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Core Practical 2 PPT

The document outlines a method to investigate the vitamin C content in various drinks using DCPIP as an indicator. It details the theory behind the reaction, the step-by-step method for conducting the experiment, and includes safety precautions. Additionally, it presents math skills and questions related to the investigation to enhance understanding of the results.

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khushaallk1
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2 views

Core Practical 2 PPT

The document outlines a method to investigate the vitamin C content in various drinks using DCPIP as an indicator. It details the theory behind the reaction, the step-by-step method for conducting the experiment, and includes safety precautions. Additionally, it presents math skills and questions related to the investigation to enhance understanding of the results.

Uploaded by

khushaallk1
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd
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INVESTIGATE THE

VITAMIN C CONTENT
OF FOOD AND DRINKS
Khushaal LK
Table of contents
01 Theory

02 Method

03 Calculations
Introduction
To compare vitamin C levels in different
drinks by reacting them with DCPIP, by
estimating volume required to reduce
DCPIP observed by colour change from
blue to colourless. Allowing to calculate
concentration of vitamin C present in
different drinks.
01
Theory
Theory
Vitamin C is a strong reducing agent. Therefore, when added to
DCPIP(dichlorophenolindophenol) it is reduced causing colour change from blue to
colourless.

It involved gradually adding known concentration of Vitamin C solution(standard solution)


to DCPIP and obtain mean titre required to completely oxidise the DCPIP, indicated by
endpoint(blue to colourless).

Then a juice with unknown concentration of Vitamin C can be added to same volume of
DCPIP to obtain volume of juice required to reduce DCPIP. Then using the formula,
C1V1=C2V2
02
Method
Method
Safety Precautions

● Wear eye protection(DCPIP is an


irritant).
1 Use the 5 cm3 syringe to draw up 5 cm3 of 1% DCPIP. Shake the syringe to ● Avoid skin contact with the
expel any air bubbles. DCPIP and test tube solutions(As
2 Add 1 cm3 of DCPIP to a test tube. DCPIP is strong irritant and can
3 Use a clean 5 cm3 syringe to draw up 5 cm3 of the 1% vitamin C solution. stain clothes).
4 Add the vitamin C solution to the test tube containing the DCPIP, one ● Do not taste the fruit juice(As it
drop at a time. After each drop, shake the test tube slightly to ensure the may be expired).
solutions have mixed.
5 Continue to add vitamin C solution until the blue colour of the DCPIP
disappears.
6 Record the volume of vitamin C solution added. You can find this volume by subtracting the value on the syringe
from the original 5 cm3 in the syringe.
7 Repeat steps 2–6 twice more and calculate a mean value for the volume of 1% vitamin C solution needed to
decolourise 1 cm3 of DCPIP.
8 Add 1 cm3 of DCPIP to a clean test tube.
9 Use a clean syringe to draw up 5 cm3 of a fruit juice. Add the fruit juice to the DCPIP one drop at a time.
Record the volume of juice needed to cause the blue colour to disappear.
10
11 Repeat steps 8 and 9 twice more, then calculate the volume of fruit juice needed to decolourise 1 cm3 of DCPIP.
Repeat steps 8–10 with the other fruit juices.
03
Math
Skills and
Question
s
Math Skills

To be able to plot graphs to


estimate titre of different
concentration of Vitman C in juices
required to reduce DCPIP
Questions
(a)(i) State the independent and dependent variables in this investigation (a)(ii) State two variables, other than the volume
and concentration of DCPIP, that should be controlled in this investigation.​

(a)(ii) State the independent and dependent variables in this investigation.​

(a)(iii) The student found that 6 mg of vitamin C decolourised 1 cm³ of 0.1% DCPIP solution. Calculate the vitamin C content
of the ripe mango extract. Show your working.

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