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Collection and Handling of Specimen For Microbial Examination

1. Proper collection and handling of clinical specimens for microbial examination is important. Specimens should be collected before antimicrobial agents, on the correct stage of disease and site of infection, with minimal contamination and in a sterile manner. 2. Common specimens include blood, CSF, urine, sputum, feces, and mucous membrane secretions. Each has specific collection and handling methods to optimize microbial recovery and identification. 3. It is important to provide sufficient specimen quantity, promptly deliver samples to the laboratory, and include relevant clinical information to aid in microbial analysis.

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Franz Earl Niño Albesa
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146 views

Collection and Handling of Specimen For Microbial Examination

1. Proper collection and handling of clinical specimens for microbial examination is important. Specimens should be collected before antimicrobial agents, on the correct stage of disease and site of infection, with minimal contamination and in a sterile manner. 2. Common specimens include blood, CSF, urine, sputum, feces, and mucous membrane secretions. Each has specific collection and handling methods to optimize microbial recovery and identification. 3. It is important to provide sufficient specimen quantity, promptly deliver samples to the laboratory, and include relevant clinical information to aid in microbial analysis.

Uploaded by

Franz Earl Niño Albesa
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PPTX, PDF, TXT or read online on Scribd
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Collection and Handling of Specimen

for Microbial Examination


Considerations in collecting samples
1. Collection must be done before any
antimicrobial agent was introduced into the patient
2. Collect the specimen on the right stage of the
disease
3. Collect on the proper site of infection
4. Collect the specimen with little contamination
as posible
5. Must be collected in sterile manner
6. Collect sufficient quantity of the specimen
7. Prompt delivery to the laboratory
8. Laboratory must be given sufficient clinical
information
Clinical/Biological Specimens
1. Blood
- collected through venipuncture
- anticoagulant of choice: SPS (Sodium
Polyanethol sulfonate)
10 – 12 ml
sample

5 ml to 50 ml 5 ml to
TSB Thioglycolate Other media
(1:10 ratio) (anaerobic culture) (BHI)
2. CSF
- collected through lumbar puncture
- collected in 3 sterile tubes/vials
tube 1 - last to be filled, discarded
tube 2 – for bacterial analysis
tube 3 - last to be filled, for cell count
and
Supernate chemistry
CSF analysis
Sediments

Serological Chemistry Gramstainin


Culture
analysis analysis g
3. Urine
- collection methods
- Mid-stream catch
- Suprapubic aspiration
- Catheterization
MHA (colony
count)
MSA (Gram-positive
cocci)
Urine sample EMB/MAC (Gram –
negative

BAP (Fastidios m.o)


4.

- Direct smear - Grams and AFB staining


- Concentration technique
- Culture (Petragnani, MTM)
5. Feces/Stool

- Screening: Gram-staining
- Culture : Differential media (SSA, XLD,
EMB)
Enrichment media (GN, selinite F)
- Biochemical testing
6. Mucous Membrane Secretion
- collected by using cotton/polyester swab
a. Transudates/Exudates
1. Peritoneal fluid
2. Synovial fluid
3. Pleural fluid
b. Secretions from the following
1. Wound
2. Throat
3. Ear
4. Nose
5. Rectum
6. Operative sites
7. Ulceration
8. Eyes
9. Urogenital openings

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