Continous Fermentation (1)
Continous Fermentation (1)
C H E M O S TAT T U R B I D O S TAT
8. Supply of a single nutrient controls the growth 8. Supply of a single nutrient does not control the
rate of the microbe. growth rate of the microbes.
A D VA N TA G E O F C O N T I N O U S C U LT U R E
There are several advantages to continuous culture, where all the problems associated with the
batch culture are solved.
Firstly, the growth rate is controlled and the cells are well maintained, since fresh media is
replaced by old culture while the dilution is taking place. As a result, the effect of physical and
chemical parameters on growth and product forma- tion can easily be examined.
The biomass concentration in the cultured broth is well main- tained at a constant dilution rate.
The continuous process results in substrate-limited growth and cell-growth-limiting nutrients.
The composition of the medium can be optimised for maximum productivity; in addition
secondary metabolite production can also be controlled.
The growth kinetics and kinetic constants are accurately determined. The process leads to
reproducible results and reliable data. High productivity per unit volume is achieved.
The continuous culture is less labour-intensive, and less downtime is needed. Finally, steady-
state growth can be achieved, even if mixed cultures are implemented.
C O M P A R I S O N I N B AT C H C U LT U R E A N D C O N T I N O U S
C U LT U R E .
B AT C H F E R M E N TAT I O N C O N T I N O U S F E R M E N TAT I O N
3) Simple easy to sterilize ,minimal monitoring 3) Complex , requires automation ,strict controls .
4) Contamination risk higher
4) Contamination risk lower
5) Higher capital equipment,lower labour .
5) Lower capital ,higher labour per batch
6) Application in large scaleindustry low value products like bio
6) Application in small scale ,high value products pharma etc fules.
7) All phases , lag,exponential, stationary ,death. 7) Microbes maintained in exponential phase steady state growth
8) All nutrient added at the start . 8) Nutrient continously fed into the reactor.
9) Genetic stability is stable , short cycles minimize the 9) Risk of genetic drift mutation , longterm growth under stable
adaptation . conditions .
10) Discrete wastes batches ,easier management 10) Continous waste stream requires steady treatment.
11) Bio ethanol,waste water treatment , singlecell protine ,yeast
11) Example beer ,wine antibiotics ,speciality
biomass.
T H A N K YO U
3rd year