PowerPoint® Lecture Slides

prepared by
Barbara Heard,
Atlantic Cape Community
College

CHAPTER 25
The Urinary
System:
Part A

© Annie Leibovitz/Contact Press Images © 2013 Pearson Education, Inc.

 Kidney Functions

• Regulating total water volume and total

solute concentration in water
• Regulating ECF ion concentrations
• Ensuring long-term acid-base balance
• Removal of metabolic wastes, toxins,
drugs

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 Kidney Functions

• Endocrine functions
– Renin - regulation of blood pressure
– Erythropoietin - regulation of RBC
production
• Activation of vitamin D
• Gluconeogenesis during prolonged fasting

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 Urinary System Organs

• Kidneys - major excretory organs

• Ureters - transport urine from kidneys to
urinary bladder
• Urinary bladder - temporary storage
reservoir for urine
• Urethra transports urine out of body

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 Figure 25.1 The urinary system.

Hepatic veins (cut)

Esophagus (cut)
Inferior vena cava

Renal artery
Adrenal gland
Renal hilum
Aorta Renal vein

Kidney
Iliac crest
Ureter

Rectum (cut)
Uterus (part of female
reproductive system)
Urinary
bladder

Urethra

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 Figure 25.2b Position of the kidneys against the posterior body wall.

12th rib

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 Internal Anatomy

• Renal cortex
– Granular-appearing superficial region
• Renal medulla
– Composed of cone-shaped medullary (renal)
pyramids
– Pyramids separated by renal columns
• Inward extensions of cortical tissue

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 Homeostatic Imbalance

• Pyelitis
– Infection of renal pelvis and calyces
• Pyelonephritis
– Infection/inflammation of entire kidney
• Normally - successfully treated with
antibiotics

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 Figure 25.2a Position of the kidneys against the posterior body wall.

Anterior
Inferior
vena cava
Aorta
Peritoneum Peritoneal cavity
(organs removed)

Supportive
Renal tissue layers
vein
• Renal fascia
anterior
Renal
artery posterior
• Perirenal
fat capsule
• Fibrous
Body of capsule
vertebra L2
Body wall
Posterior

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 Figure 25.3 Internal anatomy of the kidney.

Renal
hilum

Renal cortex

Renal medulla

Major calyx
Papilla of
pyramid
Renal pelvis
Minor calyx
Ureter

Renal pyramid in
renal medulla
Renal column

Fibrous capsule

Photograph of right kidney, frontal section Diagrammatic view

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 Blood and Nerve Supply

• Kidneys cleanse blood; adjust its

composition  rich blood supply
• Renal arteries deliver ~ ¼ (1200 ml) of
cardiac output to kidneys each minute
• Arterial flow into and venous flow out of
kidneys follow similar paths
• Nerve supply via sympathetic fibers from
renal plexus

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 Figure 25.4a Blood vessels of the kidney.
Cortical radiate
vein
Cortical radiate
artery
Arcuate vein
Arcuate artery
Interlobar vein
Interlobar artery
Segmental arteries
Renal vein

Renal artery
Renal pelvis

Ureter

Renal medulla

Renal cortex

Frontal section illustrating major blood vessels

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 Figure 25.4b Blood vessels of the kidney.
Aorta Inferior vena cava

Renal artery Renal vein

Segmental artery Interlobar vein

Interlobar artery Arcuate vein

Arcuate artery Cortical radiate vein

Cortical radiate artery Peritubular

capillaries
or vasa recta

Afferent arteriole Efferent arteriole

Glomerulus (capillaries)

Nephron-associated blood vessels

(see Figure 25.7)
© 2013 Pearson Education, Inc. (b) Path of blood flow through renal blood vessels
 Nephrons

• Structural and functional units that form

urine
• > 1 million per kidney
• Two main parts
– Renal corpuscle
– Renal tubule

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 Renal Corpuscle

• Two parts of renal corpuscle

– Glomerulus
• Tuft of capillaries; fenestrated endothelium 
highly porous  allows filtrate formation
– Glomerular capsule (Bowman's capsule)
• Cup-shaped, hollow structure surrounding
glomerulus

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 Figure 25.5 Location and structure of nephrons.
Renal cortex
Renal medulla
Renal pelvis Glomerular capsule: parietal layer
Basement
membrane
Ureter Podocyte
Kidney
Fenestrated endothelium
Renal corpuscle of the glomerulus
• Glomerular capsule Glomerular capsule: visceral layer
• Glomerulus Distal
convoluted Apical Mitochondria
tubule microvilli

Proximal Highly infolded basolateral

convoluted membrane
tubule
Proximal convoluted tubule cells
Cortex Apical side

Medulla
Thick Basolateral side
Thin segment segment Distal convoluted tubule cells
Nephron loop
• Descending limb
• Ascending limb Nephron loop (thin-segment) cells
Collecting
duct Principal Intercalated cell
cell

Collecting duct cells

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 Renal Corpuscle

• Glomerular capsule
– Parietal layer - simple squamous epithelium
– Visceral layer - branching epithelial
podocytes
• Extensions terminate in foot processes that cling
to basement membrane
• Filtration slits between foot processes allow
filtrate to pass into capsular space

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 Figure 25.5 Location and structure of nephrons. (3 of 7)

Basement
membrane

Podocyte

Fenestrated endothelium
of the glomerulus

Glomerular capsule: visceral layer

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 Renal Tubule

• Three parts
– Proximal convoluted tubule
• Proximal  closest to renal corpuscle
– Nephron loop
– Distal convoluted tubule
• Distal  farthest from renal corpuscle

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 Renal Tubule

• Proximal convoluted tubule (PCT)

– Cuboidal cells with dense microvilli (brush
border  surface area); large mitochondria
– Functions in reabsorption and secretion
– Confined to cortex

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 Figure 25.5 Location and structure of nephrons. (4 of 7)

Apical microvilli Mitochondria

Highly infolded
basolateral membrane
Proximal convoluted tubule cells

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 Renal Tubule

• Nephron loop
– Descending and ascending limbs
– Proximal descending limb continuous with
proximal tubule
– Distal descending limb = descending thin
limb; simple squamous epithelium
– Thick ascending limb
• Cuboidal to columnar cells; thin in some nephrons

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 Figure 25.5 Location and structure of nephrons. (6 of 7)

Nephron loop (thin-segment) cells

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 Renal Tubule

• Distal convoluted tubule (DCT)

– Cuboidal cells with very few microvilli
– Function more in secretion than reabsorption
– Confined to cortex

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 Figure 25.5 Location and structure of nephrons. (5 of 7)

Apical side

Basolateral side
Distal convoluted tubule cells

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 Collecting Ducts

• Two cell types

– Principal cells
• Sparse, short microvilli
• Maintain water and Na+ balance
– Intercalated cells
• Cuboidal cells; abundant microvilli; two types
– A and B; both help maintain acid-base balance of blood

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 Figure 25.5 Location and structure of nephrons. (7 of 7)

Principal cell Intercalated cell

Collecting duct cells

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 Collecting Ducts

• Receive filtrate from many nephrons

• Run through medullary pyramids 
striped appearance
• Fuse together to deliver urine through
papillae into minor calyces

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 Figure 25.5 Location and structure of nephrons.
Renal cortex
Renal medulla
Renal pelvis Glomerular capsule: parietal layer
Basement
membrane
Ureter Podocyte
Kidney
Fenestrated endothelium
Renal corpuscle of the glomerulus
• Glomerular capsule Glomerular capsule: visceral layer
• Glomerulus Distal
convoluted Apical Mitochondria
tubule microvilli

Proximal Highly infolded basolateral

convoluted membrane
tubule
Proximal convoluted tubule cells
Cortex Apical side

Medulla
Thick Basolateral side
Thin segment segment Distal convoluted tubule cells
Nephron loop
• Descending limb
• Ascending limb Nephron loop (thin-segment) cells
Collecting
duct Principal Intercalated cell
cell

Collecting duct cells

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 Classes of Nephrons

• Cortical nephrons—85% of nephrons;

almost entirely in cortex
• Juxtamedullary nephrons
– Long nephron loops deeply invade medulla
– Ascending limbs have thick and thin
segments
– Important in production of concentrated urine

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 Figure 25.7a Blood vessels of cortical and juxtamedullary nephrons.
Cortical nephron Juxtamedullary nephron
• Short nephron loop • Long nephron loop
• Glomerulus further from the cortex-medulla junction • Glomerulus closer to the cortex-medulla junction
• Efferent arteriole supplies peritubular capillaries • Efferent arteriole supplies vasa recta
Renal Glomerulus Efferent
corpuscle (capillaries) arteriole Cortical radiate vein
Glomerular Cortical radiate artery
capsule Afferent arteriole
Collecting duct
Proximal Distal convoluted tubule
convoluted Afferent
tubule arteriole Efferent
arteriole

Peritubular
capillaries
Ascending
limb of
nephron loop

Cortex-medulla
junction
Arcuate vein
Kidney Vasa recta
Arcuate artery
Nephron loop
Descending
limb of
nephron loop

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 Nephron Capillary Beds

• Renal tubules associated with two

capillary beds
– Glomerulus
– Peritubular capillaries
• Juxtamedullary nephrons also associated
with
– Vasa recta

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 Nephron Capillary Beds

• Glomerulus - specialized for filtration

• Different from other capillary beds – fed
and drained by arteriole
– Afferent arteriole  glomerulus  efferent
arteriole
• Blood pressure in glomerulus high
because
– Afferent arterioles larger in diameter than
efferent arterioles
– Arterioles are high-resistance vessels

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 Nephron Capillary Beds

• Peritubular capillaries
– Low-pressure, porous capillaries adapted for
absorption of water and solutes
– Arise from efferent arterioles
– Cling to adjacent renal tubules in cortex
– Empty into venules

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 Nephron Capillary Beds

• Vasa recta
– Long, thin-walled vessels parallel to long
nephron loops of juxtamedullary nephrons
– Arise from efferent arterioles serving
juxtamedullary nephrons
• Instead of peritubular capillaries
– Function in formation of concentrated urine

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 Figure 25.7a Blood vessels of cortical and juxtamedullary nephrons.
Cortical nephron Juxtamedullary nephron
• Short nephron loop • Long nephron loop
• Glomerulus further from the cortex-medulla junction • Glomerulus closer to the cortex-medulla junction
• Efferent arteriole supplies peritubular capillaries • Efferent arteriole supplies vasa recta
Renal Glomerulus Efferent
corpuscle (capillaries) arteriole Cortical radiate vein
Glomerular Cortical radiate artery
capsule Afferent arteriole
Collecting duct
Proximal Distal convoluted tubule
convoluted Afferent
tubule arteriole Efferent
arteriole

Peritubular
capillaries
Ascending
limb of
nephron loop

Cortex-medulla
junction
Arcuate vein
Kidney Vasa recta
Arcuate artery
Nephron loop
Descending
limb of
nephron loop

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 Juxtaglomerular Complex (JGC)

• One per nephron

• Involves modified portions of
– Distal portion of ascending limb of nephron
loop
– Afferent (sometimes efferent) arteriole
• Important in regulation of rate of filtrate
formation and blood pressure

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 Juxtaglomerular Complex (JGC)

• Three cell populations

– Macula densa, granular cells, extraglomerular
mesangial cells
• Macula densa
– Tall, closely packed cells of ascending limb
– Chemoreceptors; sense NaCl content of
filtrate

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 Juxtaglomerular Complex (JGC)

• Granular cells (juxtaglomerular, or JG

cells)
– Enlarged, smooth muscle cells of arteriole
– Secretory granules contain enzyme renin
– Mechanoreceptors; sense blood pressure in
afferent arteriole

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 Juxtaglomerular Complex (JGC)

• Extraglomerular mesangial cells

– Between arteriole and tubule cells
– Interconnected with gap junctions
– May pass signals between macula densa and
granular cells

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 Figure 25.8 Juxtaglomerular complex (JGC) of a nephron.

Glomerular
capsule Glomerulus Foot
Efferent processes
arteriole Parietal layer of podocytes
of glomerular
capsule Podocyte cell body
(visceral layer)
Capsular
Afferent space Red blood cell
arteriole Efferent Proximal
arteriole tubule cell

Juxtaglomerular
complex
• Macula densa
cells
of the ascending
limb of nephron loop
Lumens of
• Extraglomerular glomerular
mesangial cells capillaries
• Granular
cells Endothelial cell
Afferent of glomerular
arteriole capillary
Glomerular mesangial
cells

Juxtaglomerular complex Renal corpuscle

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 Kidney Physiology: Mechanisms of Urine
Formation
• 180 L fluid processed daily; only 1.5 L 
urine
• Three processes in urine formation and
adjustment of blood composition
– Glomerular filtration
– Tubular reabsorption
– Tubular secretion

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 Kidney Physiology: Mechanisms of Urine
Formation
• Glomerular filtration – produces cell- and
protein-free filtrate
• Tubular reabsorption
– Selectively returns 99% of substances from
filtrate to blood in renal tubules and collecting
ducts
• Tubular secretion
– Selectively moves substances from blood to
filtrate in renal tubules and collecting ducts

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 Kidney Physiology: Mechanisms of Urine
Formation
• Kidneys filter body's entire plasma volume
60 times each day; consume 20-25%
oxygen used by body at rest; produce
urine from filtrate
• Filtrate (produced by glomerular filtration)
– Blood plasma minus proteins
• Urine
– <1% of original filtrate
– Contains metabolic wastes and unneeded
substances

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 Figure 25.9 A schematic, uncoiled nephron showing the three major renal processes that adjust plasma composition.

Afferent
arteriole Glomerular
capillaries

Efferent arteriole
Cortical
radiate
artery
Glomerular capsule
1

Renal tubule and

collecting duct
containing filtrate

Peritubular
2 capillary

To cortical radiate vein

Three major
renal processes: Urine
1 Glomerular filtration
2 Tubular reabsorption
3 Tubular secretion
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 Glomerular Filtration

• Passive process
• No metabolic energy required
• Hydrostatic pressure forces fluids and
solutes through filtration membrane
• No reabsorption into capillaries of
glomerulus

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 The Filtration Membrane

• Porous membrane between blood and

interior of glomerular capsule
– Water, solutes smaller than plasma proteins
pass; normally no cells pass
• Three layers
– Fenestrated endothelium of glomerular
capillaries
– Basement membrane (fused basal laminae
of two other layers)
– Foot processes of podocytes with filtration
slits; slit diaphragms repel macromolecules

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 Figure 25.10a The filtration membrane.

Glomerular
Efferent capsular space
Cytoplasmic extensions
arteriole of podocytes

Filtration slits

Podocyte
Afferent cell body
arteriole
Proximal
convoluted
Glomerular tubule
capillary covered by Parietal layer Fenestrations
podocytes that form of glomerular (pores)
the visceral layer of capsule
glomerular capsule
Glomerular capillaries and the
visceral layer of the glomerular Glomerular
capsule capillary endothelium
(podocyte covering Foot
and basement processes
membrane removed) of podocyte

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 Figure 25.10b The filtration membrane.
Filtration slits

Podocyte
cell body

Foot
processes

Filtration slits between the podocyte foot processes

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 Figure 25.10c The filtration membrane.
Filtration membrane
• Capillary endothelium
Capillary
• Basement membrane
• Foot processes of podocyte
of glomerular capsule

Filtration
slit
Slit
Plasma diaphragm
Filtrate
in capsular
space

Foot
Fenestration processes
(pore) of podocyte
Three layers of the filtration membrane
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 The Filtration Membrane

• Macromolecules "stuck" in filtration membrane

engulfed by glomerular mesangial cells
• Allows molecules smaller than 3 nm to pass
– Water, glucose, amino acids, nitrogenous wastes
• Plasma proteins remain in blood  maintains
colloid osmotic pressure  prevents loss of all
water to capsular space
– Proteins in filtrate indicate membrane problem

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 Pressures That Affect Filtration

• Outward pressures promote filtrate

formation
– Hydrostatic pressure in glomerular
capillaries = Glomerular blood pressure
• Chief force pushing water, solutes out of blood
• Quite high – 55 mm Hg (most capillary beds ~ 26
mm Hg)
– Because efferent arteriole is high resistance vessel with
diameter smaller than afferent arteriole

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 Pressures That Affect Filtration

• Inward forces inhibiting filtrate formation

– Hydrostatic pressure in capsular space (HP cs)
• Pressure of filtrate in capsule – 15 mm Hg
– Colloid osmotic pressure in capillaries (OP gc)
• "Pull" of proteins in blood – 30 mm Hg
• Sum of forces  Net filtration pressure
(NFP)
– 55 mm Hg forcing out; 45 mm Hg opposing =
net outward force of 10 mm Hg

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 Net Filtration Pressure (NFP)

• Pressure responsible for filtrate formation

(10 mm Hg)
• Main controllable factor determining
glomerular filtration rate (GFR)

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 Figure 25.11 Forces determining net filtration pressure (NFP).
Glomerular
capsule
Efferent
arteriole

HPgc = 55 mm Hg

OPgc = 30 mm Hg

Afferent HPcs = 15 mm Hg
arteriole

NFP = Net filtration pressure

= outward pressures – inward pressures
= (HPgc) – (HPcs + OPgc)
= (55) – (15 + 30)
= 10 mm Hg
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 Glomerular Filtration Rate (GFR)

• Volume of filtrate formed per minute by

both kidneys (normal = 120–125 ml/min)
• GFR directly proportional to
– NFP – primary pressure is hydrostatic
pressure in glomerulus
– Total surface area available for filtration –
glomerular mesangial cells control by
contracting
– Filtration membrane permeability – much
more permeable than other capillaries

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 Regulation of Glomerular Filtration

• Constant GFR allows kidneys to make

filtrate and maintain extracellular
homeostasis
– Goal of intrinsic controls - maintain GFR in
kidney
• GFR affects systemic blood pressure
  GFR  urine output   blood pressure,
and vice versa
– Goal of extrinsic controls - maintain systemic
blood pressure

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 Regulation of Glomerular Filtration

• Intrinsic controls (renal autoregulation)

– Act locally within kidney to maintain GFR
• Extrinsic controls
– Nervous and endocrine mechanisms that
maintain blood pressure; can negatively affect
kidney function
– Take precedence over intrinsic controls if
systemic BP < 80 or > 180 mm Hg

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 Regulation of Glomerular Filtration

• Controlled via glomerular hydrostatic

pressure
– If rises  NFP rises  GFR rises
– If falls only 18% GFR = 0

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 Intrinsic Controls

• Maintains nearly constant GFR when MAP

in range of 80–180 mm Hg
– Autoregulation ceases if out of that range
• Two types of renal autoregulation
– Myogenic mechanism
– Tubuloglomerular feedback mechanism

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 Intrinsic Controls: Myogenic Mechanism

• Smooth muscle contracts when stretched

  BP  muscle stretch  constriction of
afferent arterioles  restricts blood flow
into glomerulus
– Protects glomeruli from damaging high BP
  BP  dilation of afferent arterioles
• Both help maintain normal GFR despite
normal fluctuations in blood pressure

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 Intrinsic Controls: Tubuloglomerular
Feedback Mechanism
• Flow-dependent mechanism directed by
macula densa cells; respond to filtrate
NaCl concentration
• If GFR  filtrate flow rate  
reabsorption time  high filtrate NaCl
levels  constriction of afferent arteriole
  NFP & GFR  more time for NaCl
reabsorption
• Opposite for  GFR

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 Extrinsic Controls: Sympathetic Nervous
System
• Under normal conditions at rest
– Renal blood vessels dilated
– Renal autoregulation mechanisms prevail

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 Extrinsic Controls: Sympathetic Nervous
System
• If extracellular fluid volume extremely low
(blood pressure low)
– Norepinephrine released by sympathetic
nervous system; epinephrine released by
adrenal medulla 
• Systemic vasoconstriction  increased blood
pressure
• Constriction of afferent arterioles   GFR 
increased blood volume and pressure

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 Extrinsic Controls: Renin-Angiotensin-
Aldosterone Mechanism
• Main mechanism for increasing blood
pressure – see Chapters 16 and 19
• Three pathways to renin release by
granular cells
– Direct stimulation of granular cells by
sympathetic nervous system
– Stimulation by activated macula densa cells
when filtrate NaCl concentration low
– Reduced stretch of granular cells

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 Extrinsic Controls: Other Factors Affecting
GFR
• Kidneys release chemicals; some act as
paracrines that affect renal arterioles
– Adenosine
– Prostaglandin E2
– Intrinsic angiotensin II – reinforces effects of
hormonal angiotensin II

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 Tubular Reabsorption

• Most of tubular contents reabsorbed to

blood
• Selective transepithelial process
– ~ All organic nutrients reabsorbed
– Water and ion reabsorption hormonally
regulated and adjusted
• Includes active and passive tubular
reabsorption
• Two routes
– Transcellular or paracellular

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 Tubular Reabsorption

• Paracellular route
– Between tubule cells
• Limited by tight junctions, but leaky in proximal
nephron
– Water, Ca2+, Mg2+, K+, and some Na+ in the PCT

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 Figure 25.13 Transcellular and paracellular routes of tubular reabsorption. Slide 1
The transcellular route 3 Transport across the The paracellular route
involves: basolateral membrane. (Often involves:
involves the lateral intercellular • Movement through leaky
1 Transport across the spaces because membrane tight junctions, particularly in
apical membrane. transporters transport ions into the PCT.
2 Diffusion through the these spaces.) • Movement through the inter-
cytosol. 4 Movement through the inter- stitial fluid and into the
stitial fluid and into the capillary. capillary.
Filtrate Tubule cell Interstitial fluid
in tubule Peri-
lumen Tight junction Lateral tubular
intercellular capillary
space

3 4

H2O and 1 2 3 4
solutes
Transcellular Capillary
route endothelial
cell
Apical
membrane

Paracellular route
H2O and
solutes
Basolateral
membranes
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 Tubular Reabsorption of Sodium

• Na+ - most abundant cation in filtrate

– Transport across basolateral membrane
• Primary active transport out of tubule cell by
Na+-K+ ATPase pump  peritubular capillaries
– Transport across apical membrane
• Na+ passes through apical membrane by
secondary active transport or facilitated diffusion
mechanisms

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 Reabsorption of Nutrients, Water, and Ions

• Na+ reabsorption by primary active

transport provides energy and means for
reabsorbing most other substances
• Creates electrical gradient  passive
reabsorption of anions
• Organic nutrients reabsorbed by
secondary active transport; cotransported
with Na+
– Glucose, amino acids, some ions, vitamins

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 Passive Tubular Reabsorption of Water

• Movement of Na+ and other solutes

creates osmotic gradient for water
• Water reabsorbed by osmosis, aided by
water-filled pores called aquaporins
– Aquaporins always present in PCT 
obligatory water reabsorption
– Aquaporins inserted in collecting ducts only if
ADH present  facultative water
reabsorption

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 Passive Tubular Reabsorption of Solutes

• Solute concentration in filtrate increases

as water reabsorbed  concentration
gradients for solutes 
• Fat-soluble substances, some ions and
urea, follow water into peritubular
capillaries down concentration gradients
  Lipid-soluble drugs, environmental
pollutants difficult to excrete

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 Figure 25.14 Reabsorption by PCT cells. Slide 1

1 At the basolateral membrane, Na+ is 2 “Downhill” Na+ entry at the

pumped into the interstitial space by the apical membrane.
Na+-K+ ATPase. Active Na+ transport
3 Reabsorption of organic
creates concentration gradients that drive:
nutrients and certain ions by
Filtrate Nucleus Interstitial cotransport at the apical
Peri- membrane.
in tubule Tubule cell fluid tubular
lumen capillary 4 Reabsorption of water by
osmosis through
2 aquaporins. Water
Glucose reabsorption increases the
Amino 1 concentration of the
acids
Some 3 solutes that are left behind.
ions These solutes can then be
Vitamins reabsorbed as they move
4 down their gradients:
5 Lipid-soluble substances
Lipid-
soluble 5 diffuse by the transcellular
substances route.
Various 6
Ions 6 Various ions (e.g., Cl−,
Tight junction Paracellular Ca2+, K+) and urea diffuse
and urea route
by the paracellular route.
Primary active transport Transport protein
Secondary active transport Ion channel
Passive transport (diffusion) Aquaporin

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 Transport Maximum

• Transcellular transport systems specific

and limited
– Transport maximum (Tm) for ~ every
reabsorbed substance; reflects number of
carriers in renal tubules available
– When carriers saturated, excess excreted in
urine
• E.g., hyperglycemia  high blood glucose levels
exceed Tm  glucose in urine

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 Reabsorptive Capabilities of Renal Tubules
and Collecting Ducts
• PCT
– Site of most reabsorption
• All nutrients, e.g., glucose and amino acids
• 65% of Na+ and water
• Many ions
• ~ All uric acid; ½ urea (later secreted back into
filtrate)

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 Reabsorptive Capabilities of Renal Tubules
and Collecting Ducts
• Nephron loop
– Descending limb - H2O can leave; solutes
cannot
– Ascending limb – H2O cannot leave; solutes
can
• Thin segment – passive Na+ movement
• Thick segment – Na+-K+-2Cl- symporter and Na+-H+
antiporter; some passes by paracellular route

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 Reabsorptive Capabilities of Renal Tubules
and Collecting Ducts
• DCT and collecting duct
– Reabsorption hormonally regulated
• Antidiuretic hormone (ADH) – Water
• Aldosterone – Na+ (therefore water)
• Atrial natriuretic peptide (ANP) – Na+
• PTH – Ca2+

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 Reabsorptive Capabilities of Renal Tubules
and Collecting Ducts
• Antidiuretic hormone (ADH)
– Released by posterior pituitary gland
– Causes principal cells of collecting ducts to
insert aquaporins in apical membranes 
water reabsorption
• As ADH levels increase  increased water
reabsorption

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 Reabsorptive Capabilities of Renal Tubules
and Collecting Ducts
• Aldosterone
– Targets collecting ducts (principal cells) and
distal DCT
– Promotes synthesis of apical Na+ and K+
channels, and basolateral Na+-K+ ATPases for
Na+ reabsorption; water follows
  little Na+ leaves body; aldosterone absence
 loss of 2% filtered Na+ daily - incompatible
with life
– Functions – increase blood pressure;
decrease K+ levels

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 Reabsorptive Capabilities of Renal Tubules
and Collecting Ducts
• Atrial natriuretic peptide
– Reduces blood Na+  decreased blood
volume and blood pressure
– Released by cardiac atrial cells if blood
volume or pressure elevated
• Parathyroid hormone acts on DCT to
increase Ca2+ reabsorption

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 Tubular Secretion

• Reabsorption in reverse; almost all in PCT

– Selected substances
– K+, H+, NH4+, creatinine, organic acids and
bases move from peritubular capillaries
through tubule cells into filtrate
– Substances synthesized in tubule cells also
secreted – e.g., HCO3-

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 Tubular Secretion

• Disposes of substances (e.g., drugs)

bound to plasma proteins
• Eliminates undesirable substances
passively reabsorbed (e.g., urea and uric
acid)
• Rids body of excess K+ (aldosterone
effect)
• Controls blood pH by altering amounts of
H+ or HCO3– in urine

© 2013 Pearson Education, Inc.

 Figure 25.15 Summary of tubular reabsorption and secretion.

Cortex

65% of filtrate volume Regulated reabsorption

reabsorbed • Na+ (by aldosterone;
• H2O Cl− follows)
• Na+, HCO3−, and • Ca2+ (by parathyroid
many other ions hormone)
• Glucose, amino acids,
and other nutrients

• H+ and NH4+ Regulated

• Some drugs secretion
• K+ (by
aldosterone)

Outer Regulated
medulla reabsorption
• H2O (by ADH)
• Na+ (by
aldosterone; Cl−
follows)
• Urea (increased
by ADH)

• Urea

Regulated
secretion
Inner • K+ (by
medulla aldosterone)

• Reabsorption or secretion
to maintain blood pH
described in Chapter 26;
involves H+, HCO3−,
and NH4+

Reabsorption
Secretion
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 Regulation of Urine Concentration and
Volume
• Osmolality of body fluids
– Expressed in milliosmols (mOsm)
– Kidneys maintain osmolality of plasma at
~300 mOsm by regulating urine concentration
and volume
– Kidneys regulate with countercurrent
mechanism

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 Countercurrent Mechanism

• Occurs when fluid flows in opposite

directions in two adjacent segments of
same tube with hair pin turn
– Countercurrent multiplier – interaction of
filtrate flow in ascending/descending limbs of
nephron loops of juxtamedullary nephrons
– Countercurrent exchanger - Blood flow in
ascending/descending limbs of vasa recta

© 2013 Pearson Education, Inc.

 Figure 25.16a Juxtamedullary nephrons create an osmotic gradient within the renal medulla that allows the kidney to
produce urine of varying concentration. (1 of 4)

The three key players and their

orientation in the osmotic gradient:

(c) The collecting ducts of

all nephrons use the gradient
to adjust urine osmolality.

300

300

400
(a) The long nephron loops of
juxtamedullary nephrons create
the gradient. They act as 600
countercurrent multipliers.
The osmolality of the medullary
900 interstitial fluid progressively
increases from the 300 mOsm of
(b) The vasa recta preserve the normal body fluid to 1200 mOsm
gradient. They act as at the deepest part of the medulla.
countercurrent exchangers. 1200

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 The Countercurrent Multiplier

• Constant 200 mOsm difference between

two limbs of nephron loop and between
ascending limb and interstitial fluid
• Difference "multiplied" along length of loop
to ~ 900 mOsm

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 The Countercurrent Exchanger

• Vasa recta
• Preserve medullary gradient
– Prevent rapid removal of salt from interstitial
space
– Remove reabsorbed water
• Water entering ascending vasa recta
either from descending vasa recta or
reabsorbed from nephron loop and
collecting duct 
– Volume of blood at end of vasa recta greater
than at beginning
© 2013 Pearson Education, Inc.
 Figure 25.16a Juxtamedullary nephrons create an osmotic gradient within the renal medulla that allows the kidney to
produce urine of varying concentration. (4 of 4)

(continued) As water and solutes are reabsorbed, the loop first concentrates the filtrate, then dilutes it.

Active transport
Passive transport
Water impermeable 300
300 100

Cortex 100
300 300
1 Filtrate entering the 5 Filtrate is at its most dilute as it
nephron loop is isosmotic to leaves the nephron loop. At
both blood plasma and 100 mOsm, it is hypo-osmotic
Osmolality of interstitial fluid (mOsm)

cortical interstitial fluid. to the interstitial fluid.

400 400 200

4 Na+ and Cl- are pumped out

Outer of the filtrate. This increases the
medulla interstitial fluid osmolality.
600 600 400
2 Water moves out of the
filtrate in the descending limb
down its osmotic gradient.
This concentrates the filtrate.
900 900 700

3 Filtrate reaches its highest

concentration at the bend of the
Inner loop.
medulla Nephron loop
1200 1200

© 2013 Pearson Education, Inc.

 Figure 25.16b Juxtamedullary nephrons create an osmotic gradient within the renal medulla that allows the kidney to
produce urine of varying concentration.

Vasa recta preserve the gradient.

The entire length of the vasa recta is highly permeable to water
and solutes. Due to countercurrent exchanges between each
section of the vasa recta and its surrounding interstitial fluid, the
blood within the vasa recta remains nearly isosmotic to the
surrounding fluid. As a result, the vasa recta do not undo the
osmotic gradient as they remove reabsorbed water and solutes.

Blood from
efferent To vein
arteriole

300 325

300
400
The countercurrent
flow of fluid moves
400 through two adjacent
parallel sections of
the vasa recta.

600

600

900

900

1200
Vasa recta
© 2013 Pearson Education, Inc.
 Figure 25.16c Juxtamedullary nephrons create an osmotic gradient within the renal medulla that allows the kidney to
produce urine of varying concentration.

Collecting ducts use the gradient.

Under the control of antidiuretic hormone, the collecting
ducts determine the final concentration and volume of
urine. This process is fully described in Figure 25.17.

Collecting duct

300

Osmolality of interstitial fluid (mOsm)

400

600

900

1200
© 2013 Pearson Education, Inc. Urine
 Formation of Dilute or Concentrated Urine

• Osmotic gradient used to raise urine

concentration > 300 mOsm to conserve
water
– Overhydration  large volume dilute urine
• ADH production ; urine ~ 100 mOsm
• If aldosterone present, additional ions removed 
~ 50 mOsm
– Dehydration  small volume concentrated
urine
• Maximal ADH released; urine ~ 1200 mOsm
• Severe dehydration – 99% water reabsorbed

© 2013 Pearson Education, Inc.

 Figure 25.17 Mechanism for forming dilute or concentrated urine.
If we were so overhydrated we had no ADH... If we were so dehydrated we had maximal ADH...

Osmolality of extracellular fluids Osmolality of extracellular fluids

ADH release from posterior pituitary ADH release from posterior pituitary
Number of aquaporins (H2O channels) in collecting duct Number of aquaporins (H2O channels) in collecting duct

H2O reabsorption from collecting duct H2O reabsorption from collecting duct

Large volume of dilute urine Small volume of concentrated urine

Collecting Collecting duct

duct
Descending limb 300 Descending limb 300
of nephron loop of nephron loop
100 100 150

DCT 100 DCT 300

Osmolality of interstitial fluid (mOsm)

Osmolality of interstitial fluid (mOsm)

Cortex Cortex
300 100 300 300 100 300 300

400

600 400 600 600 400 600 600

100
Outer Outer
medulla medulla

Urea
900 700 900 900 700 900 900

Urea Urea
Inner Inner
medulla medulla
1200 100 1200 1200
1200 1200

Large volume Small volume of

of dilute urine Urea contributes to concentrated urine
Active transport the osmotic gradient.
Passive transport ADH increases its
recycling.

© 2013 Pearson Education, Inc.

 Urea Recycling and the Medullary Osmotic
Gradient
• Urea helps form medullary gradient
– Enters filtrate in ascending thin limb of
nephron loop by facilitated diffusion
– Cortical collecting duct reabsorbs water;
leaves urea
– In deep medullary region now highly
concentrated urea  interstitial fluid of
medulla  back to ascending thin limb 
high osmolality in medulla

© 2013 Pearson Education, Inc.

 Diuretics

• Chemicals that enhance urinary output

– ADH inhibitors, e.g., alcohol
– Na+ reabsorption inhibitors (and resultant H 2O
reabsorption), e.g., caffeine, drugs for
hypertension or edema
– Loop diuretics inhibit medullary gradient
formation
– Osmotic diuretics - substance not reabsorbed
so water remains in urine, e.g., high glucose
of diabetic patient

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 Renal Clearance

• Volume of plasma kidneys clear of

particular substance in given time
• Renal clearance tests used to determine
GFR
– To detect glomerular damage
– To follow progress of renal disease

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 Renal Clearance

• C = UV/P
– C = renal clearance rate (ml/min)
– U = concentration (mg/ml) of substance in
urine
– V = flow rate of urine formation (ml/min)
– P = concentration of same substance in
plasma

© 2013 Pearson Education, Inc.

 Renal Clearance

• Inulin (plant polysaccharide) is standard used

– Freely filtered; neither reabsorbed nor secreted by
kidneys; its renal clearance = GFR = 125 ml/min
• If C < 125 ml/min, substance reabsorbed
• If C = 0, substance completely reabsorbed, or
not filtered
• If C = 125 ml/min, no net reabsorption or
secretion
• If C > 125 ml/min, substance secreted (most
drug metabolites)

© 2013 Pearson Education, Inc.

 Homeostatic Imbalance

• Chronic renal disease - GFR < 60 ml/min

for 3 months
– E.g., in diabetes mellitus; hypertension
• Renal failure – GFR < 15 ml/min
– Causes uremia – ionic and hormonal
imbalances; metabolic abnormalities; toxic
molecule accumulation
– Treated with hemodialysis or transplant

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 Physical Characteristics of Urine

• Color and transparency

– Clear
• Cloudy may indicate urinary tract infection
– Pale to deep yellow from urochrome
• Pigment from hemoglobin breakdown; more
concentrated urine  deeper color
– Abnormal color (pink, brown, smoky)
• Food ingestion, bile pigments, blood, drugs

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 Physical Characteristics of Urine

• Odor
– Slightly aromatic when fresh
– Develops ammonia odor upon standing
• As bacteria metabolize solutes
– May be altered by some drugs and
vegetables

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 Physical Characteristics of Urine

• pH
– Slightly acidic (~pH 6, with range of 4.5 to 8.0)
• Acidic diet (protein, whole wheat)   pH
• Alkaline diet (vegetarian), prolonged vomiting, or
urinary tract infections  pH
• Specific gravity
– 1.001 to 1.035; dependent on solute
concentration

© 2013 Pearson Education, Inc.

 Chemical Composition of Urine

• 95% water and 5% solutes

• Nitrogenous wastes
– Urea (from amino acid breakdown) – largest
solute component
– Uric acid (from nucleic acid metabolism)
– Creatinine (metabolite of creatine phosphate)

© 2013 Pearson Education, Inc.

 Chemical Composition of Urine

• Other normal solutes

– Na+, K+, PO43–, and SO42–, Ca2+, Mg2+ and
HCO3–
• Abnormally high concentrations of any
constituent, or abnormal components, e.g.,
blood proteins, WBCs, bile pigments, may
indicate pathology

© 2013 Pearson Education, Inc.

 Urine transport, Storage, and Elimination:
Ureters
• Convey urine from kidneys to bladder
– Begin at L2 as continuation of renal pelvis
• Retroperitoneal
• Enter base of bladder through posterior
wall
– As bladder pressure increases, distal ends of
ureters close, preventing backflow of urine

© 2013 Pearson Education, Inc.

 Homeostatic Imbalance

• Renal calculi - kidney stones in renal

pelvis
– Crystallized calcium, magnesium, or uric acid
salts
• Large stones block ureter  pressure &
pain
• May be due to chronic bacterial infection,
urine retention, Ca2+ in blood, pH of
urine
• Treatment - shock wave lithotripsy –
noninvasive; shock waves shatter calculi
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 Urinary Bladder

• Muscular sac for temporary storage of

urine
• Retroperitoneal, on pelvic floor posterior to
pubic symphysis
– Males—prostate inferior to bladder neck
– Females—anterior to vagina and uterus

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 Urinary Bladder

• Openings for ureters and urethra

• Trigone
– Smooth triangular area outlined by openings
for ureters and urethra
– Infections tend to persist in this region

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 Urinary Bladder

• Layers of bladder wall

– Mucosa - transitional epithelial mucosa
– Thick detrusor - three layers of smooth
muscle
– Fibrous adventitia (peritoneum on superior
surface only)

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 Urinary Bladder

• Collapses when empty; rugae appear

• Expands and rises superiorly during filling
without significant rise in internal pressure
• ~ Full bladder 12 cm long; holds ~ 500 ml
– Can hold ~ twice that if necessary
– Can burst if overdistended

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 Figure 25.18 Pyelogram.

Kidney

Renal
pelvis

Ureter

Urinary
bladder

© 2013 Pearson Education, Inc.

 Figure 25.20a Structure of the urinary bladder and urethra.
Peritoneum
Ureter
Rugae
Detrusor
Adventitia
Ureteric orifices
Trigone of bladder
Bladder neck
Internal urethral sphincter
Prostate
Prostatic urethra
Intermediate part of the urethra
External urethral sphincter
Urogenital diaphragm

Spongy urethra
Erectile tissue of penis

External urethral orifice

Male. The long male urethra has three regions:
prostatic, intermediate, and spongy.
© 2013 Pearson Education, Inc.
 Figure 25.20b Structure of the urinary bladder and urethra.
Peritoneum

Ureter

Rugae
Detrusor

Ureteric orifices

Bladder neck

Internal urethral
sphincter

Trigone

External urethral
sphincter
Urogenital diaphragm Urethra
External urethral
orifice
Female.
© 2013 Pearson Education, Inc.
 Urethra

• Sphincters
– Internal urethral sphincter
• Involuntary (smooth muscle) at bladder-urethra
junction
• Contracts to open
– External urethral sphincter
• Voluntary (skeletal) muscle surrounding urethra as
it passes through pelvic floor

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 Urethra

• Female urethra (3–4 cm)

– Tightly bound to anterior vaginal wall
– External urethral orifice
• Anterior to vaginal opening; posterior to clitoris

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 Urethra

• Male urethra carries semen and urine

– Three named regions
• Prostatic urethra (2.5 cm)—within prostate
• Intermediate part of the urethra (membranous
urethra) (2 cm)—passes through urogenital
diaphragm from prostate to beginning of penis
• Spongy urethra (15 cm)—passes through penis;
opens via external urethral orifice

© 2013 Pearson Education, Inc.

 Figure 25.20a Structure of the urinary bladder and urethra.
Peritoneum
Ureter
Rugae
Detrusor
Adventitia
Ureteric orifices
Trigone of bladder
Bladder neck
Internal urethral sphincter
Prostate
Prostatic urethra
Intermediate part of the urethra
External urethral sphincter
Urogenital diaphragm

Spongy urethra
Erectile tissue of penis

External urethral orifice

Male. The long male urethra has three regions:
prostatic, intermediate, and spongy.
© 2013 Pearson Education, Inc.
 Micturition

• Urination or voiding
• Three simultaneous events must occur
– Contraction of detrusor by ANS
– Opening of internal urethral sphincter by ANS
– Opening of external urethral sphincter by
somatic nervous system

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 Micturition

• Reflexive urination (urination in infants)

– Distension of bladder activates stretch
receptors
– Excitation of parasympathetic neurons in
reflex center in sacral region of spinal cord
– Contraction of detrusor
– Contraction (opening) of internal sphincter
– Inhibition of somatic pathways to external
sphincter, allowing its relaxation (opening)

© 2013 Pearson Education, Inc.

 Figure 25.21 Control of micturition.

Brain
Higher brain
Urinary bladder centers
fills, stretching
bladder wall
Allow or inhibit micturition
as appropriate

Afferent impulses Pontine micturition Pontine storage

from stretch center center
receptors

Simple Promotes micturition Inhibits micturition by

spinal by acting on all three acting on all three
reflex spinal efferents Spinal efferents

Spinal Spinal
cord cord

Parasympathetic Sympathetic Somatic motor Parasympathetic activity

activity activity nerve activity Sympathetic activity
Somatic motor nerve activity

Detrusor contracts; External urethral

internal urethral sphincter opens
sphincter opens

Inhibits
Micturition

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 Homeostatic Imbalance

• Incontinence usually from weakened

pelvic muscles
– Stress incontinence
• Increased intra-abdominal pressure forces urine
through external sphincter
– Overflow incontinence
• Urine dribbles when bladder overfills

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 Homeostatic Imbalance

• Urinary retention
– Bladder unable to expel urine
– Common after general anesthesia
– Hypertrophy of prostate
– Treatment - catheterization

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 Developmental Aspects

• Three sets of embryonic kidneys form in

succession
– Pronephros degenerates but pronephric
duct persists
– Mesonephros claims this duct; becomes
mesonephric duct
– Metanephros develop by fifth week, develops
into adult kidneys and ascends

© 2013 Pearson Education, Inc.

 Figure 25.22a Development of the urinary system in the embryo.

Degenerating
pronephros Developing
digestive tract
Urogenital Duct to
ridge yolk sac
Mesonephros Allantois

Cloaca
Mesonephric duct Ureteric bud
(initially, pronephric duct)
Hindgut
Week 5

© 2013 Pearson Education, Inc.

 Figure 25.22b Development of the urinary system in the embryo.

Degenerating
pronephros

Duct to yolk sac

Allantois
Body stalk

Urogenital sinus
Rectum
Mesonephros Ureteric bud
Mesonephric
duct Metanephros
Week 6
© 2013 Pearson Education, Inc.
 Developmental Aspects

• Metanephros develops as ureteric buds

that induce mesoderm of urogenital ridge
to form nephrons
– Distal ends of ureteric buds form renal pelves,
calyces, and collecting ducts
– Proximal ends become ureters
• Kidneys excrete urine into amniotic fluid by
third month
• Cloaca subdivides into rectum, anal canal,
and urogenital sinus

© 2013 Pearson Education, Inc.

 Figure 25.22c Development of the urinary system in the embryo.

Urogenital
sinus
Gonad (developing
urinary
bladder)
Rectum
Metanephros
(kidney)

Week 7

© 2013 Pearson Education, Inc.

 Figure 25.22d Development of the urinary system in the embryo.

Urinary bladder
Gonad
Urethra
Kidney
Anus

Ureter Rectum

Week 8

© 2013 Pearson Education, Inc.

 Homeostatic Imbalance

• Three common congenital abnormalities

• Horseshoe kidney
– Two kidneys fuse across midline  single U-
shaped kidney; usually asymptomatic
• Hypospadias
– Urethral orifice on ventral surface of penis
– Corrected surgically at ~ 12 months

© 2013 Pearson Education, Inc.

 Homeostatic Imbalance

• Polycystic kidney disease

– Many fluid-filled cysts interfere with function
• Autosomal dominant form – less severe but more
common
• Autosomal recessive – more severe
– Cause unknown but involves defect in
signaling proteins

© 2013 Pearson Education, Inc.

 Developmental Aspects

• Frequent micturition in infants due to small

bladders and less-concentrated urine
• Incontinence normal in infants: control of
voluntary urethral sphincter develops with
nervous system
• E. coli bacteria account for 80% of all urinary
tract infections
• Untreated childhood streptococcal infections
may cause long-term renal damage
• Sexually transmitted diseases can also inflame
urinary tract

© 2013 Pearson Education, Inc.

 Developmental Aspects

• Most elderly people have abnormal

kidneys histologically
– Kidneys shrink; nephrons decrease in size
and number; tubule cells less efficient
– GFR ½ that of young adult by age 80
• Possibly from atherosclerosis of renal arteries
• Bladder shrinks; loss of bladder tone 
nocturia and incontinence

© 2013 Pearson Education, Inc.