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Practical 2 Microscopy

The document provides a detailed overview of the parts and functions of a light microscope, including ocular lenses, adjustment knobs, objective lenses, and other components. It also outlines procedures for examining living microorganisms, such as making wet mount slides and observing pond water and baker's yeast under various magnifications. Additionally, it includes links to resources for further study and observation results of microorganisms.

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1 views

Practical 2 Microscopy

The document provides a detailed overview of the parts and functions of a light microscope, including ocular lenses, adjustment knobs, objective lenses, and other components. It also outlines procedures for examining living microorganisms, such as making wet mount slides and observing pond water and baker's yeast under various magnifications. Additionally, it includes links to resources for further study and observation results of microorganisms.

Uploaded by

masabalalalondi
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd
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MKBN 211

The Light
Microscope

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Parts of the Light Microscope
• 1. Ocular lenses: The part you look through, has a
magnification of 10X.

• 2. Coarse Adjustment Knob: Usually the larger of


the two knobs; use this first to bring the object into
rough focus.
• Use only under low and medium power!!!!

• 3. Fine Adjustment Knob: Smaller of the two knobs;


used to bring the object into “perfect” focus. (always
use this last)

• 4. Arm: used to carry the microscope.


Parts of the Light Microscope

• 5. Rotating Nose piece: The knob that moves the


objective lens into place.

• 6. High Power Objective Lens: Largest


objective lens, has a magnification power of
40X; always used last in focusing.

• 7. Medium Power Objective Lens: Middle objective


lens in terms of size; has a magnification power of
10X. (Use this after the low power has been focused)

• 8. Low-Power Objective Lens: Shortest Objective


lens; has a power of 4X. (Use this first when focusing on
the object)
Parts of the Light Microscope

• 9. Stage: Place where the slide is placed.


• 10. Stage Clips: Holds the slide in place.
• 11. Diaphragm: A disc that regulates the amount of
light hitting the object.
• 12. Base: Bottom of the microscope; used to carry
the microscope with an arm.
• 13. Light/Mirror: Illumination source for the microscope.
(Provides light to view the object)
• 14. Revolving Nose piece: Holds and turns the
objectives into viewing position.
• 15. Body Tube (Eyepiece Tube): Connects the
eyepiece to the revolving nosepiece.
I – Examination of living microorganisms

Place a drop of the pond water on the


EXPERIMENT 3.2: microscope slide
INTRODUCTION
INTO MICROSCOPY
Cover with the coverslide

Observe the slides under the microscope


under various magnifications (BUT NOT
100 x objective)
Staining:
Wet
mount
• Making a Wet Mount Slide

• Carefully place the item you want to look at in the


center of a clean, glass slide. Make sure the sample is
thin enough for light to pass through it.

• Use a dropper to place one or two drops of pond water


on the sample.
Making a

• Hold a clean cover slip by the edges and place it at one


Mount

edge of the water. Slowly lower the coverslip (45


degree angle) onto the water until it lies flat.
Slide
Wet

• If you have too much water or a lot of air bubbles,


touch the edge of a paper towel to the edge of the
coverslip to draw off extra water and draw out
unwanted air.
INTRODUCTI
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MICROSCOP
Y
Observation Results:
MICROSCOPY
I – Studying of pond water

https://
www.youtube.com/watch?v=XAXDSDkZmx
c

https://
www.youtube.com/watch?v=7JwVfAldL2o
Bacteria

Observations:
Microscopy
Ciliates Diatoms
Diatoms

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5/a-transparent-sphere-with-small-moving-
green-dots

Algae

https://merismo.tumblr.com/post/57394407655/a-transparent-sphere-with-small-moving-green-dots
II – Studying of bakers yeast
(Saccharomyces cerevisiae)

Place a drop of the yeast solution on the


microscope slide
INTRODUCTION
INTO
MICROSCOPY Cover with the coverslide

Observe the slides under the microscope


under various magnifications (BUT NOT
100 x objective)
Observation: MICROSCOPY

II – Studying of bakers yeast (Saccharomyces


cerevisiae)

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