04 Lecture Animation
04 Lecture Animation
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SCHOOL YEAR 2020-2021
WELCOME,
class TO SCIENCE
WORLD
JOSHUA VIDAL
YOUR TEACHER
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SHARE
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POP QUIZ
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OBJECTIVES
The students will be able to:
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VIDEO
Instruction: Watch the video using the link
below. You are only given 6 minutes to watch
it, after that you will be answering questions
out from the video using your Kahoot.
https://youtu.be/4OpBylwH9DU
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Question
Instruction: Answer the following in your
chatbox. Write in complete sentence.
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Outline
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Cell Theory
● Detailed study of the cell began in the 1830s
● A unifying concept in biology
● Originated from the work of biologists Schleiden
and Schwann in 1838-9
● States that:
● All organisms are composed of cells
● German botanist Matthais Schleiden in 1838
● German zoologist Theodor Schwann in 1839
● All cells come only from preexisting cells
● German physician Rudolph Virchow in 1850’s
● Cells are the smallest structural and functional unit of
organisms
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Organisms and Cells
Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display.
a. c.
b. 50 m d. 140 m
protei
n chloroplas
t plant and mous ros
animal frog e e
amin cells egg
o
acid viru
s ostric
most human an h
ato bacteria egg t egg
m blue
electron huma whale
microscope n
light
microscope
human
eye
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Microscopy and Amoeba proteus
Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display.
85 µm 200 nm 500 µm
amoeba, light pseudopod segment, transmission amoeba, scanning electron
micrograph electron micrograph
micrograph
electron source electron
eye electron beam gun
ocular lens electron beam
light
rays
electromagnetic
condenser lens electromagnetic
condenserl
enses
specimen
electromagnetic
objective lens
objective lens scanning
specimen coil
condenser lens
final
electromagnetic
Condenser electron
projector lens
lens detector
secondary
observation screen electrons TV
or specimen Viewing
light photographic plate screen
source
a. Compound light b Transmission electron c. Scanning electron
microscope . microscope microscope
a: © Robert Brons/Biological Photo Service; b: © M. Schliwa/Visuals Unlimited; c: © Kessel/Shih/Peter Arnold, Inc.
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Pre-Assessment
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Question
1. The following are the microscopes being
A
used for micrograph.
A. Telescope, Micrograph
B. Compound light microscope, transmission
electron microscope, Scanning electron
microscope
C. Only Compound microscope and Scanning
Microscope
D. All of the above
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Question
2.Chloroplast and bacteria can be
seen in what type of microscope?
A. Electron microscope
B. Light microscope C
C. Electron and light microscope
D. telescope
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Question
3. Amino acid can be seen in what
type of microscope?
A.Light microscope
B.Electron microscope B
C.Electron and light microscope
D.Telescope
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Question
A
A.Rudolf Virchow
B.Matthais Schleiden
C.Zacharias Jansen
D.Theodor Schwann
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Question
A
A.1930s
B. 17th -century
C. 1883
D. 21st -century
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Asynchronous
https://youtu.be/8IlzKri08kk
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Announcements/ Reminder
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Cell Structure and Function
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Microscopy Today: Compound Light
Microscope
● Light passed through specimen
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Compound Light Microscope
Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display.
85 µm
amoeba, light
micrograph
eye
ocular lens
light
rays
objective lens
specimen
condenser lens
light
source
a. Compound light
microscope
© Robert Brons/Biological Photo Service
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Prokaryotic Cells
● Lack a membrane-bound nucleus
● Structurally smaller and simpler than eukaryotic
cells (which have a nucleus).
● Prokaryotic cells are placed in two taxonomic
domains:
● Bacteria
● Archaea
● Live in extreme habitats
● Domains are structurally similar but biochemically
different
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The Structure of Bacteria
● Extremely small - 1–1.5 μm wide and 2–6 μm long
● Occur in three basic shapes:
● Spherical coccus,
● Rod-shaped bacillus,
● Spiral spirillum (if rigid) or spirochete (if flexible).
● Cell Envelope includes:
● Plasma membrane - lipid bilayer with imbedded and peripheral
protein
● Form internal pouches (mesosomes)
● Cell wall - maintains the shape of the cell and is strengthened by
peptidoglycan
● Glycocalyx - layer of polysaccharides on the outside of the cell
wall
● Well organized and resistant to removal (capsule)
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The Structure of Bacteria
Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display.
spirillum
spirochete bacillus
coccus
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The Structure of Bacteria
Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display.
Ribosome: Fimbriae:
site of protein hairlike bristles that
synthesis allow adhesion to
the surfaces
Inclusion body:
Conjugation pilus:
stored nutrients
elongated, hollow
for
appendage used for
later use
Mesosome: DNA transfer to other
plasma membrane bacterial cells
that folds into the Nucleoid:
cytoplasm and location of the
increases surface area bacterial
chromosome
Plasma membrane:
sheath around
cytoplasm
that regulates entrance
and exit of molecules
Cell wall:
covering that supports,
shapes, and protects cell
Glycocalyx:
gel-like coating outside
cell wall; if compact,
called
a capsule; if diffuse, called
a slime layer
Flagellum:
rotating filament Escherichia coli
present
in some bacteria that
pushes the cell forward
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The Structure of Bacteria Cytoplasm &
Appendages
● Cytoplasm
● Semifluid solution
● Bounded by plasma membrane
● Contains water, inorganic and organic molecules, and enzymes.
● Nucleoid is a region that contains the single, circular DNA
molecule.
● Plasmids are small accessory (extrachromosomal) rings of DNA
● Appendages
● Flagella – Provide motility
● Fimbriae – small, bristle-like fibers that sprout from the cell
surface
● Sex pili – rigid tubular structures used to pass DNA from cell to
cell
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Eukaryotic Cells
● Domain Eukarya includes:
● Protists Ex. amoebas
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Hypothesized Origin of Eukaryotic Cells
Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display.
Original
prokaryotic cell
DN
A
aerobic
bacterium
mitochondrion
chloroplast
photosynthetic
bacterium
Animal cell
has mitochondria,
but not chloroplasts.
Plant cell
has both mitochondria
and chloroplasts.
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Eukaryotic Cells: Organelles
● Eukaryotic cells are compartmentalized
● They contain small structures called organelles
● Perform specific functions
● Isolates reactions from others
● Two classes of organelles:
● Endomembrane system:
● Organelles that communicate with one another
● Via membrane channels
● Via small vesicles
● Energy related organelles
● Mitochondria & chloroplasts
● Basically independent & self-sufficient
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Animal Cell Anatomy
Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display.
Plasma membrane:
outer surface that
regulates entrance and
exit of molecules
protein
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Plant Cell Anatomy
Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display.
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Nucleus
● Command center of cell, usually near center
● Separated from cytoplasm by nuclear envelope
● Consists of double layer of membrane
● Nuclear pores permit exchange between nucleoplasm
& cytoplasm
● Contains chromatin in semifluid nucleoplasm
● Chromatin contains DNA of genes, and proteins
● Condenses to form chromosomes
● Chromosomes are formed during cell division
● Dark nucleolus composed of rRNA
● Produces subunits of ribosomes
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Anatomy of the Nucleus
Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display.
nuclear
envelope
nucleolu
s
phospholipi
d
(Bottom): Courtesy Ron Milligan/Scripps Research Institute; (Top right): Courtesy E.G. Pollock
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Ribosomes
● Are the site of protein synthesis in the cell
● Composed of rRNA
● Consists of a large subunit and a small subunit
● Subunits made in nucleolus
● May be located:
● On the endoplasmic reticulum (thereby making it
“rough”), or
● Free in the cytoplasm, either singly or in groups, called
polyribosomes
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Nucleus, Ribosomes, & ER
Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display.
Cytoplas
Endoplasmic m
reticulum
(ER)
ER membrane
protei
n 4. An enzyme removes
the signal Lumen of
peptide. ER
5. Ribosomal subunits and
mRNA break
away. The enzyme
protein remains in
the ER
and folds into its receptor
mRNA
final shape.
SRP
signal
recognition
particle (SRP)
2. Signal recognition
3. SRP attaches to receptor particle
(purple); (SRP) binds
a channel opens; and to signal
the peptide.
polypeptide enters ER..
signal
ribosoma peptide
l nuclear
ribosom
subunits pore
e
mRNA
mRNA DNA
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Endomembrane System
● Series of intracellular membranes that
compartmentalize the cell
● Restrict enzymatic reactions to specific
compartments within cell
● Consists of:
● Nuclear envelope
● Membranes of endoplasmic reticulum
● Golgi apparatus
● Vesicles
● Several types
● Transport materials between organelles of system
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Endomembrane System:
The Endoplasmic Reticulum
● A system of membrane channels and saccules (flattened vesicles)
continuous with the outer membrane of the nuclear envelope
● Rough ER
● Studded with ribosomes on cytoplasmic side
● Protein anabolism
● Synthesizes proteins
● Modifies and processes proteins
● Adds sugar to protein
● Results in glycoproteins
● Smooth ER
● No ribosomes
● Synthesis of lipids
● Site of various synthetic processes, detoxification, and storage
● Forms transport vesicles
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Endoplasmic Reticulum
Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display.
ribosome nuclear
s envelope
rough
endoplasmi
c
reticulum
smooth
endoplasmi
c
reticulum
0.0 m
© R. Bolender & D. Fawcett/Visuals Unlimited 8
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Endomembrane System:
The Golgi Apparatus
● Golgi Apparatus
● Consists of 3-20 flattened, curved saccules
● Resembles stack of hollow pancakes
● Modifies proteins and lipids
● Receives vesicles from ER on cis (or inner face)
● Packages them in vesicles
● Prepares for “shipment” in v Packages them in
vesicles from trans (or outer face)
● Within cell
● Export from cell (secretion, exocytosis)
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Golgi Apparatus
Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display.
secretion
transport saccules
vesicle
transport
vesicle
trans face
cis face
Golgi apparatus
Nucleus
0.1 m
Courtesy Charles Flickinger, from Journal of Cell Biology 49: 221-226, 1971, Fig. 1 page 224
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Endomembrane System: Lysosomes
● Membrane-bound vesicles (not in plants)
● Produced by the Golgi apparatus
● Contain powerful digestive enzymes and are highly
acidic
● Digestion of large molecules
● Recycling of cellular resources
● Apoptosis (programmed cell death, like tadpole losing tail)
● Some genetic diseases
● Caused by defect in lysosomal enzyme
● Lysosomal storage diseases (Tay-Sachs)
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Lysosomes
Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display.
lysosome
a: Courtesy Daniel S. Friend; b: Courtesy Robert D. Terry/Univ. of San Diego School of Medicine
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Endomembrane System: Summary
● Proteins produced in rough ER and lipids from
smooth ER are carried in vesicles to the Golgi
apparatus.
● The Golgi apparatus modifies these products and
then sorts and packages them into vesicles that
go to various cell destinations.
● Secretory vesicles carry products to the
membrane where exocytosis produces
secretions.
● Lysosomes fuse with incoming vesicles and
digest macromolecules.
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Endomembrane System: A Visual Summary
Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display.
secretio
n
plasma
membrane
enzyme
Golgi apparatus
modifies lipids and
lysosome proteins
contains digestive from the ER; sorts them
enzymes and packages them in
that break down worn-out vesicles
cell parts or substances
entering the cell at the protei
plasma membrane n
transport vesicle
transport vesicle shuttles proteins to
shuttles lipids to various locations such
various as
locations such as the the Golgi apparatus
Golgi apparatus
lipi
d
rough endoplasmic
smooth endoplasmic reticulum
reticulum synthesizes proteins and
synthesizes lipids packages them in
and vesicles;
also performs various vesicles commonly go to
other functions the Golgi apparatus
ribosom Nucleu
e s
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Vacuoles
● Membranous sacs that are larger than vesicles
● Store materials that occur in excess
● Others very specialized (contractile vacuole)
● Plants cells typically have a central vacuole
● Up to 90% volume of some cells
● Functions in:
● Storage of water, nutrients, pigments, and waste products
● Development of turgor pressure
● Some functions performed by lysosomes in other eukaryotes
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Vacuoles
Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display.
100
nm
© Newcomb/Wergin/Biological Photo Service
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Energy-Related Organelles: Chloroplasts
● Membranous organelles (a type of plastid) that serve as the site of
photosynthesis
● Captures light energy to drive cellular machinery
● Photosynthesis
● Synthesizes carbohydrates from CO2 & H2O
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Energy-Related Organelles: Chloroplasts
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Chloroplast Structure
Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display.
a 500
. nm
outer thylakoid
membran gran space strom thylakoid
double e a a membrane
inner
membran membran
e e
b
.
a: Courtesy Herbert W. Israel, Cornell University
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Energy-Related Organelles: Mitochondria
● Smaller than chloroplast
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Mitochondrial Structure
Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display.
a. 200 nm
outer
membran cristae matrix
double e
inner
membran
membran
e
e
b
.
a: Courtesy Dr. Keith Porter
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The Cytoskeleton
● Maintains cell shape
● Intermediate Filaments
● Microtubules
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The Cytoskeleton: Actin Filaments
● Extremely thin filaments like twisted pearl
necklace
● Dense web just under plasma membrane
maintains cell shape
● Support for microvilli in intestinal cells
● Intracellular traffic control
● For moving stuff around within cell
● Cytoplasmic streaming
● Function in pseudopods of amoeboid cells
● Pinch mother cell in two after animal mitosis
● Important component in muscle contraction (other
is myosin)
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The Cytoskeleton: Actin Filament Operation
Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display.
actin filament
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The Cytoskeleton: Intermediate Filaments
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The Cytoskeleton: Microtubules
● Hollow cylinders made of two globular proteins
called α and β tubulin
● Spontaneous pairing of α and β tubulin molecules
form structures called dimers
● Dimers then arrange themselves into tubular
spirals of 13 dimers around
● Assembly:
● Under control of Microtubule Organizing Center
(MTOC)
● Most important MTOC is centrosome
● Interacts with proteins kinesin and dynein to
cause movement of organelles
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The Cytoskeleton
Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display.
actin
subunit
Chara
a. Actin filaments
fibrous
subunits
peacock
b. Intermediate filaments
tubulin
dimer
chameleon
c. Microtubules
a(Actin): © M. Schliwa/Visuals Unlimited; b, c(Intermediate, Microtubules): © K.G. Murti/Visuals Unlimited; a(Chara): The McGraw-Hill Companies, Inc./photo by
Dennis Strete and Darrell Vodopich; b(Peacock): © Vol. 86/Corbis; c(Chameleon): © Photodisc/Vol. 6/Getty Images
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Microtubular Arrays: Cilia and Flagella
● Hair-like projections from cell surface that aid in
cell movement
● Very different from prokaryotic flagella
● Outer covering of plasma membrane
● Inside this is a cylinder of 18 microtubules arranged in
9 pairs
● In center are two single microtubules
● This 9 + 2 pattern used by all cilia & flagella
● In eukaryotes, cilia are much shorter than flagella
● Cilia move in coordinated waves like oars
● Flagella move like a propeller or cork screw
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Structure of a Flagellum
Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display.
outer
Flagellu microtubul
m e
doublet
radial
spoke
central
shaft The shaft of the microtubule
flagellum has a s
ring
of nine microtubule dynein
doublets anchored side arm
to a central pair of
microtubules.
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Flagellum cross
nm
section
Basal body
ATP
In the presence of
ATP, the dynein side
arms reach out to
their neighbors,
The basal body of a flagellum and bending occurs.
100 nm has
Basal body cross section a ring of nine microtubule triplets
with no central microtubules.
(Flagellum, Basal body): © William L. Dentler/Biological Photo Service
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Comparison of Prokaryotic and
Eukaryotic Cells
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ACTIVITY #1
https://forms.gle/9mryx9QwsR2UALjq6
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Review
● Cellular Level of Organization
● Cell theory
● Cell size
● Prokaryotic Cells
● Eukaryotic Cells
● Organelles
● Nucleus and Ribosome
● Endomembrane System
● Other Vesicles and Vacuoles
● Energy related organelles
● Cytoskeleton
● Cilia, and Flagella
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ASSIGNMENT
● Procedure: In your short bond paper
answer the test below.
https://studylib.net/doc/14980902/quiz-on-c
ell-virus
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Cell Structure and Function
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