Respiration

Organisms can be classified based on how

they obtain energy:
autotrophs: are able to produce their own
organic molecules through photosynthesis
heterotrophs: live on organic compounds
produced by other organisms
All organisms use cellular respiration to
extract energy from organic molecules.
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 Respiration
Cellular respiration is a series of reactions
that:
-are oxidations – loss of electrons
-are also dehydrogenations – lost
electrons are accompanied by hydrogen

Therefore, what is actually lost is a hydrogen

atom (1 electron, 1 proton).
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 Respiration
During redox reactions, electrons carry
energy from one molecule to another.

NAD+ is an electron carrier.

-NAD accepts 2 electrons and 1 proton to
become NADH
-the reaction is reversible

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 Respiration
During respiration, electrons are shuttled
through electron carriers to a final electron
acceptor.
aerobic respiration: final electron receptor
is oxygen (O2)
anaerobic respiration: final electron
acceptor is an inorganic molecule (not O2)
fermentation: final electron acceptor is an
organic molecule
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 Respiration
Aerobic respiration:

C6H12O6 + 6O2 6CO2 + 6H2O

G = -686kcal/mol of glucose
G can be even higher than this in a cell
This large amount of energy must be
released in small steps rather than all at
once.
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 Respiration
The goal of respiration is to produce ATP.
-energy is released from oxidation reaction
in the form of electrons
-electrons are shuttled by electron carriers
(e.g. NAD+) to an electron transport
chain
-electron energy is converted to ATP at the
electron transport chain
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 Oxidation of Glucose
Cells are able to make ATP via:
1. substrate-level phosphorylation –
transferring a phosphate directly to ADP
from another molecule
2. oxidative phosphorylation – use of ATP
synthase and energy derived from a
proton (H+) gradient to make ATP

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 Oxidation of Glucose
The complete oxidation of glucose proceeds
in stages:
1. glycolysis
2. pyruvate oxidation
3. Krebs cycle
4. electron transport chain & chemiosmosis

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 Glycolysis
Glycolysis converts glucose to pyruvate.
-a 10-step biochemical pathway
-occurs in the cytoplasm
-2 molecules of pyruvate are formed
-net production of 2 ATP molecules by
substrate-level phosphorylation
-2 NADH produced by the reduction of NAD+

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 Glycolysis
For glycolysis to continue, NADH must be
recycled to NAD+ by either:

1. aerobic respiration – occurs when oxygen

is available as the final electron acceptor

2. fermentation – occurs when oxygen is not

available; an organic molecule is the final
electron acceptor
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 Glycolysis
The fate of pyruvate depends on oxygen
availability.

When oxygen is present, pyruvate is

oxidized to acetyl-CoA which enters the
Krebs cycle
Without oxygen, pyruvate is reduced in
order to oxidize NADH back to NAD+
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 Pyruvate Oxidation
In the presence of oxygen, pyruvate is
oxidized.
-occurs in the mitochondria in eukaryotes
-occurs at the plasma membrane in
prokaryotes
-in mitochondria, a multienzyme complex
called pyruvate dehydrogenase
catalyzes the reaction
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 Pyruvate Oxidation
The products of pyruvate oxidation include:

-1 CO2
-1 NADH
-1 acetyl-CoA which consists of 2 carbons
from pyruvate attached to coenzyme A

Acetyl-CoA proceeds to the Krebs cycle.

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 Krebs Cycle
The Krebs cycle oxidizes the acetyl group
from pyruvate.
-occurs in the matrix of the mitochondria
-biochemical pathway of 9 steps
-first step:
acetyl group + oxaloacetate citrate
(2 carbons) (4 carbons) (6 carbons)

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 Krebs Cycle
The remaining steps of the Krebs cycle:
-release 2 molecules of CO2
-reduce 3 NAD+ to 3 NADH
-reduce 1 FAD (electron carrier) to FADH2
-produce 1 ATP
-regenerate oxaloacetate

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 Krebs Cycle
After glycolysis, pyruvate oxidation, and the
Krebs cycle, glucose has been oxidized to:
- 6 CO2
- 4 ATP
- 10 NADH These electron carriers proceed
- 2 FADH2 to the electron transport chain.

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 Electron Transport Chain
The electron transport chain (ETC) is a
series of membrane-bound electron
carriers.
-embedded in the mitochondrial inner
membrane
-electrons from NADH and FADH2 are
transferred to complexes of the ETC
-each complex transfers the electrons to the
next complex in the chain
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 Electron Transport Chain
As the electrons are transferred, some
electron energy is lost with each transfer.

This energy is used to pump protons (H+)

across the membrane from the matrix to
the inner membrane space.

A proton gradient is established.

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 Electron Transport Chain
The higher negative charge in the matrix
attracts the protons (H+) back from the
intermembrane space to the matrix.

The accumulation of protons in the

intermembrane space drives protons into
the matrix via diffusion.

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 Electron Transport Chain
Most protons move back to the matrix
through ATP synthase.

ATP synthase is a membrane-bound

enzyme that uses the energy of the proton
gradient to synthesize ATP from ADP + Pi.

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 Energy Yield of Respiration
theoretical energy yields
- 38 ATP per glucose for bacteria
- 36 ATP per glucose for eukaryotes

actual energy yield

- 30 ATP per glucose for eukaryotes
- reduced yield is due to “leaky” inner
membrane and use of the proton gradient
for purposes other than ATP synthesis
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 Regulation of Respiration
Regulation of aerobic respiration is by
feedback inhibition.
-a step within glycolysis is allosterically
inhibited by ATP and by citrate
-high levels of NADH inhibit pyruvate
dehydrogenase
-high levels of ATP inhibit citrate synthetase

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 Oxidation Without O2
Respiration occurs without O2 via either:

1. anaerobic respiration
-use of inorganic molecules (other than
O2) as final electron acceptor
2. fermentation
-use of organic molecules as final electron
acceptor
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 Oxidation Without O2
Anaerobic respiration by methanogens
-methanogens use CO2
-CO2 is reduced to CH4 (methane)

Anaerobic respiration by sulfur bacteria

-inorganic sulphate (SO4) is reduced to
hydrogen sulfide (H2S)
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 Oxidation Without O2
Fermentation reduces organic molecules in
order to regenerate NAD+
1. ethanol fermentation occurs in yeast
-CO2, ethanol, and NAD+ are produced
2. lactic acid fermentation
-occurs in animal cells (especially muscles)
-electrons are transferred from NADH to
pyruvate to produce lactic acid
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Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display.

Alcohol Fermentation in Yeast

Glucose H
G H C OH
2 ADP L
Y CH3
C 2 NAD+ 2 Ethanol
2 ATP O
L
Y 2 NADH
O – S
H
I
C O S C O
C O CO2
CH3
CH3 2 Pyruvate 2 Acetaldehyde

Lactic Acid Fermentation in Muscle Cells

Glucose O–
G C O
2 ADP L
Y H C OH
C CH3
2 ATP O
2 NAD+ 2 Lactate
L
Y
O– S
I 2 NADH
C O
S
C O
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CH3 2 Pyruvate
 Catabolism of Protein & Fat
Catabolism of proteins:
-amino acids undergo deamination to
remove the amino group
-remainder of the amino acid is converted to
a molecule that enters glycolysis or the
Krebs cycle
-for example:
alanine is converted to pyruvate
aspartate is converted to oxaloacetate
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 Catabolism of Protein & Fat
Catabolism of fats:
-fats are broken down to fatty acids and
glycerol
-fatty acids are converted to acetyl groups
by -oxidation

The respiration of a 6-carbon fatty acid

yields 20% more energy than glucose.
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 Evolution of Metabolism
A hypothetical timeline for the evolution of
metabolism:
1. ability to store chemical energy in ATP
2. evolution of glycolysis
3. anaerobic photosynthesis (using H2S)
4. use of H2O in photosynthesis (not H2S)
5. evolution of nitrogen fixation
6. aerobic respiration evolved most recently
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