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Use of Microscope New

The document discusses the use of microscopes, describing their history, basic terminology, how to operate one, and their applications. A microscope is an optical instrument that uses lenses to produce magnified images of small objects too small to see with the naked eye, and they are essential tools used across various fields like biology, medicine, and mineralogy to study cells, bacteria, diseases, and rock samples. Proper operation of a microscope involves adjusting settings like magnification, focus, light, and using lenses correctly to examine slides and samples.

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53 views

Use of Microscope New

The document discusses the use of microscopes, describing their history, basic terminology, how to operate one, and their applications. A microscope is an optical instrument that uses lenses to produce magnified images of small objects too small to see with the naked eye, and they are essential tools used across various fields like biology, medicine, and mineralogy to study cells, bacteria, diseases, and rock samples. Proper operation of a microscope involves adjusting settings like magnification, focus, light, and using lenses correctly to examine slides and samples.

Uploaded by

sa72562624742
Copyright
© © All Rights Reserved
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd
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USE OF MICROSCOPE

M icrobiology
BY: Hafiz Ahmad
Lubab ktk
Institute of Nursing
Sciences, KMU
Objectives
 Demonstrate and make use of operating a microscope.

 Follow the professional attributes and norms while using a


microscope.
What is a Microscope?

 MICROSCOPE (Greek: mikron = small and scopeos = to look): Is


an instrument for viewing objects that are too small to be seen by
the naked or unaided eye.
 Microscope is an optical instrument that uses a lens or combination
of lenses to produce magnified images of small objects, especially
objects too small to be seen by the unaided eye.

 MICROSCOPY: The science of investigating small objects


using such an instrument is called microscopy
History of Microscope
 Light microscope is one of the most basic and essential pieces of equipment
used in any laboratory.
 It is used for visualizing very small objects like cells, bacteria, parasite,
Ova/cyst etc.
 1590 - Hans Janssen and his son Zacharias Janssen, developed first
microscope.
 1667: Robert Hooke's famous "Micrographia" is published, which outlines
Hooke's various studies using the microscope
Basic Terminology
 MAGNIFICATION-Degree of enlargement:
No of times the length, breadth or diameter, of
an object is multiplied.

 TOTAL MAGNIFICATION: magnification of


the eyepiece x magnification of the objective.

 RESOLUTION: Ability to reveal closely


adjacent structural details as separate and
distinct.
Microscope
How to Operate
• 1.The microscope should be placed on a level bench, which should be
free of vibration.
• 2.The power socket to which the microscope is plugged should not be
loose or sparking.
• 3.The height of the microscope or chair should be adjusted in such a
way that the users eyes are directly on the eyepieces.
• 4.Place the specimen on the stage ,switch to the x10 objective and focus.
• 5.Turn on the power at the wall and the power switch on the microscope.
• 6.Ensure the light beam diaphragm is opened.
How to Operate
• 7.Start with the light setting at low to medium.
• 8.Depending on the sample being examined, the condenser may need to
be adjusted.
• 9.Secure the slide using the arms on the microscope stages.
• 10.When starting to examine a slide, select the low powered(X4) object
lens first.
• 11.Your eye level should be just above the eye pieces then look down the
eye pieces and gently slide them together until you see a single object.
• 12.Locate an area of the slide that includes part of the sample .
How to Operate
• 13.To bring the slide into focus rotate the coarse focus slowly until the
sample can be visualized clearly.
• 14.Once an area of interest has been identified, rotate through the objective
lenses to a higher power.
• 15.The oil immersion lens(and oil) is required to look at cell detail or
bacteria.
• 16.Rotate the objective lenses slightly leaving a space to add a drop of
immersion oil to the slide, directly over the spot of light.
How to Operate
• 17.Look down the eyepieces and use the fine focus to bring the image into
focus.
• 18.Once the examination is completed, remove the slide and clean the oil
from the immersion lens. Only use lens tissue on microscope objectives.
Application of Light Microscopy
 Light microscopes play a large part in today’s biology.
 Microscopes are used in biology to study diseases like cancer and AIDS to
help diagnose the disease in patients and to help find a cure for them.
 This device is used for observing microorganisms and their features. In this
field, microscopes are used to study bacteria, cells and many more. This
device helps biologists in their study of living organisms and their cell
structures.
 The main application of microscopes is scientific research.
Application of Light Microscopy
 Mineralogists also use light microscopy, typically with a special
preparation of a sample called thin section.
 As the name implies, thin section are very thin slices of a rock. The
sample needs to be thin enough for light to travel through from the light
source to the user’s eye.
 The thin section will allow the shape of different crystal grains to be seen.
Application of Light Microscopy

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