GENERAL BIOLOGY 2

SPECIALIZED SUBJECT|ACADEMIC STEM

 OUTLINE

Introduction Chapter 3: Systematics Based on Evolutionary

Relationships
DepEd General Biology 2 Curriculum Guide
• Lesson 14: Systematics Based on Evolutionary Relationships:
Tree of Life and Systematics
Chapter 1: Genetics • Lesson 15: Systematics Based on Evolutionary Relationships:
• Lesson 1: Pedigree Analysis Taxonomy
• Lesson 2: Sex Linkage and Recombination • Lesson 16: Systematics Based on Evolutionary Relationships:
• Lesson 3: Modifications to Mendel’s Classic Ratios Cladistics and Phylogeny
• Lesson 4: Molecular Structure of DNA, RNA, and Proteins Chapter 4: Compare and Contrast Processes in
• Lesson 5: DNA Replication and Protein Synthesis
Plants and Animals
• Lesson 6: Genetic Engineering
• Lesson 7: Discuss the Applications of Recombinant DNA • Lesson 17: Reproduction and Development
• Lesson 18: Nutrition
Chapter 2: Evolution and Origin of Biodiversity • Lesson 19: Gas Exchange
• Lesson 8: History of Life on Earth • Lesson 20: Transport and Circulation
• Lesson 9: Mechanisms that Produce Change in Populations • Lesson 21: Regulation of Body Fluids
• Lesson 10: Evolution and Origin of Biodiversity: Patterns of • Lesson 22: Immune Systems
Descent with Modification • Lesson 23: Chemical and Nervous Control
• Lesson 11: Development of Evolutionary Thought • Lesson 24: Sensory and Motor Mechanisms
• Lesson 12: Evidences of Evolution • Lesson 25: Feedback Mechanisms
• Lesson 13: Infer Evolutionary Relationships of Organisms
 INTRODUCTION: SHS FOR SHS FRAMEWORK

Through this Teaching Guide, Given that developing mastery When teachers empower
teachers will be able to facilitate goes beyond memorization, learners to take ownership of
an understanding of the value teachers should also aim for their learning, they develop
of the lessons, for each learner deep understanding of the independence and self direction,
to fully engage in the content subject matter where they lead learning about both
on both the cognitive and learners to analyze and the subject matter and
affective levels. synthesize knowledge. themselves.
DepEd General Biology 2 Curriculum Guide
Chapter 1: Genetics
Lesson 1: Pedigree Analysis
RELEVANT VOCABULARY

Pedigree
• Making use of diagrams showing the ancestral relationships and transmission of genetic traits over
several generations in a family

Proband
• The individual in the pedigree that led to the construction of the pedigree.
• For example, a couple consults a medical geneticist because they have an offspring who is afflicted
with a disease and they want to find out the mode of transmission of this disease.
• When the medical geneticist constructs the pedigree, the offspring will be labeled as the proband.

Through the pedigree, the probability of having other affected children may be
determined.
Pedigree analysis

With diagrams showing the

ancestral relationships and
transmission of genetic traits
over several generations

E.g. humans, dogs, cattle

Autosomal Recessive Trait

Either I-3 or I-4 must

be heterozygous

Recessive traits
typically skip
generations

Recessive autosomal
traits appear equally in
both sexes
Autosomal Dominant Trait

I-1 heterozygous for a

dominant allele

Dominant traits almost

always appear in each
generation

Affected individuals all have

affected parent.

Dominant autosomal traits

appear equally in both sexes
Genetic Terminology

• any characteristic that can be passed from

Trait parent to offspring

Heredity • passing of traits from parent to offspring

Autosomal • A trait whose alleles that control it are

found in the autosomes (body
trait chromosomes/ non-sex chromosomes)
Types of Genetic Crosses

Monohybrid cross
• cross involving a single trait
e.g. flower color
Dihybrid cross
• cross involving two traits
e.g. flower color & plant height
 • two forms of a gene (dominant &
Alleles recessive)

• stronger of two genes expressed

Dominant in the hybrid; represented by a
capital letter (R)

• gene that shows up less often in

Recessive a cross; represented by a
lowercase letter (r)
GENOTYPE

gene combination for a trait (e.g. RR, Rr, rr)

the gene pair an individual carries for a particular trait

symbolized with a pair of letters.

By convention, uppercase letter (eg. A) for a dominant allele

and lowercase letter (eg. a) for the recessive allele.

Any letter in the alphabet may be used.

GENOTYPE

For a diploid organism with two alleles in a given gene pair,

genotypes may be written as:

i. Homozygous dominant, i.e. with two dominant alleles (DD)

ii. Heterozygous, i.e. with a dominant and recessive allele (Dd).

The individual will show the dominant phenotype.

iii. Homozygous recessive, i.e. with two recessive alleles (dd)

PHENOTYPE

The observable trait of an individual based

on its genotype.

Examples: red flower, curly hair, blood

types ( i.e. the blood type is the
phenotype)
 PHENOTYPE

For a typical Mendelian trait, phenotypes may either be:

i. Dominant.
• A trait that requires at least one dominant allele for the trait to be
expressed, e.g. Dd
ii. Recessive.
• A trait that requires two recessive alleles for the trait to be expressed
 Genotype & Phenotype in Flowers

Genotype of alleles:
R = red flower
r = yellow flower
All genes occur in pairs, so 2
alleles affect a characteristic
Possible combinations are:
Genotypes RR Rr rr
Phenotypes RED RED YELLOW
 Punnett Square

Used to help
solve
genetics
problems
MONOHYBRID CROSSES

27
 P 1 MONOHYBRID CROSS
Trait: Seed Shape
Alleles: R – Round r – Wrinkled
Cross: Round seeds x Wrinkled seeds
RR x rr
Genotype: Rr
r r
Phenotype: Round

R Rr Rr Genotypic
Ratio: All alike

R Rr Rr Phenotypic
Ratio: All alike
28
 P 1 MONOHYBRID CROSS REVIEW

Homozygous dominant x Homozygous recessive

Offspring all Heterozygous (hybrids)

Offspring called F1 generation

Genotypic & Phenotypic ratio is ALL ALIKE

29
 F 1 MONOHYBRID CROSS
Trait: Seed Shape
Alleles: R – Round r – Wrinkled
Cross: Round seeds x Round seeds
Rr x Rr
Genotype: RR, Rr, rr
R r
Phenotype: Round &
wrinkled
R RR Rr
G.Ratio: 1:2:1

r Rr rr P.Ratio: 3:1

30
 F 1 MONOHYBRID CROSS REVIEW

Heterozygous x heterozygous
• Offspring:
25% Homozygous dominant RR
50% Heterozygous Rr
25% Homozygous Recessive rr

Offspring called F2 generation

Genotypic ratio is 1:2:1

Phenotypic Ratio is 3:1

31
WHAT DO THE PEAS LOOK LIKE?

32
 …AND NOW THE TEST CROSS

Mendel then crossed a pure & a hybrid from his F2 generation

This is known as an F2 or test cross

There are two possible testcrosses:

• Homozygous dominant x Hybrid
• Homozygous recessive x Hybrid

33
 F 2 MONOHYBRID CROSS (1 ST )
Trait: Seed Shape
Alleles: R – Round r – Wrinkled
Cross: Round seeds x Round seeds
RR x Rr
Genotype: RR, Rr
R r
Phenotype: Round

R RR Rr Genotypic
Ratio: 1:1

R RR Rr Phenotypic
Ratio: All alike
34
 F 2 MONOHYBRID CROSS (2ND)
Trait: Seed Shape
Alleles: R – Round r – Wrinkled
Cross: Wrinkled seeds x Round seeds
rr x Rr

R r Genotype: Rr, rr
Phenotype: Round &
r Rr rr Wrinkled
G. Ratio: 1:1
r Rr rr P.Ratio: 1:1
35
 F 2 MONOHYBRID CROSS REVIEW

Homozygous x heterozygous(hybrid)

• Offspring:
50% Homozygous RR or rr
50% Heterozygous Rr

Phenotypic Ratio is 1:1

Called Test Cross because the offspring have SAME genotype as

parents
36
MENDEL’S LAWS

37
RESULTS OF MONOHYBRID
CROSSES

Inheritable factors or genes are responsible for all heritable characteristics

Phenotype is based on Genotype

Each trait is based on two genes, one from the mother and the other from the
father

True-breeding individuals are homozygous ( both alleles) are the same

38
LAW OF DOMINANCE
In a cross of parents that are pure for contrasting traits, only one form of the trait will appear in the next
generation.

All the offspring will be heterozygous and express only the dominant trait.

RR x rr yields all Rr (round seeds)

39
LAW OF DOMINANCE

40
LAW OF SEGREGATION

During the formation of gametes (eggs or sperm), the two alleles responsible for a trait separate
from each other.

Alleles for a trait are then "recombined" at fertilization, producing the genotype for the traits of
the offspring.

41
APPLYING THE LAW OF SEGREGATION

42
LAW OF INDEPENDENT ASSORTMENT

Alleles for different traits are distributed to sex cells

(& offspring) independently of one another.

This law can be illustrated using dihybrid crosses.

43
DIHYBRID CROSS
A breeding experiment that tracks the inheritance of two traits.

Mendel’s “Law of Independent Assortment”

a. Each pair of alleles segregates independently during gamete formation

b. Formula: 2n (n = # of heterozygotes)
44
 QUESTION:
HOW M ANY GAMETES WILL BE P RODUCED FOR THE FOLLOWING ALLELE ARRANGEMENTS?

Remember: 2n (n = # of heterozygotes)

1. RrYy

2. AaBbCCDd

3. MmNnOoPPQQRrssTtQq
45
 ANSWER:
1. RrYy: 2n = 22 = 4 gametes
RY Ry rY ry

2. AaBbCCDd: 2n = 23 = 8 gametes
ABCD ABCd AbCD AbCd
aBCD aBCd abCD abCD

3. MmNnOoPPQQRrssTtQq: 2n = 26 = 64
gametes
46
 DIHYBRID CROSS
Traits: Seed shape & Seed color
Alleles: R round
r wrinkled
Y yellow
y green

RrYy x RrYy

RY Ry rY ry RY Ry rY ry

All possible gamete combinations

47
DIHYBRID CROSS
RY Ry rY ry

RY

Ry

rY

ry

48
 DIHYBRID CROSS

RY Ry rY ry

RY RRYY Round/Yellow: 9
RRYy RrYY RrYy

Round/green: 3
Ry RRYy RRyy RrYy Rryy

wrinkled/Yellow: 3
rY RrYY RrYy rrYY rrYy
wrinkled/green: 1
ry RrYy Rryy rrYy rryy 9:3:3:1 phenotypic
ratio
49
DIHYBRID CROSS

Round/Yellow: 9
Round/green: 3
wrinkled/Yellow: 3
wrinkled/green: 1
9:3:3:1

50
OTHER TERMS

●
A trait that is expressed due to
Phenoco ●
specific environmental conditions
(i.e. having hair that is dyed of a
py different color) and is not due to
the genotype
OTHER TERMS

Identical ●

●
monozygotic twins; derived from a single fertilization event
After the first cleavage or cell division of the zygote, the
cells or blastomeres separate and become independent

twins blastocysts implanted in the mother’s uterus

Fraternal ●
derived from separate fertilization events (two
eggs fertilized by two sperms) within the fallopian
tube, resulting in two separate zygotes
twins ●
dizygotic twins
TWIN STUDY

Determines which plays a

more significant role in the
expression of a trait:
heredity or environment

Uses identical twins that

are separated from birth
Lesson 2: SEX LINKAGE AND RECOMBINATION
Sex Determination & Sex-Linked Traits

Red-green colourblindness

Hemophilia

Duchenne muscular dystrophy

What is the difference between an Autosome and
a Sex-chromosome?
 Autosomes are the first
22 homologous pairs of
human chromosomes
that do not influence
the sex of an individual.

 Sex Chromosomes are

the 23rd pair of
chromosomes that
determine the sex of an
individual
Sex Determination:

How many chromosomes do humans have?

How many of these are “sex” chromosomes?

Which parent determines the sex of an offspring?

Are there any traits/conditions that are more common

in one sex than the other?
Sex Chromosomes:

Which is male?… female?

What is the genotype of each sex?
Which parent determines the sex of the offspring?
 Sex • The gene (pair) that determines a
linked character (e.g. hemophilia) is
located on the sex chromosomes
trait
X-linked • A sex-linked trait is where the
gene or allele for the trait is found
trait on the X chromosome

Y-linked • A sex-linked trait where the gene

or allele for the trait is found on
trait the Y- chromosome
 Color blindness
 An X-linked recessive trait where a affected individual could
not distinguish red from green color (red green color
blindness)
 Hemophilia
 An X-linked recessive trait where an affected individual
suffers from delayed blood clotting during injuries because
of the absence of certain blood clotting factors
 Hypertrichosis pinnae auris
 A Y-linked trait where affected males have hair growing
from their external ears
 Can you see the hidden numbers?
Colourblindness Self Test:

NUMBERS: 5 | 8 | 9 | 5 | 3 | 5 | 9 | 10 |
Hidden Shapes:

SHAPES:
Plate 1 - Circle and arch
Plate 2 - Circle, star and square
Sex-Linked Traits:
•Some genes are found on the sex chromosomes
•Some are found on the X sex chromosome but not the Y
Ex – the colour vision gene is on the X chromosome
- NOT FOUND ON THE “Y”

Sex-linked traits are RECESSIVE (mostly) traits

that are found on the ‘x’ sex chromosome
 Hypertrichosis –
Human Werewolf Syndrome:
Congenital generalized
hypertrichosis (CGH)
Rare, X-linked dominant trait
Found in a single multigenerational
Mexican family
Red-green colorblindness is a recessive sex-linked trait, found on the X chromosome, not the Y.

Males only have one X chromosome, they have a much greater chance of having red-green colorblindness.

Females would have to be homozygous recessive in order to have red-green colorblindness.

Writing Sex-linked genotypes:
More practice:
 In humans the gene from normal blood clotting, H, is
dominate to the gene for hemophilia, h. This is a
sex-linked trait found on the X chromosome. Serena
with normal blood clotting has four children. They
are a normal son, a hemophiliac son, and two
normal daughters. The father, Simon has normal
blood clotting. What is the probable genotype for
each member of the family?
Another one….
John Lloyd with normal vision marries
Bea who is a carrier for color
blindness. What are the possible
genotypes and phenotypes of their
children.
Just one more….
A hemophiliac man, Rudy marries
Lenny who is a carrier for the disease.
What are the possible genotypes and
phenotypes of their children? Should
they even consider having children?
OTHER SEX-RELATED TRAITS
Sex influenced characteristics
• are determined by autosomal
genes and are inherited
according to Mendel’s principles,
but they are expressed differently
in males and females
Pattern baldness is a sex-influenced trait

This trait is seen in three generations of the Adams family: a) John Adams, 2nd President of
USA, was father to b) John Quincy Adams, who was father to c) Charles Francis Adams

Pattern baldness reults from an autosomal gene that is thought to be dominant in males and
recessive in females
Sex- Limited Characteristics
• extreme form of sex-influenced
inheritance, a sex limited characteristic
is encoded by autosomal genes that
are expressed in only one sex—the trait
has zero penetrance in the other sex
Example: Cock feathering in chickens, an autosomal recessive trait that
is limited to males

a) Cock-feathered male; b) and c) Hen-feathered females

Lesson 3: MODIFICATIONS TO MENDEL’S CLASSIC RATIOS
NON-MENDELIAN
GENETICS
WHAT COLOR OFFSPRING WOULD YOU GET
WHEN YOU CROSS A PURE (HOMOZYGOUS)
RED SNAPDRAGON WITH A PURE WHITE
SNAPDRAGON?

?
ODDLY ENOUGH, NEITHER RED NOR
WHITE
Snapdragon Flower
Color is controlled by
Incomplete
Dominance and a new
3rd phenotype is seen.
HOW DOES INCOMPLETE DOMINANCE
WORK?
INCOMPLETE DOMINANCE IS A
BLENDING
Like Paint, the
RED Pigment
“MIXES” with the
WHITE to create
PINK-
FLOWERED
offspring
INCOMPLETE DOMINANCE CAN BE
SEEN IN:
Horses ( Chestnut x Cremello 
Palomino)

Snapdragons

Japanese Four O’ Clocks

Many flowers exhibit incomplete

dominance
ALLELE NOTATION
HOW DO YOU WRITE OUT THE
GENOTYPE WHEN BOTH
ALLELES ARE DOMINANT?
YOU MUST USE DIFFERENT
LETTERS

Choose different letters to represent each form (In this case we’ll use “W” for the white allele and “R”
for the Red allele).

Remember to always use CAPITAL letters. This is incomplete DOMINANCE.

SO…THE CROSS BETWEEN PURE
SNAPDRAGONS LOOKS LIKE THIS
R R

W RW RW

W RW RW
YOU’VE SEEN A CROSS BETWEEN PURE
SNAPDRAGONS, NOW DO THE PUNNET SQUARE FOR A
CROSS BETWEEN TWO HYBRIDS (HETEROZYGOTES).

What are the alleles of a heterozygous snapdragon?

●
RW

What is the genotypic ratio?

●
1 RR : 2 RW : 1 WW

What is the phenotypic ratio?

●
1 Red : 2 Pink : 1 White
WHAT KIND OF GENETICS DO
THESE ORGANISMS EXHIBIT?
 A cross between 2 tabbies (the
CODOMINANCE heterozygotes) results in
1 black : 2 tabbies : 1 tan cat

The same ratios as Incomplete

Dominance occur:
A ratio of 1:2:1 for both
genotype and phenotypes of a
monohybrid cross


Different Phenotype:
The two original phenotypes
are combined to give a
SPOTTED or
MULTICOLORED
phenotype.
NOTATION FOR CODOMINANCE

1. Choose a letter to represent the gene.

2. Choose different letters to represent each of the
alleles.
3. Take the letters representing the alleles and turn
them into superscripts.
4. Combine the two. Remember to use capital letters.
WRITING OUT THE
GENOTYPE
1. We’re going to use C to represent Coat Color of a shorthorn cow.
2. Roan coat color is a combination of Red and White hair so we’ll
use “R” to represent Red and “W” to represent white.
3. R  R and W  W
4. C + R  CR and C + W  CW
5. So a Red Cow would be CRCR , a white cow would be CWCW, and a
roan cow would be CRCW
CODOMINANCE OCCURS IN:

Shorthorn Cow (White + Red)

Blue Roosters (White + Black)

Human Blood Typing (AB)

Tabby Cats (Black and Tan Fur)

LET’S PRACTICE
Tabby cats exhibit codominance between tan and black fur.
1. Can Tan Cat be heterozygous? Why or Why not?
2. If you mate a tabby with a black cat, what is the phenotypic ratio
of their offspring?
3. If the offspring of two cats are composed of 36 tabbies and 40
black cats, what are the parental phenotypes and genotypes?
BLOOD TYPING

Human blood typing is an example of both

CODOMINANCE and MULTIPLE ALLELES

What does Multiple Alleles Mean?

THE ALLELES

A and B blood types are coded for by the alleles:

IA and IB respectively. These two alleles are CODOMINANT.

Blood type O is coded for by the allele i and is recessive to both I A and IB (notice the lower case
letter).
ALLELES AND THEIR BLOOD
TYPES
LET’S PRACTICE
Could a man with AB blood have an AB child with a woman with
type O blood?
If a child has type B blood, what are all the possible blood types
for his/her parents?
A paternity test is being performed. The child has type A blood.
The mother is type A. Potential Dad #1 has type B blood. Potential
Dad #2 has type O blood. Who is the father?
Challenge: What two parents could produce the most diverse
children as far as blood type is concerned?
MULTIPLE ALLELES:
DUCK FEATHER PATTERNS
Three alleles determine the type of plumage in mallard
ducks:

MR (Restricted) > M (Mallard) > md (Dusky)

 MULTIPLE ALLELES:
COAT COLOUR IN RABBITS
Four phenotypes and four alleles:

Allelic series is C > cch > ch > c (which is most dominant)

Coat Color Phenotype Genotype

Full Color CC, Ccch, Cch, Cc
Chinchilla cchcch, cchch, cchc
Himalayan chch, chc
Albino cc
Lesson 4: MOLECULAR STRUCTURE OF DNA,RNA, AND PROTEINS
• What is the function of DNA, RNA & PROTEIN?

DNA • repository of genetic information; sequence of bases

encodes the blueprint for life processes

• information in the form of base sequence is

RNA transformed (transcribed) into mRNA, tRNA and rRNA.

DNA is the template copied into RNA by base pairing.
G with C; A with U.

Protein • functional products of genes; executes cellular

functions
• What is the center of heredity?

 Eukaryotic organisms
 Nucleus

 Prokaryotic organisms
 Nucleoid region

 Viruses
 Protein coat (viral head or capsid)
 • What is the genetic material?

 DNA serves as the molecule storing genetic

information

 DNA or RNA in viruses

 • What do DNA and RNA stand for?

DNA – deoxyribonucleic acid

RNA – ribonucleic acid

Types of nucleic acids

1. Deoxyribonucleic acid (DNA)

• With pentose DEOXYRIBOSE sugar backbone
2. Ribonucleic acid (RNA)
• With pentose RIBOSE sugar backbone
 • How is DNA organized to serve as the
genetic material?

 single stranded in a few viruses

 molecule organized as a double helixdouble-
stranded

 Contained within each DNA molecule are hereditary

units called genes, which are part of larger
elements, the chromosomes
DNA AS THE GENETIC MATERIAL
Packaging of DNA in a eukaryotic chromosome
DNA AS THE GENETIC MATERIAL
Packaging of DNA in a eukaryotic chromosome
DNA AS THE GENETIC MATERIAL
Packaging of DNA in a eukaryotic chromosome

Nucleosome
•DNA+8 histone molecules
 2x(H2A, H2B, H3 & H4)
CHARACTERISTICS OF A GENETIC MATERIAL

Stable • does not easily denature

Replicable • sequence can be copied

• sequence can be converted into a

Translatable different language

Mutable • can be changed to yield variations

 • How does the DNA store genetic
information?

 Nucleotides
 Four different forms of chemical building blocks
in a segment of the DNA constituting a gene

 sequencemaking up a gene encodes the

chemical nature of a protein
Nucleic acids are made up of sequences of NUCLEOTIDES

Components of nucleotides:

1. Pentose sugar backbone

2. Phosphate group

3. Nitrogen base
 • How is the genetic code organized?

4 different nucleotides in DNA, varying in one

of its components, the nitrogenous base

 geneticcode is triplet: combination of 3

nucleotides
 All possible codes specify one of 20 amino acids
Types of nitrogenous bases
• How is the genetic code expressed?

Transcription

Messenger RNA
(mRNA)

Ribosome

Translation

Protein
 • Are there exceptions where proteins are
not the end product of a gene?

 Genescoding for ribosomal RNA (rRNA),

which is part of the ribosome

 Transfer RNA (tRNA), which is involved in the

translation process, are transcribed but not
translated

 RNAis sometimes the end product of stored

genetic information
 • Why are proteins so important to living
organisms that they serve as the end product of
the vast majority of genes?

 Serve
as highly specific biological catalysts, or
enzymes

 Perform non-enzymatic roles

What is the complementary strand
of the DNA molecule 5'
AACGGGTTTATGCGT 3'?
A. 5' TTGCCCAAATACGCA 3‘
B. 3' AACGGGTTTATGCGT 5'
C. 5' ACGCATAAACCCGTT 3'
D. 3' TTGCCCAAATACGCA 5'
E. Both C and D are complementary strands.
What is the complementary strand of
the following RNA strand:
5' AUGCGUUUACCGA 3'?

A. 3' AGCCAUUUGCGUA 5'

B. 3' UACGCAAUGGCU 5'
C. 3' TACGCAAATGGCT 5
D. 3' AUGCGUUUACCGA 3'
E. none of the above.
Lesson 5: DNA REPLICATION AND PROTEIN SYNTHESIS
DNA Replication

Models of DNA replication: Meselson-Stahl Experiment

DNA synthesis and elongation

DNA polymerases

Origin and initiation of DNA replication

Prokaryote/eukaryote models (circular/linear

chromosomes)

Telomere replication
ALTERNATIVE MODELS OF DNA REPLICATION
1955: Arthur Kornberg

Worked with E. coli.

Discovered the mechanisms of DNA synthesis.

Four components are required:

1. dNTPs: dATP, dTTP, dGTP, dCTP

(deoxyribonucleoside 5’-triphosphates)
(sugar-base + 3 phosphates)

2. DNA template

3. DNA polymerase (Kornberg enzyme)

4. Mg 2+
(optimizes DNA polymerase activity)

1959: Arthur Kornberg (Stanford University) & Severo Ochoa (NYU)

Three main features of the DNA synthesis reaction:

1. DNA polymerase I catalyzes formation of phosphodiester

bond
between 3’-OH of the deoxyribose (on the last
nucleotide) and
the 5’-phosphate of the dNTP.

• Energy for this reaction is derived from the release of

two of the three phosphates of the dNTP.

2. DNA polymerase “finds” the correct complementary

dNTP at each step in the lengthening process.

• rate ≤ 800 dNTPs/second

• low error rate

3. Direction of synthesis is 5’ to 3’
DNA polymerase
Image credit:
Protein Data Bank
DNA ELONGATION
DNA ELONGATION
There are many different types of DNA polymerase

Polymerase Polymerization (5’-3’) Exonuclease (3’-5’) Exonuclease (5’-3’) #Copies

I Yes Yes Yes 400

II Yes Yes No ?

III Yes Yes No 10-20

3’ to 5’ exonuclease activity = ability to remove nucleotides from the

3’ end of the chain

 Important proofreading ability

 Without proofreading error rate (mutation rate) is 1 x 10 -6

 With proofreading error rate is 1 x 10 -9 (1000-fold decrease)

5’ to 3’ exonuclease activity functions in DNA replication & repair.

Eukaryotic enzymes:

Five common DNA polymerases from mammals.

1. Polymerase  (alpha): nuclear, DNA replication, no proofreading

2. Polymerase  (beta): nuclear, DNA repair, no proofreading

3. Polymerase  (gamma): mitochondria, DNA repl., proofreading

4. Polymerase  (delta): nuclear, DNA replication, proofreading

5. Polymerase  (epsilon): nuclear, DNA repair (?), proofreading

• Different polymerases for the nucleus and mtDNA

• Some polymerases proofread; others do not.

• Some polymerases used for replication; others for repair.

• Polymerases vary by species.

Origin of replication (e.g., the prokaryote example):

 Begins with double-helix denaturing into single-strands thus exposing the

bases.

 Exposes a replication bubble from which replication proceeds in both

directions.

~245 bp in E. coli
Initiation of replication, major elements:

 Segments of single-stranded DNA are called template strands.

 Gyrase (a type of topoisomerase) relaxes the supercoiled DNA.

 Initiator proteins and DNA helicase binds to the DNA at the replication
fork and untwist the DNA using energy derived from ATP (adenosine
triphosphate).
(Hydrolysis of ATP causes a shape change in DNA helicase)

 DNA primase next binds to helicase producing a complex called a

primosome (primase is required for synthesis),

 Primase synthesizes a short RNA primer of 10-12 nucleotides, to which

DNA polymerase III adds nucleotides.

 Polymerase III adds nucleotides 5’ to 3’ on both strands beginning at the

RNA primer.

 The RNA primer is removed and replaced with DNA by polymerase I, and
the gap is sealed with DNA ligase.

 Single-stranded DNA-binding (SSB) proteins (>200) stabilize the single-

stranded template DNA during the process.
Model of replication in E. coli
DNA replication is continuous on the leading strand and semidiscontinuous
on the lagging strand:

Unwinding of any single DNA replication fork proceeds in one direction.

The two DNA strands are of opposite polarity, and DNA polymerases only
synthesize DNA 5’ to 3’.

Solution: DNA is made in opposite directions on each template.

• Leading strand synthesized 5’ to 3’ in the direction of the

replication fork movement.

continuous

requires a single RNA primer

• Lagging strand synthesized 5’ to 3’ in the opposite

direction.

semidiscontinuous (i.e., not continuous)

requires many RNA primers , DNA is

synthesized in short fragments.
Supercoiled DNA relaxed by gyrase & unwound by helicase + proteins:

5’ SSB Proteins
Okazaki Fragments
1 ATP

Polymerase III 2
Helicase
Lagging strand 3 +
Initiator Proteins

3’
primase base pairs

Polymerase III 5’

RNA primer replaced by polymerase I

& gap is sealed by ligase
3’
’ 
5
Leading strand
RNA Primer

3’
Model of DNA Replication
DNA ligase seals the gaps between
Okazaki fragments with a
phosphodiester bond
Model of DNA replication

Peter J. Russell, iGenetics: Copyright © Pearson Education, Inc., publishing as Benjamin Cummings.
Model of DNA replication

Peter J. Russell, iGenetics: Copyright © Pearson Education, Inc., publishing as Benjamin Cummings.
DNA replication in eukaryotes:

Copying each eukaryotic chromosome during the S phase

of the cell cycle presents some challenges:

Major checkpoints in the system

1. Cells must be large enough, and the environment

favorable.

2. Cell will not enter the mitotic phase unless all the DNA
has replicated.

3. Chromosomes also must be attached to the mitotic

spindle for mitosis to complete.

4. Checkpoints in the system include proteins call cyclins

and enzymes called cyclin-dependent kinases (Cdks).
 Each eukaryotic chromosome is one linear DNA double helix

 Average ~108 base pairs long

 With a replication rate of 2 kb/minute, replicating one human

chromosome would require ~35 days.

 Solution ---> DNA replication initiates at many different sites

simultaneously.

Rates are cell

specific!
Replication forks visible in Drosophila
What about the ends (or telomeres) of linear chromosomes?

DNA polymerase/ligase cannot fill gap at end of chromosome after RNA

primer is removed. this gap is not filled, chromosomes would
become shorter each round of replication!

Solution:

1. Eukaryotes have tandemly repeated sequences at the ends of their

chromosomes.

2. Telomerase (composed of protein and RNA complementary to the

telomere repeat) binds to the terminal telomere repeat and
catalyzes the addition of new repeats.

3. Compensates by lengthening the chromosome.

4. Absence or mutation of telomerase activity results in chromosome

shortening and limited cell division.
Synthesis of telomeric DNA by telomerase

Peter J. Russell, iGenetics: Copyright © Pearson Education, Inc., publishing as Benjamin Cummings.
Final Step - Assembly into
Nucleosomes:

 As DNA unwinds, nucleosomes

must disassemble.

 Histones and the associated

chromatin proteins must be
duplicated by new protein
synthesis.

 Newly replicated DNA is

assembled into nucleosomes
almost immediately.

 Histone chaperone proteins

control the assembly.
Transcription Process
 General concepts
• Three phases: initiation, elongation, and
termination.
• The prokaryotic RNA-pol can bind to the DNA
template directly in the transcription process.
• The eukaryotic RNA-pol requires co-factors to bind
to the DNA template together in the transcription
process.
 Transcription of Eukaryotes
A. INITIATION
• Transcription initiation needs promoter and
upstream regulatory regions.
• The cis-acting elements are the specific
sequences on the DNA template that
regulate the transcription of one or more
genes.
 Cis-acting element

cis-acting element
structural gene
GCGC CAAT TATA
exon intron exon

start
TATA box (Hogness box)

enhancer CAAT box

GC box
TATA box
 TRANSCRIPTION FACTORS
• RNA-pol does not bind the promoter directly.
• RNA-pol II associates with six transcription
factors, TFII A - TFII H.
• The trans-acting factors are the proteins that
recognize and bind directly or indirectly cis-
acting elements and regulate its activity.
TF for eukaryotic transcription
 PRE-INITIATION COMPLEX (PIC)
• TBP of TFII D binds TATA
• TFII A and TFII B bind TFII D
• TFII F-RNA-pol complex binds TFII B
• TFII F and TFII E open the dsDNA (helicase and
ATPase)
• TFII H: completion of PIC
 Pre-initiation complex (PIC)

RNA pol II

TBP TAF TF II E
TF II F
TF II
TATA TF II
A B TF II H DNA
 Phosphorylation of RNA-pol

• TF II H is of protein kinase activity to

phosphorylate CTD of RNA-pol. (CTD is the
C-terminal domain of RNA-pol)
• Only the p-RNA-pol can move toward the
downstream, starting the elongation phase.
• Most of the TFs fall off from PIC during the
elongation phase.
B. ELONGATION

• The elongation is similar to that of

prokaryotes.
• The transcription and translation do
not take place simultaneously since
they are separated by nuclear
membrane.
 nucleosome

RNA-Pol

moving
direction

RNA-Pol

RNA-Pol
C. TERMINATION

• The termination sequence is

AATAAA followed by GT repeats.
• The termination is closely related to
the post-transcriptional
modification.
TRANSLATION
 After Transcription
• In prokaryotes, the RNA copy of a gene is messenger RNA, ready to be
translated into protein. In fact, translation starts even before transcription is
finished.
• In eukaryotes, the primary RNA transcript of a gene needs further processing
before it can be translated. This step is called “RNA processing”. Also, it
needs to be transported out of the nucleus into the cytoplasm.
• Steps in RNA processing:
– 1. Add a cap to the 5’ end
– 2. Add a poly-A tail to the 3’ end
– 3. splice out introns.
 Capping
• RNA is inherently unstable, especially at
the ends. The ends are modified to protect
it.
• At the 5’ end, a slightly modified guanine
(7-methyl G) is attached “backwards”, by a
5’ to 5’ linkage, to the triphosphates of the
first transcribed base.
• At the 3’ end, the primary transcript RNA is
cut at a specific site and 100-200 adenine
nucleotides are attached: the poly-A tail.
Note that these A’s are not coded in the
DNA of the gene.
 Introns
• Introns are regions within a gene that don’t code for protein and don’t appear in the final
mRNA molecule. Protein-coding sections of a gene (called exons) are interrupted by introns.
• The function of introns remains unclear. They may help is RNA transport or in control of
gene expression in some cases, and they may make it easier for sections of genes to be
shuffled in evolution. But , no generally accepted reason for the existence of introns exists.
• There are a few prokaryotic examples, but most introns are found in eukaryotes.
• Some genes have many long introns: the dystrophin gene (mutants cause muscular
dystrophy) has more than 70 introns that make up more than 99% of the gene’s sequence.
However, not all eukaryotic genes have introns: histone genes, for example, lack introns.
 Intron Splicing
• Introns are removed from the
primary RNA transcript while it is
still in the nucleus.
• Introns are “spliced out” by
RNA/protein hybrids called
“spliceosomes”. The intron
sequences are removed, and the
remaining ends are re-attached so
the final RNA consists of exons
only.
 Summary of RNA processing
• In eukaryotes, RNA polymerase produces a “primary transcript”, an exact RNA copy of the gene.
• A cap is put on the 5’ end.
• The RNA is terminated and poly-A is added to the 3’ end.
• All introns are spliced out.
• At this point, the RNA can be called messenger RNA. It is then transported out of the nucleus
into the cytoplasm, where it is translated.
 Proteins
• Proteins are composed of one or more polypeptides, plus (in some cases)
additional small molecules (co-factors).
• Polypeptides are linear chains of amino acids. After synthesis, the new
polypeptide folds spontaneously into its active configuration and combines
with the other necessary subunits to form an active protein. Thus, all the
information necessary to produce the protein is contained in the DNA base
sequence that codes for the polypeptides.
• The sequence of amino acids in a polypeptide is known as its “primary
structure”.
 Amino Acids and Peptide Bonds
• There are 20 different amino acids coded
in DNA.
• They all have an amino group (-NH2) group
on one end, and an acid group (-COOH)
on the other end. Attached to the central
carbon is an R group, which differs for
each of the different amino acids.
• When polypeptides are synthesized, the
acid group of one amino acid is attached to
the amino group of the next amino acid,
forming a peptide bond.
 Translation - making proteins
Nuclear
DNA membrane

Transcription
Pre-mRNA

Eukaryotic RNA Processing

Cell mRNA

Ribosome

Translation

Protein
 Translation
• Three parts:
1. initiation: start codon (AUG)
2. elongation:
3. termination: stop codon (UAG)

• Let’s make a PROTEIN!!!!.

 Translation

Large
subunit
P A
Site Site

mRNA
A U G C U A C U U C G

Small subunit
 Translation
• Translation of mRNA into protein is accomplished by the ribosome, an
RNA/protein hybrid. Ribosomes are composed of 2 subunits, large and
small.
• Ribosomes bind to the translation initiation sequence on the mRNA, then
move down the RNA in a 5’ to 3’ direction, creating a new polypeptide. The
first amino acid on the polypeptide has a free amino group, so it is called the
“N-terminal”. The last amino acid in a polypeptide has a free acid group, so it
is called the “C-terminal”.
• Each group of 3 nucleotides in the mRNA is a “codon”, which codes for 1
amino acids. Transfer RNA is the adapter between the 3 bases of the codon
and the corresponding amino acid.
 Transfer RNA
• Transfer RNA molecules
are short RNAs that fold
into a characteristic
cloverleaf pattern. Some of
the nucleotides are
modified to become things
like pseudouridine and
ribothymidine.
• Each tRNA has 3 bases
that make up the anticodon.
These bases pair with the
3 bases of the codon on
mRNA during translation.
 Transfer RNA
• Each tRNA has its
corresponding amino acid
attached to the 3’ end. A set of
enzymes, the “aminoacyl tRNA
synthetases”, are used to
“charge” the tRNA with the
proper amino acid.
• Some tRNAs can pair with more
than one codon. The third base
of the anticodon is called the
“wobble position”, and it can
form base pairs with several
different nucleotides.
 Initiation
aa2
aa1

2-tRNA
1-tRNA
G A U
anticodon U A C

A U G C U A C U U C G A
hydrogen
bonds codon
mRNA
 Initiation of Translation
• In prokaryotes, ribosomes bind to specific translation
initiation sites. There can be several different initiation
sites on a messenger RNA: a prokaryotic mRNA can
code for several different proteins. Translation begins at
an AUG codon, or sometimes a GUG. The modified
amino acid N-formyl methionine is always the first amino
acid of the new polypeptide.
• In eukaryotes, ribosomes bind to the 5’ cap, then move
down the mRNA until they reach the first AUG, the codon
for methionine. Translation starts from this point.
Eukaryotic mRNAs code for only a single gene.
(Although there are a few exceptions, mainly among the
eukaryotic viruses).
• Note that translation does not start at the first base of the
mRNA. There is an untranslated region at the beginning
of the mRNA, the 5’ untranslated region (5’ UTR).
 More Initiation
• The initiation process involves first
joining the mRNA, the initiator
methionine-tRNA, and the small
ribosomal subunit. Several
“initiation factors”--additional
proteins--are also involved. The
large ribosomal subunit then joins
the complex.
 Elongation
• The ribosome has 2 sites for tRNAs, called P and A. The initial
tRNA with attached amino acid is in the P site. A new tRNA,
corresponding to the next codon on the mRNA, binds to the A site.
The ribosome catalyzes a transfer of the amino acid from the P
site onto the amino acid at the A site, forming a new peptide bond.
• The ribosome then moves down one codon. The now-empty
tRNA at the P site is displaced off the ribosome, and the tRNA that
has the growing peptide chain on it is moved from the A site to the
P site.
 Elongation
• The process is then repeated:
– the tRNA at the P site holds the peptide chain, and a new tRNA
binds to the A site.
– the peptide chain is transferred onto the amino acid attached to
the A site tRNA.
– the ribosome moves down one codon, displacing the empty P
site tRNA and moving the tRNA with the peptide chain from the
A site to the P site.
Elongation
 Termination
• Three codons are called “stop
codons”. They code for no
amino acid, and all protein-
coding regions end in a stop
codon.
• When the ribosome reaches a
stop codon, there is no tRNA that
binds to it. Instead, proteins
called “release factors” bind, and
cause the ribosome, the mRNA,
and the new polypeptide to
separate. The new polypeptide
is completed.
• Note that the mRNA continues
on past the stop codon. The
remaining portion is not
translated: it is the 3’
untranslated region (3’ UTR).
Post-Translational Modification
• New polypeptides usually fold themselves
spontaneously into their active
conformation. However, some proteins
are helped and guided in the folding
process by chaperone proteins
• Many proteins have sugars, phosphate
groups, fatty acids, and other molecules
covalently attached to certain amino acids.
Most of this is done in the endoplasmic
reticulum.
 Post-Translational Modification
• Many proteins are targeted to specific organelles
within the cell. Targeting is accomplished
through “signal sequences” on the polypeptide.
In the case of proteins that go into the
endoplasmic reticulum, the signal seqeunce is a
group of amino acids at the N terminal of the
polypeptide, which are removed from the final
protein after translation.
 The Genetic Code
• Each group of 3 nucleotides on the mRNA is a
codon. Since there are 4 bases, there are 43 =
64 possible codons, which must code for 20
different amino acids.
• More than one codon is used for most amino
acids: the genetic code is “degenerate”. This
means that it is not possible to take a protein
sequence and deduce exactly the base
sequence of the gene it came from.
• In most cases, the third base of the codon (the
wobble base) can be altered without changing
the amino acid.
 The Genetic Code
• AUG is used as the start codon. All proteins
are initially translated with methionine in the
first position, although it is often removed after
translation. There are also internal
methionines in most proteins, coded by the
same AUG codon.
• There are 3 stop codons, also called
“nonsense” codons. Proteins end in a stop
codon, which codes for no amino acid.
 More Genetic Code
• The genetic code is almost universal. It is used in both
prokaryotes and eukaryotes.
• However, some variants exist, mostly in mitochondria which have
very few genes.
• For instance, CUA codes for leucine in the universal code, but in
yeast mitochondria it codes for threonine. Similarly, AGA codes
for arginine in the universal code, but in human and Drosophila
mitochondria it is a stop codon.
• There are also a few known variants in the code used in nuclei,
mostly among the protists.
Lesson 6: Genetic Engineering
Lesson 7: Discuss the Applications of Recombinant DNA
Genetic engineering

any process in which an organism’s genome is intentionally altered

does not encompass traditional breeding techniques because it

requires manipulation of an organism’s genes through cloning or
transformation via the addition of foreign DNA

involves the use of molecular techniques to modify the traits of a

target organism
Genetic engineering: 5 Steps

1. Isolation of the genes

2. Insertion of those genes into a transfer vector (a virus or a

plasmid used as a conduit)

3. Transfer of the vector to the organism to be modified

4. Transformation of that organism’s cells

5. Separation of the genetically modified organism (GMO) from

organisms that have not been successfully modified
The modification of traits may involve:

I. introduction of new traits into an organism

II. enhancement of a present trait by increasing the

expression of the desired gene

III. enhancement of a present trait by disrupting

the inhibition of the desired genes’ expression
 A general outline of recombinant DNA may be
given as follows:
I. cutting or cleavage of DNA by restriction enzymes (REs)

II. selection of an appropriate vector or vehicle which would propagate the recombinant DNA ( eg.
circular plasmid in bacteria with a foreign gene of interest)

III. ligation (join together) of the gene of interest (eg. from animal) with the vector ( cut bacterial
plasmid)

IV. transfer of the recombinant plasmid into a host cell (that would carry out replication to make huge
copies of the recombined plasmid)

V. selection process to screen which cells actually contain the gene of interest

VI. sequencing of the gene to find out the primary structure of the protein
Development of Recombinant DNA Technology Began the
Era of Cloning

restriction
DNA
enzymes fragments

• enzymes that cut viral

DNA at specific sites Recombinant
• could be used to cut DNA
any organism’s DNA at
specific nucleotide
DNA
sequences, producing vectors
a reproducible set of
fragments
Genetic Advances in Agriculture
• Improvements in Plants
• Enhanced potential for more vigorous growth and increases yields (hybrid
vigor-heterosis)
• Increase resistance to natural predators and pests, including insects and
disease-causing microorganisms
Genetic Advances in Agriculture

• Production of hybrids exhibiting a

combination of superior traits derived
from two different strains or even
two different species
• Pluot (plum+apricot)
• Tangelo (tangerine +
grapefruit/pomelo)
• Tayberry (blackberry+raspberry)
• Rabbage (cabbage+radish)
Genetic Advances in Agriculture

• Selection of genetic variation with desirable qualities

• increased protein value
• Increased content of limiting amino acids
• Smaller plant size, reducing vulnerability to adverse weather conditions
Genetic Advances in Agriculture

• Improvements in Animals
• Development of superior breeds in livestock
• Chickens
• Grow faster
• Produce more high-quality meat
• Lay greater number of egg
• Larger animals (pig and cow)
• Artificial insemination
• Sperm form a single male with superior genetic trait used to
fertilize thousands of females
Genetic Advances in Medicine

Genetic basis of disorders are documented

Advances in cancer research

• Effective early detection and more effective approaches to treatment

Genetic Counseling
• Provides couples with objective information on which they can base rational
decisions about child-bearing
Immunogenetics
• Compatible blood transfusions and organ transplants
 • Recombinant DNA techniques
DNA • Manipulating & cloning a variety of genes
biotechnology (medically important molecules: insulin,
blood clotting factors, growth hormone)

• Genetic disorders are treated by inserting

Gene therapy normal copies of genes into cells of
afflicted individuals

Human
• The entire genetic complement (genome)
Genome of several species is being sequenced
Project
Chapter 4: Compare and Contrast
Processes in Plants and Animals
Chapter 4: Compare and Contrast Processes
in Plants and Animals
Lesson 17: Reproduction and Development

Lesson 18: Nutrition

Lesson 19: Gas Exchange

Lesson 20: Transport and Circulation

Lesson 21: Regulation of Body Fluids

Lesson 22: Immune Systems

Lesson 23: Chemical and Nervous Control

Lesson 24: Sensory and Motor Mechanisms

Lesson 25: Feedback Mechanisms

Lesson 17: REPRODUCTION AND DEVELOPMENT
Reproductive Systems
Male & Female
Function
 To ensure survival of the species
 To produce egg and sperm cells
 To transport and sustain these cells
 To nurture the developing offspring

 To produce hormones

 Other systems strive to maintain a state of

homeostasis
Male & Female
 Include:
 Reproductive organs called gonads that
produce gametes (reproductive cells) and
hormones
 Ducts that transport gametes
 Accessory glands and organs that secrete
fluids
 MALE
 Functions
 To produce, maintain and transport sperm
(the male reproductive cells) and protective
fluid (semen)
 To produce and secrete male sex hormones
responsible for maintaining the male
reproductive system
 Male
 Consists of:
 A pair of testes
 A network of excretory ducts
 epididymis
 vas deferens
 ejaculatory ducts
 Seminal vesicles
 Prostate
 Urethra
 Penis
 Female
 Functions
 Produces the female egg cells
 Transports the eggs to the site of fertilization
 The fertilization of an egg by a sperm,
occurs in the fallopian tubes
Female
 Functions
 After fertilization, provides a place
for a baby to develop
 If fertilization does not take place,
the system is designed to
menstruate
 Produces female sex hormones that
maintain the reproductive cycle.
EXTERNAL GENITALIA

 The vulva refers
Individual to those parts
differences in: that are outwardly visible
 The vulva includes:
 Size
 Mons pubis

 Coloration
Labia majora

 Shape
Labia minora

 Clitoris
Of external genitalia are common
 Urethral opening
 Vaginal opening
 Perineum
INTERNAL GENITALIA
Vagina

Cervix

Uterus

Fallopian Tubes

Ovaries
http://www.ricancercouncil.org/img/cervical.gif
 Assessment Techniques
 Cystoscopy
 Prostate-specific antigen (PSA)
 Pap smear
 Mammography
 Amniocentesis
 Ultrasonography
 Chorionic villus sampling (CVS)
 Self-examinations

Copyright 2003 by Mosby, Inc. All rights reserved.

Disorders of the Reproductive System
 Cancer  Gonorrhea
 Chancroid  Herpes simplex virus
 Chlamydia  AIDS/HIV
 Cryptorchidism  Premenstrual syndrome (PMS)
 Ectopic pregnancy  Pubic lice
 Genital warts  Syphilis
 ISSUES AND INNOVATIONS
• Alternatives in conception
– Birth control
– In vitro fertilization
– Surrogacy
• Infertility
– One out of every six couples is infertile
– Factors include low sperm count, STDs, and stress
• Redefining the sexes
– Homosexual and bisexual
Evaluation of contraceptive
methods
 Do they work?
 Are they safe?
 Are they available?
 Are they affordable?

 Do they protect against sexually transmitted

diseases (STDs)?
 ABSTINENCE

Surgical sterilization
essentially permanent
Animal Reproduction and Development
Animals reproductive by asexual and sexual methods:
•Asexual is the production of offspring with genes all from one
individual, without the fusion of gametes.
•Sexual involves formation of gametes and fertilization, genetic
variation
Asexual Methods Include:

Budding in Hydra Starfish Regeneration

Gemmules in Sponges
Parthenogenesis: an unfertilized egg develops

Honey bees: drones are males and are produced

parthenogeneticly and female workers and queens form from
fertilized eggs.

Queen Worker (female) Drone (male)

Bee
reproduction
Sexual Reproduction
Hermaphroditism: individual has both male and female
reproductive systems
Earthworm
Fertilization can occur externally or internally
Aquatic animals tend to be external
Terrestrial animals tend to be internal
Development can be external in the water, external on land,
or internal.
Oviparous: lay eggs, Amniotic eggs are terrestrial eggs
Ovoviparous: live birth from eggs (some sharks and
snakes)
Viviparous: live placental birth
Lesson 18: NUTRITION
 Nutrition
Process by which organisms obtain and utilize their food.

There are two parts to Nutrition:

1. Ingestion- process of taking food into the
digestive system so that it may be hydrolized
or digested.
2. Digestion- the breakdown of food (either
chemically or mechanically) in order to utilize
nutrients
 Types of Nutrients
• Micronutrients- vitamins, minerals, & water

• Macronutrients- proteins, lipids,

carbohydrates, etc…
Human digestive
system
GI (gastrointestinal) tract = alimentary canal
 Swallowing (& not choking)

• Epiglottis
– flap of cartilage
– closes trachea (windpipe) when swallowing
– food travels down esophagus
• Peristalsis
– involuntary muscle contractions to move food along
 Which type of digestion is the
following?

1. Chewing a saltine? -

2. Saliva breaking the saltine down into molecules of

glucose? -

3. Your tongue breaking pieces of a hamburger apart?

4. Pepsin (an enzyme) in your stomach breaking the

hamburger into amino acids?
Digestive Homeostasis Disorders
• ULCERS – erosion of the surface of
the alimentary canal generally
associated with some kind of irritant
Digestive Homeostasis Disorders
• CONSTIPATION – a
condition in which the
large intestine is emptied
with difficulty.
• Too much water is
reabsorbed
• and the solid waste
hardens
 Digestive Homeostasis
Disorders
• DIARRHEA – a gastrointestinal
disturbance characterized by
decreased water absorption and
increased peristaltic activity of
the large intestine.
• This results in increased,
multiple, watery feces.
• This condition may result in
severe dehydration, especially in
infants
 Digestive Homeostasis
Disorders

• APPENDICITIS – an inflammation of the appendix due

to infection
• Common treatment is removal of the appendix via
surgery
 Digestive Homeostasis
Disorders

• GALLSTONES – an accumulation of
hardened cholesterol and/or calcium
deposits in the gallbladder
• Can either be “passed” (OUCH!!) or
surgically removed
 Digestive Homeostasis
Disorders

• ANOREXIA NERVOSA - a psychological

condition where an individual thinks they
appear overweight and refuses to eat.
• Weighs 85% or less than what is
developmentally expected for age and
height
• Young girls do not begin to menstruate at
the appropriate age.
 Digestive Homeostasis
Disorders

• HEART BURN – ACID from the

stomach backs up into the esophagus.
Lesson 19: GAS EXCHANGE
Respiratory System
HA LE
E…E X
L
INHA
Latin systema respiratorium

The respiratory system (or

ventilatory system) is the
biological system of an organism
that introduces respiratory
gases to the interior and
performs gas exchange.
oIn humans and other mammals, the anatomical
features of the respiratory system include
airways, lungs, and the respiratory muscles.

oOther animals, such as insects, have respiratory

systems with very simple anatomical features,
and in amphibians even the skin
plays a vital role in gas exchange.
oPlants also have respiratory systems
but the directionality of gas exchange
can be opposite to that in animals.

oThe respiratory system in plants also

includes anatomical features such as
holes on the undersides of leaves
known as stomata.
oProvide a means of getting oxygen/O2
out of the air and into the vicinity of
the cell so that can
be utilized.

oElimination of
carbon dioxide/CO2.
 Respiration VS Breathing
• Respiration: • Breathing:
• The actual burning of • Merely part of
the food in the cells,
the mechanism by
release of energy and
the waste product;
which cells obtain
carbon dioxide/CO2. oxygen/O2.
 Structures designed to remove oxygen
from the air or H2O and pass it to some
sort of transport system:

• Lungs, gills and other structures.

Note:
• All animals from amoeba to man respire
the same way at the cellular level.

• Methods employed by animals to bring O2

to the cells are highly varied and become
extremely complex to large animals.
Ventilation

In respiratory physiology, ventilation (or

ventilation rate) is the rate at which gas enters
or leaves the lung. It is categorized under the
following definitions:
 Measurement Description
The total volume of gas
Minute ventilation entering the lungs per
minute.
The volume of gas per unit
time that reaches the
Alveolar ventilation alveoli, the respiratory
portions of the lungs where
gas exchange occurs.
The volume of gas per unit
time that does not reach
these respiratory portions,
Dead space ventilation
but instead remains in the
Inhalation

oInhalation is initiated by the diaphragm and

supported by the external intercostal
muscles.

oNormal resting respirations are 10 to 18

breaths per minute, with a time period of 2
seconds. During vigorous inhalation (at rates
exceeding 35 breaths per minute), or in
approaching respiratory failure, accessory
muscles of respiration are recruited for
support.
Exhalation

oThe lungs have a natural elasticity: as they

recoil from the stretch of inhalation, air
flows back out until the pressures in the chest
and the atmosphere reach equilibrium.

oDuring forced exhalation, as when blowing

out a candle, expiratory muscles including the
abdominal muscles and internal intercostal
muscles, generate abdominal and thoracic
pressure, which forces air out of the lungs.
Gas exchange
oThe major function of the respiratory system is
gas exchange between the external environment
and an organism's circulatory system.

oIn humans and other mammals, this exchange

facilitates oxygenation of the blood with a
concomitant removal of carbon dioxide and other
gaseous metabolic wastes from the circulation.

oAs gas exchange occurs, the acid-base balance

of the body is maintained as part of homeostasis.
 Vocalization
oThe movement of gas through the larynx, pharynx
and mouth allows humans to speak, or phonate.
Vocalization, or singing, in birds occurs via the
syrinx, an organ located at the base of the trachea.

oThe vibration of air

flowing across the
larynx (vocal cords), in
humans, and the syrinx,
in birds, results in
sound.
Temperature control

oPanting in dogs, cats and some other animals

provides a means of controlling body temperature.
Coughing and sneezing

oIrritants caught in the mucus which lines the

respiratory tract are expelled or moved to the
mouth where they can be swallowed.

oDuring coughing, contraction of the smooth muscle

narrows the trachea by pulling the ends of the
cartilage plates together and by pushing soft tissue
out into the lumen.
Coughing and sneezing

oThis increases the expired airflow rate to

dislodge and remove any irritant particle or
mucus.
These are summarized in Table below
Different Modes Of Respiration in aerobic animals
Modes of Respiration Respiratory Organs Animals
1. Body surface Body surface Sponges and
respiration coelenterates ,
(examples hydra)

2. Bronchial Gills Crustacean (example:

respiration Prawn) mollusks and
fishes.

3. Cutaneous Skin Annelid (for examples

respiration leech, earthworm) and
amphibians
4. Tracheal Trachea Insects (Examples:
(intercomunicating Cockroach),
respiration tubes) centipedes, millipedes.

5. Book-lung Book-lungs (book like Arachnids (example:

structures) Spider, Scropion)
respiration

6. Buccopharyngeal cavity Frogs and toads

Buccopharyngeal
respiration

7. Pulmonary Lungs Amplibians, reptiles,

birds, mammals
respiration
Lungs
Horses

Horses are obligate

nasal breathers which
means that they are
different from many
other mammals
because they do not
have the option of
breathing through
their mouths and must
take in oxygen
through their noses.
Laryngitis - Swelling and irritation of the larynx

Bronchitis - An infection in your bronchial tubes

Tonsillitis - Inflammation of the tonsils

Pneumonia - An infection deep in your

lungs

Bronchiectasis - Widening and

destruction of the airways, often
caused by recurrent infection
or inflammation
Influenza (the flu) - A virus that enters your body
through your nose and mouth and causes fever,
headaches, muscle aches and sore throat

Asthma - A chronic disease in which your airway

walls become sore and swollen, narrowing so that
your lungs get less air

Tuberculosis (TB) - A bacterial

Infection that attacks the lungs and
other parts of the body and can be
deadly if not treated correctly
Lung cancer - One of the most common
cancers in the world, usually caused by
cigarette smoke

Chronic obstructive pulmonary disease

(COPD) - Damage of the airways and
air sacs from cigarette smoke and
other pollutants that prevents
airflow in and out of your lungs
Emphysema - A type of COPD, usually
caused by cigarette smoke, which makes it
hard to catch your breath

Pulmonary embolism (PE) - A blood clot

that blocks a lung artery because of
surgery or long periods of inactivity
Prevention and Treatment of Respiratory Disease - Asthma.

oRemove allergens from the home, including dust, dust mites, cleaning
chemicals, pets and carpets.

oEncase mattresses and box springs in allergen-proof products.

oWash all linens, blankets, quilts and comforters at least once a week in
hot water.

oUse only allergen-proof pillows and blankets.

oClean the home thoroughly and often.

Prevention and Treatment of Respiratory
Disease - Sinusitis.

oAvoid cigarette smoke and pollution;

oHumidifiers;

oIncreased liquids;

oTreat allergies and respiratory infections promptly

oSinus surgery to drain sinus cavities;

Myth: Asthma is caused by emotions or
psychological conditions.

Fact: Emotions may exacerbate asthma, but the

hypersensitive respiratory airways were present
before emotions were introduced.
Myth: A child's asthma is not caused by lack of a
strong bond with the mother.
Fact: No scientific evidence has ever existed to
support this.

Myth: Asthma is psychosomatic and should be

treated by psychiatrists or psychologists.
Fact: Asthma and allergies are physical
conditions of physical hypersensitivity
of the respiratory system. Asthmatics
do not cause their own asthma attacks.
Lesson 20: TRANSPORT AND CIRCULATION
Functions of the Circulatory System

Serves for the Fights Acid-Base

transport of: infection balance

nutritive
products

respiratory
materials

hormones

excretory
products
The circulatory system is made up of the:

blood vascular
system

lymphatic
system
Blood Vascular System

blood • a system of
vessels channels

• the propulsive
heart organ

• the circulating
blood medium
Open Close
Type d Type
Types of
circulatory
system
OPEN CIRCULATORY SYSTEM
 well-developed blood vessels which pass from the
heart to the body tissues
 blood vessels open into spaces (lacunae)
 from the intercellular spaces, blood returns to the
heart
CLOSED CIRCULATORY SYSTEM
blood confined in tubes throughout its course from
the heart
blood keeps flowing rapidly but requires an
auxiliary lymphatic system
more efficient for animals of large size and active
habits
The circulatory system penetrates almost
every part of the body except the:

epidermis of the skin

cornea of the eye

cartilage tissue

enamel of the teeth

feathers
Blood Vascular
System
 BLOOD VESSELS

 Capillaries
 Arteries

 Veins
BLOOD VESSELS

Capillaries

no walls
usually
except
smallest found within
endotheliu
the organ
m
BLOOD VESSELS

oxygenated
flow of
blood away walls
blood is deeply set no valves
from the athicker
pulsating
heart

Arteries
BLOOD VESSELS

Veins

unoxygenate
flow of blood
d blood walls are superficially
is smooth with valves
towards the thinner set
and slow
heart
HEART
The main pumping or propulsive organ of the
body
weighs about 9-11 ounces in human males,
being lighter in females
made up of chambers, the atria (auricles) and
the ventricles
 The Heart
• The heart is a cone-shaped, muscular
organ located between the lungs behind
the sternum.
• The heart muscle forms the myocardium,
with tightly interconnect cells of cardiac
muscle tissue.
• The pericardium is the outer membranous
sac with lubricating fluid.
• The heart has four chambers: two upper, thin-
walled atria, and two lower, thick-walled
ventricles.
• The septum is a wall dividing the right and
left sides.
• Atrioventricular valves occur between the
atria and ventricles – the tricuspid valve on
the right and the bicuspid valve on the left;
both valves are reinforced by chordae
tendinae attached to muscular projections
within the ventricles.
• Valvular stenosis
External heart anatomy
Coronary artery circulation
TYPES OF HEART

TWO- THREE- FOUR-

CHAMBERED CHAMBERED CHAMBERED
 Passage of Blood Through the
Heart
• Blood follows this sequence through the heart:
superior and inferior vena cava → right atrium
→ tricuspid valve → right ventricle →
pulmonary semilunar valve → pulmonary trunk
and arteries to the lungs → pulmonary veins
leaving the lungs → left atrium → bicuspid
valve → left ventricle → aortic semilunar valve
→ aorta → to the body.
Copyright 2003 by Mosby, Inc. All rights reserved.
 Blood Flow
• The beating of the heart is necessary to
homeostasis because it creates pressure
that propels blood in arteries and the
arterioles.
• Arterioles lead to the capillaries where
nutrient and gas exchange with tissue
fluid takes place.
 Blood Flow in Arteries
• Blood pressure due to the pumping of the
heart accounts for the flow of blood in the
arteries.
• Systolic pressure is high when the heart
expels the blood.
• Diastolic pressure occurs when the heart
ventricles are relaxing.
• Both pressures decrease with distance from
the left ventricle because blood enters more
and more arterioles and arteries.
Cross-sectional area as it relates to
blood pressure and velocity
 Blood Flow in Capillaries

• Blood moves slowly in capillaries

because there are more capillaries than
arterioles.
• This allows time for substances to be
exchanged between the blood and tissues.
 Blood Flow in Veins
• Venous blood flow is dependent upon:
1) skeletal muscle contraction,
2) presence of valves in veins, and
3) respiratory movements.
• Compression of veins causes blood to
move forward past a valve that then
prevents it from returning backward.
• Changes in thoracic and abdominal pressure
that occur with breathing also assist in the
return of blood.
• Varicose veins develop when the valves of
veins become weak.
• Hemorrhoids (piles) are due to varicose veins
in the rectum.
• Phlebitis is inflammation of a vein and can
lead to a blood clot and possible death if the
clot is dislodged and is carried to a pulmonary
vessel.
 Cardiovascular Disorders

• Cardiovascular disease (CVD) is the leading

cause of death in Western countries.
• Modern research efforts have improved
diagnosis, treatment, and prevention.
• Major cardiovascular disorders include
atherosclerosis, stroke, heart attack, aneurysm,
and hypertension.
 Atherosclerosis
• Atherosclerosis is due to a build-up of fatty
material (plaque), mainly cholesterol, under
the inner lining of arteries.
• The plaque can cause a thrombus (blood clot)
to form.
• The thrombus can dislodge as an embolus
and lead to thromboembolism.
 Stroke, Heart Attack, and
Aneurysm
• A cerebrovascular accident, or stroke, results
when an embolus lodges in a cerebral blood
vessel or a cerebral blood vessel bursts; a
portion of the brain dies due to lack of oxygen.
• A myocardial infarction, or heart attack,
occurs when a portion of heart muscle dies due
to lack of oxygen.
• Partial blockage of a coronary artery causes
angina pectoris, or chest pain.
• An aneurysm is a ballooning of a blood vessel,
usually in the abdominal aorta or arteries
leading to the brain.
• Death results if the aneurysm is in a large
vessel and the vessel bursts.
• Atherosclerosis and hypertension weaken
blood vessels over time, increasing the risk of
aneurysm.
 Coronary Bypass Operations

• A coronary bypass operation involves

removing a segment of another blood vessel
and replacing a clogged coronary artery.
• It may be possible to replace this surgery
with gene therapy that stimulates new blood
vessels to grow where the heart needs more
blood flow.
Coronary bypass operation
 Clearing Clogged Arteries
• Angioplasty uses a long tube threaded
through an arm or leg vessel to the point
where the coronary artery is blocked;
inflating the tube forces the vessel open.
• Small metal stents are expanded inside the
artery to keep it open.
• Stents are coated with heparin to prevent
blood clotting and with chemicals to
prevent arterial closing.
Angioplasty
 Dissolving Blood Clots
• Medical treatments for dissolving blood clots
include use of t-PA (tissue plasminogen
activator) that converts plasminogen into
plasmin, an enzyme that dissolves blood clots,
but can cause brain bleeding.
• Aspirin reduces the stickiness of platelets and
reduces clot formation and lowers the risk of
heart attack.
 Heart Transplants and Artificial
Hearts
• Heart transplants are routinely performed
but immunosuppressive drugs must be taken
thereafter.
• There is a shortage of human organ donors.
• Work is currently underway to improve self-
contained artificial hearts, and muscle cell
transplants may someday be useful.
 Hypertension
• About 20% of Americans suffer from
hypertension (high blood pressure).
• Hypertension is present when systolic pressure
is 140 or greater or diastolic pressure is 100 or
greater; diastolic pressure is emphasized when
medical treatment is considered.
• A genetic predisposition for hypertension occurs
in those who have a gene that codes for
angiotensinogen, a powerful vasoconstrictor.
Lesson 21: REGULATION OF BODY FLUIDS
 Major Parts of the Machine
food, water intake oxygen intake Based on: Starr, C., Biology:
Concepts and Applications,
Brooks/Cole

elimination
Digestive System Respiratory System of carbon
dioxide
nutrients, oxygen
water, carbon
salts dioxide

Circulatory System
Urinary System
water
solutes

elimination rapid transport elimination of

of food to and from all excess water
residues living cells salts, wastes
 Overview of Lesson
• Formation of urine
• Urinary system
• Water balance and alcohol
• Problems of the urinary
tract
When protein is broken down
in the body, it results in
nitrogenous waste that must be
eliminated from the body
 Protein

Based on: Mader, S., Inquiry Into Life, McGraw-Hill

 Examples
of Amino
Acids

Based on: Mader, S., Inquiry Into Life, McGraw-Hill

All 20 amino acids have a nitrogen group (NH2).

When broken down for energy, the nitrogen group
is converted to ammonia (NH3).
 Circulatory
System
Ammonia is
converted into urea
by the liver. Urea is
then transported in
the blood to the
kidneys where the
urea is removed
from the blood.
Based on: Mader, S., Inquiry Into Life
Urea is less toxic than ammonia
and can be transported in the
blood to the kidney
O
H2N - C - NH2
urea
 Formation of Urine
Amino acids in protein are broken down,
resulting in production of ammonia

Ammonia is converted to urea in liver

Urea travels in blood to kidneys, where

removed from blood and incorporated
into urine
 Urinary System

Based on: Mader, S., Inquiry Into Life, McGraw-Hill

 Anatomy of
the Kidney

Based on: Mader, S., Inquiry Into Life, McGraw-Hill

 Urine Formation by Nephron
Blood pressure forces water, glucose,
amino acids and urea from capillaries into
nephron

Glucose and amino acids are reabsorbed

into blood from nephron

Some water is reabsorbed into blood

Urine is urea and salt concentrated in water

 Nephron

Based on: Mader, S., Inquiry Into Life, McGraw-Hill

 Urine moves from the
collecting ducts through the
kidney pelvis to the ureter

Based on: Mader, S., Inquiry Into Life, McGraw-Hill

 Each kidney contains over 1 million nephrons and
thousands of collecting ducts
Glomerulus DCT
renal
cortex

PCT
renal
medulla
Collecting duct
Loop of Henle
 efferent
arteriole afferent
arteriole

Glomerular
Filtration

Bowman’s
capsule glomerulus

Filters blood; proteins can’t pass through

Composition of Glomerular Filtrate

Water

Small Soluble Organic Molecules

Mineral Ions
Proximal Convoluted Tubule

Reabsorbs: water, glucose,

amino acids, and sodium.
• 65% of Na+ is reabsorbed
• 65% of H2O is reabsorbed
• 90% of filtered bicarbonate (HCO3-)
• 50% of Cl- and K+
 Loop of Henle

Creates a gradient of increasing sodium ion

concentration towards the end of the loop
within the interstitial fluid of the renal
pyramid.

25% Na+ is reabsorbed in the loop

15% water is reabsorbed in the loop

40% K is reabsorbed in the loop

 Distal Convoluted Tubule

Under the influence of the

hormone aldosterone, reabsorbs
sodium and secretes potassium.
Also regulates pH by secreting
hydrogen ion when pH of the
plasma is low.
• only 10% of the filtered NaCl and 20% of
water remains
 Collecting Duct

Allows for the osmotic

reabsorption of water.
ADH (antidiuretic hormone)- makes collecting
ducts more permeable to water-- produce
concentrated urine
Urine moves from the kidneys,
through the ureters to the bladder
and finally through the urethra

Based on: Mader, S., Inquiry Into Life, McGraw-Hill

 Urinary Bladder

ureters
internal
external sphincters
sphincters
urethra
 Bladder

1. Mucosa (transitional epithelium)

2. Muscular layer (detrusor muscle): 3
layers of smooth muscle
3. Fibrous adventia
Sphincter Muscles on Bladder

Internal urethral sphincter:

• Smooth muscle
• Involuntary control
• More superiorly located

External Urethral sphincter:

• Skeletal muscle
• Voluntary control
• Posteriorly located
Diuresis (Micturition)

When bladder fills with 200 ml of urine,

stretch receptors transmit impulses to the
CNS and produce a reflex contraction of the
bladder (PNS)

When is incontinence normal?

Distension
of the
Urinary
Bladder
 Regulation of Water Balance
Brain monitors water content of blood

If low water content, pituitary releases ADH

ADH travels in blood to nephron

ADH causes more water to move from urine

back into blood
 Nephron

Based on: Mader, S., Inquiry Into Life, McGraw-Hill

Alcohol consumption
suppresses the production of
ADH by the pituitary.
Why would this result in
dehydration and a hangover?
 Incontinence (urine leakage)
• More than 10 million Americans experience
incontinence

• Most do not seek treatment

• Treatment can improve or eliminate the

problem 90% of the time
 Causes of Incontinence

Stress incontinence: leaking small amounts

of urine when coughing, lifting, or exercising

Urge incontinence: the bladder suddenly and

unexpectedly contracts and expels urine

Overflow incontinence: bladder cannot

completely empty so urine dribbles
 Treatments for Incontinence
• Kegel exercises to strengthen the urinary
sphincter
• Medicines that increase the sphincter’s ability
to contract
• Surgery to strengthen the pelvic muscles or to
lift the bladder
• Retrain the bladder to increase its storage
capacity (allowing 3-4 hours between urinating)
• Drugs to prevent urge incontinence
• Surgery to remove part of prostate gland if
responsible for overflow incontinence
Kidney stones form in the kidney
pelvis. There are 4 types of stones.

•Calcium stones
(most common)
•Uric acid stones
•Bacteria caused stones
•Cystein stones

Based on Mayo Clinic Health Letter

Kidney stones cause pain when
they pass down the ureters to the
bladder and urethra

Based on: Mader, S., Inquiry Into Life, McGraw-Hill

 Treatments for Kidney Stones
• Small stones may pass with no pain
• Larger stones may pass but cause extreme of
pain, requiring a lot pain medication
• Stones that are too large to pass may require
surgical treatment including:
– using a ureteroscope to go up and snare the
stone
– using a nephroscope to crush the stone and
retrieve it
– using shock wave lithotripsy where a person
is submerged in water containing shock
waves to pulverize the stones
 Urinary Tract Infection (UTI)
• Second most common infection following
respiratory infections

• UTI occur when bacteria (E. coli) from the

digestive tract get into the opening of the
urinary tract and multiply

• Bacteria first infect the urethra, then move to

the bladder and finally to the kidneys

• UTI tend to occur more in women than men

Women may have more UTIs than men because:
1) they have a shorter urethra, allowing quicker
access to the bladder
2) the urethral opening is nearer the anus
3) intercourse may result in UTIs in women

Based on: Harvard Medical School Family Health Guide

 Symptoms of UTIs
• Urge to urinate but only small
amount of urine produced
• Pain and burning sensation in
bladder
• Fever
• Blood in urine
 Diagnosis and Treatment

• Doctors check urine for white and red

blood cells and bacteria
• Bacteria grown in culture to
determine which antibiotic will work
the best
• UTIs are treated with antibiotics and
are often cured within 1 or 2 days
Kidney Disease and Ethnicity
• Kidney disease 26 million Americans

• African Americans five times likely to

require dialysis or kidney transplant
• Possible reasons
– Lack of health care
– Genetic component
– Diabetes
– Hypertension
Lesson 25: FEEDBACK MECHANISMS
REGULATING THE INTERNAL ENVIRONMENT

Most cells are NOT in contact with the external environment

Most cells CANNOT function without other cells

Most cells are in contact with the internal environment

The internal environment consists of material outside the cell, but inside the body

Define ExtraCellular Fluid (ECF)

Define IntraCellular Fluid (ICF)

Define InterStitial Fluid (ISF)

• 60% of the adult human body is fluid,mainly a water
solution of ions and other substances
• most of this fluid is inside the cells and is called
intracellular fluid
• one third is in the spaces outside the cells and is called
extracellular fluid
– in constant motion throughout the body
– transported rapidly in the circulating blood and then mixed
between the blood and the tissue fluids by diffusion through the
capillary walls
• The internal environment of vertebrates is called the
interstitial fluid
Components of
the
ExtraCellular
Fluid
Internal environment
• Claude Bernard (1813-1878)
– made the distinction between
external environments surrounding
an animal and the internal
environment in which the cells of the
animal actually live
– French physiologist
– Le milieu interieur
• Walter B. Canon (1871-1945)
– Coined the term Homeostasis
– Sympathetic “flight or fight”
response
Homeostasis
• Cells can live and function only when
they are bathed by ECF that is
compatible with their survival
• The cell must obtain nutrients and
discharge waste to the ECF
Homeostasis
• “steady state”
– internal balance
• dynamic state
– an interplay between outside forces that tend to
change the internal environment and internal
control mechanisms that oppose such
changes.
02/22/2021
Homeostasis
• most organ systems contribute to
homeostasis
– maintenance of a constant internal environment
in spite of constant change
• provides for material needs of cells
• removes wastes from cells
• regulates physical environment of cells
• communicates among cells
Factors affecting Homeostasis
• Amount of energy-rich molecule - fuel
• O2 & CO2 concentration - for chemical reactions
• Waste products - toxicity
• pH -acid/base balance, enzymatic activity
• Water, salt & electrocytes - cell size
• Volume & pressure
• Temperature - narrow range
• Social parameters -social insects (termites)
Control system
• Any homeostatic
control system has
three functional
components: a
receptor, a control
center, and an effector.
• Feedback circuits
– • Negative
– • Positive

02/22/2021
Feedback control systems
• the regulatory proceses that maintain homeostasis
in cells and multicellular organisms
• occurs when sensory information about a
particular variable (e.g. temperature, salinity, pH) is
used to control processes in cells, tissues, and
organs that influence the internal level of this
variable
Feedback-Control Systems
• Conformers
animal’s internal changes parallel the external
conditions
unable to maintain homeostasis for internal
conditions like body dluids salinity or tissue
oxygenation
osmofonformers
• e.g. starfish Asteria-body fluid salinity;
oxyconformers
• annelid worms O2 consumption
Feedback-Control Systems
• Regulators
 animal defend a relatively constant state
 use biochemical, physiological, behavioral, and other mechanisms to
regulate their internal environment over abroad range of exernal
environmental change to maintain homeostasis
 osmoregulators
• maintain ion concentrations of body fluids above environmental levels when placed
in dilute water and below environmental levels when placed in concentrated water
 oxyregulators
• crayfish, most mollusks, almost all vertebrates
• maintain their oxygen consumption at near-steady levels as environemntal oxygen
availability falls
Feedback-Control Systems
• Avoiders
– minimize internal variations by avoiding
environmental disturbances
– some fish avoid temperature changes by changing
location
• Enantiostasis (allostasis)
– change in one physiological variable to counteract a
change in another
– Blue crabs -change blood pH to increase O2 binding
when in brackish water
Homeostasis
• homeostatic regulatory components
– controlled systems - effectors
– regulatory systems
• acquire information
• process information
• integrate information
• send commands
Homeostasis
• homeostatic regulatory variables
– setpoint
• optimal chemical or physical condition
– feedback information
• actual current condition
– error signal
• discrepancy between setpoint and feedback value
Homeostasis
• homeostatic regulatory inputs
– negative feedback
• reduces or reverses activity of effector
• returns condition to set point
– positive feedback
• amplifies activity of effector
• self-limiting activities
– feedforward information
• changes setpoint
Negative Feedback
• if some factor becomes excessive or
deficient, a control system initiates
negative feedback, which consists of a
series of changes that return the factor
toward a certain mean value, thus
maintaining homeostasis
02/22/2021 384
Feedback effectors
• Antagonistic control:
– Opposes change in the variable
– Temperature falls -> effectors produce change to increase
temperature (thermostatic effect)
• Behaviors as effectors:
– Animal seeks out a different location (Avoiders)
– Migration of Monarch butterflies to avoid the cold north
– Killfish seeks out water to keep its internal salinity about 35to
45% that of normal seawater
Enhancement & expansion of basic negative
feedback systems
Antagonistic effectors Feedforward
• Flaws of Negative Feedback Systems
– ▸It must first suffer a disturbance before it can act
– ▸Delayed response
– ▸Overshoots the set point
• Flaws Overcome by:
– Feedforward system (Anticipation)
– ♦Predicts results of a disturbance
– ♦Prevents overshooting of the set point
– e.g. increased insulin secretion while meal is still in
digestive tract
– Acclimatization systems
Adaptation, Acclimatization & Acclimation
• Adaptation
– evolution by natural selection
• Acclimatization
– physiological, biochemical, or anatomical change within
an individual from chronic exposure to a new
environment
• Acclimation
– same as above, but induced experimentally
Other Controlled Systems
• Rheostasis
• non-homeostatic regulation
• ♦Reset system
– changes the setpoint temporarily, permamently or cyclically
– Fever
– Sex hormone concentrations -at puberty
– Reproductive cycle -menstrual cycle
• ♦Positive feedback system
– Creates rapid change
– Reinforces the change in the same direction
– e.g. neuron action potentials, lactation, blood clotting,
orgasms
Mechanisms for regulated change
Mechanisms for regulated change
Positive Feedback
• is generally used to produce a regenerative, explosive, or
autocatalytic effect
• used to generate the rising phase of a cyclic event
– upstroke of nerve impulse
– explosive growth of a blood clot to prevent blood loss
– rapid emptying of body cavity (expulsion of fetus from uterus, vomiting,
swallowing)
• most often encountered in pathological conditions that affect
normal negative feedback control
• One might ask the question, Why do
essentially all control systems of the body
operate by negative feedback rather than
positive feedback?
– If one considers the nature of positive
feedback,one immediately sees that positive
feedback does not lead to stability but to
instability and often death
Positive feedback
• better known as a “vicious cycle” but
a mild degree of positive feedback can
be overcome by the negative
feedback control mechanisms of the
body, and the vicious cycle fails to
develop
Positive Feedback Can Sometimes Be Useful
• Childbirth
• Generation of nerve signals
• Blood clotting
– when a blood vessel is ruptured and a clot begins to form
– multiple enzymes called clotting factors are activated within the clot
itself
– some of these enzymes act on other unactivated enzymes of the
immediately adjacent blood, thus causing more blood clotting
– process continues until the hole in the vessel is plugged and bleeding
no longer occurs