FS-301

Introduction
 Analyzer
Introductio
n
 Structure Introduction
Test by original tube , puncture tube to get sample
Convenient and fast touch screen

Buffer
chamber
Reagent
Automatic cleaning of card
mixing cup chamber

Consumables door
Preset emergency
4 projects, 40 samples on Waste card collection to
position and
the machine at the same prevent biological
protective cover
time contamination
 Accessories Introduction
Sample rack (use 2ml blood collection tube)

Instrument

Waste card box

Power cable Liquid pipeline

Deionized water bucket Activator bucket Water liquid bucket

 Parameter Technical parameters
Basic parameters

Product name
setting
Dry Fluorescence Immunoassay
Analyzer
Light source
Excitation
LED
Center wavelength λ0=470nm
spectrum
（ Model ： FS-301 ） Receiving Center wavelength λ1=525nm
Methodology Fluorescence immunoassay spectrum
Detection channel 20 detection channels
Display system 12-inch touch color screen
Sample tube Original blood collection tube Software system Linux operation system
Single sampling Results
40 samples 20,000 results can be stored
amount management
Multi-item test simultaneously, fully Temperature The incubation and reaction zone includes a
automatic operation mode: automatic Control System temperature control system
Work mode shaking of blood collection tube, get
sample by puncture, mix, drop, incubate, Support built-in laser scanner and external
Scan system
detect, discard the card scanner
Printing system External USB printer
Emergency position Include emergency position Communication
USB interface, serial port, Ethernet interface
hardware
Support test reagent Support LIS connection, computer
4 kinds Communication
cards simultaneously connection, external scanner connection,
support
external printer connection
Test speed 80 tests/hour
Power input 3P power port
Size 700×600×500mm(L×W×H)
Weight About 70kg
 Reagent
Precautions
 Reagent packaging(old
Remove the packing)
protective sleeve of Package specification:
the soft rubber 50 persons/bag
plug 100 persons/box
ID chip: 1/box
Buffer: 2 bottles/box

Remove the black plug

 Reagent packaging(new
packing)

Package specification:
25 persons/bag
100 persons/box
ID chip: 1/box The baffle
Buffer: 4 bottles/box spring is
too tight.

Desiccant
Note: don’t insert in the wrong direction. scattering
 Reagent Parameters
Sample Type and Reaction Reference
Test Item Detection Range Clinical Significance
Sample Volume Time Range
0.1~0.5 ng/mL: Local bacterial infection, early bacterial infection
whole blood:75 µL or viral infection; 0.5~2 ng/mL: It is likely to be systemic bacterial
PCT 15 min 0.1~100 ng/mL 0~0.5 ng/mL infection; 2~10 ng/mL: Systemic bacterial infection (sepsis); >10
serum/plasma:50 µL
ng/mL: Severe sepsis or septic shock ；
CRP: CRP: >10 mg/L: Possible bacterial or viral infection ；
whole blood:8.5µL 0~10 mg/L hs-CRP: 1~3 mg/L: Moderate risk of cardiovascular disease ，
hs-CRP + CRP 3 min 0.5~200 mg/L
serum/plasma:5µL hs-CRP: 3~10 mg/L: High risk of cardiovascular disease; >10 mg/L: Acute
0~1 mg/L inflammation; Newborn: >2 mg/L: Acute inflammation.
0.5~1.5 ng/mL: Low risk of thrombosis; 1.5~3 ng/mL: moderate
whole blood:15µL
D-Dimer 3 min 0.1~10 mg/L 0~0.5 mg/L risk; 3~5 ng/mL: High risk; >5 ng/mL: High risk (increased
plasma:10µL
mortality)
Whole bllod:75µL >450 ng/L: It indicates the risk of heart failure, and it should be
NT-proBNP 15 min 18~35000 ng/L 0~300 ng/L
Serum/plasma:75µL judged according to the patient's age and other factors
Whole blood:75µL
cTnI 15 min 0.03~50 ng/mL 0~0.3 ng/mL >0.3ng/mL: Indicating the risk of myocardial infarction
Serum/plasma:75µL
Whole blood:75µL
Myo 15 min 2.4~400 ng/mL 0~58 ng/mL >58ng/mL: Indicating the risk of myocardial infarction
Serum/plasma:50µL
Whole blood:75µL
CK-MB 15 min 0.32~80 ng/mL 0~5 ng/mL >5ng/mL: Indicating the risk of myocardial infarction
Serum/plasma:70µL

cTnI 0.1-50ng/mL
cTnI/Myo/CK- Whole blood:75µL Same as each
15 min CK-MB 0.3-100ng/mL Same as each item
MB Serum/plasma:75µL item
Myo 2-400ng/mL
 Storage Conditions

Reagents that have not been disassembled:

stored at 4-30 ℃, free from moisture, and valid for 24 months.

Reagents loaded with the machine:

It can be stored in the machine for 48 hours. If reagent and buffer are placed
for a long time, the test results will be affected.
The opened reagent can be stored for one month in a sealed bag,
and the buffer solution can be stored for one month when the bottle cap is
tightly closed.
Installation
Procedure
 Installation Procedure

Installation Procedure
Unpackaging and
Communication Layout preparation
check

Simulated test Training Maintenance

A clear and logical procedure always is

the key to succeed
 Installation Procedure

Unpackage
 Installation Procedure

Check
 Installation Procedure

Layout
 Installation Procedure

Preparation
 Installation Procedure

Reagent
（ old ）
 Installation Procedure

Reagent （ new
）
 Installation procedure

Training
 Installation procedure

Add
consumables
 Installation procedure

Edit sample information

 Installation procedure

Query
result
 Operation procedure of QC
2. Click 【 Start 】 button ， apply for QC test
1. 【 Function 】 - 【 QC 】 - 【 QC gather 】 -
Setting ： sample type ： QC - sample amount
【 Add 】
- item type （ QC information ） - QC lot
Setting ： QC lot number- validity time-
number – QC level
item - level （ 1/2/3 ） - target value -
Click 【 confirm 】 - 【 add 】 - 【 start 】， start QC
upper - lower
test
Operation procedure of QC

3. Draw L-J ：【 Function 】 - 【 QC 】 - 【 L-J 】 -

Filter
Choose ： Item - QC lot number –reagent
card lot number - level ， draw
Default item setting
Default item setting
Precautions for the use of the instrument
First see ： Kit packaging integrity (complete, damaged, scattered
reagent cards, buffer volume)

Second inspection: Check whether the deionized water and washing

liquid are sufficient, whether the connection between the filter and
the pipeline is tight, and check whether there is any abnormality such
as liquid leakage and hanging liquid during the power-on reset and
reset process.

Three confirmation: Confirm whether the consumables are loaded

correctly (position, direction, sealing strips, and stop bars are in
place); Confirm whether the ID chip information of the kit matches
the reagent card buffer and other information; Confirm the operating
mode of the instrument (customer mode, administrator mode) )

Four Note: Pay attention to whether the blood sample volume of the
blood collection tube is sufficient
Component
s
 Control——Slave control board
Cleaning solution
Slave control board and buffer
(2.5 mL)

deionized water
and sample
(250μL)

Uploading area The micro switch

Salve Control Board
Control Board

Main Control Board

Core Board

Expansion Board
Main Control Board
Expansion Board
 Core Board
NB module
Touch screen Power （ 1
2V ）
cable
Fluorescent
USB line

Signal line
Serial port

Main board
battery （ CR
Network 1220 ）
port
CPU
 Touch Screen Connection

Touch screen
cable Signal line

Fluorescent
line
 Autosampler Unit
The micro switch

Function:
1. Detect whether the longitudinal sample
injection tube rack is pushed to the position,
and give a signal to the longitudinal sample
injection to reset it
2. Give the lateral injection a signal to work
 Autosampler Unit

Lateral injection unit

Lateral pushing block

 Autosampler Unit

Unloading sensor Unloading motor

Function: Induct whether the

push out test tube has
occupied the unloading area.
If the number is large, it will
alarm that the unloading
area is full. Please clear it.
 Autosampler Unit

Shaking Z
limit position
optocoupler
Shaking Y limit
position optocoupler
Shaking Z initial
position
optocoupler Shaking Y initial
position optocoupler
Positive side Reverse side Lateral side
 Autosampler Unit
Test tube clamping optocoupler
optocoupler checking
whether sample tubes exist
Function:
1. optocoupler checking whether
sample tubes exist ： Determine
whether there is a test tube. If
there is a test tube, give the claw a
ready instruction.
2. Test tube clamping optocoupler:
Determine whether the tube is
pinched.
 Consumables Unit
Reagent stock Buffer stock
Reagent stock Y motor
Reagent stock Y motor
initial optocoupler
 Consumables Unit
Card feeding component module

trumpet
Card feeding
initial
optocoupler
 Sampling Unit
Z Sampling motor
initial optocoupler

Sampling belt

Liquid inlet pipe

waste liquid pipe

Y Sampling motor
Swab
 Liquid Unit
V10 Dismantling figure
Mixing cup
Function:
container for mixing buffer
and sample

Isolation pool
Function: 1. Blow bubbles.
2. Isolate samples to prevent
contamination.

pinch valve （ v10 、 v11 ）

Function: A switch
that controls water
 Liquid Unit

Injection
Waste liquid pump Function: inhale and exhale Negative pressure tank
Function: To provide power system fluid with sample and Function: Collect and
for discharging waste liquid buffer by up and down motion store waste liquid
 Reaction Platform Unit
Discarding card
component

Reaction platform Discarding Discarding

shrapnel （ M-mode ） card belt card slider
 Reaction Platform Unit

AD acquisition
board
Restoration
optocoupler
Counter
optocoupler

Light source
acquisition initial
position optocoupler

Blue light collects T/C values and red light reads bar codes
 Temperature Control Unit

Temperature control board Temperature control board:

Including four temperature control modules
connected to the main control board J9

Reaction platform temperature sensor

Reagent chamber temperature sensor
Buffer chamber temperature sensor
Room temperature sensor
Common
Tools
 Common Tools

Hardware:
phillips screwdriver, hexagon screwdriver(No. 3), tweezers,
cutting pliers, flat pliers
Software:
single instruction, compound instruction, parameter setting,
other functional tests
Accessories:
optocoupler (4 kinds), screw (3 kinds), swab, filter, two-way valve,
cable tie, adhesive buckle
 Fault
Modularizatio
n Analysis
Autosampler
Unit
 Autosampler Unit
microswitch

Phenomenon: Is there any abnormal noise after

the longitudinal injection reaches the limit
position? Or prompt “Sample rack is finished. Add
more samples?”.
Analysis: The reason for the abnormal noise is that the
longitudinal injection pusher pushed all the time, and the
microswitch cannot sense it, so it cannot give a correct
signal. Check whether the microswitch circuit falls off.
Temporary solution: close the microswitch function
([Parameter] → [Autosampler unit] → [Function
Configuration] → [Test tube rack switch])
 Autosampler Unit

Longitudinal injector component

Phenomenon: After pushing to the
transverse injection area, the test tube rack
cannot conduct transverse injection.
Analysis: The pusher spring is loose, which makes it
unable to return to the correct position. It is
recommended to replace the longitudinal injection
component.
 Autosampler Unit
Lateral injection component
Phenomenon: the first sample doesn’t advance.
Analysis: During lateral injection, the pusher is blocked by
the platform baffle, and the spring cannot spring out. Or
prompt “Sample rack is finished. Add more samples?”.
Analysis: Swing the spring by hand to feel the elasticity. Is
the spring falling off?
If the spring force is not enough, loosen the screws slightly.
If the elasticity is normal, loosen the screws that fix the
lateral injection assembly, push it outward to the limit
position, and then tighten the screws to ensure that the
lateral push block is in the middle of the platform plate.
 Autosampler Unit
Problem: Optocoupler checking whether sample optocoupler checking
tubes exist or test tube clamping optocoupler whether sample tubes exist
misjudge.
Analysis 1: Single command horizontal injection, observe whether
the optocoupler can fully illuminate the middle position of the tube
hole? Check whether the edge of the test tube rack blocks the
optocoupler, add shims to the optocoupler so that the optocoupler
completely shoots into the middle of the test tube rack, and check
whether the indicator light is normally on. (Avoid adverse
phenomena caused by light reflection)
Analysis 2: The deviation is large, and the ideal position cannot be
reached all the time. Adjust the working steps of transverse
injection to solve the problem
Analysis 3: There should be no bending at the optocoupler line. It is
also possible that the reflection drive board is poor or inversely
connected. You can try to replace the reflection drive board. test tube clamping optocoupler
Analysis 4: Light reflection.
Autosampler Unit

Indicator light of optocoupler checking

whether sample tubes exist

Indicator light of microswitch

Indicator light of test tube clamping

optocoupler
Autosampler Unit
Problem: The lateral injection of test tube rack
is not smooth.
Phenomenon: When the lateral injection goes
to the left, there will be abnormal noise or it
can not go out smoothly.
Analysis 1: Check whether the baffles on both sides are too tight,
or there is interference at the bottom of the rack, and the friction
is large.
(Only adjust the lower cushion block to ensure that the baffle and
cushion block are on the same horizontal line)
Analysis 2: The elasticity of the lateral push block is not enough
(Adjust the position with the strongest spring elasticity)
Analysis 3: The screw fixing the spring is loose, and the push
block does not rebound.
 Autosampler Unit
Uploading sensor
Problem: no result when the countdown is
0.
Temporary treatment method:
Reverse the sensor drive board and remove the
optocoupler terminal wiring to ensure that the
optocoupler indicator light is always on
Principle: If there is a test tube rack in the
unloading area, it will affect the photoelectric
reading value and kicking card.
During training, teachers should also be reminded
Problem: The unloading area is full, and
not to put anything in the unloading area.
there is no alarm.
Analysis 1: The connection of the unloading
sensor is uncoated, resulting in poor contact.
Analysis 2: The unloading sensor is disconnected.
 Autosampler Unit
Problem: Front and rear motor initial position optocoupler fault.
Symptom 1: Shaking claw cannot reset normally.
Analysis: The initial position optocoupler of the shaking Y motor is broken.

Phenomenon 2: Shaking claw keep walking and do not stop with abnormal
sound.
Analysis 1: The limit position optocoupler of the shaking Y motor is
broken.
Analysis 2: The optocoupler baffle is too short and the optical coupler
cannot sense.

Phenomenon 3: Shaking claw only goes half way.

Analysis 1: Abnormal parameter of the maximum working step of shaking
Y motor (5000).
Analysis 2: The optocoupler baffle is crooked and cannot enter the
optocoupler normally.
 Autosampler Unit

Phenomenon: Shaking claw can not reset.

Analysis 1: The initial position of the shaking motor

optocoupler is faulty.
Analysis 2: The wiring of shaking Y motor is abnormal.
Analysis 3: The parameter of shaking Y motor
compensation step is abnormal.
Consumable
Unit
 Consumable Unit
Reagent Buffer Phenomenon: Buffer cannot be added
stock stock There is deviation in the position of the buffer
stock, or buffer is placed in the wrong direction, or
buffer cover is not opened.

Analysis I: Inadequate training results in errors.

Analysis II: If there is a deviation in the position, adjust the
compensation steps of the buffer stock.
Analysis III: There is no volatilization of new packaging buffer.
The old package needs to remind the teacher not to put it in
the instrument for too long.
Analysis IV: Water leakage, blocking the optocoupler,
misidentification.
 Consumable Unit
card feeding Problem: Abnormal card feeding
component Analysis I: Check whether the card pushing block is in the middle of the
reagent card slot. If not, adjust the Y compensation steps of
reagent stock (position 4 is the initial position) and only
change the Y compensation steps of reagent stock and the
working steps of each bin (the third group of the main
control unit, only change the 123 bin)
Card feeding Analysis II: Check whether the card pushing block is too long. Polish the
initial front end of the card pushing block to push it to the middle
optocoupler of the reagent card.
Analysis III: If the card pushing block cannot be reset and there is
abnormal noise, check the initial position optocoupler of
card feeding. Check whether the optocoupler blank is in the
middle of the optocoupler. If it cannot reach the middle of
the optocoupler, extend the length of the optocoupler.
Analysis IV: The screw fixing the card feeding component on the belt is
trumpet loose, which causes the card feeding component to be
pushed empty and does not drive the belt to move.
 Consumable Unit

Analysis V: The reagent cards in the old reagent card box are
disordered, which causes that the card cannot be pushed or cannot
be pushed.
Analysis VI: Push the empty card, the software setting is abnormal
with the actual placement, or the spring is not elastic enough to
spring up.
Analysis VII: The black rubber plug is forgot to remove , which is
stuck between the bell mouth and the inlet
Analysis VIII: Abnormal installation of card feeding components
Analysis IX: The card feeding push block is too long, so it is blocked
by screws and cannot be reset.
Analysis X: The limit screw does not have a sleeve, resulting in
excessive height. Or too low after casing.
 Consumable Unit
Initial optocoupler of reagent
reagent stock Y motor stock Y
motor

Problem ： Reagent card bin cannot be reset

Phenomenon: Abnormal noise during resetting
Analysis I: Check whether the Y direction optocoupler of reagent
bin is normal.
Analysis II: Check whether the motor is normal and whether the motor
drive is normal.
Analysis III: Check whether the hardware structure is obstructed.
 Sampling
Needle Unit
 Sampling Needle Unit
Phenomenon: Sampling needle can't descend to the correct
position.
Analysis I: Sampling needle is pricked on the tube cover and could not be pricked down. If
it is forward and backward, adjust the distance parameter between the initial position
and the sampling position; if it is left and right, adjust the number of horizontal injection
steps.
Analysis II: Sampling needle is pricked to other positions at the mixing cup or buffer
position. The mixing cup position requires that the needle be at the center of the cone at
2-3mm above the bottom of the cup; The buffer position is at the center of the bottle
mouth (adjust the parameters of the buffer component when necessary); The sampling
position is at the center of the tube cover.
Analysis III: The sampling needle is not in the middle of the reagent card hole, resulting in
abnormal sample adding or liquid shedding. If the front and back offset, need to adjust
the sampling Y motor compensation step. If the left and right deviation, need to adjust
the card working step number.
Analysis IV: The bearing of sampling Z component is loose, causing blood lancet to shift.
Analysis V: The sampling Z belt (front and rear) is seriously worn or broken.
 Sampling Needle Unit
Phenomenon: Sampling needle Z motor cannot be reset.
Analysis I: The initial optocoupler of sampling needle is damaged,
replace the optocoupler.
Analysis II: The front and rear belts of the sampling components
are seriously worn, the sliding rod is bent. Replace the component.

Phenomenon: No sample is sucked into the pipeline above

sampling needle.
Analysis I: The pipeline is bent, cannot be hidden under the baffle.
Analysis II: At V7, three-way connector is loose.
Analysis III: Blood lancet is blocked.
Liquid Unit
 Liquid Unit
Phenomenon: Overflow of mixing cup
Analysis I: V11 is blocked, replace v11, if not, clean V11.
Analysis II: The pipeline between mixing cup and isolation tank is
disconnected. Mixing
Phenomenon: During the mixing process, the mixing
cup
cup does not float up with bubbles
Analysis I: Whether the isolation tank is damaged.
Analysis II: Syringe A is abnormal, judge by resetting.
Analysis III: V01 is broken or the pipeline of V01 is disconnected.
Phenomenon: The liquid in the mixing cup cannot be
drained
Isolation
Analysis: The filter screen of the filter is damaged or causes the pool
V10 to be blocked, there is debris at the bottom of the mixing
cup to block the mouth, the negative pressure pump is lost, the
pipeline is airtight, and whether the negative pressure tank is
cracked.
 Liquid Unit
Phenomenon: Leakage
Analysis I: V10 is blocked or broken, causing the waste liquid pipe at the
swab not to drain
Analysis II: The position of needle and swab deviates, compensating steps
of adjusting needle (140-220) (water overflowing at the top of swab)
Analysis III: The diameter of the swab is worn and becomes larger, causing
V10 Dismantling figure
water leakage. Replace the swab
Analysis IV: The negative pressure tank is damaged, and the pipeline (Y)
connecting the negative pressure tank with swab and V10 and V11 is
airtight. Replace the negative pressure tank
Analysis V: Y way of V7 and V3 is loose.
Analysis VI: Water drop type leakage, check whether the pipe above the
blood lancet is bent and not fixed at the correct position (not under the
baffle).
Analysis VII: Whether the swab pipeline is loose, and the pipeline should
not be too long.
Analysis VIII: The filter is blocked and a lot of residues are accumulated.
 Liquid Unit
Phenomenon:There are always a few
bubbles in the liquid path.
Analysis I: There is always a small amount of bubbles in the
liquid circuit. Whether the pipe joints of the syringe are loose
enough to cause bubbles must be removed with tweezers
Analysis II: Whether the liquid circuit at the connection
position of the liquid inlet pipe (external connector and
internal connector) falls off, causing bubbles to enter.
Analysis III: Whether the pipeline in the barrel floats.
Analysis IV: Whether the pipe filter in the barrel is blocked,
causing bubbles.
Analysis V: Whether the syringe has extrusion pipeline.
Analysis VI: Whether the valve pipeline is pressed by the
front housing.
Analysis VII: Does V3/V7 valve fall off?
Negative pressure tank
 Liquid Unit

Summary of syringe problems

1. Improper steps of the syringe will lead to abnormal sample
adding
2. There will be abnormal noise when walking too much. Adjust
the compensation steps
3. sliding rod crooked, unable to reset
4. Optocoupler fault, unable to reset
5. Damaged or loose connector
 Liquid Unit

V1 Mixing cup
Swab waste liquid V11
0 waste liquid
V Negative
filter Isolation pool 1
pressure
swab mixing cup tank

Clean the inner wall of

the blood lancet Waste liquid
SMA pump
V V
V 7 3 V
9 5
V V Power source
8 4 of air bubbles
Cleaning liquid Deionized water
Reaction
Platform
Unit
 Reaction Platform Unit
Phenomenon: Abnormal Discarding Card
Analysis I: The belt of the discarding card component is too loose or tight, the
sliding rod is crooked and lacks oil, the wear degree of the discarding card slider, and
the angle of the paddle Discarding card
Analysis II: The relative position with the reaction disk is abnormal. (It is necessary
to adjust the steps for compensating the discarding card position of the reaction
component
disk)
Analysis III: Slide angle problem (add a magnet block or fastener, expand the
opening)
Analysis IV: Abnormal initial position optocoupler (limit
optocoupler can be used as standby)
Analysis V: The reaction disk shrapnel is too tight, use flat
pliers to loosen it, and be careful not to break it.
Analysis VI: The running speed of card eliminatin motor is not
enough, increase the running speed (solved temporarily).
Analysis VII: The kicker slider is seriously worn, causing the
discarding card paddle angle offset.
 Reaction Platform Unit

How to replace reaction disk shrapnel?

1. Take out with tweezers
2. Invert M type to W type
(The hold at two points is changed to that at one
point)
3. Insert one end of the spring piece, then bend it
slightly and insert the other end.
 Reaction Platform Unit
Phenomenon: The light source acquisition cannot be
reset, resulting in no action after the countdown is 0.
Analysis I: Check whether the system time is still. If it is still, the core board is
damaged. If the time is normal, check whether the discarding card is successful.
If there is no problem with the discarding card, check whether the optocoupler
at the light source is normal.
Analysis II: The AD acquisition board fails, and the AD acquisition wiring can be
replugged.
Analysis III: It is believed that there is a test tube rack at the unloading position
of the inductive optocoupler, which causes this phenomenon. The reflection
sensor can be reversely connected and the wiring near the optocoupler can be
removed.
Analysis IV: The reaction disk reset optocoupler is abnormal, the optocoupler
wiring is bent, the optocoupler blank is not in the middle of the optocoupler, Optical components
the blank length is not long enough, or the optocoupler cannot sense the
optocoupler blank.
Analysis V: The light source initial position sensing optocoupler is abnormal,
and the wiring is interfered.
 Reaction Platform Unit

Phenomenon: Abnormal light source

Analysis I: If the alarm fails to read the barcode, the recheck command can be performed to test the
red light source, observe whether the light source emits red light, and check whether the read barcode
is consistent with the reagent card barcode. The temporary solution is to turn off the red light. (Full red
light: detect barcode and card; half red light: only detect card)
Analysis II: If it is suspected that the test result is abnormal, the reagent card just tested can be tested
with the recheck command for photoelectric collection and timely recheck.
Note: After the light source is replaced, the photopotential needs to be adjusted with a black card.
 Reaction Platform Unit

Temperature Phenomenon: Temperature out of

control board control, power on reset failed.
Analysis: Check the version number, the code of the
temperature control board are garbled.
Common
Problem
 Common Problem
Problem: How to judge motor fault
Analysis I: Single command operation corresponds to motor reset or fixed steps
Analysis II: Check whether the corresponding optocoupler of the motor is normal
Analysis III: Check structural parts for interference or damage
Analysis IV: Try to replace the drive board to see if the motor can work

Problem: How to judge optocoupler fault

When the machine is powered on, a blank is used to cover the optocoupler. The
optocoupler indicator light can be normally off. When the blank is removed, the indicator
light will be on. The indicator light will flash when shifting gear and not shifting gear
 Common Problem
Software Version ：
Problem ： Communication V2.0.0.93.0.50.0.27.0.43 （ Main control
error board 、 Core board, slave control board
and temperature control board ）
Phenomenon: power on and resetting all
the time
Analysis I: Check whether the version number is
complete, and if not, judge where the
communication is not interrupted.
Analysis II: Check whether the motor or
optocoupler is abnormal
Analysis III: Check the circuit for poor contact (J7
connecting core board, J8 connecting slave
board, J9 connecting temperature control board,
J6 connecting expansion board)
 Common Problem
Screen related issues
1. The screen is not sensitive: Calibrate screen
2. Abnormal touch screen: The touch screen cable
is disconnected or the screen driver board is
abnormal. It is recommended to replace the
screen.
3. Blurred screen: The screen signal wire (black) is
damaged or in poor contact.
4. Flash screen/white screen ： Poor contact of
fluorescent wire.
5. If the system time is restored to the factory
setting, the core board battery is out of power
(CR1220).
 Common Problem
Problem ： Reagent card does not add sample or the sample amount is
insufficient, indicating abnormal sample adding
Analysis of reagent problems:
1. Check the amount of reagent, buffer solution and the sample migration on the reagent of waste
cards;
2. Looking at the sample curve, especially the value of Line C, if the value is less than the preset value
of the ID card (mostly 3000), the basic judgment is that the liquid absorption is insufficient.
Note: No sample migration on the reagent of membrane may be an accidental quality problem of a
reagent card. Only when batch sampling is insufficient can it be the fault of the instrument.
Analysis of analyzer problems ：
1. The instrument syringe leaks or presses the pipeline 2. Abnormal of V07 valve and V03 valve
3. Abnormal blood lancet pipeline 4. Abnormal external fluid circuit connector pipeline
5. Insufficient buffer liquid 6. Inadequate perfusion
7. Abnormal sampling position of blood lancet 8. Loose internal pipeline
 Common Problem
Problem: The repeatability of the instrument is poor
The perspective of reagents:
1. Confirm the batch, preferably the same lot of reagents.
2. Confirm the reagent unsealing time and whether the buffer volume are sufficient.
3. Confirm sample type and sample size.
The perspective of instruments ：
1. How long is the mixing cup used, whether there is residue and whether it is too
dirty.
2. Check the tightness of the fluid circuit (7 directions).
3. Check whether the bubbling function is normal, and observe whether each sample
has bubbling.
4. It may also be caused by the instability of the light source of the laser module (this is
very rare).
 Common Problem
Problem: Instrument crash
Fault I: The screen doesn't respond, but the system time is running.
Analysis: The touch screen cable or the screen driver board is broken. It is recommended to
replace the screen. The temporary solution is to connect a mouse to operate the sample. (The
mouse cursor is not obvious and needs to be observed carefully)
Fault II: The software doesn't respond, and the system time stops.
Analysis: The core board is broken, replace it.
Fault III: There is no result in the countdown, but the system time is running.
Analysis: The discarding card component or optocoupler at light source is abnormal, and the
test needs to be restarted after the point is stopped.
Fault IV: The software does not respond, and the time display is normal. Apply for the specimen
after restart, and the system will crash again after a while.
Analysis: Understand the temperature of the department where the instrument is located. If
the temperature is too high, it is necessary to abstract heat the core board CPU.
 Common Problem

Problem ： Abnormity in automatic sample injection pretreatment

Phenomenon ： Only run the reagent rack, not the sample.
Analysis I: Whether the test tube has abnormal optocoupler.
Analysis II: Software bug, repairing.
 Common Problem
Reason for upgrade failure and how to downgrade?
Reason for upgrade failure:
1. U disk mismatch, medium virus
2. There are multiple upgrade packages in the root directory of the USB flash drive
3. The upgrade package is not unzipped, resulting in failure to upgrade
Degradation method:
1. Photographic parameters, lis
2. Back up version 98 data to the computer desktop.
3. The data in other files of the installation package is moved to the root directory.
4. Upgrade.
5. Manual configuration lis.
 Common Problem
How to Repair Damaged DATA Data in Software ？
1. Open installation file 2. Open other related materials
4. Copy the data package
Back to the installation file home
page
3.Continue to open the folder Only the DATA package is
containing all DATA displayed in the wfuptrade folder
All other files are deleted
Insert the USB flash disk for
upgrading to repair the DATA
Only retain other relevant data data
Delete redundant data
 Common Problem
Problem: Analysis of sample results and clinical inconformity
The card pushing block is too high
Hemolysis occurs, 1. valve 10 or valve 11 is blocked. 2. valve is
burnt 3.The mixing cup is blocked by test tube cap debris

The reagent
card has
hemolysis

Analysis:
1. Valve 10 or valve 11 is blocked, or the valve is burnt
2. The bottom of the mixing cup is blocked by the debris
Hemolysis of the test tube cap, causing the water cannot be drained
Waveform
recognition
 Principle
During detection, the object to be detected in the
sample forms an immune complex with the
fluorescently labeled antibody, and undergoes a
chromatographic process and solidifies in the
detection area and quality control area,
respectively. When the reagent card is transported Photoelectric converter
to the detection area, the LED blue light source of
the analyzer shines to the detection area of the Light source
reagent card and the fluorescent immune complex
Fluorescent signal
in the quality control area to excite the
fluorescence to emit light, the light waves emitted
by the fluorescent matter are collected by the
signal acquisition board and converted into
electrical signals, the strength of the electrical
signal depends on the concentration and number
of fluorescent molecules, analyzer calculates the
concentration of the analyte in the sample to be
measured according to the strength of the
feedback signal.
 Value

The final concentration value is determined according to the functional relationship between the
T/C value and the concentration
 Waveform recognition

1. CRP waveform

CRP waveform
consists of two
waves, C wave and T
wave, T wave in the
front and C wave in
the back. The peak T
range of T wave is
200~400, and the C
peak range of C wave
is 400~600.
 Waveform recognition

2. PCT waveform

PCT waveform consists

of two waves, C wave
and T wave, C wave in
the front and T wave in T
the back. The peak
range of C wave is C
0~200, and the peak
range of T wave is
200~400.
 Waveform recognition

3 、 Waveform of
cTnl/CK-MB/MYO
Waveform of cTnl/CK-
MB/MYO is four-segment
waves, one C wave and
three T waves, the C
wave in the front and the
T wave in the back. The T-ck
peak range of C wave is
0~150, the peak range T-CTnI
of T-CK wave is C T-MYO
150~300, the peak
range of T-CTnl wave is
300~450, and the peak
range of T-MYO wave is
450~600.
 Waveform recognition

4 、 NT-proBNP
waveform
NT-proBNP
waveform consists
of two waves, C
wave and T wave, C
wave in the front
and T wave in the
back. The peak T
range of C wave is C
200~400, and the
peak range of T
wave is 400~600.
 Waveform recognition
5 、 cTnl
waveform
cTnl waveform
consists of two
waves, C wave and T
wave, C wave in the
front and T wave in T
the back. The peak
range of C wave is
C
200~400, and the
peak range of T wave
is 400~600.
 Waveform recognition

6 、 DD
waveform
DD waveform
consists of two
waves, C wave and
T wave, C wave in T
the front and T wave C
in the back. The
peak range of C
wave is 200~400,
and the peak range
of T wave is
400~600.
 Waveform position offset
The T and C lines of the waveform have corresponding value intervals (segment points). Within the corresponding inte
CRP as an example, T2 peak interval: 201-400, C peak interval: 401-580), take out the abscissa of maximum fluorescen
the peak position of the T line and the C line. If the waveform position shift range is too large, the T peak and C peak a
out of the value range, which will lead to wrong T peak and C peak value in the original value range, and the result of T
wrong. The concentration of the fluorescence value inversely calculated from the wrong T2/C and calibration curve will

The range and width of the T line and C line of FS-301 items are different (see the appendix for the value range). There
avoid abnormal results due to the offset of the waveforms of other items, the peak position needs to be controlled with
range. Taking the CRP project as an example, the two peak positions should be controlled at 300±20(T2) and 500±20(C

300 500 300 500 300 500

Normal waveform Left waveform Right waveform
 Waveform Position Offset – Adjustment Process Of Waveform Position

If the waveform position shift causes the wrong peak

value of T peak and C peak, the test result will be
abnormal, and the waveform position needs to be
adjusted. The adjustment process is shown in the chart
on the right.
To adjust the waveform position, it is recommended to
use a standard fluorescent color card to collect blue light
through compound commands, check the waveform in
the [History] interface and adjust the waveform position.
If there is no standard fluorescent color card, you can
replace it with reagents of other items, perform periodic
test, and observe the waveform position.
The two peak positions of the CRP project should be
controlled in the interval: 300±20, 500±20; the two
peak positions of the PCT project should be controlled in
the interval: 100±20, 300±20
 Automatic Waveform Position Offset – Sampling needle and sample hole position offset
If the tip of the sampling needle is offset from the position of the sample hole of the reagent card,
you need to
enter the interface of "Function~Maintain~Parameter~Consumables Unit~Card feeding motor"
interface, and adjust the parameter of “Working steps of card feed motor". According to the
amount of offset, correspondingly modify the parameter "Number of steps for card-feed
motor“(Adjust according to 1mm=80 steps).
Position Left sampling needle Right sampling needle

Schematic
diagram

Method Decrease the parameter of Increase the parameter of

"steps of card feeding motor" "steps of card feeding motor"
 Waveform position adjustment method
Adjust sampling Y
Sample well deviation motor compensation
steps
The sampling
采样针加样时
needle did not
样本没有吐在
add in the
试剂卡的加样 Increase of
sample well of
孔中 采样 Y 电机补Y
Sampling
the reagent card motor
偿步数增大
compensation
steps

The sampling needle Deviated sample well ,

with sample has not adjust sampling Y motor
add into sample well of compensation steps
reagent card
 Fully automatic waveform position offset – Appendix

Sign T/C type Segment point Peak position

No. Item name

1 CRP T1,T2,C T2/C 0-200,201-400,401-580 300,500

2 PCT C,T2,T1 T2/C 0-200,201-400,401-580 100,300

3 NT-proBNP T1,C,T2 T2/C 0-200,201-400,401-580 300,500

4 D-Dimer T1,C,T2 T2/C 66-200,201-400,401-534 300,500

Photoelectric
potential
calibration
method
Photoelectric potential calibration method

1. Photoelectric potential calibration

card test
Take 1 photoelectric potential calibration card
(black),by 【 compound instruction test 】 interface ，
select the No. 1 disk ， the “Insert Card” and
“Photoelectricity Acquisition” items, click [Test]
button, the waveform of photoelectric potential
calibration will be displayed on the right side of the
interface. Notice: 1. Record the approximate
average value of horizontal segment of the
waveform as basis of “result cutoff value“of next
setting of photoelectric potential calibration. 2. Place
the slit of photoelectric potential calibration card
facing up, away from the handle end of the reagent
card.
Photoelectric potential calibration method
2. Modification of common compensation
parameters
In the [Parameter Configuration] interface,
select of "Compensation Steps (Optical 2) “of [
Reaction platform unit] ， will be “Share the
same compensation (0: common; 1: not
common)" with parameter setting is
1 。 Notice ： 1. Start the photoelectric potential
calibration of blue light in each disk ， need set
up “Share the same compensation (0:
common; 1: not common)“with parameter
setting is 1 ； 2. Parameter is 1, the
photoelectric position compensation of 20
channels will use parameters of
"Compensation Steps” individually (Optical 1/
Photoelectric potential calibration method
3. Photoelectric potential calibration
parameter setting
On the [Compound] interface, select
[Photoelectricity Calibration] , set the
“Steps of Initial Offset“ and "Result Of
Valve Value", other parameters are as
above without modification 。 Notice ：
recorded photoelectric position
compensation steps" minus 10~15 as
“Steps of Initial Offset“ ； recorded
average value from stable segment of
the waveform of the potential
calibration card minus 30~50 as "Result
Of Valve Value“.
Photoelectric potential calibration method

4. Photoelectric potential calibration test

Set the disk to be tested (the starting and ending disk
parameters are the same), put the photoelectric potential
calibration card into the reagent card, click the [Test]
button, instrument will automatically push in the card and
perform calibration. Notice ： During the photoelectric
potential calibration, the turntable is rotate many times
based on the “Steps of Initial Offset" （ compensation steps
for the next rotation is increased by 1/4 step than the
previous one ， increased steps depends on the current
fluorescence value and the ½ result cutoff value ）， every
time the reaction platform rotates, the blue light will scan
the photoelectric potential 。
Photoelectric potential calibration method
5. Auto calibration complete interface
If it cannot be detected the best position
within the “maximum number of detections” ，
will display [Calibration Failed] ； If it can be
detected the best position within the
“maximum number of detections” ， will display
[Auto Calibration Completed] , the interface
displays the compensation steps of current
disk 。 Notice ： 1. :It will automatically kick out
the photoelectric potential calibration card in
the platform after the calibration is completed ；
2. If the calibration fails, you can re-change the
parameters of “Steps of Initial Offset”and
“Result Of Valve Value”,then recalibrate 。
Photoelectric potential calibration method

6. Save the compensation steps

parameter
After completing the photoelectric
potential calibration of a disk, enter the
[Parameter] ， select "Compensation
Steps (Optical 1/Optical 2)” of [Reaction
platform unit] ， click [Save] button to
save the compensation steps of
automatic calibration. Repeating the
photoelectric calibration for the
remaining disk(2-20) until fully
preserved 20 disks of photoelectric
compensation steps 。
Fully Automated Blue light Debugging Method – Appendix: Debugging Tools

Common reagent card

Photoelectric potential calibration card

 Automatic Blue light debugging method - appendix:
description of parameter setting of photoelectric potential
calibration
Compensation for the
The disk to be calibrated, first turn of the
the parameter between platform.
starting disk and end
disk are same.

Platform movement Maximum detection times

parameters (Default) ） of photoelectric potential.
The current detected
The reference
fluorescence value is
standard for the
less than 1/2 of the
current detection
result quality value.
fluorescence value.
Increase the number
of compensation
steps each time.
The current detected
fluorescence value is Start/end position of blue
more than 1/2 of the light detection(Default)
result quality value.
Increase the number
of compensation
steps each time
THANK YOU!
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