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202 DNA Switch

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3 views

202 DNA Switch

Uploaded by

shazia
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd
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Biosensors

202. DNA based


Molecular Switch
Biosensors
DNA based Molecular switch Biosensors
Introduction
1. DNA-based molecular
switchws are devices.
2. They are highly specific
sensors, diagnostic tools.
3. DNA molecular switches, a
basic class of DNA
nanostructures, turn some
process
 on
 off
depending on whether a
substrate binds to the switch..
DNA based Molecular switche Biosensor
Principle
In Biosensor technology the
principle of molecular switching is
used and are known as molecular
beacons.
1. Single stranded DNA
molecule is used as a probe.
2. A fluorescent dye
3. A quencher in close
proximity.
incoming light will not result in
a visible emission due to
quenching.
DNA based Molecular switche Biosensor
Moleculr Beacon
.

https://www.sigmaaldrich.com/content/dam/sigma-aldrich/
life-science/custom-dna-probes/molecular-beacons.jpg
DNA based Molecular switche Biosensor
Hydrodynamic DNA switch
1. Hydrodynamic DNA switch
detct the target molecule by
change in the oscillating
movement of DNA probe.
2. It detects the size /shape of
analyte.
3. When analytes bind to
oscillating DNA nanolevers on
the movement is slowed by the
additional friction imposed by
the analyte.
4. hereby revealing its size or
shape change.
DNA based Molecular switche Biosensor
Hydrodynamic DNA switch

The fluorescence proximity sensing mode detects the binding of


molecules in real-time through changes in the dye`s local environment.
https://www.dynamic-biosensors.com/wpcms/wp-content/uploads/2019/10/
switchSENSE_dynamic-switching-motion.jpg
DNA based Molecular switche Biosensor
Three way Junction DNA
switch
A series of three-way junction
DNA-based molecular
switches. It has two domains
1. Recognition domain which
interacts with the target of
interest and induces a
structural change in the
TWJ,
2. Transduction domain, which
https://blogs.rsc.org/sc/files/2019/11/DNA- then becomes accessible and
Switches-Fig-1-226x300.gif binds to a fluorescent
molecule.
DNA based Molecular switch Biosensor

Three way Junction DNA switch


.

https://blogs.rsc.org/sc/files/2019/11/DNA-Switches-Fig-2.gif
DNA based Molecular switch Biosensor
Related Questions
1. What is a DNA molecular switch.
2. Give different types of molecular
switches.
3. Give the principle used in the
fabrication of molecular switches in
Biosensor Technology.
4. Elaborate the design of a molecular
beacon.
5. What is hydrodynamic switch
technology.

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