Maglumi Service Software

--Overseas Technical Service Department

FOCUS MAKES PROFESSION

CONTENTS

0
PART ONE Moving Parts

1
0
PART TWO
Functional Parameters

2
0
PART THREE Macro

3
FOCUS MAKES PROFESSION
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snibe923 FOCUS MAKES PROFESSION 3/50

0
PART ONE
Moving Parts

1 FOCUS MAKES PROFESSION

0 Moving Parts
PART ONE

1
1.1 Incubator

FOCUS MAKES PROFESSION 5/50

0 Moving Parts
PART ONE

1
1.1 Incubator

a. <Writeparam> &
<Initialize>

b. Choose Stepsize

c. Initialize
Last
Incload
Backtr

d. Incubator
Position:
Incload is
selected,
adjust Pos 1-16
FOCUS MAKES PROFESSION 6/50
0 Moving Parts
PART ONE

1
1.1 Incubator

a. Place cuvettes in the

path

b. Check if 2 cuvettes align

perfectly with each other

c. If not, move incubator to

align;
if so, Click “Writeparam” to
save

FOCUS MAKES PROFESSION 7/50

0 Moving Parts
PART ONE

1.2 Wash Trans

1

FOCUS MAKES PROFESSION 8/50

0 Moving Parts
PART ONE

1
1.2 Wash Trans

FOCUS MAKES PROFESSION 9/50

0 Moving Parts
PART ONE

1
1.2 Wash Trans

a. The Max Step determines how far the cuvette will be

pushed.
b. The Min Step determines how far the loader will move
back.
c. Please make sure the loader moves back far enough to
push the cuvette again.
When cuvette cannot pass the wash transport , check if the
transport gear screws fixing well , if not, fix screws first; if
so, adjust Max/Min step.
FOCUS MAKES PROFESSION 10/50
0 Moving Parts
PART ONE

1
1.3 Wash Lift

FOCUS MAKES PROFESSION 11/50

0 Moving Parts
PART ONE

1
1.3 Wash Lift

a. <Writeparam>
& <Initialize>

b. Choose Stepsize 5

c. Initialize: waste needle

higher than cuvette
2mm
Inject: inject needle tip
align to the cuvette
surface
Suction: waste needle
touch cuvette bottom
and up1mm
Prime: waste needle FOCUS MAKES PROFESSION 12/50
0 Moving Parts
PART ONE

1
1.4 Chamber Trans

FOCUS MAKES PROFESSION 13/50

0 Moving Parts
PART ONE

1
1.4 Chamber Trans

a. Disconnect cables of
PMT and the reference
LED, take away the
cover of the chamber

b. <Writeparam> &
<Initialize>

c. Load Pos

b. Adjust Pos

FOCUS MAKES PROFESSION 14/50

0 Moving Parts
PART ONE

1
1.4 Chamber Trans

a. Input a value in Adjust

Pos.
b. Move the cuvette 4
steps (Recoup).

c. Check if the position clip

match the notch of the
cuvette.

d. If not repeat a-c. If so, it

should be fine.

e. Move the cuvette to the

inject position and check
if the head is at the
center of the cavity.

FOCUS MAKES PROFESSION 15/50

0 Moving Parts
PART ONE

1
1.5 Chamber Lift

FOCUS MAKES PROFESSION 16/50

0 Moving Parts
PART ONE

1
1.5 Chamber Lift

a. <Writeparam>
& <Initialize>

b. Choose
Stepsize

c. Initialize: cuvette can move

inside chamber
Transport: waste needle
higher than cuvette 2mm
Inject: inject needle higher
than cuvette surface 1mm
Prime: no gap between lift
and channel

FOCUS MAKES PROFESSION 17/50

0 Moving Parts
PART ONE

1
1.6 Pusher

FOCUS MAKES PROFESSION 18/50

0 Moving Parts
PART ONE

1
1.6 Pusher

a. <Writeparam> &
<Initialize>

b. Choose Stepsize

c. Init/Backtransport: Pusher to
Backtrans
Washer: Washer to Pusher
Chamber: Pusher to Chamber

Washer position should not align perfectly,

Pusher need extra 10 steps close to
backtransport
FOCUS MAKES PROFESSION 19/50
0 Moving Parts
PART ONE

1
1.7 Pumps

FOCUS MAKES PROFESSION 20/50

0 Moving Parts
PART ONE

1
1.7 Pumps
a. Condensate Int.

b. Soak time

c. Speed

d. Pump control

FOCUS MAKES PROFESSION 21/50

0 Moving Parts
PART ONE

1
1.8 Components that do not need adjustment

FOCUS MAKES PROFESSION 22/50

0 Moving Parts
PART ONE

1
1.9 Frequency and Regulation

a. Frequency: speed
of the motor

b. Regulation:
regulate the
reference point
for counting the
steps

FOCUS MAKES PROFESSION 23/50

0
PART TWO
Functional
Parameters

2 FOCUS MAKES PROFESSION

0 Functional
PART TWO

Parameters
2
2.1 Globals

FOCUS MAKES PROFESSION 25/50

0 Functional
PART TWO

Parameters
2
2.1 Globals

Functio Basic
n parameter
buttons s

Only when the corresponding component is

normal, and has been initialized, can these
buttons work normally.
FOCUS MAKES PROFESSION 26/50
0 Functional
PART TWO

Parameters
2
2.1.1 Prime W/C

FOCUS MAKES PROFESSION 27/50

0 Functional
PART TWO

Parameters
2
2.1.2 Background

a. Background/Closed
Background/Open

Background/Transport
pos:
b. Ref.
<80 LED on:
140,000-230,000
If not, change the
value in
D/A Ref Led

Background check should be performed in the first

place to ensure there is no light leakage in the
chamber and the reference LED is at proper state.

FOCUS MAKES PROFESSION 28/50

0 Functional
PART TWO

Parameters
2
2.1.2 Background

FOCUS MAKES PROFESSION 29/50

0 Functional
PART TWO

Parameters
2
2.1.3 Plateau

a. Max. RLU: 300000

Scale: 50000
<Draw Diagr.>

b. Reference LED on

c. Four times difference:

<5
Curve should be
d. smooth
Suggest D/A Highvolt

Plateau is to decide the high voltage for

PMT and to check the performance of PMT.

FOCUS MAKES PROFESSION 30/50

0 Functional
PART TWO

Parameters
2
2.1.3 Plateau

FOCUS MAKES PROFESSION 31/50

0 Functional
PART TWO

Parameters
2
2.1.4 Meas. Cuv.

a. Initialize in the main

interface

b. <Start Meas.>:
“please insert cuvette
in pusher ”
c. Insert a clean cuvette
in pusher, the third
notch match the
position clip
d. Result range: 200-
1200, CV<10%.
If not, check inject
needle, starter and
clean channel.
FOCUS MAKES PROFESSION 32/50
0 Functional
PART TWO

Parameters
2
2.1.5 System Test

FOCUS MAKES PROFESSION 33/50

0 Functional
PART TWO

Parameters
2
2.1.6 ErrorCard

Rang
e

FOCUS MAKES PROFESSION 34/50

0 Functional
PART TWO

Parameters
2
2.1.6 ErrorCard

FOCUS MAKES PROFESSION 35/50

0 Functional
PART TWO

Parameters
2
2.1.6 ErrorCard

+24 V: on the power

supply
+5V: on the 09-E00
board
+15V : on the 09-E00
board
-15 V : on the 09-E00
board
-5 V : on the 09-E00
board

FOCUS MAKES PROFESSION 36/50

0 Functional
PART TWO

Parameters
2
2.1.6 ErrorCard

FOCUS MAKES PROFESSION 37/50

0 Functional
PART TWO

Parameters
2
2.1.7 Min Value Cuv

FOCUS MAKES PROFESSION 38/50

0 Functional
PART TWO

Parameters
2 a. Go to <Background>. Measure the “Ref. LED
2.1.7 Min Value Cuv

on” value for 5 times and record the average

value X1.

b. Move the pusher to the “backtransport

position”, and put a cuvette in pusher. Move
the pusher to the “chamber position”.

c. Move the chamber transport 8 steps to make

the cuvette one cavity ahead the PMT in the
chamber.

d. Go to <Background> again. Measure the “Ref.

LED on” value for 5 times and record the
average value X2.

e. Min Value Cuv. = (X1 + X2) / 2 FOCUS MAKES PROFESSION 8/60

0 Functional
PART TWO

Parameters
2
2.1.8 RLU Factor

FOCUS MAKES PROFESSION 40/50

0 Functional
PART TWO

Parameters
2
2.1.8 RLU Factor

Sample/QC/Calibrator signal - BGW signal

RLU  RLU Factor
1000

Original RLU factor × Expected LC result

New RLU factor 
Average of measured LC results of both needles

FOCUS MAKES PROFESSION 41/50

0 Functional
PART TWO

Parameters
2
2.2 Wash

FOCUS MAKES PROFESSION 42/50

0 Functional
PART TWO

Parameters
2
2.2 Wash

Prime the needle

FOCUS MAKES PROFESSION 43/50

0 Functional
PART TWO

2.3 LLDParameters
2 (Liquid Level Detection)

FOCUS MAKES PROFESSION 44/50

0 Functional
PART TWO

2.3 LLDParameters
2 (Liquid Level Detection)

Choose the
position

Meaning of Delta
Z
and NoLiquid

FOCUS MAKES PROFESSION 45/50

0 Functional
PART TWO

2.3 LLDParameters
2 (Liquid Level Detection)
a. 4 tubes with
500uL distilled
Example: M2000, left pipettor
water inside
b. Put these 4 tubes
in sample area:
(T1, P1), (T1, P12),
(T12, P1), (T12,
P12)
c. Put 1 empty tube
in sample area:
(T6, P6)
d. 2 empty reagent kits
with 1ml distilled
Select
water added in Box 1
and Box 7
e. Insert the kits in
reagent area: T1 and
T15
FOCUS MAKES PROFESSION 46/50
0
PART THREE
Macro

3 FOCUS MAKES PROFESSION

0
PART THREE Macro

FOCUS MAKES PROFESSION 48/50

0
PART THREE Macro

FOCUS MAKES PROFESSION 49/50

 Thanks
FOCUS MAKES PROFESSION

FOCUS MAKES PROFESSION