Chapter 38

Angiosperm Reproduction
and Biotechnology

PowerPoint® Lecture Presentations for

Biology
Eighth Edition
Neil Campbell and Jane Reece

Lectures by Chris Romero, updated by Erin Barley with contributions from Joan Sharp
Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings
Overview: Flowers of Deceit

• Angiosperm flowers can attract pollinators

using visual cues and volatile chemicals
• Many angiosperms reproduce sexually and
asexually
• Symbiotic relationships are common between
plants and other species
• Since the beginning of agriculture, plant
breeders have genetically manipulated traits of
wild angiosperm species by artificial selection
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Fig. 38-1
Concept 38.1: Flowers, double fertilization, and fruits
are unique features of the angiosperm life cycle
• Diploid (2n) sporophytes produce spores by
meiosis; these grow into haploid (n)
gametophytes
• Gametophytes produce haploid (n) gametes by
mitosis; fertilization of gametes produces a
sporophyte

Video: Flower Blooming (time lapse)

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• In angiosperms, the sporophyte is the
dominant generation, the large plant that we
see
• The gametophytes are reduced in size and
depend on the sporophyte for nutrients
• The angiosperm life cycle is characterized by
“three Fs”: flowers, double fertilization, and
fruits

Video: Flower Plant Life Cycle (time lapse)

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Fig. 38-2

Anther Germinated pollen grain (n)

Anther Stigma (male gametophyte)
Stamen Carpel
Style
Filament Pollen tube Ovary
Ovary
Ovule
Embryo sac (n)
(female gametophyte)

Sepal FERTILIZATION
Petal
Egg (n)
Sperm (n)
Receptacle
Zygote
Mature sporophyte
(2n)
(a) Structure of an idealized flower Key plant (2n)

Haploid (n) Seed

Germinating
Diploid (2n) seed
Seed
Embryo (2n)
(sporophyte)

(b) Simplified angiosperm life cycle Simple fruit

Fig. 38-2a

Anther Stigma Carpel

Stamen
Style
Filament Ovary

Sepal
Petal

Receptacle

(a) Structure of an idealized flower

Fig. 38-2b

Anther Germinated pollen grain (n)

(male gametophyte)

Pollen tube Ovary

Ovule
Embryo sac (n)
(female gametophyte)

FERTILIZATION

Egg (n)
Sperm (n)
Zygote
Mature sporophyte
(2n)
Key plant (2n)

Haploid (n) Seed

Germinating
Diploid (2n) seed
Seed
Embryo (2n)
(sporophyte)

(b) Simplified angiosperm life cycle Simple fruit

Flower Structure and Function

• Flowers are the reproductive shoots of the

angiosperm sporophyte; they attach to a part of
the stem called the receptacle
• Flowers consist of four floral organs: sepals,
petals, stamens, and carpels

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• A stamen consists of a filament topped by an
anther with pollen sacs that produce pollen
• A carpel has a long style with a stigma on
which pollen may land
• At the base of the style is an ovary containing
one or more ovules
• A single carpel or group of fused carpels is
called a pistil

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• Complete flowers contain all four floral organs

• Incomplete flowers lack one or more floral

organs, for example stamens or carpels
• Clusters of flowers are called inflorescences

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Development of Male Gametophytes in Pollen
Grains
• Pollen develops from microspores within
the microsporangia, or pollen sacs, of anthers
• If pollination succeeds, a pollen grain
produces a pollen tube that grows down into
the ovary and discharges sperm near the
embryo sac
• The pollen grain consists of the two-celled
male gametophyte and the spore wall

Video: Bee Pollinating Video: Bat Pollinating Agave Plant

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Fig. 38-3
(a) Development of a male (b) Development of a female
gametophyte (in pollen grain) gametophyte (embryo sac)

Microsporangium
(pollen sac)
Megasporangium (2n)

Microsporocyte (2n) Ovule Megasporocyte (2n)

MEIOSIS Integuments (2n)

Micropyle
4 microspores (n)

Surviving
megaspore (n)
Each of 4
microspores (n) MITOSIS

Female gametophyte
Ovule
3 antipodal cells (n)

(embryo sac)
Generative cell (n) Male
gametophyte
2 polar nuclei (n)
1 egg (n)
Nucleus of Integuments (2n)
tube cell (n) 2 synergids (n)
20 µm

Ragweed
pollen Embryo
100 µm

75 µm grain sac
Fig. 38-3a
(a) Development of a male
gametophyte (in pollen grain)

Microsporangium
(pollen sac)

Microsporocyte (2n)

MEIOSIS

4 microspores (n)

Each of 4
microspores (n)
MITOSIS
Generative cell (n) Male
gametophyte

Nucleus of
tube cell (n)
20 µm

Ragweed
pollen
75 µm grain
Development of Female Gametophytes (Embryo
Sacs)
• Within an ovule, megaspores are produced by
meiosis and develop into embryo sacs, the
female gametophytes

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Fig. 38-3b
(b) Development of a female
gametophyte (embryo sac)

Megasporangium (2n)

Ovule Megasporocyte (2n)

MEIOSIS Integuments (2n)

Micropyle

Surviving
megaspore (n)

MITOSIS

Female gametophyte
Ovule
3 antipodal cells (n)

(embryo sac)
2 polar nuclei (n)
1 egg (n)
Integuments (2n)
2 synergids (n)

Embryo
100 µm

sac
Pollination

• In angiosperms, pollination is the transfer of

pollen from an anther to a stigma
• Pollination can be by wind, water, bee, moth
and butterfly, fly, bird, bat, or water

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Fig. 38-4a
Abiotic Pollination by Wind

Hazel staminate flowers

(stamens only)

Hazel carpellate flower

(carpels only)
Fig. 38-4b

Pollination by Bees

Common dandelion under

normal light

Common dandelion under

ultraviolet light
Fig. 38-4c
Pollination by Moths and Butterflies

Anther

Stigma

Moth on yucca flower

Fig. 38-4d

Pollination by Flies

Fly egg
Blowfly on carrion flower
Fig. 38-4e

Pollination by Birds

Hummingbird drinking nectar of poro flower

Fig. 38-4f

Pollination by Bats

Long-nosed bat feeding on cactus flower at night

Double Fertilization

• After landing on a receptive stigma, a pollen

grain produces a pollen tube that extends
between the cells of the style toward the ovary
• Double fertilization results from the discharge
of two sperm from the pollen tube into the
embryo sac
• One sperm fertilizes the egg, and the other
combines with the polar nuclei, giving rise to
the triploid (3n) food-storing endosperm
Animation: Plant Fertilization

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Fig. 38-5
Stigma Pollen grain

Pollen tube
2 sperm

Style
Ovary
Polar nuclei
Ovule

Micropyle Egg

Ovule
Polar nuclei
Egg
Synergid
2 sperm

Endosperm
nucleus (3n)
(2 polar nuclei
plus sperm)

Zygote (2n)
(egg plus sperm)
Fig. 38-5a

Stigma Pollen grain

Pollen tube

2 sperm

Style

Ovary

Ovule Polar nuclei

Micropyle Egg
Fig. 38-5b

Ovule

Polar nuclei

Egg

Synergid

2 sperm
Fig. 38-5c

Endosperm
nucleus (3n)
(2 polar nuclei
plus sperm)

Zygote (2n)
(egg plus sperm)
Fig. 38-6

EXPERIMENT

Wild-type Arabidopsis pop2 mutant Arabidopsis

Micropyle Ovule Ovule

20 µm

Seed stalk Pollen tube Many pollen Seed stalk

growing toward tubes outside
micropyle seed stalk
Seed Development, Form, and Function

• After double fertilization, each ovule develops

into a seed
• The ovary develops into a fruit enclosing the
seed(s)

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Endosperm Development

• Endosperm development usually precedes

embryo development
• In most monocots and some eudicots,
endosperm stores nutrients that can be used
by the seedling
• In other eudicots, the food reserves of the
endosperm are exported to the cotyledons

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Embryo Development

• The first mitotic division of the zygote is

transverse, splitting the fertilized egg into a
basal cell and a terminal cell

Animation: Seed Development

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Fig. 38-7

Ovule
Endosperm
nucleus
Integuments
Zygote

Zygote
Terminal cell
Basal cell
Proembryo
Suspensor

Basal cell

Cotyledons
Shoot
apex
Root
apex Seed coat
Suspensor Endosperm
Structure of the Mature Seed

• The embryo and its food supply are enclosed

by a hard, protective seed coat
• The seed enters a state of dormancy

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• In some eudicots, such as the common garden
bean, the embryo consists of the embryonic
axis attached to two thick cotyledons (seed
leaves)
• Below the cotyledons the embryonic axis is
called the hypocotyl and terminates in the
radicle (embryonic root); above the cotyledons
it is called the epicotyl

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Fig. 38-8
Seed coat Epicotyl

Hypocotyl

Radicle
Cotyledons

(a) Common garden bean, a eudicot with thick cotyledons

Seed coat
Endosperm
Cotyledons
Epicotyl
Hypocotyl
Radicle

(b) Castor bean, a eudicot with thin cotyledons

Scutellum Pericarp fused

(cotyledon) with seed coat

Endosperm
Coleoptile
Epicotyl
Hypocotyl
Coleorhiza
Radicle

(c) Maize, a monocot

Fig. 38-8a

Seed coat Epicotyl

Hypocotyl

Radicle
Cotyledons

(a) Common garden bean, a eudicot with thick cotyledons

• The seeds of some eudicots, such as castor
beans, have thin cotyledons

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Fig. 38-8b

Seed coat
Endosperm
Cotyledons
Epicotyl
Hypocotyl
Radicle

(b) Castor bean, a eudicot with thin cotyledons

• A monocot embryo has one cotyledon

• Grasses, such as maize and wheat, have a

special cotyledon called a scutellum
• Two sheathes enclose the embryo of a grass
seed: a coleoptile covering the young shoot
and a coleorhiza covering the young root

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Fig. 38-8c

Scutellum Pericarp fused

(cotyledon) with seed coat

Endosperm
Coleoptile
Epicotyl
Hypocotyl
Coleorhiza
Radicle

(c) Maize, a monocot

Seed Dormancy: An Adaptation for Tough Times

• Seed dormancy increases the chances that

germination will occur at a time and place most
advantageous to the seedling
• The breaking of seed dormancy often requires
environmental cues, such as temperature or
lighting changes

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Seed Germination and Seedling Development

• Germination depends on imbibition, the

uptake of water due to low water potential of
the dry seed
• The radicle (embryonic root) emerges first

• Next, the shoot tip breaks through the soil

surface

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• In many eudicots, a hook forms in the
hypocotyl, and growth pushes the hook above
ground
• The hook straightens and pulls the cotyledons
and shoot tip up

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Fig. 38-9
Foliage leaves
Cotyledon
Epicotyl
Hypocotyl

Cotyledon Cotyledon
Hypocotyl

Hypocotyl

Radicle
Seed coat

(a) Common garden bean

Foliage leaves

Coleoptile
Coleoptile

Radicle

(b) Maize
Fig. 38-9a

Foliage leaves
Cotyledon
Epicotyl
Hypocotyl

Cotyledon Cotyledon
Hypocotyl

Hypocotyl

Radicle
Seed coat

(a) Common garden bean

• In maize and other grasses, which are
monocots, the coleoptile pushes up through the
soil

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Fig. 38-9b

Foliage leaves

Coleoptile
Coleoptile

Radicle

(b) Maize
Fruit Form and Function

• A fruit develops from the ovary

• It protects the enclosed seeds and aids in seed

dispersal by wind or animals
• A fruit may be classified as dry, if the ovary
dries out at maturity, or fleshy, if the ovary
becomes thick, soft, and sweet at maturity

Animation: Fruit Development

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• Fruits are also classified by their development:
– Simple, a single or several fused carpels

– Aggregate, a single flower with multiple

separate carpels
– Multiple, a group of flowers called an
inflorescence

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Fig. 38-10

Carpels Stigma Style

Stamen Flower Petal
Ovary
Stamen

Stamen
Sepal
Stigma Ovary
Ovule (in receptacle)
Ovule
Pea flower Raspberry flower Pineapple inflorescence Apple flower
Each segment Remains of
Carpel develops stamens and styles
(fruitlet) from the
Stigma Sepals
Seed carpel
Ovary of one
flower

Stamen

Seed
Receptacle
Pea fruit Raspberry fruit Pineapple fruit Apple fruit
(a) Simple fruit (b) Aggregate fruit (c) Multiple fruit (d) Accessory fruit
Fig. 38-10a

Ovary
Stamen

Stigma

Ovule
Pea flower

Seed

Pea fruit
(a) Simple fruit
Fig. 38-10b
Carpels Stamen

Raspberry flower

Carpel
(fruitlet)
Stigma

Ovary

Stamen

Raspberry fruit
(b) Aggregate fruit
Fig. 38-10c
Flower

Pineapple inflorescence
Each segment
develops
from the
carpel
of one
flower

Pineapple fruit
(c) Multiple fruit
• An accessory fruit contains other floral parts
in addition to ovaries

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Fig. 38-10d
Stigma Style
Petal

Stamen
Sepal
Ovary
Ovule (in receptacle)

Apple flower
Remains of
stamens and styles
Sepals

Seed
Receptacle
Apple fruit
(d) Accessory fruit
• Fruit dispersal mechanisms include:
– Water

– Wind

– Animals

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Fig. 38-11a
Dispersal by Water

Coconut
Fig. 38-11b

Dispersal by Wind

Winged seed Dandelion “parachute”

of Asian
climbing gourd

Winged fruit of maple

Tumbleweed
Fig. 38-11c

Dispersal by Animals

Barbed fruit
Seeds in feces

Seeds carried to Seeds buried in caches

ant nest
Concept 38.2: Plants reproduce sexually,
asexually, or both
• Many angiosperm species reproduce both
asexually and sexually
• Sexual reproduction results in offspring that are
genetically different from their parents
• Asexual reproduction results in a clone of
genetically identical organisms

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Mechanisms of Asexual Reproduction

• Fragmentation, separation of a parent plant

into parts that develop into whole plants, is a
very common type of asexual reproduction
• In some species, a parent plant’s root system
gives rise to adventitious shoots that become
separate shoot systems

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Fig. 38-12
• Apomixis is the asexual production of seeds
from a diploid cell

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Advantages and Disadvantages of Asexual Versus
Sexual Reproduction
• Asexual reproduction is also called vegetative
reproduction
• Asexual reproduction can be beneficial to a
successful plant in a stable environment
• However, a clone of plants is vulnerable to
local extinction if there is an environmental
change

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• Sexual reproduction generates genetic
variation that makes evolutionary adaptation
possible
• However, only a fraction of seedlings survive

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Mechanisms That Prevent Self-Fertilization

• Many angiosperms have mechanisms that

make it difficult or impossible for a flower to
self-fertilize
• Dioecious species have staminate and
carpellate flowers on separate plants

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Fig. 38-13

(a) Sagittaria latifolia staminate flower (left) and carpellate

flower (right)

Stamens Styles
Styles Stamens

Thrum flower Pin flower

(b) Oxalis alpina flowers
Fig. 38-13a

(a) Sagittaria latifolia staminate flower (left) and carpellate

flower (right)
• Others have stamens and carpels that mature
at different times or are arranged to prevent
selfing

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Fig. 38-13b

Stamens Styles
Styles Stamens

Thrum flower Pin flower

(b) Oxalis alpina flowers
• The most common is self-incompatibility, a
plant’s ability to reject its own pollen
• Researchers are unraveling the molecular
mechanisms involved in self-incompatibility
• Some plants reject pollen that has an S-gene
matching an allele in the stigma cells
• Recognition of self pollen triggers a signal
transduction pathway leading to a block in
growth of a pollen tube
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Vegetative Propagation and Agriculture

• Humans have devised methods for asexual

propagation of angiosperms
• Most methods are based on the ability of plants
to form adventitious roots or shoots

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Clones from Cuttings

• Many kinds of plants are asexually reproduced

from plant fragments called cuttings
• A callus is a mass of dividing undifferentiated
cells that forms where a stem is cut and
produces adventitious roots

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Grafting

• A twig or bud can be grafted onto a plant of a

closely related species or variety
• The stock provides the root system

• The scion is grafted onto the stock

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Test-Tube Cloning and Related Techniques

• Plant biologists have adopted in vitro methods

to create and clone novel plant varieties
• Transgenic plants are genetically modified
(GM) to express a gene from another organism

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Fig. 38-14

(a) Undifferentiated carrot cells (b) Differentiation into plant

• Protoplast fusion is used to create hybrid
plants by fusing protoplasts, plant cells with
their cell walls removed

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Fig. 38-15

50 µm
Concept 38.3: Humans modify crops by breeding
and genetic engineering
• Humans have intervened in the reproduction
and genetic makeup of plants for thousands of
years
• Hybridization is common in nature and has
been used by breeders to introduce new genes
• Maize, a product of artificial selection, is a
staple in many developing countries

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Fig. 38-16
Plant Breeding

• Mutations can arise spontaneously or can be

induced by breeders
• Plants with beneficial mutations are used in
breeding experiments
• Desirable traits can be introduced from
different species or genera
• The grain triticale is derived from a successful
cross between wheat and rye

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Plant Biotechnology and Genetic Engineering

• Plant biotechnology has two meanings:

– In a general sense, it refers to innovations in
the use of plants to make useful products
– In a specific sense, it refers to use of GM
organisms in agriculture and industry

• Modern plant biotechnology is not limited to

transfer of genes between closely related
species or varieties of the same species

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Reducing World Hunger and Malnutrition

• Genetically modified plants may increase the

quality and quantity of food worldwide
• Transgenic crops have been developed that:
– Produce proteins to defend them against
insect pests
– Tolerate herbicides

– Resist specific diseases

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Fig. 38-17
• Nutritional quality of plants is being improved

• “Golden Rice” is a transgenic variety being

developed to address vitamin A deficiencies
among the world’s poor

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Fig. 38-18

Genetically modified rice

Ordinary rice
Reducing Fossil Fuel Dependency

• Biofuels are made by the fermentation and

distillation of plant materials such as cellulose
• Biofuels can be produced by rapidly growing
crops

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The Debate over Plant Biotechnology

• Some biologists are concerned about risks of

releasing GM organisms into the environment

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Issues of Human Health

• One concern is that genetic engineering may

transfer allergens from a gene source to a plant
used for food

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Possible Effects on Nontarget Organisms

• Many ecologists are concerned that the

growing of GM crops might have unforeseen
effects on nontarget organisms

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Addressing the Problem of Transgene Escape

• Perhaps the most serious concern is the

possibility of introduced genes escaping into
related weeds through crop-to-weed
hybridization

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• Efforts are underway to prevent this by
introducing:
– Male sterility

– Apomixis

– Transgenes into chloroplast DNA (not

transferred by pollen)
– Strict self-pollination

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Fig. 38-UN1

Endosperm
nucleus (3n)
(2 polar nuclei
plus sperm)

Zygote (2n)
(egg plus sperm)
Fig. 38-UN2
You should now be able to:

1. Describe how the plant life cycle is modified in

angiosperms

2. Identify and describe the function of a sepal,

petal, stamen (filament and anther), carpel
(style, ovary, ovule, and stigma), seed coat,
hypocotyl, radicle, epicotyl, endosperm,
cotyledon

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You should now be able to:

3. Distinguish between complete and incomplete

flowers; bisexual and unisexual flowers;
microspores and megaspores; simple,
aggregate, multiple, and accessory fruit

4. Describe the process of double fertilization

5. Describe the fate and function of the ovule,

ovary, and endosperm after fertilization

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6. Explain the advantages and disadvantages of
reproducing sexually and asexually

7. Name and describe several natural and

artificial mechanisms of asexual reproduction

8. Discuss the risks of transgenic crops and

describe four strategies that may prevent
transgene escape

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