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Introduction To Laboratory

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7 views

Introduction To Laboratory

Uploaded by

bodurr571
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PPTX, PDF, TXT or read online on Scribd
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Introduction to Laboratory

30.03.2023
Çağla Ece Olgun
B57, [email protected]
Pipettes

Pipette tips
Pipettes

Electronic pipette pomps

Serological pipettes

Manual pipette pomps


Pipettes
Laboratory equipments

Graduated cylinder: Erlenmayer flask Beaker


• To measure the volumes correctly. • Used in microbiology to grow organism. • A volumetric flask with a wide range of usage
• Also called as measuring cylinder. • It is good for the aeration of the area.
microorganisms. • Used to stir, heat (if glass), measure the liquid
• Used to heat and store solutions volume and mix the reagents
• Also called as measuring cylinder.
Laboratory equipments

Petri Dishes Microtubes Falcons


• Also called as petri plate or cell-culture • single-use tubes made from polypropylene • Polypropylene tubes that can be 15 or 50 mls.
dish. for preparing, mixing, centrifuging, • Preperation of buffers, solution storage,
• It is used in biology widely to grow transporting and storing solid and liquid centrifugation, etc.
different organisms or cells such as samples and reagents.
plants, mammalian cells in cell culture or • Also called as eppendorf tubes.
bacteria/yeast.
Laboratory equipments

Centrifuge Bunsen Burner Autoclave


• An apparatus that rotates at high speed • A common piece of laboratory equipment • A pressure chamber to sterilize equipment
and separates substances of different that produces single open gas flame, which is and supplies by subjecting them to high
densities. used for sterilization and heating. pressure saturated steam at 121oC for
• It should be used in balance; otherwise, • Important for aseptic techniques. around 15-20 min depending on the size of
it might explode. the load and the contents.
• It is used to sterilize culture media, discarded
biological waste and other equipments.
Aseptic Techniques
• Aseptic techniques are the precautionary measures taken in order to
prevent contamination of pure cultures and sterile laboratory
equipment.
• The term “aseptic” literally means “without contamination.” These
procedures are as important for the experimenter’s safety as they are for
maintaining culture purity.
• Sterile: Devoid of all life forms (bacteria, fungi,viruses)
• This is all or none.
• Aseptic techniques includes:
• cleaning the bench top work areas with disinfectant solution,
• washing hands with alcohol before starting work ,
• work near the flame,
• use sterile media and glassware.

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