshell
ANOVA tables
Partitioning the sum of squares
Calculating the mean squares
The variance ratio, F
Variation explained: R2
ANOVA with two groups
15.2 Assumptions and alternatives
The robustness of ANOVA
Data transformations
Nonparametric alternatives to ANOVA
15.3 Planned comparisons
Planned comparison between two means
15.4 Unplanned comparisons
Example 15.4: Wood wide web
Testing all pairs of means using the Tukey–Kramer method
Assumptions
15.5 Fixed and random effects
15.6 ANOVA with randomly chosen groups
Example 15.6: Walking stick limbs
ANOVA calculations
Variance components
Repeatability
Assumptions
15.7 Summary
15.8 Quick Formula Summary
Practice problems
Assignment problems
INTERLEAF 9 Experimental and statistical mistakes
Review Problems 3
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