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Histology Lecture 1, Introduction (Slides)

This document discusses histology methods for studying tissues. It outlines the key steps of tissue preparation - fixation using chemicals like formaldehyde to preserve structure, embedding and sectioning tissues thinly using a microtome, and staining using dyes like hematoxylin and eosin to make structures visible. These processing methods allow examination of tissues under light microscopes due to cells and components being too small to see otherwise.

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Ali Al-Qudsi
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© Attribution Non-Commercial (BY-NC)
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Download as PPT, PDF, TXT or read online on Scribd
1K views

Histology Lecture 1, Introduction (Slides)

This document discusses histology methods for studying tissues. It outlines the key steps of tissue preparation - fixation using chemicals like formaldehyde to preserve structure, embedding and sectioning tissues thinly using a microtome, and staining using dyes like hematoxylin and eosin to make structures visible. These processing methods allow examination of tissues under light microscopes due to cells and components being too small to see otherwise.

Uploaded by

Ali Al-Qudsi
Copyright
© Attribution Non-Commercial (BY-NC)
Available Formats
Download as PPT, PDF, TXT or read online on Scribd
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Histology Methods of Study

Dr. Nour Erekat, PhD


Histology
• The study of the body tissues and how
they are arranged to constitute organs
– Dependent on the use of microscopes
• Due to the small size of cells and matrix
components
Tissue Preparation for Histology
• Steps for tissue preparation
1- Fixation
2- Embedding and sectioning
3- Staining
Fixation
• Aim
– Preserve the structure and molecular
composition of the tissue
• By avoiding tissue digestion
• Methods
1- Physical (e.g. Freezing)
2- Chemical
- Using fixatives (e.g. 37% formaldehyde)
Embedding and Sectioning
• Embedding
– Aim
• Facilitate sectioning
– Embedding materials
• Paraffin for light microscopy
• Resins for both light and electron microscopy
• Sectioning
– Microtome
• 1-10 µm (1-10 micrometers)
Staining
• Aim
– Make tissue components conspicuous and distinctive
• Using dyes
1- Basic
- Stain basophilic tissue components with a net negative
charge (anionic)
- Hematoxylin
2- Acid
- Stain acidophilic tissue components (cationic)
- Eosin
Staining
Microscopy
• Classification
1- Electron microscopy
A- Transmission
B- Scanning
2- Light microscopy
 Bright-field microscopy
 Components
1. Optical
2. Mechanical
Bright-Field Microscopy
• Optical components
1. Condenser lens
2. Objective lenses
• Quality determines resolving power (0.2 µm)
– Quality of image
– Objective lenses of higher magnification have higher
resolving power
3. Ocular (eyepiece) lens
Bright-Field Microscopy

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