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Image J Tutorial: Chee 291 - Instrumentation and Measurement 1 - Fall 2015

Image J is an open source software that provides tools for analyzing and processing digital images. It can be downloaded from http://imagej.nih.gov/ij and its documentation is available on that website. The software allows users to set scales, create regions of interest using selection tools, make manual measurements by drawing lines and viewing results, perform automated thresholding to convert images to grayscale and adjust pixel selection, fill holes within objects, use watershed to separate touching objects, and analyze particles to characterize objects in the image.

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74 views

Image J Tutorial: Chee 291 - Instrumentation and Measurement 1 - Fall 2015

Image J is an open source software that provides tools for analyzing and processing digital images. It can be downloaded from http://imagej.nih.gov/ij and its documentation is available on that website. The software allows users to set scales, create regions of interest using selection tools, make manual measurements by drawing lines and viewing results, perform automated thresholding to convert images to grayscale and adjust pixel selection, fill holes within objects, use watershed to separate touching objects, and analyze particles to characterize objects in the image.

Uploaded by

razanmk961214
Copyright
© © All Rights Reserved
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd
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Image J Tutorial

CHEE 291 -INSTRUMENTATION AND MEASUREMENT


1 Fall 2015

Image J
An open source software which provides tools for
analysing and treating digital images
Download from this website: http://imagej.nih.gov/ij/
Documentation on Image Js different functions can be
found directly on that website: http
://imagej.nih.gov/ij/docs/guide/user-guide.pdf

Menu Bar

Image J Toolbar

Tool Bar

Status Bar

Setting the Scale

ROIs (Regions of Interest)


ROIs can be created by using the first few (left-most)
icons on the standard ImageJ toolbar, namely the area
selection tools:

Manual Measurements
The left-most tools can be used to make measurements. Use the line tool to draw one of the coins
diameter, then go to Analyze->measure. A pop-up window will contain various metric about the
line you just drew. As you draw more lines and repeat the list of results gets longer.

Automated Measurement
Automated measurement starts by converting the color image to a grey-scale image.

Thresholding
Simple thresholding marks all pixels with values less than the threshold as off or 0,
and all pixels with values equal to or above the threshold as on or 1. Move the sliders
up and down to adjust the selection, then click apply

Fill
Holes
misses some pixels due to

The thresholding
illumination. You can fill holes inside the coins.

reflection and uneven

Watershed

Without using watershed these two coins


would be treated as single particle in the
next step

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Analyze Particles
ImageJ kindly includes an analyze particles function since its so common

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Analyze Particles

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