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Biochemical Engineering Problems

The document contains experimental data measuring the flow rate, cell concentration, and substrate concentration for a bioreactor. It then uses this data to construct a Lineweaver-Burk plot to determine the maximum reaction rate and Michaelis constant for the system.

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Ryle Arbon
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52 views

Biochemical Engineering Problems

The document contains experimental data measuring the flow rate, cell concentration, and substrate concentration for a bioreactor. It then uses this data to construct a Lineweaver-Burk plot to determine the maximum reaction rate and Michaelis constant for the system.

Uploaded by

Ryle Arbon
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd
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𝑽 = 𝟎.

𝟎𝟏𝟐𝟗 𝑳
Flow rate,F Cell conc., X Substrate Conc.
(mL/h) (g/L) S (g/L)
31 5.97 0.5
50 5.94 1.0
71 5.88 2.0
91 5.76 4.0
200 0 100
F V/F Cs 1/Cs

0.031 16.129 0.5 2

0.05 10.000 1.0 1

0.071 7.042 2.0 0.5

0.091 5.495 4.0 0.25

0.2 2.500 100 0.01


Lineweaver-Burk Plot
18.000

16.000

14.000

12.000

10.000
V/F

8.000
y = 6.0681x + 3.9776
R² = 1
6.000

4.000

2.000

0.000
0 0.5 1 1.5 2 2.5
1/Cs
𝟎.𝟐𝟓𝟏𝑪𝒔
rx =
𝟏.𝟓𝟐+𝑪𝒔
950 L
192 L

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