Scribd
Upload
2 views

Unit 6 Matabolism

The document outlines exercises related to photosynthesis and respiration, including the uptake of carbon dioxide (CO2) during photosynthesis and the detection of starch using iodine. It describes the role of chlorophyll and dehydrogenase enzymes in these processes, as well as an experiment demonstrating CO2 production during anaerobic respiration (fermentation) using yeast. Various methods and materials are detailed for conducting these experiments, including the use of phenol red, methylene blue, and balloons to capture CO2.

Uploaded by

Processpage8744
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd
2 views

Unit 6 Matabolism

The document outlines exercises related to photosynthesis and respiration, including the uptake of carbon dioxide (CO2) during photosynthesis and the detection of starch using iodine. It describes the role of chlorophyll and dehydrogenase enzymes in these processes, as well as an experiment demonstrating CO2 production during anaerobic respiration (fermentation) using yeast. Various methods and materials are detailed for conducting these experiments, including the use of phenol red, methylene blue, and balloons to capture CO2.

Uploaded by

Processpage8744
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd
You are on page 1/ 11

UNIT SIX

Metabolism
(Photosynthesis and
Respiration)
EXERCISE 1:
Uptake of Carbon Dioxide (CO2) during
photosynthesis
• Phenol red (phenol-sulfonphthalin) is
a pH-indicator that
• turns yellow in an acidic solution
(pH< 7) and becomes red in a
neutral to basic solution (pH> 7).
.
Carbon dioxide in your breath will
dissolve in water to form carbonic
acid, which lowers the pH of the
solution producing a yellow color

Carbon
dioxide
make water
acidic
• As the plant utilizes CO2, the pH rises. When
the pH rises above 7, the solution will
become red again.

Place tube approximately


0.5 m in front of a 100-watt
bulb for 30-60 min.
EXERCISE 2:
The use of light and chlorophyll in
reducing starch formation during
photosynthesis
• This exercise used to detect the presence of
starch by staining it with a solution of iodine.

Chlorophyll
is insoluble
in water but
it is soluble
in methanol
or ethanol.
EXERCISE 4:
Dehydrogenase in yeast

During respiration, hydrogen atoms are removed from glucose


molecules by enzymes called dehydrogenases and passed to
various chemicals called hydrogen acceptors.

As the hydrogen atoms pass from one hydrogen acceptor to


another, energy is made available for chemical reactions in the
cell.
In this experiment, a dye called methylene blue acts as an
artificial hydrogen acceptor.
When this dye is reduced by accepting hydrogen atoms it
goes colorless
• Place yeast suspension in a test-tube, heat
this suspension over a Bunsen flame until
the liquid boils. Then cool the tube under
the tap ( boiled yeast )
• Label three test-tubes 1-3
Tube 1 Boiled yeast + water

Tube 2 unboiled yeast +water


Tube 3 unboiled yeast +
water + glucose

• Place all three tubes in water


bath with temperature of 35-450C
• After 5 minutes draw up methylene
blue solution in each tube.

• Shake all three tubes thoroughly and return them to the water bath
EXERCISE 5:
Production of CO2 during anaerobic respiration
(fermentation)
1. Label the Erlenmeyer flasks 1, 2, 3
Flask
Water + Sugar
1
Flask
Water + Yeast
2
Flask
2. Put the Water
pre-stretched balloon
+ Yeast + Sugar
3
immediately over the top of the
flask
3. Allow the flasks to sit aside
for 30 minutes.
The balloon is actually filling up
with carbon dioxide

You might also like