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Charles Zuwu Baysah (查尔斯) R3 - 6218000145

The document reviews a study that investigated photo-crosslinked poly(ethylene glycol) diacrylate (PEGDA) hydrogels for use in drug delivery applications. PEGDA hydrogels were synthesized using UV irradiation. Testing showed the hydrogels had swelling ratios of 7-8 times their dry weight in water. Swelling decreased with increasing ionic concentration. Glucose and insulin permeability tests indicated the hydrogels could sustain delivery of small drugs. Further testing of biocompatibility is still needed.

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76 views16 pages

Charles Zuwu Baysah (查尔斯) R3 - 6218000145

The document reviews a study that investigated photo-crosslinked poly(ethylene glycol) diacrylate (PEGDA) hydrogels for use in drug delivery applications. PEGDA hydrogels were synthesized using UV irradiation. Testing showed the hydrogels had swelling ratios of 7-8 times their dry weight in water. Swelling decreased with increasing ionic concentration. Glucose and insulin permeability tests indicated the hydrogels could sustain delivery of small drugs. Further testing of biocompatibility is still needed.

Uploaded by

Charles Zuwu Baysah
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© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Photo-crosslinked Poly(ethylene Glycol) Diacrylate (PEGDA)

Hydrogels From Low Molecular Weight Prepolymer:


Swelling And Permeation Studies (A Review)
(By Anna Cavallo et al)
CHARLES ZUWU BAYSAH - 6218000145
Table of Contents
 Background and Introduction
 Experimentation
 Results and Discussion
 Conclusion
Background/Introduction
 Medical science has improved significantly over the past decades; however, there still exist lots of
challenges with new ones emerging also. Ex. Infectious diseases (HIV/AIDS, Hepatitis, etc),
Cancer, etc.
 HIV/AIDS -  72mil infected since 1980s; 35mil death; 36.9mil positive world wide.
 Cancer – 9.6mil estimated to have died in 2017; Hepatitis – 257mil positive,  880,000 death/yr.

 Now, what does these numbers suggest?


- they remain a major global challenge.
Drug Delivery

 Drug Delivery refers to approaches, formulations, technologies, and systems for transporting a
pharmaceutical compound in the body as needed to safely achieve its desired therapeutic effect.
 Pharmaceuticals are either encapsulated or immerged in the delivery vehicle.
 Functions: prevent premature (burst) release of pharmaceutics, move pharmaceuticals across biological
barriers, reduce toxicity, effect the release of pharmaceuticals on disease cells.
 Many delivery substances have been made and are in use; however, the need for delivery technologies
for existing diseases and emerging ones remains a challenge.
 This indeed is an active research field where several nanoparticles are being investigated for possible
application. The paper reviewed investigated a Polyethylene glycol diacrylate (PEGDA) hydrogel with
focus on it swelling and permeation behavior.
 Hydrogels are three-dimensional polymer networks formed by crosslinked hydrophilic polymer
chains.

 Properties:
- Ability to absorb and retain water.
Due to this property, hydrogels are often used in regenerative medicine
and tissue engineering as soft tissue-like scaffolds to allow cell attachment,
growth and differentiation.
- Have tunable mechanical and diffusive properties, dependent on the
crosslink density.
Makes them optimal tools for drug delivery and cell encapsulation
Experimentation
 Hydrogel Synthesis
 Poly(ethylene glycol) diacrylate (PEGDA) (MW 700 Da) was first dissolved in
distilled water at 10 w/v % concentration.
 The photoinitiator Darocur 1173 was added to the PEGDA precursor solution at
0.3 w/v % concentration.
 The resulting solution was polymerized in Petri dishes (35 mm diameter)
through UV irradiation (365 nm at 2 mW/cm) for different time lengths (1, 2,
and 3 min).

Petri Dish
Swelling Test in Water
 From the three obtained samples, 3 circular samples (8mm) were punched out.
 These samples were then kept in water for 24hr at 25°C for removal of unreacted monomers
and solvent and for swelling test.
 Samples were removed from the water and weighed; then dried at 35°C, and weighed again.
 The swelling capability of the gels was expressed as mass swelling ratio, Q.

 Knowing the polymer density, ρ (1.1g/cm3) and density of water, ρH2O, the polymer volume
fraction can be obtained from Q.
Swelling Measurement in Aqueous Solutions with Different Ionic Strength and pH

 Swelling ratio was also measured in solutions containing different ionic strength and
pH at 37°C
 Selected quantities of NaCl was dissolved to form solutions with different ionic
strength (0 – 300mM).
 Hydrogel mesh size was then calculated by using the Q and the values of Mc gotten
form the distilled H2O testing.
Ussing Chamber Diffusion Experiment
 The permeation of the gels was tested for using glucose and insulin as solute.
 PEGDA hydrogels with thickness 0 – 0.3nm were incubated for 30min in 7.4pH solution and
then mounted between the chamber’s compartments with diffusion cross section area of
19.64mm2.
 Each compartment was filled with DPBS (7.4pH) solution. For glucose, 1.8mg/mL solution;
insulin, 0.2mg/mL.
 The experiments were done for each sample and the average taken; then the permeability
coefficient were calculated using Colton’s theory equation.

 For permeability, P, the slope of t graph was used to obtain, slope (m)

2𝐴𝑃
𝑚=
𝑉
Result and Discussion
Swelling in water
 Swelling ratio about 7 – 8;
Swelling in Ionic Medium
 Swelling ratio decreased with increase in ionic concentration. Hydrogels formed from
1min of irradiation were affected the most.
Effect of pH on Swelling
Glucose Permeability
Insulin Permeability
Conclusion
 Ionic strength had mor impact on swelling than pH
 The hydrogel crosslink density can be controlled by the time length of the UV
irradiation/rosslinking
 The mesh size was retained between 2 – 3nm through and after testing (compatible with the
diffusion of small drug)

The reported data suggest the as synthesized hydrogels have potential for sustained drug
delivery of small drugs

However, further testing needs to be done to investigate it biocompatibility and functionality.

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