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Micropropagation: " The Art and Science of Multiplying Plants in Vitro."

Micropropagation is the process of multiplying plants in vitro or in controlled laboratory conditions. It allows for rapid multiplication of plant clones from a single plant through tissue culture techniques. Key advantages include producing many genetically identical plants quickly, in controlled conditions year-round, and with the potential for disease-free plants. Specialized equipment and technical expertise is required compared to conventional propagation methods. Micropropagation has commercial applications for rapidly increasing stocks of new varieties, eliminating diseases, and cloning plants that are difficult to propagate through conventional methods.

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49 views

Micropropagation: " The Art and Science of Multiplying Plants in Vitro."

Micropropagation is the process of multiplying plants in vitro or in controlled laboratory conditions. It allows for rapid multiplication of plant clones from a single plant through tissue culture techniques. Key advantages include producing many genetically identical plants quickly, in controlled conditions year-round, and with the potential for disease-free plants. Specialized equipment and technical expertise is required compared to conventional propagation methods. Micropropagation has commercial applications for rapidly increasing stocks of new varieties, eliminating diseases, and cloning plants that are difficult to propagate through conventional methods.

Uploaded by

hafsa
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd
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Micropropagation

“… the art and


science of
multiplying
plants in vitro.”
Conventional Propagation
• Cuttings
• Budding, grafting
• Layering
Conventional Propagation
Advantages

• Equipment costs minimal


• Little experience or technical expertise needed
• Inexpensive
• Specialized techniques for growth control (e.g.
grafting onto dwarfing rootstocks)
Clone
Genetically identical assemblage of
individuals propagated entirely by
vegetative means from a single plant.
Rapid clonal in vitro propagation of plants:
•from cells, tissues or organs
•cultured aseptically on defined media
•contained in culture vessels
•maintained under controlled conditions of light
and temperature
Toward Commercial Micropropagation 1950s
Morel & Martin 1952
Meristem-tip culture for disease elimination
Morel 1960 Disease
eradication
Wimber 1963 & in vitro
production of
orchids
Commercialization of Micropropagation 1970s &
1980s
Murashige 1974
Broad commercial application
Micropropagation
Advantages

• From one to many propagules rapidly


• Multiplication in controlled lab conditions
• Continuous propagation year round
• Potential for disease-free propagules
• Inexpensive per plant once established
• Precise crop production scheduling
• Reduce stock plant space
• Long-term germplasm storage
• Production of difficult-to-propagate species
Micropropagation
Disadvantages

• Specialized equipment/facilities required


• More technical expertise required
• Protocols not optimized for all species
• Plants produced may not fit industry standards
• Relatively expensive to set up?
Micropropagation
Applications

• Rapid increase of stock of new varieties


• Elimination of diseases
• Cloning of plant types not easily propagated by
conventional methods (few offshoots/ sprouts/
seeds; date palms, ferns, nandinas)
• Propagules have enhanced growth features
(multibranched character; Ficus, Syngonium)

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