Name……………………………………………………………………………………………

I AM MUZAFALU
Uganda Advanced Certificate of Education

S.6 BIOLOGY PRACTICAL

BIOLOGY PAPER 3

3 hours 15 minutes

INSTRUCTIONS TO CANDIDATES:

- This paper consists of three questions.

- Answer all questions.

- Answers must be written in the spaces provided.
- Additional sheets of paper must not be inserted in this booklet.

FOR EXAMINER’S USE ONLY

Question Marks Examiner’s signature

Total

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 1. You are provided with specimen R which is freshly killed.
(a) With reasons Identify the; (06 marks)
i) phylum…………………………………………………………………………
reasons
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ii) class……………………………………………………………………………
reasons
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b) Examine the structural features of the FORE and HIND limbs.
(i) State the structural difference between the fore and hind limbs (04marks)
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(ii) Measure the full length of the hind limb and the full length of the fore limb from the
body to the longest toe of the specimen using a thread then a ruler.
(i) Record in (mm) the (03marks)
Full length of hind limb
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 Full length of fore limb
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Ratio of length of hind limb: length of fore limb

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State your observation.
From your observation, Explain the significance of your measurements to the
survival of the specimen in its habitat (04marks)
Observation
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Significance
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(b) Pin the specimen ventral side upper most, dissect the specimen to display the
alimentary canal trace for the:
(i) Blood vessels that supply blood to the organs responsible for absorption of digested
food to the blood circulation, storage of undigested food and left lung.
(ii) (ii) Blood vessels that carry blood away from the liver, organ responsible for filtration
of blood and left hind limb.

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(iii) Draw represent your dissection for part(i) and (ii)without the stomach and with heart
pinned anteriorly. (23marks)

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 2. You are provided with specimens. W, X, Y &Z and solution T. specimens X, Y & Z are
at different stages of growth.
(a) Peal one half of specimen W and cut the pealed half into small pieces. Crush them into fine
paste using a motor and the pestle. Transfer the paste into a clean beaker and add 20cm3 of
distilled water. Mix well and leave to stand for 1 minute. Filter to obtain an extract. Heat
the extract to a temperature 65oc, leave to cool and name it extracts W. Carryout tests
indicated in the table below on the extract, record your test, observations and conclusions.
(11marks)

Test Observation Conclusions

Biuret’s test

Benedict’s test

Iodine test

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(b) Obtain 10 seedlings from each of the sample’s X, Y and Z, remove radicle if present, crush
them separately to obtain fine pastes, add 14cm3 of distilled water, mix well then leave to stand
for 12 minutes. Filter to obtain only the clear part and transfer into separate test tubes, label them
accordingly.

Now obtain three test tubes and label them 1-3, to all add 1cm3 of W, to test tube 1 add 1cm3 of X,
to test tube 2 add 1cm3 of Y and to test tube 3 add 1cm3 of solution Z.

Table 2 shows a summary of the test tubes and their contents.

Table 2

Test Tube Contents

1 1cm3 W+1cm3 X
2 1cm3 W+1cm3 Y
3 1cm3 W+1cm3 Z

Incubate all the test tubes in a water bath maintained at 35oC-40oC for 55 minutes shaking
regularly, after record your observations and conclusions in the table below (03marks).

Table 3

Test Tube Contents Observations Deductions

1 1cm3 W + 1cm3 X

2 1cm3 W + 1cm3 Y

3 1cm3 W + 1cm3 D

(C) Obtain three test tubes 4-6 to all, add 2cm3 of solution T. follow the instructions in the table 4
below to add the remaining components in the test tubes.

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Table 4

Test Tube Contents

4 1cm3 T+1cm3 X
5 1cm3 T+1cm3 Y
6 1cm3 T+1cm3 Z

Record your observations and conclusions in table 5 below (06marks)

Table 5

Test Tube Contents Observations Deductions

3 1cm3 T + 1cm3 X

4 1cm3 T + 1cm3 Y

5 1cm3 T + 1cm3 D

(C) Explain your results in the following test tables:

(i) Table 3 (03marks)

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(i) Table 5 (03 marks)

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(b) What is the biological significance of the results in table 5 (02marks)

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(c) What was the purpose of the following operations in this experiment?

(i) Heating extract W to 65oC. (01mark)

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(ii) Leaving the extracts X, Y and Z to stand for 12 minutes. (01mark)

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3. Specimens A, B, C and D are of same plant organs. Using a hand lens; examine the specimens
in details and answer the following questions.
(a)i) Describe the arrangement of florets of specimen B (04marks).

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(ii) Describe the structure of a single floret from specimen B (04marks)

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(b) Suggest two advantages and one disadvantage of the arrangement of florets as described in (a)
(i) above to specimen B.
(i) Advantages (03marks)

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(ii) Disadvantages (02marks)

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(c) Position your left hand vertically and gently rub the anthers of specimen C on the back of your
hand.
(i) Record your observations (03marks)
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(ii) Suggest the significance of your observations in c(i) above to the plant from which specimen
C was obtained (02marks)
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(d) Give two differences and similarities in the structure of specimens A and D (02marks)
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(e) Separate the pistil from specimen C. cut the cross section of the ovary from specimen C and
obtain a thin layer, observe under low power of a microscope. Draw but only label parts that
participate in reproduction (10mks)

END
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