COMPARATIVE STUDIES ON NUTRITIONAL VALUE OF NORMAL AND TUMOR TISSUE, SARDINELLA LONGICEPS (VALENCIENNES, 1847) FROM NAGAPATTINUM SOUTHEAST COAST OF INDIA

A. Gopalakrishnan -et al., IJSIT, 2013, 2(5),432-443

COMPARATIVE STUDIES ON NUTRITIONAL VALUE OF NORMAL AND
TUMOR TISSUE, SARDINELLA LONGICEPS (VALENCIENNES, 1847)
FROM NAGAPATTINUM SOUTHEAST COAST OF INDIA
A. Gopalakrishnan*, K. Sinduja and A. Sakthivel
Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai
– 608 502, Tamil Nadu – India

ABSTRACT
Fish are at present in high demand in food markets, they are widely consumed in many parts of the
world because they posses high protein content, saturated fat and also contain omega fatty acids known to
support good health. The present study deals with biochemical composition of common fish,Sardinella
longiceps. The proximate composition of protein, carbohydrate, lipid, amino acids and fatty acids were
studied. The results of proximate composition in S. longiceps showed the percentage of protein was high in
the normal and tumor infected fish tissue (29.15 &18.93%), followed by the carbohydrate (5.81 & 2.42 %)
and lipid (15.61 & 9.28 %). The percentage compositions of essential and non-essential amino acids are
presented in normal tissue and tumor infected tissue were found to be as 46.09 % & 41.47 % and 37.23% &
40.63%. In the analysis, the fatty acid profile by gas chromatography revealed the presence of higher amount
of PUFA (Linolenic acid 32.74 %) in normal tissue. The details of the vitamins detected in S. longicepstissue.
Among them, vitamin A was found in higher levels (91.16 mg/gm) at normal tissue. In the present study,
totally 5 macro minerals and 2 trace minerals were reported. The S. longiceps normal and infected tissue
contributed maximum sodium (289.6 mg/gm) and Potassium (166.5 mg/gm) of minerals. The result shows
that marine fish (S. longiceps) tissue is a valuable food recipe for human consumption, due to its high quality
protein and well-balanced amino acids fatty acids and vitamins and minerals.
Key words: Marine fish, S. longiceps, proximate composition, amino acids, fatty acids, vitamins and minerals.

IJSIT (www.ijsit.com), Volume 2, Issue 5, September-October 2013

432


INTRODUCTION
The knowledge on biochemical composition of any edible organisms is extremely important since
the nutritive value is reflected in its biochemical contents [1]. A new species should be recommended for
human consumption only after assessing the nutritive value of the species with regards to its nutritional
qualities [2]. The demand for protein rich food is increasing, especially in developing countries, stimulating
the exploration of unexploited or non-traditional resources. Marine fish are commercially valuable species
and easy to cultivate in coastal areas. Marine fish are important for marine ecology and human diet, since it
is an important source of nutrients. Consumption of marine fish provides an inexpensive source of protein
with a high biological value, essential minerals and vitamins. Additionally, the fish muscle contains little
saturated fat and significant amount of Vitamin C. Fish is also a good source of minerals such as calcium,
potassium, zinc, iron, phosphorus and copper.
Protein is the essential substance of life and accordingly exists in the largest quantity of all
nutrients as a component of the living beings. The ratio of carbohydrate was less when compare to the other
nutrients such as proteins and lipids in animal tissues, especially in aquatic animals [3]. Lipids are the major
sources of metabolic energy and essential materials for the formation of cell and tissue membranes [4]. Fish
proteins are rich in essential amino acids (EAA). They are required for the maintenance of growth,
reproduction and synthesis of vitamins. Aquatic animal fats are good sources of essential fatty acids that are
not synthesized in the human body. Fatty acids in fish oil have a very distinctive character compared to fatty
acids from other sources. They consist not only essential fatty acids, but also a significant source of omega-3
fatty acids-especially eicosapentaenoic acid (EPA, C20:5n3) and docosahexanoic acid (DHA, C22:6n3). These
fatty acids play a vital role in human nutrition, disease prevention and health promotion [5]. Vitamins are
organic compounds representing a minor fraction in the day today life. Fish is a good dietary source of
vitamin B complex, Niacinamide and Folic acid. Fish is a good source of vitamins A and D; fatty fish have
higher level of vitamins than the lean fish. Vitamin content may be considerably influenced by methods of
handling, storage and preparation of sea food [6]. Seafood includes the following important macro minerals:
Ca, P, Mg and the electrolytes (Na and K). Trace minerals present in marine organisms are Mn, Fe, Co, Cu, Zn,
Ni, Mo and Cr (essential), Al, Ti, V and Ag (non-essential) and Pb and Cd (toxic). Marine organisms are good
sources of I, Ca and P which ranged from 70 to 80% in the skeleton of fishes.
As the world population is growing, the per capita consumption of seafood is also increasing
rapidly. Because of health consciousness, the modern day man is interested in taking seafood more in view
of its nutritional superiority than all other sources of food accessible to him. There remain no considerable
comparative studies on normal and infected tumor fish with regard to their nutritive value. Hence, the
present work was planned to study the proximate composition of S. longiceps through estimating their
major biochemical components such as total protein, carbohydrate and lipid content in the whole body
tissue apart from the amino acids, fatty acids profile, vitamins and minerals content.


433


MATERIALS AND METHODS
The marine normal and tumor infected fishS. longiceps were collected from the Nagapattinum (Lat.
110 15’ N Long. 790 46’ E) Southeast coast of India.S. longiceps is purely marine and estuarine habited. They
were brought to the laboratory, the animal body was removed and tissue were dried at 55 ºC in an oven and
used for biochemical analysis. The proximate compositions of the experimental samples were determined
by using standard methods; viz., protein [7], carbohydrate [8] and lipid [9]. The experimental fish samples
were dried at 55ºC for 24 hours in an oven and the dried samples were finely ground for estimating the
amino acids in the HPLC (Merck Hitachi L-7400) following the method of [10]. For fatty acid analysis, the
samples (body tissue) were homogenized with chloroform: methanol (2:1 v/v) mixture and the samples
were extracted using the method of [11]. After the fat was extracted, it was esterified with 1% H 2SO4 and
fatty acid methyl esters were prepared by following the procedure of [12]. Identification and quantification
of fatty acids were done using Gas chromatography (Hewlett Packard 5890 model). The vitamins were
estimated the fat soluble vitamins A, D, E and K and the water soluble vitamins B1, B2, B6, B12 and C were
analysed in the HPLC (Merk Hitachi L-74000) following the method described by [13]. The folic acid was
estimated by following the calorimetric procedure of [14]. The pyridoxine, panthothenic acid and vitamin
B12 were estimated by following methods suggested in USP NF 2000 Asian edition. The minerals were
estimated soft tissue S. longicepsby following the method of [15].

RESULTS
The proximate composition (%) such as protein, carbohydrate and lipid contents ofS.
longicepstissue (normal and non- infected) arepresented. The results of the present study revealed that the
protein composition were high (29.15 &18.93%), followed by carbohydrate (5.81 & 2.42 %) and lipid
(15.61 & 9.28 %). The percentage compositions of essential and non-essential amino acids are presented in
normal tissue (Table 1). The total essential amino acids were found to be as 46.09 % and non-essential
amino acids were 41.47 %. Among the essential amino acids Phenylalanine were found high as 11.64 % on
dry matter basis in fish tissue and the non-essential amino acids Glutamate were found maximum as 8.15 %
(Table1). The percentage compositions of essential (37.23%) and non-essential amino acids (40.63%) are
presented in tumor infected tissue (Table 2). Among the essential and non essential amino acidsThreonine
&Aspartate were found high as 8.47 &9.96 % on dry matter basis in fish infected tissue (Table 2).


434


Figure 1: Showed the normal and tumor infected fish
EAA

% of amino acids

NEAA

% of amino acids

Phenylalanine

12.64

Glycine

6. 87

Lysine

5.14

Serine

9. 16

Histidine

4.65

Glutamic acid

3.95

Methionine

6.72

Cystine

2.63

Arginine

1.81

Glutamate

5.23

Leucine

6.27

Alanine

1.62

Threonine

4.25

Proline

0.48

Isolucine

2.94

Aspartate

3.82

Valine

1.83

Tyrosin

5. 95

Tryptophan

0.65

Aspertic acid

1.76

Table 1. Essential and non essential amino acids of normal tissue


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EAA

% of amino acids

NEAA

% of amino acids

Phenylalanine

2.77

Glycine

3.53

Lysine

5.82

Serine

6.14

Histidine

6.03

Glutamic acid

5.63

Methionine

2.19

Cystine

4.75

Arginine

0.12

Glutamate

3.15

Leucine

3.58

Alanine

0.83

Threonine

8.47

Proline

1.85

Isolucine

4.45

Aspartate

9.96

Valine

3.52

Tyrosin

1.77

Tryptophan

0.28

Aspertic acid

3.02

Table 2: Essential and non essential amino acids of tumor infected tissue
In S. longiceps, 7 different fatty acids were found in normal tissue; they are three saturated fatty
acids (36.67), one monounsaturated fatty acids (14.84) and three polyunsaturated fatty acids (43.48).
Among the PUFA Linolenic acid were the major acids (Table.3). The percentage availability of SFA, MUFA
and PUFA content was 27.09, 9.48 & 43.11 % in infected tissue (Table.4). In Linolenic acid was the major
acids found.
S. No

Fatty acids

Carbon atom (n)

% of fatty acids

Saturated Fatty Acids
1

Palmitic acid

C16:0

22.54

2

Margaric acid

C17:0

3.61

3

Stearic acid

C18:0

10.52

Total

36.67

Mono Unsaturated Fatty Acids
4

Oleic acid

C18:1

Total

14.84
14.84

Poly Unsaturated Fatty Acids
5

Linolenic acid

C18:3

32.74

6

Alpha Linolenic acid

C18:3

2.43

7

Stearidonic or Moroctic acid

C18:4

8.31

Total

43.48
Table 3: Fatty acid profile of normal


436

S. No

Fatty acids

Carbon atom (n)

% of fatty acids

Saturated Fatty Acids
1

Palmitic acid

C16:0

16.45

2

Margaric acid

C17:0

3.08

3

Stearic acid

C18:0

8.37

Total

27.09

Mono Unsaturated Fatty Acids
4

Oleic acid

C18:1

Total

9.48
9.48

Poly Unsaturated Fatty Acids
5

Linolenic acid

C18:3

26.58

6

Alpha Linolenic acid

C18:3

7.72

7

Stearidonic or Moroctic acid

C18:4

5.81

Total

43.11
Table 4: Fatty acid profile of infected fish

The details of the vitamins detected in normal and infected tissueare presented in Table 5. Among them,
vitamin A were found in higher levels, whereas vitamin BE were noticed as lower levels in normal tissue
(0.17) and vitamin A was high in infected tissue.
S. No

Vitamins

Normal

Infected

1

Retinol (A)

91.16

38.1

2

Calciferol (D)

5.89

2.18

3

Tocopherol (E)

0.17

0.11

4

Vitamin (K)

5.48

0.58

5

Pyridoxin (B6)

2.56

0.54

6

Cobalamin (B12)

4.08

9.74

7

Vitamin (C)

6. 23

2.86

Table 5:Vitamin content of S. longiceps(mg/g of the sample)
The quantity of minerals present in the tissue samples (Table.6). Totally, 5 macro minerals and 2
trace minerals were detected. Among the macro minerals, sodium (289.6), potassium (166.5 mg/g) and
copper (2.56 mg/g) were observed at higher and lower levels in normal tissue, whereas other macrominerals magnesium and calcium were in negligible level. The trace minerals such as iron (3.07 mg/g)
and zinc (0.24 mg/g) were also detected. The macro minerals, Potassium (102.7 mg/g) were observed at


437

higher levels in infected tissue.
S. No

Minerals

Normal

Infected

Macro
1

Calcium

35.9

56.47

2

Sodium

289.6

89.64

3

Potassium

166.5

4

Copper

2.56

8.45

5

Magnesium

21.6

35.25

6

Iron

3.07

8.23

7

Zinc

0.24

0.37

142.7

Trace

Table 6:Minerals content of S. longiceps(mg/g of the sample)

DISCUSSION
Fish is a major source of animal protein and it also contains vitamins. Fish is widely consumed in
many parts of the world by humans because it has high protein content, low saturated fat and also contains
omega fatty acids known to support good health. Marine foods are very rich sources of mineral components.
The total content of minerals in the raw flesh of marine fish and invertebrates is in the range of 0.6–1.5%
wet weight. Mineral components such assodium, potassium, magnesium, calcium, iron, phosphorus and
iodine are important for human nutrition [16]. Hence, the present work was planned to study the proximate
composition of S. longiceps through estimating their major biochemical components such as total protein,
carbohydrate and lipid content in the whole body tissue apart from the amino acids, fatty acids profile,
vitamins and minerals content.
Biochemical components such as protein, carbohydrates and lipids are essential for body growth
and maintenance. Protein is essential for the sustenance of life and exists in largest quantity of all nutrients
as a component of the human body [17]. The present investigation revealed that the maximum level of
protein content in normal and infected tissue is 29.15 &18.93 %. Similarly,[18] reported that the protein
(%) 19.41 ± 1.44, 19.11 ± 1.63 and 17.86 ± 1.14 is the major biochemical component in the Atherina boyeri,
Atherina lagunae and Atherina sp. [19]

estimated maximum level of (17.9%) of protein in Gadus

macrocephalus. Agreeing to the above the normal and tumor infected of S. longiceps in the present study
also exhibited variations in protein content in tissue. In the present study the percentage of carbohydrates
in the body tissue of 5.81 & 2.42 %. [20] reported that the carbohydrate 2.12-4.82%, 1.14-3.92%, 2.144.75% and 1.55-5.48% is the major biochemical component in the Sardinella longicephs, Rastrelliger
kanagurta, Lates calcarifer andEpinephelus tauvina. In S. longiceps, generally the carbohydrate content was


438

found to be low as it is high when compared to other fish. The lipids are highly efficient as source of energy,
in that they contain more than twice the energy of carbohydrate and proteins. Spawning in oil sardine is
prolonged and fishes with gravid gonads were observed throughout the year [21]. In the present study lipid
content of tissue were15.61 & 9.28 %. [22] Who studied to lipid composition in the lantern fish Benthosema
Pterotum found that for protein fat 3.4%. [23] reported that the lipids constituents from the ovary of S. niger
at maturation III and IV stage is high value 11.83 ± 1.00 &16.33 ± 1.04 %.Lipids comprise only (20%) dry
weight of their ovaries [24]. In the present study, the high lipid values were observed in normal tissue.
Biological value of protein is obviously reflected upon its essential amino acids concentration. In
general, the finfishes have a balanced distribution of all essential amino acids required for an adult per day.
The percentage compositions of essential and non-essential amino acids are presented in normal tissue. The
total essential amino acids were found to be as 46.09 % and non-essential amino acids were 41.47 %. Among
the essential amino acids Phenylalanine were found high as 11.64 % on dry matter basis in fish tissue and the
non-essential amino acids Glutamate were found maximum as 8.15 %. The percentage compositions of
essential (37.23%) and non-essential amino acids (40.63%) are presented in tumor infected tissue. Among
the essential and non essential amino acidsThreonine &Aspartate were found high as 8.47 &9.96 % on dry
matter basis in fish infected tissue. [20] reported that the R. Kanagurtta, L. Calcorifer, E.tauvina was essential
amino acids and non essential amino acids 50.24 & 42.5, 52.72 & 42.46 and 48.44 & 44.87%. [25] analysed
amino acid and fatty acid nutritional quality of muscles of Pampus sp and they reported that Pomfret protein
contained a high amount of glutamic acid. [26] reported that the higher amount of glutamic acid in all their
four species (Clupea harengus, Scomber scombrus, Trachurus trachurus and Urophycis tenuis) studied. [27]
reported that the higher amount of EAA and NEAA in Myrophis platyphynchus in which the amino acids range
from 47.28% and 52.72%. Leucine is generally high in fish protein [28] relatively low values were reported in
Indian shark, Silver belly and sole.In the present study, fish value of Phenylalanine was noticed high in normal
tissue and low value in infected tissue. The result revealed in this study showed that moonfish,S. longiceps
tissue is a potential source for food value due to high quality protein, as well as balanced essential amino
acids.
In S. longiceps, 7 different fatty acids were found in normal tissue; they are three saturated fatty
acids (SFA), one monounsaturated fatty acids (MUFA) and three polyunsaturated fatty acids (PUFA). Among
the PUFA Linolenic acid were the major acids. The percentage availability of SFA, MUFA and PUFA content
was 36.67, 14.84 & 43.48 % in S. longiceps. The percentage availability of SFA, MUFA and PUFA content was
27.09, 9.48 & 43.11 % in infected tissue. In Linolenic acid was the major acids found.[18] contributed that
the total monounsaturated fatty acids (especially C16:1 and C18:1) were more abundant inAtherina
lagunae, A. boyeri and Atherina sp. (36.2, 25.37 and 32.65% respectively). [29] reported that the the amount
of total lipids and fatty acids both in muscle and liver, varied by months and seasons (P<0.05).[30] reported
that the Palmitic acid showed its higher level in both species (5.78 and 3.89%) in Dussumieria acuta and
Sardinella brachysoma, respectively. [31] the percentages of polyunsaturated fatty acids (PUFA) were


439

higher than the percentages of saturated fatty acids (SFA) and double the percentages of monounsaturated
fatty acids (MUFA). [32] studied that the nutritional value of Stolephours waitei, Chirocenttrus nudus and
Pomadasys kaakan its suitability for consumption. In which the SFA, MUFA and PUFA fatty acids range from
35.87 to 0.39%, 28.74 to 0.41% and 18.81 to 0.42% respectively. The main dominant PUFA was DHA
(22:6n-3), followed by EPA (20:5n-3), AA (20:4n-6), LA (18:2n-6) and ALA (18:3n-3). These results are in
agreement with studies of fatty acids composition of fishes [33]. The PUFA content in the case of marine fish
ranges from 28 to 57% with C20:5 and C22:6 predominating and constituting about 50% in most cases [34].
In the present study, S. Longiceps showed the dominance of linolenic acid (C18:3) of polyunsaturated fatty
acids in normal tissue.
Vitamins are organic chemical compounds essential for promoting growth, reproduction and
maintenance of normal body health and function. [35] Fish liver oils are the richest sources of vitamin A and
D, and the flesh is rich in vitamin B complex, especially niacin and B12. [6] stated that the flesh of fish and
shell fish flesh in nut considered to be important sources of vitamin A while high of at fishes (e.g. eel,
mackerel end menhaden) contain moderate amounts. The details of the vitamins detected in normal and
infected tissue. Among them, vitamin A were found in higher levels, whereas vitamin BE were noticed as
lower levels in normal tissue (0.17) and vitamin A was high in infected tissue. [20] reported that soluble
vitamins A were more than D and E in S. Longiceps. In the present study, the vitamin A was more in S.
longicepsnormal fish tissue.
Minerals are the chemical elements, which are involved in the building of organisms and are
necessary for its proper functioning. [36] explained the importance of Ca, Mg, and K in the human nutrition.
The main aim of the present studies totally, 5 macro minerals and 2 trace minerals were detected. Among
the macro minerals, sodium (289.6), potassium (166.5 mg/g) and copper (2.56 mg/g) were observed at
higher and lower levels in normal tissue, whereas other macro-minerals magnesium and calcium were in
negligible level. The trace minerals such as iron (3.07 mg/g) and zinc (0.24 mg/g) were also detected. The
macro minerals, Potassium (102.7 mg/g) were observed at higher levels in infected tissue. The potassium
content of raw rainbow trout was found to be 306mg/100g [37]. [21] Reported that potassium content was
more in R. kanagurta followed by phosphorus and sodium. [38] Found the sodium content of individual
species of salt water fish to range from 39 to 96 mg/100g with the average of 68mg/100g. [35] Reported
that sea foods in general are excellent sources of I, Ca, P, Na, Fe, Zn and oysters are good sources of Fe and
Cu. When compared to the other fish, the calcium content of raw rainbow trout was found to be
63.2mg/100g [37]. In the present study normal tissue showed higher levels of sodium and potassium.
Consumption of finfishes especially S. longiceps tissue enriches of minerals.
In general, seafood is one of the most nutritionally balanced foods. The seafood diet helps to control
weight and goes a long way towards preventing heart diseases. Studies on fatty acid composition of


440

commercial seafood in India are limited. This might be due to lack of awareness on benefits of these
nutrients particularly from fish tissue. The nutritional values of fish are not brought to the limelight so far,
so consumption of these nutrient rich fish has not attracted attention. The results of the present study
provide information about the amino acids and fatty acid composition, but also suggest the consumption of
this fish tissue. It is rich in amino acids (Phenylalanine), fatty acids (linolenic acid) and minerals (sodium,
potassium). Further, the presence of amino acids (Phenylalanine) and fatty acids (linolenic acid) in S.
longiceps tissue adds more value through the possibility. From the above observation it is clear that the
normal tissue of S. longicepswith rich nutritive value can be used for alternate source as a regular sea food
which supplies nutrients for the growing children, pregnant women and people suffering from malnutrition.

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COMPARATIVE STUDIES ON NUTRITIONAL VALUE OF NORMAL AND TUMOR TISSUE, SARDINELLA LONGICEPS (VALENCIENNES, 1847) FROM NAGAPATTINUM SOUTHEAST COAST OF INDIA

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COMPARATIVE STUDIES ON NUTRITIONAL VALUE OF NORMAL AND TUMOR TISSUE, SARDINELLA LONGICEPS (VALENCIENNES, 1847) FROM NAGAPATTINUM SOUTHEAST COAST OF INDIA