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Common Cell Lines and Their Uses

Cell lines are permanently established cell cultures that proliferate indefinitely when provided with fresh medium and space. They are derived from a single cell and thus have a uniform genetic makeup. Commonly used cell lines include mouse fibroblasts, HeLa cells from cervical cancer tissue, HEK293 cells from human embryonic kidney cells, PC12 cells from rat adrenal gland tumor, and CHO cells for protein production and research. Cell lines are characterized by their species, cell type, origin, morphology, presence of viruses, and applications such as recombinant protein expression, cancer research, and studying neurodegenerative diseases. Sf9 insect cells derived from fall armyworm moths are often used in suspension culture for large-scale protein production.

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70 views

Common Cell Lines and Their Uses

Cell lines are permanently established cell cultures that proliferate indefinitely when provided with fresh medium and space. They are derived from a single cell and thus have a uniform genetic makeup. Commonly used cell lines include mouse fibroblasts, HeLa cells from cervical cancer tissue, HEK293 cells from human embryonic kidney cells, PC12 cells from rat adrenal gland tumor, and CHO cells for protein production and research. Cell lines are characterized by their species, cell type, origin, morphology, presence of viruses, and applications such as recombinant protein expression, cancer research, and studying neurodegenerative diseases. Sf9 insect cells derived from fall armyworm moths are often used in suspension culture for large-scale protein production.

Uploaded by

Ishita guleria
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© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PPTX, PDF, TXT or read online on Scribd
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What is cell line

• A cell line is a permanently established cell culture that will proliferate indefinitely
given appropriate fresh medium and space.
• A cell culture developed from a single cell and therefore consisting of cells with a
uniform genetic make-up.
Commonly used cell lines

• Species: Mus Musculus


• Cell type: fibroblasts
• Origin: established from disaggregated Swiss albino mouse embryos in 1962; cells are
used for transfection studies with DNA viruses and as assay system for transformation
studies; cells have a high sensitivity to contact inhibition
• Morphology: fibroblasts growing adherently as monolayer with contact inhibition.
• Viruses: ELISA: reverse transcriptase negative; PCR: SMRV – 8.
• Application: This cell line is highly sensitive to sarcoma virus focus formation and
leukemia virus propagation and has proven to be very useful in DNA transfection
studies.
Viruses means used to these cell lines were used to accurate identification of viruses through ELISA or PCR
• Species: Homo sapiens
• Cell type: Cervix carcinoma
• Origin: established from the epitheloid cervix carcinoma of a 31- year-old black
woman in 1951; later diagnosis changed to adenocarcinoma; first aneuploid,
continuously cultured human cell line
• Morphology: epithelial-like cells growing in monolayers.
• Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-
8 -, HIV -, HPV +, HTLV-I/II -, MLV -, SMRV – 10
• Applications: Polio eradication, Improved cell culture practices, Chromosome
counting, Genome mapping, Human papilloma virus (HPV) vaccines.
• Cell Line: 293
• Species: Homo sapiens
• Cell type: Embryonic Kidney
• Origin: established from a human primary embryonal kidney transformed by adenovirus type 5 (Ad 5);
classified as risk category 1 according to the German Central Commission for Biological Safety (ZKBS); cell
line also known as HEK-293 (human embryonic kidney- 293)
• Morphology: adherent fibroblastoid cells growing as monolayer;
• Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV -, HTLV-I/II -,
MLV -, SMRV – 12
• Applications: highly efficient at producing large amounts of recombinant proteins, 293 cells can be used for
both transient and stable expression of desired genes, HEK293 are highly amenable to transfection, Cancer
research
• cell Line: PC 12
• Species: rat (Rattus norvegicus)
• Cell type: adrenal pheochromocytoma
• Origin: established from a transplantable rat adrenalpheochromocytoma in 1976; cells were described to
synthesize catecholamines (dopamine, norepinephrine); in response to nerve growth factor (NGF) a
neuronal phenotype could be induced reversibly
• Morphology: small cells growing in clumps in suspension, adhering poorly to plastic;
• Viruses: ELISA: reverse transcriptase negative; PCR: SMRV – 14
• Applications:  PC12 cells is to study the neurotoxic activity of various substances, for example, by
assessment of the effect on cell survival, neurite outgrowth, DNA damage or protein expression levels.
However, this cell line is also widely used as a model for neurodegenerative diseas
• CHO cells are used in many biological, medical, and pharmaceutical research
applications, including recombinant protein production, and studies of the epidermal
growth factor receptor
• Cell line: BC 1
• Species: human (Homo sapiens)
• Cell type: B cell lymphoma
• Origin: established from the malignant effusion samples of a 46-year-old man with AIDS-related
primary effusion lymphoma (PEL, stage IE-B) at diagnosis in 1992; cells are described to be
positive for human herpesvirus type 8 (HHV-8) and Epstein-Barr virus (EBV)
• Morphology: round cells growing singly and in small clusters in suspension
• Viruses: PCR: EBV +, HBV -, HCV -, HHV-8 +, HIV -, HTLV-I/-II -, MLV – 18
• Application: used to examine the infection of primary B lymphocytes, allows in vitro culture of
KSHV viral genomes.
Sf9 insect epithelial cells
• Derived from the ovaries of the fall armyworm moth (Spodoptera frugiperda), these cells
are probably related to all insect cell lines in labs worldwide.
• Sf9 insect epithelial cells can be cultured as adherent or suspension cells. Most cell lines
are adherent cells, which grow only on the surfaces of culture vessels. This limits the
number of cells you can expect to obtain from each culture.
• Furthermore, and because of the high volume-to-cell number ratio, suspension cultures
allow much more effective use of medium than adherent cultures.
• Sf9/baculovirus systems are typically preferred for large-scale protein production,
including industrial manufacture of mammalian proteins, including the vaccine for cervical
cancer CERVARIX
Application of cell lines to produce various product

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