DXH 600/800

HARDWARE

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 MODULES
• Aspiration
• CBC
• VCSn
• Data plot Development
• Common Services
• Specimen Transport

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 Here is how to navigate this pro

ASPIRATION
MODULE

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OBJECTIVES:

›Sample Aspiration Module

›Aspiration Syringe Pump Assembly
›Blood Sampling Valve Assembly
›Blood Detectors

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 SAMPLE ASPIRATION MODULE
• SAMPLE ACQUISITION CYCLE
• 2 sub-cycles
a. Aspiration sub- cycle
1. Air Gap
2. Venting
b. Transport Sub cycle
c. Blood Detection

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SAMPLE ACQUISITION

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 Aspiration Syringe Pump Assembly

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ASPIRATION SYRINGE PUMP

• The Aspiration Syringe Pump Module

is controlled by the Steps of a
Stepper Motor
• “Encoder”
• Keeps track of the motor steps
• Each motor step equals a
certain volume of fluid
(i.e. 1µL = 6 steps)
• The speed of the motor in steps
per second controls how fast
the fluids move

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 Blood Sampling Valve Module

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BLOOD SAMPLING VALVE MODULE

• Blood Sampling Valve

Module:
• Blood Sampling Valve (BSV)
• Controls the pathways for
blood delivery for both CBC
& VCSn Analysis
• BSV Motor
• Moves the center section of
the BSV
• BSV Sensors
• Monitor the position of the
center section of the BSV
• BSV Animation
• BSV Animation

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 Blood Detectors

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 BLOOD DETECTORS

• Blood Detectors Monitor the

quality of aspiration
• Probe Blood Detector
• Aspiration Sub-Cycle
• First part of
Transport Sub-Cycle
• BSV Blood Detector
• Transport Sub-Cycle

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BLOOD DETECTOR

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SAMPLE ACQUISITION SUMMARY:
In summary:
1. Air Gap
2. Probe pierces tube and vents
3. Aspirate 165µL blood
4. Blood thru Probe Blood
Detector, stops
5. Transport begins
6. Blood pulled thru BSV then
BSV Blood Detector, stops
7. BSV segments, CBC blood is
pushed to CBC baths
8. Blood pushed back into
Probe, NRBC blood dispense
9. BSV home, DIFF & RETIC
blood dispense
10. Backwash and clean probe

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 Here is how to navigate this pro

CBC
MODULE

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CBC TRANSDUCER PANEL

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CBC TRANSDUCER PANEL

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CBC WASTE/VENT ASSEMBLY

CBC Waste Chamber (VC115):

• Waste from CBC Baths,
Count Chambers, HGB
Cuvette
• Supplies Diluent/CBC Lyse
during “Shutdown/Remove
Cleaner”
Vent/Overflow Chamber (VC199):
• Supplies Vent for-
• CBC Waste, CBC Baths,
HGB Cuvette, Diluent
Buffer & Feeder, VCSn
Flow Cell Waste (VC218)

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 CBC Module
• CBC Waste Management
• VC115 (CBC Waste Chamber)
• VC199 (Vent/Overflow Chamber)

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 CBC Module
• WBC Correction:
• UWBC (uncorrected)
• WBC (corrected)
• Data from WBC Count, VCSn Analysis, WBC Histogram
• Proprietary Advanced Algorithms
• Digital pulse processing provides increased
discrimination using cell pulse characteristics
• Advanced UniCel DxH 800 CBC algorithms utilize
additional cellular measurements for:
• Elevated WBC
• Improved WBC
• Enhanced detection capabilities for identification
of cellular carryover and interferences
• Interference correction is performed
automatically

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 HISTOGRAM REVIEW

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WBC Histogram

 X-Axis Lymphocytes
• Size Channels (0-450 fL)
• Most interference occurs at
the 35 fL threshold Granulocytes
 Y-Axis
• Relative number (# of Mononuclear
events)
 3 WBC Populations
Displayed:
• Lymphocytes (35-90 fL) LY GR
MO
• Mononuclear (90-160 fL)
• Granulocytes (160-450 fL)

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PLT Histogram

› See platelet monograph Sample Fitted Curve

Preparation for more info:
DxH 600/800 Platelet Methodology

› Two Platelet Histograms:

» Raw data pulses in the size
range of 2-25 fL counted as Raw Data Curve
platelets
» Normal results- only the
“fitted curve” is displayed
» Abnormal results- only the
“raw data” curve is displayed

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 Here is how to navigate this pro

VCSn
MODULE

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 VCSn MODULE

A. Sample Flow
• Air Mix Temperature Control Module (AMTC)
• Sample Conditioning (Preparation)
• Distribution Valve (DV)
• Sheath/Sample Pressure
• Gains and Phase

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VCSn MODULE

AMTC

Nice and CLEAN!!!!

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VCSn MODULE

• Distribution Valve (DV)

• DV
• Stepper motor
• Rotates 360o
• 9 positions
• Dual Sensor
• Code Wheel
• DV Function
• Provides Pathways
• Position Sample in sample
line
• Clean pathways
• Remove waste
• Cleaning grooves
• 24 hour auto-cycle

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DISTRIBUTION VALVE (DV)

Distribution Valve
• Common Problems:
• Freeze up
• Symptom:
• Loud grinding
• DV error messages
• Cause:
• Excessive build up
diluent/cleaner

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VCSn MODULE

Distribution Valve (DV)

• Common Problems (cont’d):
• Counter measures
• Dx Tools
• Cycle DV- verifies the
DV motor, clutch, and
optical sensors (right
10 times, then left)
• Unlock DV- helps free
the distribution valve
when it is frozen and
cannot rotate
• DI H2O
• Send service

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VCSn MODULE

• Sample/Sheath Pressure
• Sheath = 8.00 psi (7.98-8.02)
• Sample = typical 8.9-9.2 psi
(nominal = 9.1 psi)
• Advisor Case #K96323234
• Sheath tank
• Sample tank
• Hydrodynamic Focusing
• Processes by which
individual cells or particles
are focused in a stream of
diluent. Cells are aligned
and pass through the flow
cell in a singular fashion
Right Side of Instrument

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VCSn MODULE

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VCSn MODULE

• Gains/Phase
• Initial Gains/Phase
(DxH 800 FSM 4.200.20)
• Final Gains/Phase
(DxH 800 FSM 4.200.23)
WHY?
• Temperature
Compensation

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 VCS MODULE

B. Data Plot Development

• Thresholds (ET & SW)
• Impedance
• Light Analysis
• Populations

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VCSn MODULE

Data Plot Development

• DF1
• Volume (impedance, 256
channels)
• MALS (median angle light
scatter, 256 channels)
• Two thresholds:
• Electronic (ET)
• Software (ST) ET
• Electronic (ET)
• Fixed impedance – total
events tracking (8192)

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VCSn MODULE

Data Plot Development (cont’d)

• Software Threshold (ST)
• Discriminates WBCs from
Non-WBCs
• Everything above = WBCs
• Below to ET = Non-WBCs

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VCSn MODULE

Data Plot Development (cont’d)

• The five WBC Differential
populations are:
• EO- Eosinophils: EO’s are a minor
MO
population and may not appear on NE EO
the scatterplot.
• NE- Neutrophils BA
LY
• MO- Monocytes
• LY- Lymphocytes
• BA- Basophils (behind the
Lymphs)

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VCSn MODULE

NRBC Data Plot

• LALS (low angle light scatter)
• AL2 (axial light loss)
Grans
• Absorbance Debris

• Debris/Abnormal Platelets
LY

• Lymphocytes
• Granulocytes (MO, NE, EO)
• NRBCs
NRBCs

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 Here is how to navigate this pro

COMMON
SERVICES
MODULE

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 COMMON SERVICES

PNEUMATIC SERVICES MODULE

• Pneumatic Supply
Module (PSM)
• Raw Pressure (25 psi)
• Raw Vacuum (25 Hg
vacuum)
• Regulators
• 4 psi (mix pressure)
• 25 psi (Raw Pressure)
• Sample pressure
• Sheath pressure
• Low vacuum (6 Hg
vacuum)

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 COMMON SERVICES

PNEUMATIC SERVICES MODULE

• Pneumatic Supply
Module (PSM)
• Manual PSM Power Up
(3 ways)
1. Dx Tools Maintenance
tab (Start Pneumatics)
2. System Monitor
VL303
3. PSM bypass switch on
control board under
top cover

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COMMON SERVICES

PNEUMATIC SERVICES MODULE

• 4 psi regulator (VCSn mix
pressure)

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 Common Services Module
• Reagent Services Module

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REAGENT SERVICES
MODULE REAGENT DETECTION
• Reagent Detection
• Sensing Electrode in buffer/reservoir
• Cycle Counter
• “Configure Low Level” (yellow alert), no
effect in/on determination of reagent
depletion
• Reagent Depleted
• Non-Diluent Reagent
• unable to fill the reagent buffer
within timeout
• Cycles typically 0, but not always
• All diluent supplies are depleted means
that both diluent supplies were consumed
logically (the counters decremented to 0 and
the diluent buffer always filled on time)
• Unable to sense diluent means that the
diluent buffer wasn't able to fill within the
expected timeout and most likely the diluent
manifold has ingested air

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REAGENT SERVICES
MODULE
DILUENT NOT SWITCHING
• Advisor case #K13237613
• Failure modes
• Pick up tube
• QD leaking/not connected
• Input tubing kinked
• Rescanned after software
update
• Operator error
• Not replacing when
depleted
• Pooling

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REAGENT SERVICES
MODULE

DILUENT NOT SWITCHING

• Failure modes (cont’d)
• Over consumption
• Diluent Threshold adjustment
Advisor case #K45444431
• 420 ml <200
specimens/day
• 530 ml > 200
specimens/day
• Threshold function
• Sets level at which diluent
switching occurs
• Dead volume

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 Here is how to navigate this pro
SPECIMEN
TRANSPORT
MODULE
(STM)

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 Specimen Transport Module (STM)
• Cassette Flow
• Mixer

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SPECIMEN TRANSPORT MODULE (STM)

Cassette Detection Sensor Locations- Advisor Case #K32701675

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SPECIMEN TRANSPORT MODULE (STM)

Mixer Station
• Failure Modes
• Stuck
• Counter Measure- Advisor
Case #K32701675
• Test sensor function
• Clean Home Sensor with
DI H2O

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 REFERENCES:
• UniCel DxH
Hematology Analytical
Stations Field Service
Manual, Rev AL
• DxH Connectivity
Workstation Manual
(Airwatch)
• Advisor Cases

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THE END
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