Biomedical Instrumentation - Chapter 2
Biomedical Instrumentation - Chapter 2
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Biopotential Electrodes
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Biopotential Electrodes
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Biopotential Electrodes
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Biopotential Electrodes
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Biopotential Electrodes
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Biopotential Electrodes
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Biopotential Electrodes
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Biopotential Electrodes
The resistance-capacitance
networks represent the
impedance of the electrodes as
fixed values of resistance and
capacitance.
The impedance is not constant.
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Biopotential Electrodes
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Biopotential Electrodes
If the amplifier to which the electrodes are connected has an
extremely high input impedance, the effect of polarization or any
other change in electrode impedance is minimized.
Size and type of electrode are also important in determining the
electrode impedance.
Larger electrodes tend to have lower impedances.
Surface electrodes generally have impedances of 2 to 10kΩ, whereas
small needle electrodes and microelectrodes have much higher
impedances.
Input impedance of the amplifier must be several times that of the
electrodes.
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Classifications
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Microelectrodes
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Microelectrodes
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Metal Microelectrodes
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Micropipet Electrodes
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Micropipet Electrodes
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Micropipet Electrodes
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Supported Glass Micropipette
• Electrodes used to measure ECG, EEG, and EMG potentials from the
surface of the skin.
• Many different types of electrode for recoding various potentials on
the body surface have been developed over many years
• There are 3 main types of the skin surface electrodes
1. Metal-plate electrodes
2. Suction electrodes
3. Floating electrodes
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Limb Electrodes/ Metal Plate Electrodes
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Metal Plate Electrode Used For
Application of Limbs
• It consist of a flat metal plate that has been bent into a cylinder
segment
• A terminal is placed on its outside surface near one end; this terminal
is used to attach the lead wire to the electrocardiograph
• A post placed on the same side near the center is used to connect a
rubber trap to the electrode and hold it in place on arm or leg
• The electrode is traditionally made of Germen silver (A nickel silver
alloy)
• Before it is attached to the body its
concave surface is covered with
electrolyte gel
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Metal Disk Electrode used with
Surgical Tape
• It has lead wire soldered or welded to the back surface
• It can be made of several different materials
• The connection between lead wire and electrode is protected by a
layer insulating material such as epoxy or polyvinylchloride
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Metal Disk Electrode used with
Surgical Tape
• It consist of a relatively large disk of plastic foam material with silver
plated disk. A lead wire is connected to electrode
• Silver plated disk serves as electrode and may coated with AgCl layer
• The electrode side of foam is covered with an adhesive material that
is compatible with the skin
• A protective cover or strip of paper or cotton or cloth placed over the
electrode side
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Suction-cup Electrode
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Floating electrodes
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Needle Electrodes
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Needle Electrodes and Wire
Electrodes
• Needle electrodes uncomfortable for long-term implantations.
• Wire electrodes are used for long term implantation
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Biochemical transducers
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Reference Electrodes
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Reference Electrodes
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Reference Electrodes
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Ag-AgCl electrode
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Calomel Electrode
• An equally popular reference electrode is the calomel electrode.
• Calomel is another name for mercurous chloride, a chemical
combination of mercury and chloride ions.
• The interface between mercury and mercurous chloride generates the
electrode potential.
• By placing the calomel side of the interface in a potassium chloride (KCl)
filling solution, an electrolytic bridge is formed to the sample solution
from which the measurement is to be made.
• Like the Ag-AgCl electrode, the calomel electrode is very stable over
long periods of time and serves well as a reference electrode in many
electrochemical measurements.
• Also, like the Ag-AgCl electrode, the electrode potential of the calomel
electrode depends on the concentration of KCl.
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pH Electrode
• The most important indicator of chemical balance in the body is the pH
of the blood and other body fluids.
• The pH is directly related to the hydrogen ion concentration in a fluid.
• Specifically, it is the logarithm of the reciprocal of the H+ ion
concentration.
• In equation form,
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pH Electrode
• Most human body fluids are slightly basic.
• The pH of normal arterial blood ranges between 7.38 and 7.42
• The pH of venous blood is 7.35, because of the extra CO2
• Because a thin glass membrane allows passage of only hydrogen ions in
the form of H3O+, a glass electrode provides a * 'membrane" interface
for hydrogen.
• The principle is illustrated in Figure 4.15. Inside the glass bulb is a highly
acidic buffer solution.
• Measurement of the potential across the glass interface is achieved by
placing a silver-silver chloride electrode in the solution inside the glass
bulb and a calomel or Ag-AgCl reference electrode in the solution in
which the pH is being measured.
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pH Electrode
• In the measurement of pH and, in fact, any electrochemical
measurement, each of the two electrodes required to obtain the
measurement is called a half-cell.
• The electrode potential for a half-cell is sometimes called the half-cell
potential.
• For pH measurement, the glass electrode with the silver-silver chloride
electrode inside the bulb is considered one half-cell, while the calomel
reference electrode constitutes the other half-cell.
• To facilitate the measurement of the pH of a solution, combination
electrodes of the type shown in Figure 4.16 are available, with both the
pH glass electrode and combination electrodes of the type shown in
Figure 4.16 are available, with both the pH glass electrode and reference
electrode in the same enclosure.
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pH Electrode
• The glass electrode is quite adequate for pH measurements in the
physiological range (around pH 7), but may produce considerable error
at the extremes of the range (near pH of zero or 13 to 14).
• Special types of pH electrodes are available for the extreme ranges.
• Glass electrodes are also subject to some deterioration after prolonged
use but can be restored repeatedly by etching the glass in a 20 percent
ammonium bifluoride solution.
• The type of glass used for the membrane has much to do with the pH
response of the electrode.
• Special hydroscopic glass that readily absorbs water provides the best
pH response.
• Special hydroscopic glass that readily absorbs water provides the best
pH response.
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pH Electrode
• Modern pH electrodes have
impedances ranging from 50 to
500 megohms .
• Thus, the input of the meter
that measures the potential
difference between the glass
electrode and the reference
electrode must have an
extremely high input
impedance.
• Most pH meters employ
electrometer inputs.
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Blood Gas Electrodes
• Among the more important physiological chemical measurements are
the partial pressures of oxygen and carbon dioxide in the blood.
• The partial pressure of a gas is proportional to the quantity of that gas in
the blood.
• The effectiveness of both the respiratory and cardiovascular systems is
reflected in these important parameters.
• The partial pressure of oxygen, PO2, often called oxygen tension, can be
measured both in vitro and in vivo
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Blood Gas Electrodes
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Blood Gas Electrodes
• A fine piece of platinum or some other noble metal wire, embedded in
glass for insulation purposes, with only the tip exposed, is placed in an
electrolyte into which oxygen is allowed to diffuse.
• If a voltage of about 0.7 V is applied between the platinum wire and a
reference electrode (also placed into the electrolyte), with the platinum
wire negative, reduction of the oxygen takes place at the platinum
cathode.
• As a result, an oxidation-reduction current proportional to the partial
pressure of the diffused oxygen can be measured.
• The electrolyte is generally sealed into the chamber that holds the
platinum wire and the reference electrode by means of a membrane
across which the dissolved oxygen can diffuse from the blood
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Blood Gas Electrodes
• The platinum cathode and the reference electrode can be integrated
into a single unit (the Clark electrode).
• This electrode can be placed in a cuvette of blood for in vitro
measurements, or a micro version can be placed at the tip of a catheter
for insertion into various parts of the heart or vascular system for direct
in vivo measurements.
• One of the problems inherent in this method of measuring P02 is the
fact that the reduction process actually removes a finite amount of the
oxygen from the immediate vicinity of the cathode.
• By careful design and use of proper procedures, modern PO2 electrodes
have been able to reduce this potential source of error to a minimum.
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Blood Gas Electrodes
• Another apparent error in PO2 measurement is a gradual reduction of
current with time, almost like the polarization effect described for skin
surface electrodes in Section 4.2.2.
• This effect, generally called aging, has also been minimized in modern
PO2 electrodes.
• The measurement of the partial pressure of carbon dioxide, PCO2makes
use of the fact that there is a linear relationship between the logarithm
of the PCO2 and the pH of a solution
• Since other factors also influence the pH, measurement of PCO2 is
essentially accomplished by surrounding a pH electrode with a
membrane selectively permeable to CO2.
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Blood Gas Electrodes
• A modern, improved type of PCO2 electrode is called the Severinghaus
electrode.
• In this type of electrode, the membrane permeable to the CO2 is made
of Teflon, which is not permeable to other ions that might affect the pH.
• The space between the Teflon and the glass contains a matrix consisting
of thin cellophane, glass wool, or sheer nylon.
• This matrix serves as the support for an aqueous bicarbonate layer into
which the CO2 gas molecules can diffuse.
• One of the difficulties with older types of CO2 electrodes is the length of
time required for the CO2 molecules to diffuse and thus obtain a
reading.
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Blood Gas Electrodes
• The principal advantage of the
Severing haus-type electrode is the
more rapid reading that can be
obtained because of the improved
membrane and bicarbonate layer.
• In some applications, measurements
of P02 and PC02 are combined into a
single electrode that also includes a
common reference half-cell.
• Such a combination electrode is
shown in diagram form in Figure
4.18.
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Specific Ion Electrodes
• Just as the glass electrode provides a semi permeable membrane for the
hydrogen ion in the pH electrode, other materials can be used to form
membranes that are semi permeable to other specific ions.
• In each case, measurement of the ion concentration is accomplished by
measurement of potentials across a membrane that has the correct
degree of permeability to the specific ion to be measured. to the specific
ion to be measured.
• The permeability should be sufficient to permit rapid establishment of
the electrode potential.
• Both liquid and solid membranes are used for specific ions.
• As in the case of the pH electrode, a Ag-AgCl interface is usually
provided on the electrode side of the membrane, and a standard
reference electrode serves as the other half-cell in the solution.
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Specific Ion Electrodes
• Figure 4.19 shows a solid-state electrode of the type used for
measurement of fluoride ions.
• Figure 4.20 shows three specific ion electrodes along with a pH glass
electrode. The sodium electrode in Figure 4.20(a) is commonly used to
determine sodium ion activity in blood and other physiological solutions.
• The cationic electrode (b) is used when studying alkaline metal ions or
enzymes.
• The ammonia electrode (d) is designed for determinations of ammonia
dissolved in aqueous solutions.
• Its most popular application is in determining nitrogen as free ammonia
or total Kjeldahl nitrogen
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Specific Ion Electrodes
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Specific Ion Electrodes
• Figure 4.21 is a diagram showing the construction of a flow-through type
of electrode.
• This is a liquid-membrane, specific-ion electrode. One of the difficulties
encountered in the measurement of specific ions is the effect of other
ions in the solution.
• In cases where more than one type of membrane could be selected for
measurement of a certain ion, the choice of membrane actually used
might well depend on other ions that may be expected.
• In fact, some specific-ion electrodes can be used in measurement of a
given ion only in the absence of certain other ions.
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Specific Ion Electrodes
• For measurement of divalent ions, a liquid membrane is often used for
ion exchange.
• In this case, the exchanger is usually a salt of an organophosphoric acid,
which shows a high degree of specificity to the ion being measured.
• A calcium chloride solution bridges the membrane to the silver-silver
chloride electrode. the silver-silver chloride electrode.
• Electrodes with membranes of solid materials are also used for
measurement of divalent ions.
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Specific Ion Electrodes
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Transducers for biomedical
applications
• Several basic physical variables and the transducers (active or passive)
used to measure them are listed in Table.
• It should be noted that many variables of great interest in biomedical
applications, such as pressure and fluid or gas flow, are not included.
• These and many other variables of interest can be measured, however,
by first converting each of them into one of the variables for which basic
transducers are available.
• Some very ingenious methods have been developed to convert some of
the more elusive quantities for measurement by one of the transducers
described.
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Transducers for biomedical
applications
Physical Variable Type Of Transducer
Piezoelectric
Force (Or Pressure)
Unbonded Strain Gage
Variable Capacitance
Variable Inductance
Displacement
Linear Variable Differential Transducer
Mercury Strain Gage
Surface Strain Strain Gage
Velocity Magnetic Induction
Thermocouple
Temperature
Thermistor
Photovoltaic
Light
Photo Resistive
Magnetic Field Hall Effect
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Force Transducers
• A design element frequently used for the conversion of physical
variables is the force-summing member.
• One possible configuration of this device is shown in Figure 2.12(a). In
this case, the force-summing member is a leaf spring.
• When the spring is bent downward, it exerts an upward-directed force
that is proportional to the displacement of the end of the spring.
• If a force is applied to the end of the spring in a downward direction, the
spring bends until its upward-directed force equals the downward-
directed applied force, or, expressed differently, until the vector sum of
both forces equals zero.
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Force Transducers
• From this it derives its name ** force-summing member.*' In the
configuration shown, the force-summing member can be used to
convert a force into a variable for which transducers are more readily
available.
• The bending of the spring, for example, results in a surface strain that
can be measured by means of bonded strain gauges as shown in Figure
2.12(b).
• The transducers shown in Figure 2.13 utilize this principle.
• The photographs illustrate that force and displacement transducers are
closely related.
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Force Transducers
• Sometimes, the terms isotonic and isometric are used to describe the
characteristics of these transducers.
• Ideally a force transducer would be isometric; that is, it would not yield
(change its dimensions) when a force is applied.
• On the other hand, a displacement transducer would be isotonic and
offer zero or a constant resistance to an applied displacement.
• In reality, almost all transducers combine the characteristics of both
ideal transducer types.
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Force Transducers
• Figure 2.13, for example, shows the same basic transducer type
equipped with two different springs.
• With the long, soft spring shown in the upper photograph, the
transducer assumes the characteristics of an isotonic displacement
transducer.
• With the short, stiff spring shown in the lower photograph, it becomes
an isometric force transducer.
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Force Transducers
• Figure 2.12(c) shows measurement of displacement using a differential
transformer transducer.
• A less frequently used type of displacement transducer is shown in
Figure 2.12(d).
• Here the displacement of a spring is used to modulate the intensity of a
light beam via a mechanical shutter.
• The resulting light intensity is measured by a photo resistive cell.
• In this example, a multiple conversion of variables takes place: force to
displacement, displacement to light intensity, and light intensity to
resistance.
• This principle is actually employed in the commercial transducer shown
in Figure 2.14.
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Force Transducers
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Force Transducers
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Force Transducers
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Transducers for Displacement,
Velocity and
Acceleration
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Transducers for Displacement,
Velocity and
• Acceleration
If any one of the three variables can be measured, it is possible-at least
in principle - to obtain the other two variables by integration or
differentiation.
• Both operations can readily be performed by electronic methods
operating on either analog or digital signals.
• Expressed in the frequency domain, the integration of a signal
corresponds to a low pass filter with a slope of 6 dB/octave, whereas
differentiation corresponds to a high pass filter with the same slope.
• Because the performance of analog circuits is limited by bandwidth and
noise considerations, integration and differentiation of analog signals is
possible only within a limited frequency range.
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Transducers for Displacement,
Velocity and
• Acceleration
Usually, integration poses fewer problems than differentiation.
• It should also be noted that discontinuities in the transducer
characteristic (e.g., the finite resolution of a potentiometric transducer
in which the resistive element is of the wire-wound type) are
• greatly enhanced by the differentiation process.
• Table 2.2 shows that transducers for displacement and velocity are
readily available.
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Transducers for Displacement,
Velocity and
• Acceleration
However, the principles listed for these measurements require that part
of the transducer be attached to the body structure whose
displacement, velocity, or acceleration is to be measured, and that a
reference point be available.
• Since these two conditions cannot always be met in biomedical
applications, indirect methods sometimes have to be used.
• Contactless methods for measuring displacement and velocity, based on
optical or magnetic principles, are occasionally used.
• Magnetic methods usually require that a small magnet or piece of
metal be attached to the body structure.
• Ultrasonic methods are used more frequently.
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Pressure Transducers
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Pressure Transducers
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Pressure Transducers
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Pressure Transducers
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Flow Transducers
• The flow rate of fluids or gases is a very elusive variable and many
different methods have been developed to measure it.
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Transducers with Digital Output
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Transducers with Digital Output
• Although such transducers are very limited in their application, they are
available for measurement of linear or rotary displacement.
• These transducers contain encoding disks or rulers with digital patterns
(see Figure 2.16) photographically etched on glass plates.
• A light source and an array of photo detectors, usually made up of
photos diodes or photo transistors, are used to obtain a digital signal in
parallel format that indicates the position of the encoding plate, and
thereby represents the displacement being measured.
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Transducers with Digital Output
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