Before the Opportunity Rover went dead- it collected organic material.docx
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The Opportunity Rover collected organic material from Mars, leading to an experiment isolating chromatin that resulted in DNA fragments of multiples of 410 base pairs. The consistent fragment size suggests an organized structure related to how DNA and proteins are associated in Martian cells. A longer digestion resulted in uniform 342 base pair DNA fragments, indicating a change in chromatin structure or organization.
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Before the Opportunity Rover went dead- it collected organic material.docx
- 1. Before the Opportunity Rover went dead, it collected organic material from Mars and returned it to Earth for analysis. You perform an experiment in which nuclear material (chromatin) is isolated from what appears to be Martian cells. You briefly digest with micrococcal nuclease which cuts accessible DNA. Any proteins are then removed and the resulting purified DNA is analyzed by gel electrophoresis. You observe a collection of DNA fragments (black bars) that are multiples of 410 base pairs in length (i.e., 410 bp , 820 bp , 1230 bp , 1640 bp . and so forth). What can you conclude about the organization of DNA and protein in the Martian sample? (Why 410 and why muliples fo 410 ) Suppose you perform an experiment in which the chromatin is digested for a mugh longer period of time with micrococcal nuclease prior to removal of chromatin proteins. When the resulting DNA preparation is analyzed by electrophoresis, all of the DNA appears as fragments 342 bp in length. What does this mean? (Why only 1 band and why 342 insted of 410 )