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301 - Microbiology - 2020

The document is an examination paper for B.Sc. Sem.-V in Microbiology, focusing on Molecular Biology and Genetics of Prokaryotes. It contains instructions for students, a list of questions to answer, and specific topics related to DNA, transcription, translation, and mutations. Students are required to answer three questions from a selection of eight, with a compulsory question at the end.

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navchandiparidha
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2 views4 pages

301 - Microbiology - 2020

The document is an examination paper for B.Sc. Sem.-V in Microbiology, focusing on Molecular Biology and Genetics of Prokaryotes. It contains instructions for students, a list of questions to answer, and specific topics related to DNA, transcription, translation, and mutations. Students are required to answer three questions from a selection of eight, with a compulsory question at the end.

Uploaded by

navchandiparidha
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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Seat No.

: _______________

JG-123
January-2021
B.Sc., Sem.-V
CC-301 : Microbiology
(Molecular Biology & Genetics of Prokaryotes)
(New Syllabus)

Time : 2 Hours] [Max. Marks : 50

in
Instruction : (1) Students should write the answers from the question paper applicable
to them; either “New Course” or “Old Course” and it must be
mentioned at the beginning of the answer paper.

ir.
(2) Answer any three questions out of eight questions. Question No. 9 is
compulsory.
(3) Draw figures wherever necessary.
(4)
ls
Figures to the right indicate marks.
ea
1. Describe different experiments that convinced that DNA is a genetic material. 14

2. (A) Discuss contributions of various scientists in elucidation of DNA structure. 7


(B) Explain the following events of DNA replication with diagram : 7
.R

(1) formation of initiation complex


(2) leading and lagging strands
(3) proof reading
w

3. Differentiate between the initiation and elongation events of transcription and


translation. 14
w

4. (A) Describe salient characters of genetic code. 7


w

(B) Explain the role of cAMP and CAP in regulation of lac operon. 7

5. Describe giving one example the mode of action of physical, chemical and biological
mutagen. 14

6. (A) Replica plate technique conclusively proves the spontaneous nature of mutation.
Justify. 7
(B) Describe in detail the effects of mutation in protein coding gene. 7
JG-123 1 P.T.O. 1

www.Realsir.in Instagram : @GRealsir


7. Differentiate between : 14
(1) Horizontal and vertical gene transfer
(2) Generalized and specialized transduction
(3) F+ and Hfr cells
(4) Chromosome and plasmid.

8. (A) Discuss the process of transformation in Gram-negative bacteria. 7


(B) Enlist and describe different types of plasmids. 7

9. Give short and specific answers in 1-2 lines only : (any eight). 8
(1) Define nucleoid.

in
(2) What is phenotype ?
(3) Which were the two different elemental radioactive isotope we utilized by
Hershey and Chase in their experiment that verified genes were made of DNA ?

ir.
(4) Name the technique used by Rosalind Franklin, which provided crucial clues to
the Watson-Crick DNA model.
(5) Write the diagrammatic flow-sheet of central dogma.

ls
(6) Name the enzyme needed for unwinding of DNA during transcription.
(7) Which enzyme formylates the amino acid during the process of initiation of
translation ?
ea
(8) What are Shine-Delgarno sequences ?
(9) Define diauxic growth curve.
(10) What types of mutation results due to addition or deletion of nucleotides in an
.R

intron ?
(11) What are transposons ?
(12) Define auxotrophs.
w

(13) In which phase of bacterial growth cycle the competence is usually obtained ?
(14) Which type of plasmid imparts the ability to carry out conjugation ?
(15) Name the genes that are picked up by lambda phage from E.coli during
w

specialized transduction.
(16) What is the status of F’ ?
w

____________

JG-123 2

www.Realsir.in Instagram : @GRealsir


Seat No. : _______________

JG-123
January-2021
B.Sc., Sem.-V
CC-301 : Microbiology
(Molecular Biology & Genetics of Prokaryotes)
(Old Syllabus)

Time : 2 Hours] [Max. Marks : 50

in
Instruction : (1) Students should write the answers from the question paper applicable
to them; either “New Course” or “Old Course” and it must be
mentioned at the beginning of the answer paper.

ir.
(2) Answer any three questions out of eight questions. Question No. 9 is
compulsory.
(3) Draw figures wherever necessary.
(4)
ls
Figures to the right indicate marks.
ea
1. Describe different experiments that convinced that DNA is a genetic material. 14

2. (A) Discuss the structure of DNA. 7


(B) Explain the following events of DNA replication with diagram : 7
.R

(1) formation of initiation complex


(2) leading and lagging strands
w

(3) proof reading

3. Explain with diagram the process of translation in Escherichia Coli. 14


w

4. (A) Describe salient characters of genetic code. 7


w

(B) Explain the role of cAMP and CAP in regulation of lac operon. 7

5. Explain the following: Reverse mutation, nonsense mutation, frame-shift mutation,


transversion and conditional lethal mutation. 14

6. (A) Write short note on transposons. 7


(B) Describe in detail direct and indirect repair mechanisms. 7

JG-123 3 P.T.O. 3

www.Realsir.in Instagram : @GRealsir


7. Explain in detail the following events and differentiate between them : 14
F+ F–, Hfr  F– and F'  F–

8. (A) Discuss the process of transduction in bacteria. 7


(B) Enlist and describe different types of plasmids. 7

9. Give short and specific answers in 1-2 lines only : (any eight). 8
(1) Define allele.
(2) What is genotype ?
(3) Which were the two different elemental radioactive isotope we utilized by

in
Hershey and Chase in their experiment that verified genes were made of DNA ?
(4) Name the technique used by Rosalind Franklin, which provided crucial clues to
the Watson-Crick DNA model.

ir.
(5) Write the diagrammatic flow-sheet of central dogma.
(6) Name the enzyme needed for unwinding of DNA during transcription.
(7)

(8)
translation ?
What are Shine-Delgarno sequences ?
ls
Which enzyme formylates the amino acid during the process of initiation of
ea
(9) Define diauxic growth curve.
(10) What types of mutation results due to addition or deletion of nucleotides in an
intron ?
.R

(11) What are transposons ?


(12) Define auxotrophs.
w

(13) In which phase of bacterial growth cycle the competence is usually obtained ?
(14) Which type of plasmid imparts the ability to carry out conjugation ?
(15) Name the genes that are picked up by lambda phage from E.coli during
w

specialized transduction.
(16) What is the status of F ?
w

___________

JG-123 4

www.Realsir.in Instagram : @GRealsir

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