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Applications of High Performance Liquid Chromatography (HPLC) in To Inorganic and Organometallic Compounds

This document provides an overview of high performance liquid chromatography (HPLC) and its applications. It discusses the key components of HPLC systems including the pump, injector, column, detector, and computer. The pump forces the mobile phase through the system at high pressures. The injector introduces samples into the mobile phase flow. The column separates sample components using interactions with its stationary phase. The detector measures components as they elute from the column. The computer collects output from the detector to analyze retention times and component amounts. Objectives include understanding HPLC analysis and component functions, and applying HPLC to inorganic and organometallic compounds.

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Tuba Ahmed
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270 views16 pages

Applications of High Performance Liquid Chromatography (HPLC) in To Inorganic and Organometallic Compounds

This document provides an overview of high performance liquid chromatography (HPLC) and its applications. It discusses the key components of HPLC systems including the pump, injector, column, detector, and computer. The pump forces the mobile phase through the system at high pressures. The injector introduces samples into the mobile phase flow. The column separates sample components using interactions with its stationary phase. The detector measures components as they elute from the column. The computer collects output from the detector to analyze retention times and component amounts. Objectives include understanding HPLC analysis and component functions, and applying HPLC to inorganic and organometallic compounds.

Uploaded by

Tuba Ahmed
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd
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Applications of High performance

liquid chromatography (HPLC) in


to inorganic and organometallic
compounds
CHM-4191/631
Course Title: Spectroscopy and chromatography
Course Incharge: Dr. Ishrat Jamil
Lecture content
 Introduction to HPLC
 Components of HPLC
 Working of the components of HPLC
 Block diagram of HPLC with the explaination

of the separation process


 Applications of HPLC
Objectives/learning outcomes
 At the end of this unit, you should be able to-
 Identify HPLC analysis
 Describe the function of the various

components of a HPLC
 Application of HPLC for inorganic and organo

metallic compounds
Introduction to High performance
liquid chromatography (HPLC)
Components of HPLC
Pump
 The role of the pump is to force a mobile
phase through the liquid chromatograph at a
specific flow rate, expressed in mL /min
 Normal flow rates in HPLC are in the 1-to 2-

mL/min range.
 Typical pumps can reach pressures in the

range of 6000-9000 psi (400-to 600-bar).


Injector
 The injector serves to introduce the liquid
sample into the flow stream of the mobile
phase.
 Typical sample volumes are 5-to 20-

microliters (μL).
 The injector must also be able to withstand

the high pressures of the liquid system.


 An auto sampler is the automatic version for

when the user has many samples to analyze


or when manual injection is not practical
column
 Considered the “heart of the chromatograph”
the column’s stationary phase separates the
sample components of interest using various
physical and chemical parameters.
 The small particles inside the column are what

cause the high back pressure at normal flow


rates.
 The pump must push hard to move the mobile

phase through the column and this resistance


causes a high pressure within the
chromatograph.
Detector
 The detector can detect the individual
molecules that come out from the column.
 A detector serves to measure the amount of

those molecules so that the chemist can


quantitatively analyze the sample
components.
 The detector provides an output to a recorder

or computer that results in the liquid


chromatogram(i.e., the graph of the detector
response).
Computer
 Frequently called the data system, The
computer takes the signal from the detector
and uses it to
 determine the time of elution (retention time)

of the sample components (qualitative


analysis) and
 the amount of sample ( quantitative analysis)

.
Block diagram for HPLC
Short summary of the components
Cont..
APPLICATIONS
References and further reading

 Skoog, Holler, and Neiman. Principles of Instrumental


Analysis. 5 th ed. Orlando: Harcourt Brace & Co., 1998.
 Handbook of HPLC [electronic resource] / edited by Katz,
New York: M. Dekker, 1998.

 http://192.215.107.101/ebn/942/tech/techfoc
us/1071main.html
 Introduction to Modern Liquid Chromatography,
Lloyd R. Snyder, Joseph J. Kirkland, and John W. Dolan, 3rd
Ed Wiley; 3 edition ,December 9, 2009.
 Interactive secession will be conducted from 10.00am to

3.00pm on Lms discussion Forum and 24/7 on whatsapp.

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