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5.paper Chromatography

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8 views

5.paper Chromatography

Uploaded by

sitataleen
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd
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Chemistry

Sharjah International Private School

Sharjah International Private School


Objectives and Success
Criteria Sc1:
Title: Separation
SC2:
Techniques SC3:I can analyse and interpret which
separation technique to use depending
Learning Objective : on the nature of components
1.11 understand how a chromatogram
provides information about the
composition of a mixture 1.12
understand how to use the calculation
of Rf values to identify the
components of a mixture 1.13
practical: investigate paper
chromatography using inks/food
colourings

Sharjah International Private School


Entry Ticket/ Starter
Activity:

Sharjah International Private School


Starter
Separation Techniques

The following are ways to separate mixtures:

• Filtration

• Crystallization

• Distillation

• Paper Chromatography

Sharjah International Private School


Filtration

Observe what’s
happening here!

Think

Share

Sharjah International Private School


Paper Chromatography
• This technique is used to separate substances that have different solubilities
in a given solvent (e.g., different colored inks that have been mixed to make
black ink)

• A pencil line is drawn on chromatography paper and spots of the sample


are placed on it.

• Pencil is used for this as ink would run into the chromatogram along with the
samples.

• The paper is then lowered into the solvent container, making sure that the
pencil line sits above the level of the solvent, so the samples donʼt wash into the
solvent container.

• The paper is called the stationary phase.

• The solvent travels up the paper by capillary action, taking some of the
coloured substances with it; it is called the mobile phase. Sharjah International Private School
Paper Chromatography
• Different substances have different solubilities so will travel at
different rates, causing the substances to spread apart.

• Those substances with higher solubility will travel further than the
others. This will show the different components of the ink / dye.

• If two or more substances are the same, they will produce identical
chromatograms.

• If the substance is a mixture, it will separate on the paper to show all


the different components as separate spots.

• An impure substance will show up with more than one spot.

• A pure substance should only show up with one spot separated.

Sharjah International Private School


Paper Chromatography

Sharjah International Private School

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