(Lecture - 2) Cellular Organelles
(Lecture - 2) Cellular Organelles
CELLS
1
PROPERTIES OF EUKARYOTIC AND
PROKARYOTIC CELLS
2
REPRSENTATIVE
ANIMAL CELL
3
REPRESENTATIVE
PLANT CELL
4
TYPES OF
ORGANISMS
5
PLASMA
MEMBRANE
• Double layer of phospholipids and proteins
• Separates the interior of cell from outside environment
• Selectively permeable
• Helps in cell adhesion, ion conductivity, cell signalling
• serve as the attachment surface for extracellular structures
6
7
Phospholipid
s
• Polar
• Hydrophylic head
• Hydrophobic tail
8
MOVEMENT ACROSS THE PLASMA
MEMBRANE
• A few molecules move freely
• Water, Carbon dioxide, Ammonia, Oxygen
9
10
MEMBRANE
PROTEINS
1. Channels or transporters
• Move molecules in one direction
2. Receptors
• Recognize certain chemicals
11
MEMBRANE
PROTEINS
3. Glycoproteins
• Identify cell type
4. Enzymes
• Catalyze production of substances
12
MOLECULE MOVEMENT &
CELLS
• Passive Transport
• Active Transport
• Endocytosis
(phagocytosis & pinocytosis)
• Exocytosis
13
PASSIVE
TRANSPORT
• Involves movement along the concentration gradient
• No use of energy
14
Types of Passive
Transport
1. Diffusion
2. Osmosis
3. Facilitated diffusion
15
Diffusio
•nMolecules move to equalize concentration
16
Osmosi
s•
Special form of diffusion require the presence of a
semi-permeable membrane
17
Solution Differences &
Cells
• solvent + solute = solution
• Hypotonic
• Solutes in cell more than outside
• Outside solvent will flow into cell
• Isotonic
• Solutes equal inside & out of cell
• Hypertonic
• Solute conc. is greater outside cell
• Fluid will flow out of cell
18
19
Process of Facilitated
• Transport
Protein binds with molecule
• Shape of protein changes
• Molecule moves across membrane
20
Active
Transport
• Molecular movement against the concentration gradient(i.e
from lower concentration to higher concentration)
• Requires energy
• Example is sodium-potassium pump
21
Endocytosi
s • Movement of large material
• Particles
• Organisms
• Large molecules
• Movement is into cells
• Types of endocytosis
• bulk-phase (nonspecific)
• receptor-mediated (specific)
22
Forms of
Endocytosis
• Phagocytosis – cell eating
• Pinocytosis – cell drinking
23
Process of
Endocytosis
• Plasma membrane surrounds material
• Edges of membrane meet
• Membranes fuse to form vesicle
24
Exocytosi
s • Reverse of endocytosis
• Cell discharges material
25
Process of
Exocytosis
• Vesicle moves to cell surface
• Membrane of vesicle fuses
• Materials expelled
26
CYTOPLA
•SM
Occupies and fills the space inside a cell.
• Thick, gelatinous, semitransparent fluid present in both
eukaryotic and prokaryotic cells.
• It is enclosed in the plasma membrane.
• The cytoplasm has three major components - the
cytosol, organelles, and cytoplasmic inclusions.
27
Cytoso
l
• The Cytosol is the part of Cytoplasm that is not occupied by
any organelle.
• It is a gelatinous fluid, where other components of
the cytoplasm remain suspended.
• It mainly consists of cytoskeleton filaments, organic
molecules, salt, and water.
28
Structure of cytoskeleton
• The cytoskeleton is a network of fibers that forms the
"infrastructure" of cell
• The cytoskeleton is composed of at least three different types
of fibers : microtubules, microfilaments, and intermediate
filaments.
• These fibers are distinguished by their size with microtubules
being the thickest and microfilaments being the thinnest.
29
Functions of
cytoskeleton
• Helps the cell to maintain its shape and provides support to
the cell
• Helps in the formation of vacuole
• Keeps the cellular organelles in place
• Helps in formation of cellular appendages like cilia and
flagella
30
microtubules
•Microtubules – long hollow tubes
made of globular tubulin proteins
•Anchor organelles and act as tracks
for organelle movement
• Move chromosomes around during
cell
division
31
microfilaments
• Thinnest cytoskeletal elements
• Solid rod like structures
• Composed of the globular protein actin
• Enable cells to change shape and move
32
Intermediate filaments
• Fibrous proteins join to form a rope-like structure
• provide support for microfilaments and microtubules by
holding them in place.
33
NUCLE
US
•Genetic control center of a eukaryotic
cell.
•It is spherical, and the most prominent
part of the cell, making up 10% of the
cell’s volume.
•Unique structure and function that is
essential for the cell.
34
Functions of
•Nucleus
The “command center,” as it controls all functions of the cell.
• It is important in regulating the actions of the cells.
• Plays an important part in creating the cell’s proteins.
• It is involved in important processes dealing with DNA and other
genetic molecules.
35
Structure of
Nucleus
i. Nuclear envelope
ii. Nucleoplasm
iii. Chromatin
iv. Nucleolus
36
i. Nuclear envelope
(structure and function)
• Bilayer membrane
• Perforated
• Consists of ribosomes
• Regulates the movement of
material betwwen nucleoplasm
and cytoplasm
37
ii. Nucleoplasm
(structure and function)
• A jelly-like (made mostly of water) matrix within the nucleus
• All the other materials “float” inside
• Helps the nucleus keep its shape and serves as the median for
the transportation of important molecules within the nucleus
38
iii. Nucleolus
(structure and function)
• Largest structure present inside the boundaries of the nucleus
• More than one can be present in the cell’s nucleus
• Dark staining zone in center of nucleus
• Site of ribosome and RNA synthesis
39
iv. Chromatin
(structure and function)
• Complex of long thin strands of DNA(de oxyribonucleic
acid) and proteins
• Contains instructions that control cell metabolism and
hereditary
40
CELLULAR ORGANELLES
• Little organs
• Specialized sub-unit that exist within the
cells
• have special functions
• organelles within a cell depends upon the
type of cell under consideration
• May look different depending upon the type
of cell
1
Organelles
Group of small structures that remain
suspended in the cytoplasm and perform
variety of functions
2
ENDOPLASMIC RETICULUM
• a sac-like structure , that remain bound by a
membrane.
• composed of an interconnected network
of flattened sacs or tubes, known as
Cisternae.
• Connects to nuclear envelope and cell
membrane
• Helps in movement of materials with in the
cell.
• Functions as manufacturing unit
• Two types
– Rough endoplasmic reticulum
– Smooth endoplasmic reticulum
3
Rough endoplasmic reticulum
• Has ribosomes attached to the surface
• Makes membrane proteins and proteins
for export out of the cell
4
Smooth endoplasmic reticulum
• Lacks ribosomes
• Attached to the surface of rough
endoplasmic reticulum
• Makes cell products that are used inside
the cell
i. Makes membrane lipids
ii. Destroy toxic substances
iii. Regulates calcium
5
RIBOSOME
• found either floating in the cytosol, or
attached to the membrane of the rough
endoplasmic reticulum.
• Ribosomes are the site of protein
synthesis, also known as Protein Factory
Of Cell
6
GOLGI COMPLEX
• The Golgi apparatus is composed of
'cisternae'.
• It is also responsible for transporting
lipids within the cell.
• Helps in the synthesis of pant cell
wall
7
Mode of action:
• The Golgi apparatus receives proteins
from endoplasmic reticulum, which are
then packaged and transported to various
locations within or outside the cell.
8
9
VACUOLES
• small membrane bound sacs present in the
cytoplasm
– Examples
• Golgi and ER transport vesicles
• Peroxisome
– Where fatty acids are metabolized
– Where hydrogen peroxide is detoxified
• Lysosome
– contains digestive enzymes
– Digests unwanted cell parts and other wastes
10
LYSOSOME
• The lysosome is an example of an organelle
made at the Golgi apparatus.
– Golgi packages digestive enzymes in a vesicle.
The vesicle remains in the cell and:
• Digests unwanted or damaged cell parts
• Merges with food vacuoles and digest the contents
12
• It is
termMedIaTsOthCeH'pOowNeDr
hRoIuAse' of a cell.
• It is a double membrane-bound
structure that can be found in most
eukaryotic cells.
• The function of this organelle is to
produce and store energy in the form
of adenosine triphosphate (ATP)
molecules, which are generated by
using the chemical energy derived 13
structur
e
– ~1-5 microns
– Two membranes
• Outer membrane
• Inner membrane - Highly folded
– Folds called cristae
– Intermembrane space (or outer
compartment)
– Matrix
• DNA and ribosomes in
matrix
14
15
Centrioles
• Pairs of microtubular
structures
• Play a role in cell
division
16
Plant Cell Structures
• Structures found in plant, but not
animal cells
– Chloroplasts
– Central vacuole
– Other plastids/vacuoles – chromoplast,
amyloplast
– Cell wall
Chloroplasts
• Function – site of photosynthesis
• Structure
– 2 outer membranes
– Thylakoid membrane system
• Stacked membrane sacs called granum
– Chlorophyll in granum
– Stroma
• Fluid part of chloroplast
Plastids/Vacuoles in Plants
• Chromoplasts – contain colored
pigments
• Pigments called carotenoids
• Structure
– Wraps around the plasma membrane
– Made of cellulose and other polysaccharides
– Connect by plasmodesmata (channels through the
walls)
Plant Cell TEM
Typical Plant Cell
Typical Plant Cell –add the labels
Origin of Mitochondria and
Chloroplasts
• Both organelles are believed to have
once been free-living bacteria that
were engulfed by a larger cell.
Proposed Origin of Mitochondria
and Chloroplasts
• Evidence:
– Each have their own DNA
– Their ribosomes resemble bacterial
ribosomes
– Each can divide on its own
– Mitochondria are same size as
bacteria
– Each have more than one
membrane
Review of Eukaryotic Cells
Review of Eukaryotic Cells