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Exp 2 Disso Test

Here are the key steps to solve this problem: - Absorbance of sample = 0.725 - Absorbance of standard = 0.775 - Weight of powdered tablets = 0.015g - To convert grains to mg: 1 grain = 65mg - Labeled claim per tablet = 5 grains = 5 * 65mg = 325mg Calculate phenacetin content: Content of standard (mg/mL) x Absorbance of sample x Dilution Factor x Weight of powdered tablets Absorbance of standard Plug in values: x mg/mL x 0.725 x Dilution Factor x 0.015g 0.775 Solve for x and

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Edrian Villegas
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2K views

Exp 2 Disso Test

Here are the key steps to solve this problem: - Absorbance of sample = 0.725 - Absorbance of standard = 0.775 - Weight of powdered tablets = 0.015g - To convert grains to mg: 1 grain = 65mg - Labeled claim per tablet = 5 grains = 5 * 65mg = 325mg Calculate phenacetin content: Content of standard (mg/mL) x Absorbance of sample x Dilution Factor x Weight of powdered tablets Absorbance of standard Plug in values: x mg/mL x 0.725 x Dilution Factor x 0.015g 0.775 Solve for x and

Uploaded by

Edrian Villegas
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PPTX, PDF, TXT or read online on Scribd
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Exercise No.

2 & 3

•DISSOLUTION TEST
•Apparatus 1&2
• (Basket Assembly & Paddle Assembly)
EXERCISE NO.2
DISSOLUTION TEST OF AMOXICILLIN CAPSULES USING THE
ROTATING BASKET ASSEMBLY (Apparatus 1)

• Results and Analysis


Table of Tabulation and Results:
Absorbance reading of the RS (As) 2.730

Concentration of the RS (ug/mL) 10 ug/mL


Dilution factor 50,000

Weight of the 8 capsules 3.46 g

Average weight per capsule 0.4325 g


EXERCISE NO.2
DISSOLUTION TEST OF AMOXICILLIN CAPSULES USING THE
ROTATING BASKET ASSEMBLY (Apparatus 1)
• Results and Analysis
Capsule Au Amount % Labeled
Actual wt.
# Readings obtained claim

1 420 mg 2.749 518.46 mg 103.69%

2 460 mg 2.732 470.45 mg 94.09%

3 430 mg 2.799 515.62 mg 103.12%

4 450 mg 2.700 492.70 mg 98.54%

5 440 mg 2.31 491.66 mg 98.33%

6 400 mg 2.733 541.22 mg 108.24%


EXERCISE NO.2
DISSOLUTION TEST OF AMOXICILLIN CAPSULES USING THE
ROTATING BASKET ASSEMBLY (Apparatus 1)
• Computation

Aliquote :
500mg : 900mL :: 0.5mg : X
900𝑚𝐿 (0.5𝑚𝑔)
x= = 0.9𝑚𝐿
500𝑚𝑔

Dissolution Factor :
900𝑚𝐿 50
D. F. = 𝑥 = 50,000
0.9𝑚𝐿 1
EXERCISE NO.2
DISSOLUTION TEST OF AMOXICILLIN CAPSULES USING THE
ROTATING BASKET ASSEMBLY (Apparatus 1)
• Computation

Formula:
𝐴𝑈 𝐶𝑜𝑛𝑐.𝑜𝑓 𝑆𝑡𝑛𝑑 (𝑢𝑔 \𝑚𝐿 )
𝐴𝑠
𝑥 1000
𝑥 𝐷.𝐹.𝑥 𝐴𝑣𝑒.𝑤𝑡.\cap.
m𝑔/𝑐𝑎𝑝 =
𝑤𝑡 𝑜𝑓 𝑡ℎ𝑒 𝐶𝑎𝑝𝑠𝑢𝑙𝑒 𝑖𝑛 𝑚𝑔

%Label Claim:
𝐴𝑐𝑡𝑢𝑎𝑙 𝑤𝑒𝑖𝑔ℎ𝑡
%LC = 𝑥100
𝐿𝑎𝑏𝑒𝑙𝑒𝑑 𝑐𝑙𝑎𝑖𝑚
• In the dissolution test for capsules, why
is it necessary to use the cylindrical
basket instead of the paddle?

• The pores in the mesh allow the dissolving drug to move


from the basket into the holding vessel, creating a
uniform solution.
• The sticky tablets/capsules containing HPMC or similar
compounds as a binder are tested using a basket
because tablet swells in water & may stick to the walls
of the dissolution vessel.
Q&A
• Why is the sampling procedure done at
a point midway between the tip of the
basket and the surface of the
dissolution medium?

• It is where the concentration is exact. The sample at the


bottom of the vessel is at is concentrated state while at
the top is diluted.

Q&A
• In tabulated form, present the different
apparatus numbers and the
description/names of each.
Apparatus Name an Decription
1 Rotating Basket
2 Paddle
3 Reciprocating Paddle
4 Flow cell
5 Paddle over disk
6 Cylinder
7 Recipprocating Disk
8 Non-USP NF
9 Non-USP NF

Q&A 10 Non-USP NF
EXERCISE NO.3
DISSOLUTION TEST OF AMOXICILLIN CAPSULES USING THE
ROTATING PADDLE ASSEMBLY (Apparatus 2)

• Results and Analysis


Table of Tabulation and Results:
Absorbance reading of the RS (As)

Concentration of the RS (ug/mL) 1.38


Dilution factor 32,608.70

Weight of the 10 capsules

Average weight per capsule 0.3583g


EXERCISE NO.3
DISSOLUTION TEST OF AMOXICILLIN CAPSULES USING THE
ROTATING PADDLE ASSEMBLY (Apparatus 2)
• Results and Analysis
Capsul Time Au Amount % Labeled
Actual wt.
e# dissolved Readings obtained claim

1 76 0.3565g

2 89 0.3555g

3 85 0.3551g

4 78 0.3573g

5 39 0.3576g

6 32 0.3572g
EXERCISE NO.3
DISSOLUTION TEST OF AMOXICILLIN CAPSULES USING THE
ROTATING PADDLE ASSEMBLY (Apparatus 2)
• Computation

Aliquot :
325mg : 900mL :: 0.5mg : X
900𝑚𝐿 (0.5𝑚𝑔)
x= = 1.38𝑚𝐿
325𝑚𝑔

Dissolution Factor :
900𝑚𝐿 50
D. F. = 𝑥 = 32,608.70
1.38𝑚𝐿 1
EXERCISE NO.3
DISSOLUTION TEST OF AMOXICILLIN CAPSULES USING THE
ROTATING PADDLE ASSEMBLY (Apparatus 2)
• Computation

Formula:
𝐴𝑈 𝐶𝑜𝑛𝑐.𝑜𝑓 𝑆𝑡𝑛𝑑 (𝑢𝑔 \𝑚𝐿 )
𝐴𝑠
𝑥 1000
𝑥 𝐷.𝐹.𝑥 𝐴𝑣𝑒.𝑤𝑡.\cap.
m𝑔/𝑐𝑎𝑝 =
𝑤𝑡 𝑜𝑓 𝑡ℎ𝑒 𝐶𝑎𝑝𝑠𝑢𝑙𝑒 𝑖𝑛 𝑚𝑔

%Label Claim:
𝐴𝑐𝑡𝑢𝑎𝑙 𝑤𝑒𝑖𝑔ℎ𝑡
%LC = 𝑥100
𝐿𝑎𝑏𝑒𝑙𝑒𝑑 𝑐𝑙𝑎𝑖𝑚
• What ranges of wavelengths
correspond to the ultraviolet and visible
regions of the electromagnetic
spectrum?

Q&A
• In dissolution test for aspirin tablets,
why is it necessary to use the paddle
instead of the rotating basket
assembly?

Q&A
• What is the aliquot portion equivalent
to 0.5mg aspirin and thee dilution
factor to be in the determination of
aspirin content per capsule, if one
capsule of 600mg has been dropped in
900mL of a medium and diluted to
50mL?

Q&A
• Calculate the Phenacetin content in
tablets labeled as 5 grains if the
Absorbance of the sample was 0.725,
while that of the standard was 0.775.
the weight of the powdered tablets was
0.015g. Did results meet the labeled
claim of 5 grains per tablet?

Q&A

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