bioinfo_00-introduction.ppt

Introduction to Bioinformatics
Prof. Dr. Nizamettin AYDIN
naydin@yildiz.edu.tr
http://www3.yildiz.edu.tr/~naydin
1

2
Course Details
• Course Code : BLM 3810
• Course Name : Introduction to Bioinformatics
• Credit : 3
• Course Level : Undergradute
• Course web page:
http://www3.yildiz.edu.tr/~naydin/na_I2B.htm
• Instructors : Nizamettin AYDIN
Room: D-128
Email: naydin@yildiz.edu.tr, nizamettinaydin@gmail.com

3
Assesment
• Quiz : 10%
• Midterm : 25%
• Homework : 20%
• Final : 40%
• Attendance & participation : 05%
(The requirement for attendance is 70%)

Rules of the Conduct
• No eating /drinking in class
– except water
• Cell phones must be kept outside of class or
switched-off during class
– If your cell-phone rings during class or you use it
in any way, you will be asked to leave and counted
as unexcused absent.
• No web surfing and/or unrelated use of
computers,
– when computers are used in class or lab.
4

Rules of the Conduct
• You are responsible for checking the class web
page often for announcements.
– http://www.yildiz.edu.tr/~naydin/na_I2B.htm
• Academic dishonesty and cheating
– will not be tolerated
– will be dealt with according to university rules and
regulations
• http://www.yok.gov.tr/content/view/475/
• Presenting any work that does not belong to you is also
considered academic dishonesty.
5

Attendance Policy
• The requirement for attendance is 70%
– Hospital reports are not accepted to fulfill the
requirement for attendance.
– The students, who fail to fulfill the attendance
requirement, will be excluded from the final exams
and the grade of F0 will be given.
• Link for the rules and regulations:
http://www.ogi.yildiz.edu.tr/category.php?id=17
http://www.yok.gov.tr/content/view/544/230/lang,tr_TR/
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10
Recommended Texts - 4
Bioinformatics for
Dummies
Jean Claverie, Cedric
Notredame
Bioinformatics: A Practical
Guide to the Analysis of
Genes and Proteins
Andreas D. Baxevanis, B. F.
Ouellette, Ouellette B. F.
Francis.
Instant Notes in
Bioinformatics
D. R. Westhead, Richard M.
Twyman, J. H. Parish
Bioinformatics:
Sequence and Genome
Analysis, Vol. 5
David W. Mount, David
Mount
Developing
Bioinformatics Computer
Skills
Cynthia Gibas, Per
Jambeck, Lorrie LeJeune
(Editor)
Discovering Genomics,
Proteomics, and
Bioinformatics
A. Malcolm Campbell,
Laurie J. Heyer

11
Recommended Texts - 5
Structural
Bioinformatics
Philip E. Bourne (Editor),
Helge Weissig
Beginning Perl for
Bioinformatics
James Tisdall
Mastering Perl for
Bioinformatics
James D. Tisdall

12
Implants Artifical
organs
ECG
EEG
EMG
Ultrasound
Fuzzy Logic
Neural Nets
AI
instrumentatio
n
modelling
Simulation
sensors
MEMS
SoC
Wireles
MR
CT, X-RAY
Ultrasound
Empedan
s
biocompatible
agents
Chemical
composites
nanoparticles
Bioengineering

• The connectivity of the internet
(from the Wikipedia entry for
“internet”)
Introduction
13
• A map of human protein interactions
(from the Wikipedia entry for “Protein–
protein interaction”).
• We seek to understand biological principles on a genome-
wide scale using the tools of bioinformatics.

14
What is Bioinformatics?...
• A quick google search with the keyword
bioinformatics yields about
– About 37 100 000 results (0.65 sec.) (02. 03.2022)
• Synonyms:
– Computational Biology
– Computational Molecular Biology
– Biocomputing

• Bioinformatics
– the study of how information is represented and
transmitted in biological systems, starting at the
molecular level
is a discipline that does not need a computer!
– An ink pen and a supply of traditional laboratory
notebooks could be used to record results of
experiments.
– However, to do so would be like foregoing the use
of a computer and word-processing program in
favor of pen and paper to write a novel.
15
… What is Bioinformatics?...

• According to a National Institutes of Health
(NIH) definition, bioinformatics is
– “research, development, or application of
computational tools and approaches for expanding
the use of biological, medical, behavioral, or health
data, including those to acquire, store, organize,
analyze, or visualize such data.”
• The related discipline of computational biology is “the
development and application of data‐analytical and
theoretical methods, mathematical modeling, and
computational simulation techniques to the study of
biological, behavioral, and social systems.”
16

17
…What is Bioinformatics?...
• From Webopedia:
– The application of computer technology to the
management of biological information.
– Specifically, it is the science of developing
computer databases and algorithms to facilitate and
expedite biological research.
– Bioinformatics is being used largely in the field of
human genome research by the Human Genome
Project that has been determining the sequence of
the entire human genome (about 3 billion base
pairs) and is essential in using genomic information
to understand diseases.
– It is also used largely for the identification of new
molecular targets for drug discovery.

• Another definition from the National Human Genome
Research Institute (NHGRI) is that
– “Bioinformatics is the branch of biology that is concerned with the
acquisition, storage, display, and analysis of the information found
in nucleic acid and protein sequence data.”
• Russ Altman (1998) and Altman and Dugan (2003) offer two
definitions of bioinformatics.
– The first involves information flow following the central dogma of
molecular biology (next slide)
– The second definition involves information flow that is transferred
based on scientific methods. This definition includes problems
such as
• designing, validating, and sharing software;
• storing and sharing data;
• performing reproducible research workflows;
• interpreting experiments.
18
Altman, R.B. 1998. Bioinformatics in support of molecular medicine. Proceedings of AMIA Symposium 1998, 53–61. PMID: 9929182.
Altman, R.B., Dugan, J.M. 2003. Defining bioinformatics and structural bioinformatics. Methods of Biochemical Analysis 44, 3–14. PMID: 12647379.

19
https://www.youtube.com/watch?v=J3HVVi2k2No
https://www.youtube.com/watch?v=7Hk9jct2ozY
https://www.youtube.com/watch?v=9kOGOY7vthk
https://www.youtube.com/watch?v=gG7uCskUOrA
• A 1st perspective of the field
of bioinformatics is the cell.
– Bioinformatics has emerged as a
discipline as biology has become
transformed by the emergence of
molecular sequence data

• A 2nd perspective of
bioinformatics is the
organism.
– Broadening our view
from the level of the cell
to the organism, we can
consider the individual’s
genome (collection of
genes), including the
genes that are expressed
as RNA transcripts and
the protein products.
– For an individual organism, bioinformatics tools can therefore
be applied to describe changes through developmental time,
changes across body regions, and changes in a variety of
physiological or pathological states.
20

• A 3rd perspective of the field of
bioinformatics is represented by
the tree of life.
– The scope of bioinformatics
includes all of life on Earth,
including the three major
branches of bacteria, archaea, and
eukaryotes.
• Viruses, which exist on the
borderline of the definition of life,
are not depicted here.
– For all species, the collection and
analysis of molecular sequence
data allow us to describe the
complete collection of DNA that
comprises each organism (the
genome).
– We can further learn the
variations that occur between
species and among members of a
species, and we can deduce the
evolutionary history of life on
Earth
21

• From a practical sense, bioinformatics is a
science that involves
– collecting,
– manipulating,
– analyzing,
– transmitting
huge quantities of data,
• uses computers whenever appropriate.
• bioinformatics refers to computational
bioinformatics.
22

Bioinformatics
• an interdisciplinary field that develops
– methods and software tools for understanding
biological data
• combines
– computer science,
– statistics,
– mathematics,
– engineering
to analyze and interpret biological data
23

• has been used for in silico analyses of
biological queries using mathematical and
statistical techniques.
• [In silico (Latin for "in silicon") is an expression used to
mean "performed on computer or via computer
simulation.]
• primary goal is to increase the understanding
of biological processes.
• focuses on developing and applying
computationally intensive techniques to
achieve this goal.
24

• Techniques used include
– pattern recognition, data mining, machine learning
algorithms, and visualization
• Analyzing biological data to produce
meaningful information involves writing and
running software programs that use algorithms
from
– graph theory, artificial intelligence, soft computing,
data mining, signal processing, image processing,
and computer simulation.
25

• The algorithms in turn depend on theoretical
foundations such as
– discrete mathematics
– control theory
– system theory
– information theory
– statistics
26

27
...What is Bioinformatics?...
• Bioinformatics derives knowledge from computer
analysis of biological data that can consist of the
information stored in the
– genetic code,
– experimental results from various sources,
– patient statistics,
– scientific literature.
• Research in bioinformatics includes method
development for
– storage,
– retrieval,
– analysis
of the data.

28
...What is Bioinformatics?...
• Bioinformatics
– a rapidly developing branch of biology
– highly interdisciplinary,
– using techniques and concepts from
• informatics,
• statistics,
• mathematics,
• chemistry,
• biochemistry,
• physics,
• linguistics.

29
• The relationship between computer science and
biology is a natural one for several reasons.
– 1st,
• the phenomenal rate of biological data being produced provides
challenges:
– massive amounts of data have to be stored, analysed, and made accessible.
– 2nd,
• the nature of the data is often such that a statistical method, and
hence computation, is necessary.
– This applies in particular to the information on the building plans of proteins
and of the temporal and spatial organisation of their expression in the cell
encoded by the DNA.
– 3rd,
• there is a strong analogy between the DNA sequence and a
computer program
– it can be shown that the DNA represents a Turing Machine.

• The Turing Machine
– can simulate any
computing system
– consists of three basic
elements:
• a control unit,
• a tape,
• a read-write head.
The Turing Machine
30
• The read-write head moves along the tape and
transmits information to and from the control unit.

• DNA is transcribed to
messenger RNA in the cell
nucleus, which is in turn
translated to protein in the
cytoplasm.
• The Central Dogma, shown
here from a structural
perspective, can also be
depicted from an
information flow
perspective
The Central Dogma of Molecular Biology
31

32
Path to the Bioinformatics
– 1st,
• Learn Biology.
– 2nd,
• Decide and pick a problem that interests you for experiment.
– 3rd,
• Find and learn about the Bioinformatics tools.
– 4th,
• Learn the Computer Programming Languages.
– Perl, Pyton, R, Java, etc.
– 5th,
• Experiment on your computer and learn different programming
techniques.

33
Why is Bioinformatics Important?
• Applications areas include
– Medicine
– Pharmaceutical drug design
– Toxicology
– Molecular evolution
– Biosensors
– Biomaterials
– Biological computing models
– DNA computing

34
What skills are needed?
• Well-grounded in one of the following areas:
– Computer science
– Molecular biology
– Statistics
• Working knowledge and appreciation in the
others!

35
Scope of Computational Biology
Computational
Biology
Bioinformatics
Genomics
Proteomics
Functional
genomics
Structural
bioinformatics

• The study of the genome,
– which is the complete set of the genetic material or
DNA present in an organism.
• studies all genes and their inter relationships in an
organism to identify their combined influence on
its growth and development.
• The field of genomics attracted worldwide
attention in the late 1990s with the race to map the
human genome.
– The Human Genome Project (HGP), completed in
April 2003, made available for the first time the
complete genetic blueprint of a human being.
Genomics
36

• large-scale study of proteomes,
– which is a set of proteins produced in an organism,
system, or biological context.
• We may refer to, for instance, the proteome of a species (eg,
Homo sapiens) or an organ (eg, the liver).
– The proteome is not constant;
• it differs from cell to cell and changes over time.
– To some degree, the proteome reflects the underlying
transcriptome.
• However, protein activity (often assessed by the reaction rate
of the processes in which the protein is involved) is also
modulated by many factors in addition to the expression level
of the relevant gene.
Proteomics
37

• is used to investigate:
– when and where proteins are expressed;
– rates of protein production, degradation, and steady-state abundance;
– how proteins are modified (for example, post-translational
modifications (PTMs) such as phosphorylation);
– the movement of proteins between subcellular compartments;
– the involvement of proteins in metabolic pathways;
– how proteins interact with one another.
• can provide significant biological information for many biological
problems, such as:
– Which proteins interact with a particular protein of interest (for
example, the tumor suppressor protein p53)?
– Which proteins are localized to a subcellular compartment (for example,
the mitochondrion)?
– Which proteins are involved in a biological process (for example,
circadian rhythm)?
Proteomics
38

• is the branch of bioinformatics
– which is related to the analysis and prediction of the
three-dimensional structure of biological
macromolecules such as proteins, RNA, and DNA.
• deals with generalizations about macromolecular
3D structure such as comparisons of overall folds
and local motifs, principles of molecular folding,
evolution, and binding interactions, and
structure/function relationships, working both
from experimentally solved structures and from
computational models.
Structural bioinformatics/genomics
39

• is a field of molecular biology,
– which attempts to make use of the vast wealth of data
given by genomic and transcriptomic projects (such as
genome sequencing projects and RNA sequencing) to
describe gene (and protein) functions and interactions.
• Unlike structural genomics, it focuses on the dynamic aspects
such as gene transcription, translation, regulation of gene
expression and protein–protein interactions, as opposed to the
static aspects of the genomic information such as DNA
sequence or structures.
• attempts to answer questions about the function of
DNA at the levels of genes, RNA transcripts, and
protein products.
Functional genomics
40

41
Why is bioinformatics hot?
• Supply/demand: few people adequately trained in
both biology and computer science
• Genome sequencing, microarrays, etc lead to large
amounts of data to be analyzed
• Leads to important discoveries
• Saves time and money

42
The Role of Computational Biology
1 2 3 5 10 16 24 35 49 72 101 157
217
385
652
1.160
2.009
3.841
0
500
1.000
1.500
2.000
2.500
3.000
3.500
4.000
Millions
82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99
Source: GenBank
3D Structures
Growth:
Source:
http://www.rcsb.org/pdb/holdings.html
GenBank BASEPAIR GROWTH

43
Fighting Human Disease
• Genetic / Inherited
– Diabetes
• Viral
– Flu, common cold
• Bacterial
– Meningitis, Strep throat

44
Drug Development Life Cycle
Years
0 2 4 6 8 10 12 14 16
Discovery
(2 to 10 Years)
Preclinical Testing
(Lab and Animal Testing)
Phase I
(20-30 Healthy Volunteers used to
check for safety and dosage)
Phase II
(100-300 Patient Volunteers used to
check for efficacy and side effects)
Phase III
(1000-5000 Patient Volunteers
used to monitor reactions to
long-term drug use)
FDA Review
& Approval
Post-Marketing
Testing
$600-700 Million!
7 – 15 Years!

45
Drug lead screening
5,000 to 10,000
compounds screened
250 Lead Candidates in
Preclinical
Testing
5 Drug Candidates
enter Clinical Testing;
80% Pass Phase I
One drug approved by the FDA
30%Pass Phase II
80% Pass Phase III

• In the biotechnology industry, every researcher and
entrepreneur hopes to develop or discover the next "killer
app“
– the one application that will bring the world to his or her door
and provide funding for R&D, marketing, and production.
• For example, in general computing, the electronic spreadsheet and the
desktop laser printer have been the notable killer apps.
• The spreadsheet not only transformed the work of accountants, research
scientists, and statisticians, but the underlying tools formed the basis for
visualization and mathematical modeling.
• The affordable desktop laser printer created an industry and elevated the
standards of scientific communications, replacing rough graphs created
on dot-matrix printers with high-resolution images.
Killer application
46

• "What might be the computer-enabled 'killer app' in
bioinformatics?“
• Although there are numerous military and agricultural
opportunities, one of the most commonly cited examples of the
killer app is in personalized medicine, as illustrated in Figure
Killer application
47
personalized medicine:
the custom, just-in-time
delivery of medications
(popularly called "designer
drugs") tailored to the
patient‘scondition.

• Instead of taking a generic or over-the-counter drug for a
particular condition, a patient would submit a tissue sample,
such as a mouth scraping, and submit it for analysis.
– A microarray would then be used to analyze the patient's genome and
the appropriate compounds would be prescribed.
• The drug could be a cocktail of existing compounds, much like
the drug cocktails used to treat cancer patients today.
• Alternatively, the drug could be synthesized for the patient's
specific genetic markers—as in tumor specific chemotherapy,
for example.
– This synthesized drug might take a day or two to develop, unlike the
virtually instantaneous drug cocktail.
– The tradeoff is that the drug would be tailored to the patient's genetic
profile and condition, resulting in maximum response to the drug, with
few or no side effects.
Killer application
48

• How will this or any other killer app be realized?
– The answer lies in addressing the molecular biology,
computational, and practical business aspects of proposed
developments such as custom medications.
• A practical system would include:
– High throughput screening
• The use of affordable, computer-enabled microarray technology to
determine the patient's genetic profile. The issue here is
affordability, in that microarrays costs tens of thousands of dollars
Killer application
49

– Medically relevant information gathering
• Databases on gene expression, medical relevance of signs and
symptoms, optimum therapy for given diseases, and references for
the patient and clinician must be readily available.
• The goal is to be able to quickly and automatically match a patient's
genetic profile, predisposition for specific diseases, and current
condition with the efficacy and potential side effects of specific
drug-therapy options.
– Custom drug synthesis
• The just-in-time synthesis of patient-specific drugs, based on the
patient's medical condition and genetic profile, presents major
technical as well as political, social, and legal hurdles.
• For example, for just-in-time synthesis to be accepted by the FDA,
the pharmaceutical industry must demonstrate that custom drugs can
skip the clinical-trials gauntlet before approval.
Killer application
50

• Achieving this killer app in biotech is highly
dependent on
– computer technology,
• especially in the use of computers to speed the process
testing-analysis-drug synthesis cycle, where time really
is money.
• For example, consider that for every 5,000
compounds evaluated annually by the U.S.
pharmaceutical R&D laboratories, 5 make it to
human testing, and only 1 of the compounds
makes it to market.
Killer application
51

• In addition, the average time to market for a
drug is over 12 years,
– including several years of pre-clinical trials
followed by a 4-phase clinical trial.
• These clinical trials progress from
– safety and dosage studies in Phase I,
– to effectiveness and side effects in Phase II,
– to long-term surveillance in Phase IV,
with each phase typically lasting several years.
Killer application
52

• Most pharmaceutical companies view
computerization as the solution to creating smaller
runs of drugs focused on custom production.
• Obvious computing applications range from
– predicting efficacy and side effects of drugs based on
genome analysis,
– to visualizing protein structures to better understand and
predict the efficacy of specific drugs,
– to illustrating the relative efficacy of competing drugs in
terms of quality of life and cost, based on the Markov
simulation of likely outcomes during Phase IV clinical
trials.
Killer application
53

Bioinformatics Software: Two Cultures
54
Web-based or
graphical user interface (GUI)
Command line (often Linux)
Central resources
(NCBI,
EBI,)
Genome browsers
(UCSC, Ensembl)
Biopython,
Python, BioPerl, R:
manipulate data files
Next generation
sequencing tools
Data analysis
software: sequences,
proteins, genomes
GUI software
(Partek, MEGA,
RStudio,
BioMart,
IGV)
Galaxy
(web access
to NGS tools,
browser data)

• Many bioinformatics tools and resources are
available on the internet, such as major genome
browsers and major portals (NCBI, Ensembl,
UCSC).
• These are:
– accessible (requiring no programming expertise)
– easy to browse to explore their depth and breadth
– very popular
– familiar (available on any web browser on any
platform)
55

• Many bioinformatics tools and resources are
available on the command-line interface
(sometimes abbreviated CLI).
– These are often on the Linux platform (or other Unix-
like platforms such as the Mac command line).
– They are essential for many bioinformatics and
genomics applications.
– Most bioinformatics software is written for the Linux
platform.
• Many bioinformatics datasets are so large (e.g. high
throughput technologies generate millions to billions or even
trillions of data points) requiring command-line tools to
manipulate the data.
56

• Should you learn to use the Linux
operating system?
– Yes, if you want to use mainstream
bioinformatics tools.
• Should you learn Python or Perl or R
or another programming language?
– It’s a good idea if you want to go
deeper into bioinformatics, but also, it
depends what your goals are.
– Many software tools can be run in
Linux on the command-line without
needing to program.
• Think of this figure like a map.
– Where are you now?
– Where do you want to go?
CLI
57
Web-based or
graphical user interface (GUI)
Command line (often Linux)
Central resources
(NCBI,
EBI,)
Genome browsers
(UCSC, Ensembl)
Biopython,
Python, BioPerl, R:
manipulate data files
Next generation
sequencing tools
Data analysis
software: sequences,
proteins, genomes
GUI software
(Partek, MEGA,
RStudio,
BioMart,
IGV)
Galaxy
(web access
to NGS tools,
browser data)

Some web-based (GUI) and command-line (CLI) software
58

Some web-based (GUI) and command-line (CLI) software
59

• Many informatics disciplines have emerged in recent
years.
• Bioinformatics is distinguished by its particular focus
on DNA and proteins (impacting its databases, its tools,
and its entire culture).
Tool makers and tool users across informatics disciplines
60

• In addition to available books and courses, many websites
offer online training in the forms of tutorials or courses.
• Rules for online learning include:
– make a plan;
– be selective;
– organize your learning environment;
– do the readings;
– do the exercises;
– do the assessments;
– exploit the advantages (e.g., convenience);
– reach out to others;
– document your achievements;
– be realistic about your expectations for what you can learn.
• These rules also apply to reading a textbook.
Learning Programming for Bioinformatics
61
https://doi.org/10.1371/journal.pcbi.1002631

• Science by its nature is cumulative and
progressive.
• Whether you use web‐based or command‐line
tools, research should be conducted in a way
that is reproducible by the investigator and by
others.
• This facilitates the cumulative, progressive
nature of your work.
Reproducible Research in Bioinformatics
62

• A workflow should be well documented.
– This may include keeping text documents on your
computer in which you can copy and paste complex
commands, URLs, or other forms of data.
• To facilitate your work, information stored on a
computer should be well organized.
• Data should be made available to others.
– Repositories are available to store high-throughput data
in particular.
• Examples are Gene Expression Omnibus (GEO) and Sequence
Read Archive (SRA) at NCBI and ArrayExpress and European
Nucleotide Archive (ENA) at EBI.
63

• Metadata can be equally as crucial as data.
– Metadata refers to information about datasets.
• For a bacterial genome that has been sequenced, the metadata may
include the location from which the bacterium was isolated, the culture
conditions, and whether it is pathogenic.
• Databases that are used should be documented.
– Since the contents of databases change over time, it is
important to document the version number and the date(s) of
access.
• Software should be documented.
– For established packages, the version number should be
provided.
• Further documenting the specific steps you use allows others to
independently repeat your analyses.
– In an effort to share software, many researchers use repositories such as GitHub.
64

• Bioinformatics
• Genomics
• Transcriptomics
• Proteomics
• Epigenetics
• Interactomics
• Protein Analysis and Structure Prediction
• Next Generation Sequencing Technology
• Comparative Sequence Analysis
• Systems Biology
• Text Mining and Information Extraction
Bioinformatic Research Areas
65

66
Where Can I Learn More?
• ISCB: http://www.iscb.org/
• NBCI: http://ncbi.nlm.nih.gov/
• http://www.bioinformatics.org/
• Books
• Journals
• Conferences

67
Where Can I Learn More?
• https://www.codeschool.com/
• https://www.codecademy.com/
• https://www.datacamp.com/
• https://software-carpentry.org/
• https://github.com/

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