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Quipment: Equipment and Supplies Used in Venipuncture

This document discusses equipment, supplies, and procedures used for venipuncture. It describes the necessary materials including blood collection stations, chairs, antiseptics, needles, tube holders, and evacuated blood collection tubes. Different types of needles and tube systems are outlined. Color coding of tube caps indicates additives that prevent clotting or preserve blood components. Proper order of draw is also important to avoid contamination between sample types.

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Vera June Rañeses
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284 views

Quipment: Equipment and Supplies Used in Venipuncture

This document discusses equipment, supplies, and procedures used for venipuncture. It describes the necessary materials including blood collection stations, chairs, antiseptics, needles, tube holders, and evacuated blood collection tubes. Different types of needles and tube systems are outlined. Color coding of tube caps indicates additives that prevent clotting or preserve blood components. Proper order of draw is also important to avoid contamination between sample types.

Uploaded by

Vera June Rañeses
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd
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EQUIPMENT AND SUPPLIES USED IN VENIPUNCTURE

  Selecting the correct tools and using them properly


during the testing procedure ensures not only safe collection,
but also guarantees an accurate result.
1. General Blood Collection
2. Venipuncture Equipment
General Blood collection equipment and supplies
 Blood - Drawing Station
 Phlebotomy Chair
 Equipment carrier
 A new pair of gloves and glove liners
 Antiseptics
 Disinfectants
Continuation:
 Hand sanitizer& wall-mounted hand sanitizer dispenser
 Gauze Pads
 Bandages
 1x3 inches glass microscope slides
 Pen with non smear ink
 Watch with timer
 Needle and sharps disposal containers
 Biohazard bags
Venipuncture equipment
1. Vein-Locating Devices
2. Tourniquet
3. Needles
4. Needle gauge
5. Evacuated Tube System (ETS)
ANTISEPTICS AND DISINFECTANTS USED IN
VENIPUNCTURE
Disinfectants
 EPA -registered sodium hypochlorite
 1:100 dilution and 1-10 dilution for spills prior to
clean-up procedures
 contact time required is 10 minutes.
Antiseptics
 70% ethyl alcohol,
 70% isopropyl alcohol,
 Benzalkonium chloride,
 chlorhexidine gluconate,
 hydrogen peroxide,
 povidine-iodine and tincture iodine.
PHLEBOTOMY NEEDLES
Types of phlebotomy needles that are used in evacuated
tube systems or syringe systems:
1. multi-sample needles
2. hypodermic needles
3. winged infusion
Figure 4.4 The different types of phlebotomy needles
Courtesy of Quad IT Solutions, Inc. (Modified from Phlebotomy Essential,
5th Edition)
EVACUATED TUBE SYSTEM (ETS) AND SYRINGE
SYSTEM COMPONENTS
Syringe System
• used for patients as an alternative method when
drawing blood from patients with small or difficult
veins.
• traditional collection procedure that uses a syringe to
draw blood from the patient and the collected
specimen is transferred into tubes.
Continuation:
• The components of this system include:
(1) syringe needle that has a resheating feature, and
(2) a syringe which is divided into graduated barrel and
plunger.
Figure 4.5 Components of the Syringe System
Evacuated Tube System
• closed collection system that is considered the most
efficient system for collecting blood samples.
• allows numerous tubes to be collected in a single
venipuncture.
• composed of multi-sample needles, tube holders and
evacuated tubes which prevent the exposure of the
specimen from contaminants.
• evacuated tubes are color coded tubes filled with
predetermined volume of blood by vacuum.
Figure 4.6 Components of an Evacuated Tube System (ETS)
CATEGORIES OF ADDITIVES USED IN BLOOD
COLLECTION 
 anticoagulants
 special-use anticoagulants
 antiglycolytic agents
 clot aviators
 thrixotropic gel separator
 trace element-free tubes
COLOR CODING IN IDENTIFYING ADDITIVES IN
BLOOD COLLECTION TUBES
  An additive is any substance that is placed within the
tube and they can be used in ETS tubes to prevent clotting
and preserving the components.
Table 4.1 Color Coding for tube caps

Cap Color Specification

Red No additive

Orange Coagulant

Yellow Coagulant and Separation Gel

Green Herapin Sodium

Light Green Herapin Lithium

Purple EDTA Coagulant

Light Blue Sodium Citrate 1:9

Black Sodium Citrate 1:4

Grey Potassium Oxalate Monohydrate and Sodium Flouride


ORDER OF DRAW refers to the specific order that must be followed
by a phlebotomist when collecting blood samples to prevent any cross-
contamination especially when dealing with multiple collection tubes.
Not following the prescribed sequence affects the test results due to
cross contamination.
 Table 4.2 Order of draw and stopper color

Order of Draw Tube stopper color

Blood Culture
Yellow SPS
(sterile collections)
Coagulation tube Light Blue

Glass non-additive tube Red

Plastic clot activator tube Red

Serum separator tube (PSTs) Red and grey rubber, Gold plastic
Green and grey rubber, Light green
Herapin tube
plastic
EDTA tube Lavender, pink or purple
Plasma-preparation
Pearl top
tube (PPTs)
Oxalate/fluoride tubes Gray

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