Parts and Functions of A Compound Microscope
Parts and Functions of A Compound Microscope
Compound Microscope
QUARTER 2 – MODULE 1
A Microscope is a tool that can help you see tiny
objects and single-celled organisms. It makes them
look bigger.
Its ability to make the specimen bigger is called
magnifying power or magnification. The
microscope also has the capacity to distinguish
small gaps between two separate points which
humans cannot distinguish. It is called its resolving
power or resolution.
The compound light microscope comes with
two sets of lenses which causes it to achieve a
higher level of magnification.
It uses diffused light from the sun or artificial
light to illuminate the object to be observed
through the glass lenses.
As light passes through the lenses, it is bent so the
specimen appears bigger when it is projected to the
eye. The form and structure of the specimen can
then be seen because some of their parts reflect
light. It is important to remember never to use
direct sunlight as a light source to view objects
since direct sunlight can permanently damage the
retina of the eye.
The parts of the compound microscope and its
corresponding functions are as follows;
1.Eyepiece or ocular- the lens at the top of the
microscope that you look through. On its rim, there are
certain markings such as 5x, 10x, 15x, which indicates the
magnification power.
2. Body tube- is a hollow tubular structure that connects
the eyepiece to the objective lenses. It can be shifted
down and up using the adjustment knobs.
3. Arm- the part that supports the body tube and
connects it to the base of the microscope.
4. Base- the bottom of the microscope used for
support. It also houses the illuminator or mirror.
5. Stage- the flat platform where slides are placed.
6. Stage clips- hold the slides in place.
7. Mirror- it is used to reflect light from an external light
source up to the diaphragm, object to be observed, and
lenses. Positioning the microscope towards diffused light
from the windows and ceiling one can see through the
eyepiece a bright circle of light called the field of view.
8.Revolving nosepiece- carries the objective lenses and
can be rotated to easily change power (magnification).
9. Fine adjustment knob- the small knob used for
focusing finer details of the specimen being
viewed.
10. Coarse adjustment knob- the large knob
located on the arm of the microscope used for
moving the body tube down and up for bringing the
object to be examined under exact focus.
11. Objective- a component that magnifies the images of
the specimen to form an enlarged image. There are three
(3) objective lenses, namely;
a.Oil immersion objective (OIO)-used to view bacteria,
very small protists, and fungi. It is marked 97x or 100x or
the word “oil”.
b.High power objective (HPO)- is marked 40x, 43x, or
60x.
c. Low power objective (LPO)- is marked 10x or 12x.
12. Inclination joint- a joint where the arm is
fastened to the base. It is used to tilt the microscope
for more comfortable viewing.
13. Diaphragm- is fastened below the stage. It
regulates the amount of light passing through the
specimen.
Proper care should be given to each part and the
microscope as a whole. Remember to always carry the
microscope with both hands, one is holding the arm and
the other is holding the base. Place it 17 centimeters away
from the edge of the table with the arm facing you. Always
hold it in an upright position. Before using the microscope,
wipe the metal parts with tissue paper or a piece of cloth
and wipe the lenses of the eyepiece and objectives with a
lens paper.
Label the parts of the microscope.
M
1
9
2
3 10
5M 4
6
7
8 11
12
13