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Bisubstrate Enzyme Catalysed Reaction

The document discusses bisubstrate enzyme catalyzed reactions, which make up 60% of known biochemical reactions. It describes substrates as A, B, C, D and products as P, Q, R, S. Enzymes are designated as E, F, G. Reactions are classified based on the number of substrates and products, such as uni-ter (one substrate, three products) or bi-bi (two substrates, two products). Ordered and random bi-bi reactions as well as ping pong mechanisms are described.

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santhosh n
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50 views

Bisubstrate Enzyme Catalysed Reaction

The document discusses bisubstrate enzyme catalyzed reactions, which make up 60% of known biochemical reactions. It describes substrates as A, B, C, D and products as P, Q, R, S. Enzymes are designated as E, F, G. Reactions are classified based on the number of substrates and products, such as uni-ter (one substrate, three products) or bi-bi (two substrates, two products). Ordered and random bi-bi reactions as well as ping pong mechanisms are described.

Uploaded by

santhosh n
Copyright
© © All Rights Reserved
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd
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Bisubstrate enzyme catalysed reaction

60% of known biochemical reactions are bisubstrate reactions

• Transferase reactions

• oxidation-reduction reactions
W.W. Cleland
• Substrates designated A, B, C, and D.
• Products designated P, Q, R, and S.
• Stable enzyme designated E, F, and G
*E being the free enzyme
The numbers of reactants and products in a given reaction are
specified, in order, by the terms Uni (one), Bi (two), Ter (three), and
Quad (four).

Reaction requiring one substrate and yielding three products is


designated a Uni Ter reaction.

Reaction requiring two substrate and yielding two products is


designated a Bi Bi reaction.
Sequential Reactions
Ordered Bi Bi reaction

Many NAD+ and NADP+ requiring dehydrogenases follow an Ordered Bi Bi


mechanism in which the coenzyme is the leading reactant.
Random Bi Bi Reaction

Some dehydrogenases and kinases operate through Random Bi Bi


mechanisms.
Ping Pong Reactions
Mechanisms in which one or more products are released before all
substrates have been added are known as Ping Pong reactions.

Chymotrypsin ,transaminase, and some flavoenzymes, react with Ping


Pong mechanisms
lock-and-key hypothesis:
Emil Fischer’s discover, that glycolytic
enzymes can distinguish between
stereoisomeric sugars. He made the
hypothesis like;
“specificity of an enzyme (the lock) for its
substrate (the key) arise from their
geometrically complementary shapes”
Induced-fit hypothesis

“Structure of a substrate may be


complementary to that of the active site
in the enzyme-substrate complex, but not
in the free enzyme: a conformational
change takes place in the enzyme during
the binding of substrate which results in
the required matching of structures”
• Necessity for the classification of enzyme
• Necessity for the classification of enzyme
• Necessity for the classification of enzyme
• Necessity for the classification of enzyme
• Necessity for the classification of enzyme
• Necessity for the classification of enzyme
• Necessity for the classification of enzyme
• Necessity for the classification of enzyme
• Necessity for the classification of enzyme
• Necessity for the classification of enzyme
• Necessity for the classification of enzyme
• Necessity for the classification of enzyme
• Necessity for the classification of enzyme
• Necessity for the classification of enzyme
• Necessity for the classification of enzyme
• Necessity for the classification of enzyme

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