Scribd
Upload
213 views17 pages

1. Ppt Enzyme Immobilization

The document outlines various methods of enzyme immobilization, including adsorption, cross-linking, covalent bonding, entrapment, and encapsulation. Each method has distinct mechanisms and applications, with immobilized enzymes being utilized across multiple industries such as medical, food, and pharmaceuticals. The choice of immobilization technique depends on the specific application and required carrier materials.

Uploaded by

zeealigaming
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd
213 views17 pages

1. Ppt Enzyme Immobilization

The document outlines various methods of enzyme immobilization, including adsorption, cross-linking, covalent bonding, entrapment, and encapsulation. Each method has distinct mechanisms and applications, with immobilized enzymes being utilized across multiple industries such as medical, food, and pharmaceuticals. The choice of immobilization technique depends on the specific application and required carrier materials.

Uploaded by

zeealigaming
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd
You are on page 1/ 17

METHODS OF ENZYMES

IMMOBILIZATION
Methods
Adsorption
Cross Linking
Entrapment
Covalent Bonding
Encapsulation
Adsorption
• Physical adsorption is a method used for the
immobilization of enzymes. It involves the reversible
attachment of enzymes onto a solid support through weak
non-specific forces such as van der Waals forces,
hydrogen bonds, and hydrophobic interactions.
Cross-Linking Method
The cross-linking method for immobilizing enzymes
involves the use of bi-functional or multi-functional cross-
linking reagents to form stable bonds between enzyme
molecules, creating aggregates known as cross-linked
enzyme aggregates (CLEAs. Cross linking agent such as
Glutaraldehyde is added to enzyme solution
Glutaraldehyde is a bifunctional reagent that can react with
amino acids groups on the enzyme molecules forming
covalent bond. The enzyme solution with the cross linking
agent is incubated at an optimal temperature and pH to
allows the cross linking reaction occur.
Covalent Bonding
The covalent bonding method for the
immobilization of enzymes involves
attaching enzymes to an insoluble support,
such as silica gel or polymer beads, through
a covalent bond. This method is known for
providing a strong interaction between the
enzyme and the support, resulting in
minimal leakage of the enzyme during
catalysis.
Entrapment
• The entrapment method for the formation of
immobilized enzymes involves trapping
enzymes within a matrix or network, such
as a gel, which restricts their movement but
allows the substrate to reach them.
• The matrices used for entrapping of
enzymes include polyacrylamide gel,
collagen, gelatin, starch, cellulose, silicone
and rubber.
Encapsulation
• It is type of entrapment technique used to
encapsulate enzymes within tiny, spherical
semipermeable membrane.
• The matrix encapsulation is the ideal
process for encapsulating valuable
substances such as oils or active
components in a suitable material. In this
process, different substances are
embedded in a base material by means of
the fluidized bed technology.
Applications
• The immobilized enzymes can be utilized in
various large-scale industries,
e.g., medical, food, detergent, textile, and
pharmaceutical industries, besides being
used in water treatment plants. According
to the required application, a suitable
enzyme immobilization technique and
suitable carrier materials are chosen.

You might also like