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From the Virtual Microbiology Classroom on ScienceProfOnline.com Image: Compound microscope objectives, T. Port
 Hi! I’m E. coli 

Introduction
to
Microbiology

Image: Ermahgerd Microbiology!, variation by T. Port.

From the Virtual Microbiology Classroom on ScienceProfOnline.com Click here for info on the history of the Ermahgerd meme.
 Lets start the
semester by
learning how to pr
operly cough and s
neeze
!

DVD 665

From the Virtual Microbiology Classroom on ScienceProfOnline.com

 Microbiology

 Check your course # (CRN) to make sure that you

are in the current course.

 Sign in every time you attend class.

Tami Port…Who’s she?

Microbiology…What is it?

From the Virtual Microbiology Classroom on ScienceProfOnline.com Image: Chlamydia, Giant Microbes
 This should not
be your first college
biology course.

Nearly every Health Science curriculum at this institution that

requires Microbiology, either has:
1. Prerequisite of Cell Biology or

2. 1 year of HS bio, chem & algebra with grade => 2.0. or

3. Completed college level intro biology, chem and algebra with grades => 2.0.

What I might spend 2 slides on, an intro bio course might spend
2-weeks on. See the potential problem here?

From the Virtual Microbiology Classroom on ScienceProfOnline.com

 Courtesy
yawn

 Be Respectful

 Be On Time
(I reserve the right to close and lock
the door 10 min after class or lab
begins.)

 Pay Attention

 If you can’t pay attention,

at least keep it to yourself
… don’t disrupt others.

 Cell Phones off/silenced

From the Virtual Microbiology Classroom on ScienceProfOnline.com

 Microbes & You

• Normal Flora
– Q: Did you always have them?
– Q: Are they everywhere on your body?
– Q: Are normal flora ever harmful?

• Opportunistic Pathogens (part-time bad guys)

• Pathogens (full-time bad guys)

Image and great micro info in

Todar’s Textbook of Bacteriology. From the Virtual Microbiology Classroom on ScienceProfOnline.com
 Types of symbiotic
microbe-host relationships
Mutualism * Commensalism * Parasitism
What are the benefits of normal flora?

Benefit to the bacteria = They have a place to eat,

survive and multiply.
Q: What is the “human
  microbiome”?
Benefits to the human =
• Bacteria may produce vitamins (such as B and K), and
break down food that host cant normally digest.

• Normal flora protect host against infection by

pathogenic organisms:
- take up space, so pathogen has nowhere to set up shop
- may out-compete the invader for available nutrients
- may produce anti-bacterial chemicals (bacteriocins)
- long-term relationship with the human immune system

Image: Arm plate of normal flora, T. Port. From the Virtual Microbiology Classroom on ScienceProfOnline.com
 Types of symbiotic
microbe-host relationships
Mutualism * Commensalism * Parasitism

- One partner in the relationship – Pathogens that harm their

benefits. The other neither host.
benefits nor is harmed. – Cost to the host can vary
from slight to fatal.
– External parasites
Streptococcus (ectoparasite) cause
pyogenes, a infestation.
pathogen that can – Internal parasites
cause Strep throat,
post-partum fever, (endoparasite) cause
pneumonia and infection.
necrotizing fasciitis.

Image: Blood Agar showing Beta hemolysis from pathogen

Streptococcus pyogenes, T. Port. From the Virtual Microbiology Classroom on ScienceProfOnline.com
 Q: Why are you in
this class?

“Because it is a requirement to get into my

program of study.”

Why else?

From the Virtual Microbiology Classroom on ScienceProfOnline.com

 Impact of Infectious Disease

Average life span:

- Bronze age 26 yrs
- Medieval Europe (400 – 1500 ad) 30 yrs
- Early 20th century 50 – 64 yrs
- Now world average 71 years (USA., 78.7)

Q: Why?

From the Virtual Microbiology Classroom on ScienceProfOnline.com

 Impact of Infectious Disease

Bubonic Plague
• a.k.a. Black Plague & Black
Death

• Caused by bacteria Yersenia

pestis.
• Several pandemics of plague
have occurred throughout
history.
• 50 million deaths between years
Doctor beak from Roman
engraving, 1656 Physician 1346 – 50.
attire for protection from
the Bubonic plague (a.k.a • Nearly 1/2 of Europe perished
Black death).
in this plague

Images: Yersenia pestis, CDC; Black Death illustration,

Toggenburg Bible (1411); Black Plague Physician Attire,
From the Virtual Microbiology Classroom on ScienceProfOnline.com History of Medicine, Paul Furst
 Impact of
Infectious Disease

• The Sedlec Ossuary, small Roman

Catholic chapel, located in the Czech
Republic.

• Contains ~ 40,000-70,000 human

skeletons which have been artistically
arranged to form decorations and
furnishings for the chapel.
• Many of these bones were from
bubonic plague victims.

• “Bring out your dead!” plague scene

from Monty Python & Holy Grail.

Images: From the Dance of Death by Michael Wolgemut

From the Virtual Microbiology Classroom on ScienceProfOnline.com (1493) Sedlec Ossuary, Czech Republic by Marcin Szala)
 Impact of
Infectious Disease

Bubonic Plague

Images: Worldwide distribution of plague 1998, CDC;

Waste in open market, frabood; Brown rat, National
Park Service; Scanning electron micrograph of flea,
From the Virtual Microbiology Classroom on ScienceProfOnline.com
CDC; Yersenia pestis, CDC
 Impact of Infectious Disease
Smallpox
 Caused two airborne virus variants, Variola major
and Variola minor.

 Deadly disease that, in survivors, can cause

disfigurement and blindness.

 Killed Queen Mary II of England, Emperor Joseph I

of Austria, King Luis I of Spain, Tsar Peter II and
King Louis XV of France.

 Approx 300 million deaths worldwide just in the

20th century.

 Eradicated in 1980 though widespread vaccination.

 Now still possible weapon of bioterrorism.

Images: Girl with smallpox, James Hicks, CDC;

 Watch
Electron micrograph thisvirus,
of smallpox short National
Magnus Manske; From the Virtual Microbiology Classroom on ScienceProfOnline.com
 Impact of Infectious Disease

Influenza
 Infectious disease caused by RNA
viruses in the family Orthomyxoviridae.

 Spanish flu pandemic 1918; more than 50

million deaths.

 In the U.S. seasonal flu kills thousands

of people every year (mainly very young and old).

 Q: How is pandemic influenza different

from seasonal flu?

 Watch this short National Geographic

video “How Flu Viruses Attack”.
Images: Influenza virus, Cynthia Goldsmith; Walter Reed
Hospital Spanish Flu Ward, by Harris & Ewing via Library of
Congress; Symptoms of influenza, Mikael Häggström From the Virtual Microbiology Classroom on ScienceProfOnline.com
 Impact of Infectious Disease

AIDS
- Acquired immune deficiency syndrome
(AIDS).

- Caused by HIV (human immunodeficiency) virus, a

retrovirus that infects T-cells of the
immune system.
- AIDS fatalities typically die of
opportunistic infections and tumors.
Everything comes
- More than 39 million people have died from
from HIV since it was recognized in 1981. somewhere…
Where did HIV
- With anti-retroviral drug therapy, more, come from?
and more people are living with aids. This semester
we will listen to
- New breakthroughs in anti-retroviral the
drugs can reduce contagiousness and RADIOLAB
transmission of HIV. podcast
“Patient 0” to
- Recent HIV News:“New find out.
Insights into HIV Vaccines Will Improve Drug

Development”, Science News 2013

Images: Aids viruses budding off a lymphocyte, C.
From the Virtual Microbiology Classroom on ScienceProfOnline.com Goldsmith, CDC; Main symptoms of Aids, Mikael Haggstrom
 Impact of Infectious Disease
HBV
 Hepatitis B = infectious
inflammatory illness of the liver
caused by hepatitis B virus (HBV).

 Virus transmitted by exposure to

infectious blood or body fluids.

 Risk of HBV transmission from

carrier 10 – 35%. Health care
workers high risk group.
 The hepatitis B virus is 50 to 100 times more infectious than HIV.
 HBV infection may be either acute (self-limiting) or chronic (long-standing). Persons
with self-limiting infection clear the infection spontaneously within weeks to
months.
 Watch this video on Hepatitis A & B and how they can damage the liver.
 Get vaccinated!

Images HBV virions, PHIL #5631; HBV prevalence 2005, From the Virtual Microbiology Classroom on ScienceProfOnline.com
 Impact of Infectious Disease

SARS

 Severe acute respiratory

syndrome caused by the SARS
coronavirus, an enveloped RNA virus.

 One near pandemic to date, with

8,096 known infected cases and 774
deaths (fatality rate of 9.6%).

 Within a matter of weeks in early

2003, SARS spread from a province
of China to infect individuals in 37
countries.

 Majority of those who became sick

were household contacts and health
care workers.
Images: Sars coronavirus, CDC From the Virtual Microbiology Classroom on ScienceProfOnline.com
 Where does the Ebola virus hide?
Disease Please: May be present in more animals than previously
Ebola Virus Disease thought, including chimpanzees, gorillas, fruit
bats, monkeys, antelopes, porcupines, rodents,
(a.k.a. Ebola Hemorrhagic Fever) dogs, pigs and humans.
Caused by ssRNA animal viruses, a filovirus

 Ebola viruses, and their relative Marbug viruses cause severe and
often fatal hemorrhagic fever in humans and other mammals.

 EVD first appeared in 1976 in 2 simultaneous African outbreaks, one

in Sudan, and the other in Democratic Republic of Congo.

 The 2014 Ebola epidemic is the largest in history, affecting multiple

countries in West Africa, causing more than 8,000 deaths.

Transmission
 Transmitted from wild animals and spreads in the human population
through human-to-human transmission.

 Spreads through direct contact with bodily fluids of infected

people and animals, as well as from contaminated surfaces.

 Health-care workers frequently infected when infection control

precautions not strictly practiced.

 Burial ceremonies where mourners have direct contact with body of

deceased Ebola victim can also play a role in transmission.

 People remain infectious as long as their body fluids contain the

virus. Recovered males can transmit through semen for up to 7
weeks after recovery.

Images: Ebola symptoms, Wiki. From the Virtual Microbiology Classroom on ScienceProfOnline.com
 What is a nosocomial infection?

There are many different types of 2011 HAI Prevalence Survey found
microbes that cause HAIs. Many are that on any given day, about 1 in 25
bacteria resistant to antibiotics. hospital patients has at least one
healthcare-associated infection.
A majority of HAIs include:
• Urinary tract infections There were an estimated 722,000
• Surgical site infections
HAIs in U.S acute care hospitals in
• Bloodstream infections
2011.
• Pneumonia
About 75,000 hospital patients
with HAIs died during their
hospitalizations.

Most of these
HAI infections are preventable.
.

Images: Surgeon PHIL #13551, S. aureus, PHIL #10046 From the Virtual Microbiology Classroom on ScienceProfOnline.com
 Q: What is the single most important
thing that you can do to prevent the
spread of infectious disease?

Find out by watching this

CDC (Centers for Disease Control)
video:
Put Your Hands Together
.

From the Virtual Microbiology Classroom on ScienceProfOnline.com

 What does it take to be a
butt-kicking, badass infectious disease?

One of the
best ways to
begin learning
about
infectious
disease is to
see the world
from the
perspective of
Plague Inc. is a FREE game
a pathogen!
app where you get to be an
infectious disease and figure
out what it takes to succeed.
 Confused?
Here are links to fun resources that further explain
what we discussed in this lecture:

 Intro to Microbiology Main Page on the Virtual Microbiology Classroom of Science Prof Online.

 Play Pandemic 2 a video game of strategy, where you try to become a successful pandemic microbe
and infect the world. My 14-year old, daughter and I recommend this one to you.

 “Quarantine” a scary movie about a new infectious disease.

 “Contagion” great dramatic movie that realistically depicts what could happen in outbreak of a novel,
virulent infectious disease.

 Play Disease Defenders educational video game, Rice University.

 Normal Flora webpage, by Douglas F. Fix. Interactive page where you can select an area of the body
and learn which normal flora typically colonize that location.

 “Catch My Disease” song by Ben Lee.

 Bacterial Pathogen Pronunciation Station , a webpage with links to audio files containing the
pronunciation of the bacterial names, created by Neal R. Chamberlain, Ph.D.

 Giant Microbes, a company that sells adorable stuffed microbes.

From the Virtual Microbiology Classroom on ScienceProfOnline.com
 Are microbes intimidating you?

Do yourself a favor. Use the…

Virtual Microbiology
Classroom (VMC) !
The VMC is full of resources to help you succeed,
including:
• practice test questions
• review questions
• study guides and learning objectives

You can access the VMC by going to the Science Prof Online website
www.ScienceProfOnline.com

Images: HIV, Giant Microbes; Prokaryotic cell, Mariana Ruiz