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Signal Transduction Pathways: Pratt & Cornely, Chapter 10

Signal transduction pathways allow cells to respond to external signals. There are several key components: ligands bind to receptors, which activate transducers that produce second messengers, ultimately affecting target proteins or DNA through effectors. Common pathways involve G protein-coupled receptors, receptor tyrosine kinases, or intracellular steroid receptors. Second messengers like cAMP or IP3 activate cascades like protein kinase A or protein kinase C phosphorylation to regulate cellular responses. Turning pathways off at different points provides control and cross-talk between pathways allows integration of multiple signals.

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42 views

Signal Transduction Pathways: Pratt & Cornely, Chapter 10

Signal transduction pathways allow cells to respond to external signals. There are several key components: ligands bind to receptors, which activate transducers that produce second messengers, ultimately affecting target proteins or DNA through effectors. Common pathways involve G protein-coupled receptors, receptor tyrosine kinases, or intracellular steroid receptors. Second messengers like cAMP or IP3 activate cascades like protein kinase A or protein kinase C phosphorylation to regulate cellular responses. Turning pathways off at different points provides control and cross-talk between pathways allows integration of multiple signals.

Uploaded by

Brother George
Copyright
© © All Rights Reserved
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Download as PPTX, PDF, TXT or read online on Scribd
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Signal Transduction Pathways

Pratt & Cornely, Chapter 10


Terms for Signal Transduction
• Ligand
• Receptor
• Transducer
• Effector
• Second messenger
• Target proteins/DNA
Ligands
• Hormones vs Local mediators
• Polar (insulin) vs nonpolar (steroidal hormone)
• Specific—high affinity
• Agonist vs antagonist
Quantitative Ligand Binding
𝑅 [𝐿] 𝑅 [𝐿]
• KD values K= [𝑅𝐿]
and [RL] =
[𝑅𝐿]
𝐾
% bound =
• Problem 7: Substitute:
𝑅 +[𝑅𝐿]

𝑅 [𝐿]
Derive an % bound =
𝑅
𝐾
+ 𝐾
𝑅 [𝐿]
𝑅 [𝐿] [𝐿]
= 𝐾 𝑅 + 𝑅 [𝐿] = 𝐾+[𝐿]
expression for Hyperbolic function!

the [RL]/[R]T
ratio.
Scatchard Plot
• Problem 14: A Scatchard Plot is another
method of representing ligand binding data.
The slope is equal to -1/KD. Use the chart to
estimate KD for calmodulin binding to calcium.
G-Protein Signaling Pathways
• Use b-adrenergic receptor as example of G-
Protein Coupled Receptor (GPCR)
• 7-transmembrane (7-TM) receptor
G-Protein Coupled
• Ligand binding causes
G-protein to associate
with receptor (figure
not quite right)
• Three subunits, lipid
anchored
– a binds GDP
– b, g tightly associated
• Binding causes GDP
release
G-Protein Activation
• GTP binds
– Destabilized trimer
– Release each other
and receptor as two
active proteins
• Turn off: Slow GTP
hydrolysis
– Subunits reassemble
to inactive form until
they can bind
receptor again
cAMP
• G-protein carries signal to another protein
– transducer
• Adenylate cyclase
– effector
• Catalyzes formation of cAMP
– second messenger
• Amplification
Protein Kinase A
• cAMP acts as second
messenger to activate
Protein Kinase A
(allosteric activator)
• Regulatory and catalytic
subunits
Phosphorylation
• Common activation/
deactivation strategy
• Changes protein
conformation
drastically
• Covalent
modification
• Middle range time
effect
Protein Kinase A
• PKA modulates the activity of
enzymes that carry out work
through phosphorylation
• For example, adrenaline
binding leads to PKA activating
the enzyme that releases
glucose from storage to be
used
• Exercise: use basic guide to
explain mechanism of
epinephrine affect on sugar
release in muscle
Turning Off Pathway
• Can turn it off at any point
– Receptor?
– G-protein?
– Second messenger?
– Phosphorylated enzyme?
Phosphinositol Pathway
• Many G-Proteins for many
pathways
– Cross-talk—different
paths give same result
• a-adrenergic receptor
(liver but not muscle)
– Same hormone gives
different responses
– Liver also has glucagon
binding, so a-receptor
allows for fine-tuning of
signal
– Target of this G-protein is
phospholipase C
Two second Messengers

• PIP2  IP3
– Opens Calcium gates
• Activates Protein Kinase B (Akt) to make other second
messengers
• PIP2  DAG
– Activates Protein Kinase C
• Also requires Ca+2
• Especially important in cell division
Receptor Tyrosine Kinases
• Second major class of receptors
– Insulin binding as prototype
– Mostly monomers that bind ligand and then
dimerize
• One subunit binds ligand
• Second subunit become active kinases
Insulin Signaling
Other Receptor Tyrosine Kinases
• Target nuclear proteins
Oncogenes
• Ras targets nuclear proteins
• Key signal in cell growth
• Problem 46: Mutant Ras proteins have been
found to be associated with various types of
cancer. What is the effect on a cell if the
mutant Ras is able to bind GRP but is unable
to hydrolyze it?
Lipid Hormone Signaling

• Cortisol binds intracellular Zinc finger


• Dimerization binds hormone response
element
• Transcription factor—activate or inhibit
• Steroidal anti-inflammatory
Problem 53
• Steroid hormone receptors have different
cellular locations. The progesterone receptor
is located in the nucleus and interacts with
DNA once progesterone has bound. But the
glucocorticoid receptor is located in the
cytolol and does not move into the nucleus
until its ligand is bound. What structural
feature must be different in these two
receptor molecules?
Local Mediators
• Eicosanoids
produced in
response to cellular
event
• Produce hormone-
like responses in
blood pressure,
inflammation
response, etc.
COX Targets
• NSAIDs (non-steroidal anti-inflammatory
drugs) target cyclooxygenase
• Aspirin, ibuprofen: COX 1 and 2
• Vioxx: COX 2
• Acetominophen: COX:3
– Less side effects
– Worse toxicity

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