BM800 Flow diagram

General description of flowchart

 Ready for analysis

SPa SPb

CH1

V28

M1 Dis1 Dis2 M2 V5 V6 V7 V8 V9
Shear valve Pos1 V1 V2 V3 V4

6
5 7
Air pumpP+
4
BloodD 8
V10 V11
3
9
CH2

2
10 MC1 MC2
11
BeakerD
1
V27 MPA
V12
V13 V14
PM

OT PD V15 R1

Wash cup V16 V17 V18 V19

V23 V24 V25 V26 UVacuumP-
V20 V21 V22 MV-Dil
Diluent
R2 Lyse
R3 MV-Lys

LVacuumP-

 Initial state with 4 pipettes full, 2 measuring chamber filled

2
 Whole blood aspiration (open tube)

SPa SPb

CH1

V28

M1 Dis1 Dis2 M2 V5 V6 V7 V8 V9
Shear valve Pos1 V1 V2 V3 V4

6
5 7
Air pumpP+
4
BloodD 8
V10 V11
3
9
CH2

2
10 MC1 MC2
11
BeakerD
1
V27 MPA
V12
V13 V14
PM

OT PD V15 R1

Wash cup V16 V17 V18 V19

V23 V24 V25 V26 UVacuumP-
V20 V21 V22 MV-Dil
Diluent
R2 Lyse
R3 MV-Lys

LVacuumP-

 Lower P- aspirates blood sample via V27, SV1-3, Process terminated by blood detector
 P- aspirates from wash cup via V14 in the same time 3
 Prepare for first dilution

SPa SPb

CH1

V28

M1 Dis1 Dis2 M2 V5 V6 V7 V8 V9
Shear valve Pos2 V1 V2 V3 V4

6
5 7
Air pumpP+
4
BloodD 8
V10 V11
3
9
CH2

2
10 MC1 MC2
11
BeakerD
1
V27 MPA
V12
V13 V14
PM

OT PD V15 R1

Wash cup V16 V17 V18 V19

V23 V24 V25 V26 UVacuumP-
V20 V21 V22 MV-Dil
Diluent
R2 Lyse
R3 MV-Lys

LVacuumP-

 Shear valve turns to Pos2, blood sample moves to SV2-4.

4
 First dilution

SPa SPb

CH1

V28

M1 Dis1 Dis2 M2 V5 V6 V7 V8 V9
Shear valve Pos2 V1 V2 V3 V4

6
5 7
Air pumpP+
4
BloodD 8
V10 V11
4
3 3
9
CH2

2
10 MC1 MC2
1 11
V27 MPA
V12
V13 V14
PM

OT PD
V15 R1

Wash cup V16 V17 V18 V19

V23 V24 V25 V26 UVacuumP-
V20 V21 V22
Diluent
MV-Dil
R2 Lyse
R3 MV-Lyz

LVacuumP-

 P+ pushes 4.5 ml dil from Dis1 to PM via V9, V17, SV2-4

 P- Empties both RBC and WBC chambers via CH2, V11, V10, MC1 air vent via V3, MC2 via V4, V28 5
 Mix in PM beaker

SPa SPb

CH1

V28

M1 Dis1 Dis2 M2 V5 V6 V7 V8 V9
Shear valve Pos1 V1 V2 V3 V4

6
5 7
Air pumpP+
4
BloodD 8
V10 V11
3
9
CH2

2
10 MC1 MC2
11
BeakerD
1
V27 MPA
V12
V13 V14
PM

OT PD V15 R1

Wash cup V16 V17 V18 V19

V23 V24 V25 V26 UVacuumP-
V20 V21 V22 MV-Dil
Diluent
R2 Lyse
R3 MV-Lys

LVacuumP-

 P- creates vacuum in PM by loading SP via CH2, V5, CH1, V7, V4

 Air gets in PM and bubble-mix via V1, MPA and PD needle 6
 Prepare for pipette wash

SPa SPb

CH1

V28

M1 Dis1 Dis2 M2 V5 V6 V7 V8 V9
Shear valve Pos1 V1 V2 V3 V4

6
5 7
Air pumpP+
4
BloodD 8
V10 V11
3
9
CH2

2
10 MC1 MC2
11
BeakerD
1
V27 MPA
V12
V13 V14
PM

OT PD V15 R1

Wash cup V16 V17 V18 V19

V23 V24 V25 V26 UVacuumP-
V20 V21 V22 MV-Dil
Diluent
R2 Lyse
R3 MV-Lys

LVacuumP-

 Upper part P- aspirates dil to Dis1 via V-dil, V22, CH1, V5, CH2 by timing
7
 Pipette wash

SPa SPb

CH1

V28

M1 Dis1 Dis2 M2 V5 V6 V7 V8 V9
Shear valve Pos1 V1 V2 V3 V4

6
5 7
Air pumpP+
4
BloodD 8
V10 V11
3
9
CH2

2
10 MC1 MC2
11
BeakerD
1
V27 MPA
V12
V13 V14
PM

OT PD V15 R1

Wash cup V16 V17 V18 V19

V23 V24 V25 V26 UVacuumP-
V20 V21 V22 MV-Dil
Diluent
R2 Lyse
R3 MV-Lys

LVacuumP-

 P+ pushes dil from Dis1 to washing device via V9, CH1, V18, SV11-6, outside of needle
 Lower part P- collects waste from inside needle via SV1-3, V27 and from wash cup via V14 8
 Fill diluent pipette

SPa SPb

CH1

V28

M1 Dis1 Dis2 M2 V5 V6 V7 V8 V9
Shear valve Pos1 V1 V2 V3 V4

6
5 7
Air pumpP+
4
BloodD 8
V10 V11
3
9
CH2

2
10 MC1 MC2
11
BeakerD
1
V27 MPA
V12
V13 V14
PM

OT PD V15 R1

Wash cup V16 V17 V18 V19

V23 V24 V25 V26 UVacuumP-
V20 V21 V22 MV-Dil
Diluent
R2 Lyse
R3 MV-Lys

LVacuumP-

 P- fills Dis1 via CH2, V5 CH1, V22 and MV-Dil

 P- charges SP to create vacuum in MC1 and MC2 9
 Empty measuring pipettes

SPa SPb

CH1

V28

M1 Dis1 Dis2 M2 V5 V6 V7 V8 V9
Shear valve Pos1 V1 V2 V3 V4

6
5 7
Air pumpP+
4
BloodD 8
V10 V11
3
9
CH2

2
10 MC1 MC2
11
BeakerD
1
V27 MPA
V12
V13 V14
PM

OT PD V15 R1

Wash cup V16 V17 V18 V19

V23 V24 V25 V26 UVacuumP-
V20 V21 V22 MV-Dil
Diluent
R2 Lyse
R3 MV-Lys

LVacuumP-

 P- empties M1 via V15, MC1 bypass, V12, Spa, V7, CH1, V5, CH2
 P- empties M2 via V26, MC2 bypass, V13, SPb, V7, CH1, V5, CH2 10
 Prepare for second dilution

SPa SPb

CH1

V28

M1 Dis1 Dis2 M2 V5 V6 V7 V8 V9
Shear valve Pos1 V1 V2 V3 V4

6 T1
5 7
Air pumpP+
4 8
V10 V11
BloodD
3
9
CH2

2
10 MC1 MC2
11
BeakerD
1
V27 MPA
V12
V13 V14
PM
T2

OT PD V15 R1

Wash cup V16 V17 V18 V19

V23 V24 V25 V26 UVacuumP-
V20 V21 V22 MV-Dil
Diluent
R2 Lyse
R3 MV-Lys

LVacuumP-

 P- transfers 1:200 diluted blood from PM to MC2 via SV9-7, T1, V24, V13, T2, V11, CH2
 Process stopped by BeakerD signal, around 0.5 ml remains in PM beaker 11
 Second dilution

SPa SPb

CH1

V28

M1 Dis1 Dis2 M2 V5 V6 V7 V8 V9
Shear valve Pos3 V1 V2 V3 V4

6
5 7
T1
Air pumpP+
4 8
V10 V11
BloodD
3
9
CH2

2
10 MC1 MC2
11
BeakerD
1
V27 MPA
V12
V13 V14
PM
T2

OT PD V15 R1

Wash cup V16 V17 V18 V19

V23 V24 V25 V26 UVacuumP-
V20 V21 V22 MV-Dil
Diluent
R2 Lyse
R3 MV-Lys

LVacuumP-

 P+ pushes diluent from Dis1 to MC1 via V9, CH1, V17, SV8-10, V12; lyze from Dis2 to MC2 via V21, T1,
V24, V13, T2 12
 MC1 air vent via V3; MC2 air vent via V4, V28
 Prepare PM beaker wash

SPa SPb

CH1

V28

M1 Dis1 Dis2 M2 V5 V6 V7 V8 V9
Shear valve Pos1 V1 V2 V3 V4

6
5 7
Air pumpP+
4 8
V10 V11
BloodD
3
9
CH2

2
10 MC1 MC2
11
BeakerD
1
V27 MPA
V12
V13 V14
PM

OT PD V15 R1

Wash cup V16 V17 V18 V19

V23 V24 V25 V26 UVacuumP-
V20 V21 V22 MV-Dil
Diluent
R2 Lyse
R3 MV-Lys

LVacuumP-

 P- moves 2-3 ml Dis1 via CH2, V5 CH1, V22 and MV-Dil by timing
13
 Wash PM beaker

SPa SPb

CH1

V28

M1 Dis1 Dis2 M2 V5 V6 V7 V8 V9
Shear valve Pos1 V1 V2 V3 V4

6
5 7
T1
Air pumpP+
4 8
V10 V11
BloodD
3
9
CH2

2
10 MC1 MC2
11
BeakerD
1
V27 MPA
V12
V13 V14
PM
T2

OT PD V15 R1

Wash cup V16 V17 V18 V19

V23 V24 V25 V26 UVacuumP-
V20 V21 V22 MV-Dil
Diluent
R2 Lyse
R3 MV-Lys

LVacuumP-

 P+ pushes diluent from Dis1 to PM via V9, CH1, V16, MPA

 PM air vent via V28 14
 Drain PM beaker

SPa SPb

CH1

V28

M1 Dis1 Dis2 M2 V5 V6 V7 V8 V9
Shear valve Pos1 V1 V2 V3 V4

6
5 7
T1
Air pumpP+
4 8
V10 V11
BloodD
3
9
CH2

2
10 MC1 MC2
11
BeakerD
1
V27 MPA
V12
V13 V14
PM
T2

OT PD V15 R1

Wash cup V16 V17 V18 V19

V23 V24 V25 V26 UVacuumP-
V20 V21 V22 MV-Dil
Diluent
R2 Lyse
R3 MV-Lys

LVacuumP-

 P- empties PM via CH2, V23, SV7-9

 PM ventilated by MPA, V1 and PD needle 15
 Prepare for counting

SPa SPb

CH1

V28

M1 Dis1 Dis2 M2 V5 V6 V7 V8 V9
Shear valve Pos3 V1 V2 V3 V4

6
5 7
T1
Air pumpP+
4 8
V10 V11
BloodD
3
9
CH2

2
10 MC1 MC2
11
BeakerD
1
V27 MPA
V12
V13 V14
PM
T2

OT PD V15 R1

Wash cup V16 V17 V18 V19

V23 V24 V25 V26 UVacuumP-
V20 V21 V22 MV-Dil
Diluent
R2 Lyse
R3 MV-Lys

LVacuumP-

 P+ loads SP to pressurize MC1/2 via V9, V7

16
 Count

SPa SPb

CH1

V28

M1 Dis1 Dis2 M2 V5 V6 V7 V8 V9
Shear valve Pos3 V1 V2 V3 V4

6
5 7
T1
Air pumpP+
4
BloodD 8
V10 V11
3
9
CH2

2
10 MC1 MC2
11
BeakerD
1
V27 MPA
V12
V13 V14
PM
T2

OT PD V15 R1

Wash cup V16 V17 V18 V19

V23 V24 V25 V26 UVacuumP-
V20 V21 V22 MV-Dil
Diluent
R2 Lyse
R3 MV-Lys

LVacuumP-

 P+ via Spa/b creates pressure in MC1/2, cells cross aperture, MC1 via V15, MC2 via V26, 270 ul counted
 P- fills reagent pipettes Dis1/Dis2. Dil: MV-dil, V20; Lyz: MV-Lyz 17
 Wash after count

SPa SPb

CH1

V28

M1 Dis1 Dis2 M2 V5 V6 V7 V8 V9
Shear valve Pos1 V1 V2 V3 V4

6
5 7
Air pumpP+
4
BloodD 8
V10 V11
3
9
CH2

2
10 MC1 MC2
11
BeakerD
1
V27 MPA
V12
V13 V14
PM

OT PD V15 R1

Wash cup V16 V17 V18 V19

V23 V24 V25 V26 UVacuumP-
V20 V21 V22 MV-Dil
Diluent
R2 Lyse
R3 MV-Lys

LVacuumP-

 MC1 empties through V10, CH2, P-, air vent from V3. MC2 empties through V11, CH2, P-, air vent from
V4, V28. Vent line for MC1/2 are closed for a short time and vacuum are created. MC1 fills up with diluent 18
through V19, V12. MC2 fills up with diluent through V25, V13
 Ready for next analysis

SPa SPb

CH1

V28

M1 Dis1 Dis2 M2 V5 V6 V7 V8 V9
Shear valve Pos1 V1 V2 V3 V4

6
5 7
Air pumpP+
4 8
V10 V11
BloodD
3
9
CH2

2
10 MC1 MC2
11
BeakerD
1
V27 MPA
V12
V13 V14
PM

OT PD V15 R1

Wash cup V16 V17 V18 V19

V23 V24 V25 V26 UVacuumP-
V20 V21 V22 MV-Dil
Diluent
R2 Lyse
R3 MV-Lys

LVacuumP-

 After a cycle, instrument returns to initial state, ready for next analysis
 4 pipettes full, 2 measuring chamber filled 19
 PD sample aspiration (prediluted blood)

SPa SPb

CH1

V28

M1 Dis1 Dis2 M2 V5 V6 V7 V8 V9
Shear valve Pos1 V1 V2 V3 V4

6
5 7
Air pumpP+
4 8
V10 V11
BloodD
3
9
CH2

2
10 MC1 MC2
11
BeakerD
1
V27 MPA
V12
V13 V14
PM

OT PD V15 R1

Wash cup V16 V17 V18 V19

V23 V24 V25 V26 UVacuumP-
V20 V21 V22 MV-Dil
Diluent
R2 Lyse
R3 MV-Lys

LVacuumP-

 P- creates vacuum in PM by loading SP via CH2, V5, CH1, V7, SP, V4

 PD blood sample directly aspirated in PM via V1, MPA, 20
 Proceeds to “Prepare for second dilution”
 Run micro capillary sample (MPA)
SPa SPb

CH1

V28

M1 Dis1 Dis2 M2 V5 V6 V7 V8 V9
Shear valve Pos1 V1 V2 V3 V4

6
5 7
T1
Air pumpP+
4 8
V10 V11
BloodD
3
9
CH2

2
10 MC1 MC2
11
BeakerD
1
V27 MPA
V12
V13 V14
PM
T2

OT PD V15 R1

Wash cup V16 V17 V18 V19

V23 V24 V25 V26 UVacuumP-
V20 V21 V22 MV-Dil
Diluent
R2 Lyse
R3 MV-Lys

LVacuumP-

 P+ pushes diluent from Dis1 to PM via V9, CH1, V16, MPA, brings blood sample in MPA to PM
 Proceeds to “prepare to second dilution”. 21