Development & Evaluation of Anti Fungal Activity of Polyherbal Anti Dandruff

Shampoo
Sanjeeva Kumar Avvari, Likhitha Reddy Ketham*, Lokeswari Mallampalli,
Hemanth Kuresi, Chandrasekhar R, Niranjan Babu Mudduluru

DEPARTMENT OF PHARMACOGNOSY AND PHYTOCHEMISTRY

SEVEN HILLS COLLEGE OF PHARMACY
(AUTONOMOUS)
TIRUPATI-517561,(A.P), INDIA

Presented during
PHARMA VISION 2024, held at VIGNAN UNIVERSITY in
Vadlamudi, Guntur, 522213

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 INTRODUCTION:
 Shampoos are products that clean the hair shaft and scalp of surface oil and grime.

 Because the majority of the constituents in shampoos are chemicals, there have been serious attacks as a

result of the possibility of side effects associated with their use.

 The studies major goal was to replace dangerous systemic components in polyherbal antidandruff shampoo

formulations with a safer natural substance.

 It also attempted to incorporate modern formulation process into a simple composition based on natural
ingredients1.

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 REVIEW OF LITERATURE:
S.NO AUTHOR Title of work Title of VOLUME PAGE NO YEAR
journal
1. Pawan tiwary, Rakshit Formulation and International Volume - 8, 898-909 2023
Ture,Jay Butley, evaluation of poly journal for Issue - 5
Suyag Lanjeka, herbal liquid research
Vaishnavi Nagthane shampoo

2. Swetha Patel. et al., Formulation and Journal for Volume , 1-9 June - 2022
evaluation of poly researching Issue - 2
herbal anti applied sciences
dandruff shampoo and
and its marketed biotechnology
comparison
3. Anuradha.G et.al Formulation and International Volume - 13(2) 365-369
evaluation of journal for June - 2022
herbal anti ayurvedic
dandruff shampoo medicine
4. Khandiagale Sandip.S Formulation and International Volume - 10 36-41 Dec-2022
et.al evaluation of journal for Issue - 12
herbal neem anti ayurveda and
dandruff shampoo pharma research

5. Rashmi .S. pal et.al Preparation and The open Volume - 14 22-27 2020
assessment of poly dermatology
herbal anti journal
dandruff
formulation

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 1. AIM: To Prepare and Evaluate Poly-herbal Anti dandruff Shampoo

2. OBJECTIVES:
• To prepare polyherbal anti dandruff shampoo
• To evaluate poly herbal anti dandruff shampoo by reported methods for
2.1) Visual inspection
2.2) Foaming ability and foam stability
2.3) Determination of pH
2.4) Percent of solid content
2.5) Wetting test
2.6) Stability
2.7) Surface tension
2.8) Dirt dispersion
2.9) In vitro anti fungal activity

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 3.MATERIALS AND METHODS:
3.1 MATERIALS:
Crude drugs: 1) Bringraj 2) Neem 3) Hibiscus 4) Tulasi 5) Aloe-vera 6) Orange 7) Curry leaves 8)
Rice water 9) Soap nut

Equipments: 1) Calibrated pH meter 2) Graduated cylinder 3) Hot plate 4) China dish 5)Stop
watch 6) Stalagnometer 7) Canvas paper 8)Disc

Chemicals: 1) India ink 2) Sebum 3) Citric acid 4) Hexane 5) Distilled water

3.2 METHODS:
3.2.1) Collection, Drying and size reduction of plant material
3.2.2) Design of polyherbal antidandruff shampoo

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 3.2.2 TABLE: FORMULATION OF POLY HERBAL ANTI DANDRUFF
INGREDIENTS SHAMPOO F1 F2
FUNCTIONS F3 F4 F5 F6 F7 F8
BHRINGRAJ Slow down hair greying 2% 3% 1% 4% 3% 2% 1% 3%
NEEM Anti bacterial 4% 3% 2% 3% 1% 4% 2% 1%
HIBISCUS Remove dandruff and 1% 3% 2% 4% 1% 2% 3% 2%
itchiness
TULASI Antifungal 3% 2% 4% 1% 2% 3% 1% 3%
ALOEVERA Remove dead cells 2% 1% 3% 2% 1% 2% 3% 2%
ORANGE fragrance 1% 2% 1% 3% 2% 1% 2% 1%
CURRY LEAVES Moisturize the scalp 3% 1% 2% 2% 3% 2% 1% 3%
RICE WATER Prevent hair fall 2% 3% 1% 3% 1% 1% 3% 2%
SOAPNUT Cleanser 1% 2% 3% 1% 2% 3% 1% 1%
SODIUM LAURYL Detergent 10% 20% 30% - - - 15% 15%
SULPHATE
SODIUM CHLORIDE Thickening agent 3% 3% 3% - - - 1% -
GELATINE Natural gum - - - 10% 20% 30% - -
CITRIC ACID pH adjuster and preservative - - - - - - 0.3% 0.3%
TWEEN 80 Solubilizing agent - - - - - 1% 1% -
6 EDTA Sequestering agent - - - - - 1% 1% -
EVALUATIONS:
1)VISUAL INSPECTION:
The created formulations were assessed based on their fluidity ,clarity and capacity to produce foam.

2) FOAMING ABILITY AND FOAM STABILITY:-

Foaming ability was evaluated using the cylinder shake method.

3ml of shampoo was taken, and 10ml of water was added to dilute it .

Shaken the cylinder vigorously in under a second twice , then leave it alone for 15 uninterrupted minutes.

After shaking ,the total volume of foam content was measured.

Only the foam volume was computed.

The amount od foam procedure immediately after shaking was measured 4 times ,every one minute.

The foam volume required constant over the course of around 5 minutes, demonstrating the shampoo's foam generation
process procedures foam that is both stable and exhibits higher foam properties possibly as a result of soap nut.

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3.DETERMINATION OF PH:
The pH of 10%v/v shampoo solution in distilled water was measured by using calibrated pH.

4.PERCENT OF SOLID CONTENT:

Weighing a piece of dry clean China dish

We add 4ml of shampoo

The shampoo filled dish was weighed

It was computed how much shampoo exactly weighed

The shampoo filled porcelain dish was placed on the hot plate and left there until the liquid evaporated

After drying the weight was calculated

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5.WETTING TIME :-
The length of time the canvas paper needed to soak up all the water was determined as the wetting time. A disc with
a 1-inch diameter was made from a piece of canvas paper weighing 0.44g .The canvas paper disc was placed over the
shampoo (1%v/v) surface ,and the amount of time it took for the paper to sink was calculated using a stopwatch.

6.STABILITY STUDY:-
The stability of the formulation was studied for a period of 4 weeks by keeping at
temperatures of 25°-30°C.The shampoo is free from microbial
contamination and remains stable at room temperature.

7.SURFACE TENSION:-
The cleaned stalagmometer was filled to mark A with the prepared shampoo that would be tested.

The amount of drops of that the liquid will produce as it travels from mark A to mark B was then computed once it should
to rain owing to gravity.

To determine the mean value ,the process was performed three times.

The surface tension can be determined by using the formula.

V1=n2ρ1/n1ρ2xV2

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 

8. DIRT DISPERSION:
Two drops of shampoo was added to 10 ml of water containing test tube and one drop
of ink was added in test tube.
Shake test tube for 10 min. The amount of ink in foam was estimated as None, Light, Moderate or Heavy indicates the dispersion nature
of shampoo.

ANTI-FUNGAL ACTIVITY
The Antifungal activity was checked by following Zone Inhibition Method (Kirby-Bauer method).
The SDA plates were inoculated by spreading with 100 µl of Fungal culture, C. albicans (adjusted to 0.5 McFarland Unit - Approx cell
density (1.5 X 108 CFU/mL) and followed by placing the discs containing 10 µl of different concentration (0 to 100 mg/ml).
One disc in each plate was loaded with solvent alone which served as vehicle control and Amphotericin B disc (50 µg) was taken as
positive control.
The plates of C. albicans were incubated (Basil Scientific Corp. India- Incubator) at 37 °C for 24 hrs. The clear zones created around the
disc were measured and recorded.

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 RESULTS AND DISCUSSION:

Characters of Poly-herbal Anti-Dandruff Shampoo

Percentage Yield
S.NO Name of the extract Colour Consistency
(W/W)

1. Neem leaves extract Green Semisolid 2.2

2. Tulasi leaves extract Green Semisolid 2.9

3. Bhringraj leaves extract Green Semisolid 2.9

4, Curry leaves extract Green Semisolid 2.3

5. Hibiscus flowers extract Red Semisolid 2.8

6. Soap nut fruit extract Brown Semisolid 2.4

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EVALUATION OF PREPARED POLY HERBAL FORMULATION:

S. Evaluation
parameters F1 F2 F3 F4 F5 F6 F7 F8
No.
Dark Dark Dark
1. Visual Inspection Brick red Dark brown Dark brown Light brown Brick red
brown brown brown

Foaming ability&
2. Foam stability 110 ml 150 ml 140 ml 100 ml 130 ml 120 ml 100 ml 150 ml

Determination of
3. pH 6.54 7.74 7.67 6.32 5.86 7.57 6.25 7.43

Percentage of Solid
4. content 25% 26% 21.7% 23.8% 24% 25.3% 22.5% 21%

5. Wetting Test 35 sec 40 sec 45 sec 90 sec 80 sec 85 sec 55 sec 50 sec

6. Stability Test Stable Stable Stable Stable Stable Stable Stable Stable

7. Surface tension 32.15 33.22 31.37 34.60 33.61 35.43 33.23 31.23

8. Dirt dispersion Moderate Moderate Light Moderate Light Light Moderate Light
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 ANTIFUNGAL ACTIVITY OF POLY HERBAL ANTIDANDRUFF SHAMPOO ON
C.ALBICANS
Amount Plate A Plate B Plate C
Average SD SEM
(µg/Disc) (mm) (mm) (mm)
PC* 15 15 16 15.33333 0.57735 0.408248
0 0 0 0 0 0 0
50 9 9 10 9.333333 0.57735 0.408248
125 8 8 10 8.666667 1.154701 0.816497
250 10 10 8 9.333333 1.154701 0.816497
500 10 11 11 10.66667 0.57735 0.408248
1000 14 14 14 14 0 0

*PC= Nystatin (10 µg/disc)

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 Summary and Conclusions
Based on the results obtained from the study, test organism was treated with
different amount of sample on agar plate, and Maximum zone of inhibition
(ZOI) created around the treatment disc was estimated (Diameter) for Sample
(14.00 mm at 1000 µg dose) against the test organism C. albicans as compared
to positive control (zone of Inhibition 15.33 mm at 10 µg dose of Nystatin).
From this work, it can be concluded that the formulated polyherbal shampoo
possess anti fungal activity.

Acknowledgements
Authors express their heartfelt thanks to the management and staff of Seven
Hills College of Pharmacy (Autonomous), Tirupati for providing necessary
facilities to conduct the research.

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 THANK YOU

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